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Citations for Thermo Fisher :
5451 - 5500 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Cancer Biology 2019Quote: ∼1 x 106 HEK293 cells grown at 70% confluency on 35 mm dish embedded with 12 mm glass viewing area (Nunc, Thermo Fisher Scientific Inc., Waltham, MA, USA) were transfected with appropriate plasmids using Lipofectamine 3000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Whole transcriptome RNA-Seq (wtRNA-Seq) libraries were prepared from total RNA (5 μg per sample) plus ERCC RNA Spike-In (Ambion® by Life Technologies, 4456739) followed by selective ribosomal RNA (rRNA ...
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bioRxiv - Immunology 2019Quote: ... cDNA libraries were prepared using 300 ng of total RNA and 2ul of a 3:1000 dilution of ERCC RNA Spike-In Controls (Ambion® Thermo Fisher Scientific, #4456739). The fragmented mRNA samples were subjected to cDNA synthesis using Illumina TruSeq® RNA sample preparation kit version 2 (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of 100 μM oligonucleotide bridge 2 and 2 units of T4 PNK (ThermoFisher) were added to 20 μl of the purified smDNA solution ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNA encoding the short transcriptional variant of human DA D2 receptor with an N-terminal FLAG tag (SF-D2R-S) was inserted into mammalian expression vector pcDNA 3.1(+) (Invitrogen)62 ...
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bioRxiv - Genetics 2020Quote: ... To generate donor plasmids the desired homology arm’s regions and epitope tag (either V5 or FLAG) were synthetized by Invitrogen GeneArt Gene Synthesis and cloned into the vector backbone pMK-RQ ...
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bioRxiv - Molecular Biology 2021Quote: ... and C-terminal foldon trimerization motif followed by an octa-histidine tag was cloned into a pcDNA3.1 expression vector (Invitrogen). Further ...
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bioRxiv - Molecular Biology 2020Quote: ... vector using Gateway cloning in frame with the C-terminal V5 tag (Gateway LR Clonase II mix; Life Technologies). The Gli2 P1-6A mutant was previously described (15) ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Developmental Biology 2019Quote: ... GenBank accession number BC057894.1) with an N-terminal hemagglutinin (HA) tag were reverse transcribed with SuperScript III Reverse Transcriptase (Invitrogen) and PCR-amplified using Phusion high-fidelity DNA polymerase (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: Desalted peptides from each sample (500μg) were labeled with 10-plex Tandem Mass Tag (TMT) reagents (ThermoFisher Scientific, 90110) (58) ...
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bioRxiv - Molecular Biology 2019Quote: ... These sequences and corresponding WT sequences were polymerase chain reaction (PCR) amplified (with the inclusion of a FLAG tag for REL sequences) and inserted into the pcDNA3.1(+) vector (Invitrogen) and pTRE-TIGHT vector (Takara Bio) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg of total peptides per condition were labeled with tandem mass tag (TMT10plex and TMT11, Thermo Fisher Scientific). After >98% TMT incorporation rate was confirmed ...
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bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
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bioRxiv - Biophysics 2021Quote: ... as specified by the commercial protocol) and a fluorescent tag (Alexa Fluor® succinimidyl esters, Invitrogen™, Molecular Probes® ...
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bioRxiv - Neuroscience 2020Quote: ... and both WT and mutant VAMP2 sequences were shuttled into Gateway™ pDEST™ 26 Vector (6xHis tag, Invitrogen). The in-frame V5-STX1a and 6xHis-(m)VAMP2 sequences were verified by DNA sequencing.
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bioRxiv - Immunology 2021Quote: ... Cell pellets were then incubated with a mouse Anti-V5 tag monoclonal antibody (1.2 mg mL−1; Thermofisher Scientific) in PBS 0.1% BSA for 45 min at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... the protein was expressed as a fusion construct with an N-terminal His6 tag followed by tobacco etch virus (TEV) protease cleavage site in E.coli BL21 DE3 Star cells (Invitrogen). The fusion construct was purified using immobilized-nickel affinity chromatography ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma triacylglycerol (TAG) and cholesterol were measured with colorimetric assays (TR22421 and TR13421, respectively, ThermoFisher Scientific, Middletown, VA, USA). Plasma ALT and AST concentrations were measured with kinetic spectrophotometric assays (A524-150 and A559-150 ...
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bioRxiv - Cancer Biology 2022Quote: ... RNAs were labeled with a desthiobiotin tag by using a Pierce RNA 3′ End Desthiobiotinylation Kit (20163; Thermo Scientific). Pull-down of RNA– protein complexes was performed using a Pierce™ Magnetic RNA-Protein Pull-Down Kit (20164 ...
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bioRxiv - Biochemistry 2022Quote: Bait and tag proteins (10-15 μg in 100 μL PBS) were immobilised in 96-well MaxiSorb plate (NUNC) o.n ...
