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Citations for Thermo Fisher :
5401 - 5450 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... samples were washed with PBTX for three times and replaced with secondary antibody (1%, goat anti-mouse Alexa Fluor 488 and goat anti-rat Alexa Fluor 568, ThermoFisher UK) and 1 ug/ml DAPI solution ...
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bioRxiv - Plant Biology 2022Quote: ... the samples were washed three times with TBS-Tween 20 followed by incubation with 1:1000 secondary antibody in 2 % milk in TBS-Tween 20 for 1.5 hours (Anti-rat IgG for JIM5 and JIM7 and Anti-mouse IgG for 2F4 couples to Alexa-Fluor; Molecular Probes). The sections were washed three times with TBS-Tween 20 and then incubated with propidium iodide (20 mg ml−1 ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 488 and anti-mouse Alexa Fluor 568 (1/100, A-21470 and A-21124, ThermoFisher Scientific) for 45 min at R.T ...
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 488 and anti-mouse Alexa Fluor 568 (1/100, A-21470 and A-21124, ThermoFisher Scientific) for 1 h at R.T.
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bioRxiv - Molecular Biology 2023Quote: ... then incubated with anti-rat Alexa Fluor 568 and anti-mouse Alexa Fluor 488 (1/25, A11077 and A11001, ThermoFisher Scientific) for 45 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... including anti-rabbit or anti-mouse Alexa Fluor 568 and anti-rat Alexa Fluor 488 (all 1:1000, Thermo Fisher Scientific), were added and incubated for 2 h at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... and incubated for 2 hours at room temperature in the dark in goat anti-mouse IgG conjugated with Alexa-Fluor 488 or goat anti-rat IgG conjugated with Alexa-Fluor 488 (Molecular Probes, Life Technologies Corporation ...
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bioRxiv - Cell Biology 2023Quote: ... The slides were washed for 5 minutes 3 times with PBS followed by incubation with goat anti-Rat IgG (H+L) Alexa Fluor 594 secondary antibody (A11012, Thermo Fisher) at 1:500 dilution for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad], Secondary anti-Rat IgG, [ThermoFisher] ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 488-conjugated anti-mouse IgG (also cross-adsorbed to rabbit IgG and rat IgG) (A-11029; Thermo Fisher Scientific) and Alexa Fluor 568-conjugated anti-rabbit IgG (also cross-adsorbed to mouse IgG ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... The total dissociated single cells were resuspended in 500 μL sorting buffer (25 mM HEPES, 5 mM EDTA, 1% BSA, in HBSS) containing Normal Rat Serum (1:100; Invitrogen, 10710C) and TruStain FcX™ (anti-mouse CD16/32 ...
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bioRxiv - Developmental Biology 2022Quote: Cell sizes of mouse and rat embryonic molars were determined by staining 6 µm thick histological sections with DiI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Immunology 2022Quote: ... Control APC755 tetramers were added at a final concentration of 25 nM in the presence of 2% rat and mouse serum (ThermoFisher Scientific) and incubated at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated with secondary antibodies of goat-anti-rat 488 and goat-anti-rabbit 594 (1:500, Invitrogen, A11006, A11037) 1.5 hours in room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Wings were then incubated in 1:500 secondary antibody at the concentration 1:500 with anti-rat AF488 (Invitrogen, #A-11006) and anti-Guinea pig AF555 (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... followed by probing with secondary antibodies (goat anti-rabbit Alexafluor 488, A11034, 1:500; goat anti-rat Alexafluor 647, A21247, 1:500, ThermoFisher Scientific) applied and washed as per the primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed in PBS and incubated for 2 h at room temperature with secondary antibodies: goat anti-rat Alexa Fluor 555 (1/500; Invitrogen, #A21434), goat anti-rabbit Alexa Fluor 488 (1/500 ...
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bioRxiv - Molecular Biology 2023Quote: ... Alexa Fluor 568 goat anti-mouse IgG (H+L) and Alexa Fluor 488 goat anti-rat IgG (H+L) (both from Invitrogen Molecular Probes), for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... the coverslips were subjected to staining using the following secondary antibodies: goat anti-rat Alexa Fluor 594 (Thermo Scientific, #A-11007), goat anti-mouse Alexa Fluor 594 (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Incorporation of CldU was detected using rat-anti-BrdU antibodies (1:500; BU1/75, AbD Serotec) and Alexa fluor-555-labelled goat-anti-rat antibodies (1:500; Molecular Probes), whereas incorporated IdU was detected using mouse-anti-BrdU antibodies (1:750 ...
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bioRxiv - Cell Biology 2023Quote: ... blocked again and stained with secondary antibody (Goat-Anti-Rat conjugated to Cy3 AP136C, SigmaAldrich) and DAPI (D9542, SigmaAldrich) and then mounted with vectashield (1551231, Fisher Scientific,).
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bioRxiv - Cancer Biology 2023Quote: ... Sections were washed in DPBS for 10 min and incubated with secondary antibodies (Donkey anti-rabbit Life Technologies cat. no A31573, 1:400; Donkey anti-rat ThermoFisher Scientific cat ...
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bioRxiv - Cancer Biology 2023Quote: ... SCC25-Luc-GFP cells co-cultured with mouse or naked mole-rat fibroblasts were incubated with 10 µM 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher) for 1.5 hours ...
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bioRxiv - Immunology 2024Quote: ... we cultivated the corresponding rat hybridomas in IMDM (Gibco, 12440-053; supplemented with 10 % FCS, 1X MEM Non-essential amino acid solution (ThermoFisher, 11140050), 2 mM Glutamax (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were as follows: isotype-specific Alexa Fluor 488- and Alexa Fluor 546- conjugated goat anti-rat IgG antibodies (Thermo Fisher Scientific Cat# A-21434 ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 565 (1:500, anti-rat coupled to Alexa Fluor 546, Cat No. A11081, Fisher Scientific, Waltham, MA, USA) secondary antibodies diluted in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... we counted the number of TH+/PHOX2B+ and TH+/PHOX2B- cells along the ventrolateral medulla (8 sections/rat; 240 µm interval) using an EVOS fluorescent microscope (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: ... for 1h at RT and stained o/n with antibody fluorescent conjugates (1:500, anti-mouse-AF488, anti-rabbit-AF555 or 647, anti-rat- AF546, all from Thermo-Fisher Scientific), 1mg/ml DAPI solution 1:500 and PNA-lectin AF594 1:1000 (1mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Lung cell suspension was then subjected to positive sorting with an antibody against the endothelial cell marker ICAM-2 conjugated to magnetic Dynabeads Sheep Anti-Rat IgG (11035) from Thermo Fisher, for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: 12mm diameter coverslips in 24 well plate were coated with 50µg/ml rat tail Collagen I (Gibco, Catalog number: A10483-01) diluted in 0.02M acetic acid for 1 hour at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... and horseradish peroxidase-labeled goat anti-rat as the secondary antibody at a dilution of 1:5000 (Invitrogen, catalog no. 31470).
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bioRxiv - Pathology 2024Quote: ... tissues were washed in PBS and probed using a goat-anti-rat IgG 488 secondary antibody (Invitrogen A-11006, 1:500) for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times for 15 min in PBS before a 2 h incubation with either a donkey anti-rat secondary antibody conjugated with Alexa Fluor 488 (dilution 1:500, Invitrogen, #A21208) or with a goat anti-rat secondary antibody conjugated with Cy5 (dilution 1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... goat anti-rat Alexa 488 Invitrogen A11006; 1:500, goat anti-rabbit Alexa 555 Invitrogen A21429; 1:500 streptavidin Alexa 647 Invitrogen S32357). Washes in PBS with 0.2% Triton were performed three times at 5 ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from rats’ hearts with use of the Gene Jet PCR Purification Kit (Thermo Fisher Scientific, Waltham, MA) and quantified with a Nano-Drop One spectrophotometer (Thermo Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... the dissected tissues were incubated for 24 hours in secondary goat anti-rat IgG antibodies: Alexa Fluor 488 conjugated (Molecular Probes, Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: Hippocampal neurons were isolated from the Hippocampi of embryonic day 17–18 Sprague Dawley rats by dissociating in TrypLE™ Select Enzyme (Gibco) supplemented with 0.005% DNaseI in an incubator maintained at 37°C and 5% CO2 ...
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bioRxiv - Genomics 2024Quote: ... and incubated with either anti-rabbit or anti-rat secondary antibodies with AF594 (diluted 1:400, A11012 or A1107, Thermofisher Scientific) for 1 hour ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies used were conjugated Alexa Fluor 488 or 647 against rat or rabbit (ThermoFisher, A11008, A11006, A21245, A21247; 1:500) and conjugated Alexa Fluor 488 against chicken (Abcam ...
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bioRxiv - Synthetic Biology 2024Quote: ... 500 μl rat tail collagen I (50 μg/ml diluted in 25 mM acetic acid, Thermo Fisher Scientific, catalog no. A1048301) was added to each well for one hour at RT ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from 100-150 mg of flash-frozen rat somatosensory cortex tissue using TRIzol reagent (ThermoFisher Scientific #15596026) as previously described (Rio et al. ...
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bioRxiv - Biophysics 2019Quote: ... namely 1) epoxylated beads with a diameter of 4.5 μm and 20% iron oxide content (Dynabeads M-450, Invitrogen AG, Carlsbad, CA), and 2 ...
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bioRxiv - Genetics 2021Quote: Protein lysates from cell and tissues were lysed and homogenized (tissues) in Mammalian Protein Extraction Reagent (M-Per) lysis buffer (Cat# 78501; ThermoFisher; Waltham, MA) supplemented with cOmplete protease inhibitor tablets (Cat# 1183617000 ...
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bioRxiv - Genetics 2021Quote: ... Three µg of total RNAs was applied to synthesize the first strand of cDNA using M-MulV reverse transcriptase (Thermo Scientific, USA), oligo dT and random hexamer primers (MWG ...
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bioRxiv - Molecular Biology 2021Quote: ... Deselection of streptavidin-specific phages was performed before each round of selection using 80 µL of streptavidin-coated Dynabeads (Invitrogen, M-280). Additionally ...
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bioRxiv - Developmental Biology 2021Quote: DNA probes for the protein–DNA pull-down assay were generated by PCR using 5’-biotinylated primers and then conjugated to Dynabeads M-280 Streptavidin (Thermo Fisher Scientific). The recombinant proteins synthesized in HEK293T cells were extracted and incubated with the DNA-probe-conjugated beads in 0.5% NP-40 ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 500 μm disk patterns were coated with 10 μg/ml recombinant human laminin 521 (BioLamina) diluted in pre-warmed DPBS (Thermo Fisher Scientific) for 3 hours at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... 3 μg of total RNAs was applied to synthesize the first strand of cDNA using M-MulV reverse transcriptase (Thermo Scientific, USA) by oligo dT ...