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bioRxiv - Immunology 2022Quote: ... and C-terminal foldon trimerization motif followed by hexa-His tag) were used to transiently transfect Expi293F cells (Gibco). Four days after transfection ...
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bioRxiv - Biophysics 2022Quote: The human wild-type S1PR5 gene (UniProt ID Q9H228) with an N-terminal 3 × HA epitope (YPYDVPDYA) tag was cloned into pcDNA3.1+ (Invitrogen) at KpnI(5’ ...
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bioRxiv - Plant Biology 2022Quote: ... fused with an N-terminal 6×His-tag and cloned into expression vector pPIC9 (Thermo Fisher Scientific, California, USA). Correctness of the resulting constructs was confirmed by DNA sequencing prior to introduction into Pichia pastoris strain GS115 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Protein expression was confirmed by western blot using a 6x His-Tag HRP conjugated Monoclonal Antibody (Thermo Fisher Scientific). Once verified ...
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bioRxiv - Biochemistry 2021Quote: ... labelled with Tandem Mass Tag (TMT) six or ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific) and the labelled samples pooled.
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bioRxiv - Microbiology 2021Quote: ... a recodonized TgHypo cDNA sequence fused to a C-terminal 3×HA tag was synthesized by GeneArt (Life Technologies). This fragment was cloned into the BamHI and HindIII sites of a modified version of the parental plasmid p5RT70loxPKillerRedloxPYFP-HX3 ...
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bioRxiv - Microbiology 2020Quote: ... which also encodes 3x Flag tag was first cloned into a pBS SK vector (Thermo Fisher, Waltham, MA USA). The pEPkan-S plasmid was used as a source of the kanamycin cassette ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were incubated 1 mg L-1 of anti-6X-His tag monoclonal antibody [HIS.H8] with an HRP conjugate (ThermoFisher) suspended in 10 mL 1X TBST for 0.5 hours ...
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bioRxiv - Cancer Biology 2020Quote: The trigger peptides required for the TOMAHAQ method were generated by labeling with TMT-super heavy tags (TMTsh, +235.18, ThermoFisher) on the AssayMAP Bravo ...
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A quantitative tri-fluorescent yeast two-hybrid system: from flow cytometry to in-cellula affinitiesbioRxiv - Biochemistry 2019Quote: ... A second copy of the Tag-BFP (primSB_0120 and 121) was inserted in our new NheI site (Thermo Scientific), by homologous recombination to generate the pBFP2 plasmid ...
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bioRxiv - Biophysics 2020Quote: ... were cloned into the plasmid pCellFree_03 for expression with N-terminal GFP tag.30 Each plasmid (60 nM) was added to Leishmania tarentolae extract31,32 supplemented with RnaseOUT (1:1000, Invitrogen) and the mixture was incubated at 28 °C for 2.5 h.
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bioRxiv - Cell Biology 2020Quote: ... nucleotide fragments encoding the fusion Flag tag protein and full-length of Myocardin and encoding the fusion Myc tag protein and full-length of STAT3 were cloned to mammalian expression vector pCDNA3.1 (Invitrogen) (Flag-pCDNA3.1-Myocardin ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Enzyme coding sequences were tethered with a C-terminal HA tag in pFastBac Dual baculoviral vectors (Invitrogen, Waltham, MA) using Ph or p10 expression cassettes for hOR or other enzymes ...
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides were labeled using 11-plex or 16-plex tandem mass tag (TMT or TMTpro) reagents (Thermo Scientific), pooled ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were labeled for 1 hour at room temperature using 11-plex tandem mass tag (TMT) reagents (Thermo Scientific) resuspended in 40% acetonitrile ...
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bioRxiv - Molecular Biology 2020Quote: ... a sequence containing the same three tags and two stop codons in each frame was cloned into a pcDNA3.1 (Invitrogen) expression vector using EcoRI and Eco0109I restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... which were then separated using SDS-PAGE and visualized using Wester blotting with an anti-his tag antibody (Invitrogen).
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bioRxiv - Biochemistry 2021Quote: ... which were then separated using SDS-PAGE and visualized using Wester blotting with an anti-his tag antibody (Invitrogen).
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bioRxiv - Immunology 2021Quote: ... Dharmacon™) fused in-frame upstream of the stop codon with a V5 epitope tag (Gateway, Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... Empty pGY1 (encoding for no tag) was rendered Gateway-compatible via Gateway™ Vector Conversion System (Thermo Fisher Scientific). To create expression vectors for proteins C- or N-terminally tagged by GFP or mCherry ...
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bioRxiv - Cancer Biology 2022Quote: ... or the serially deleted version of AR was fused in-frame with Flag tag and subsequently cloned into the mammalian expression vector of pcDNA3.1 (Invitrogen). GST fusion constructs with full-length EZH2 or EZH2TAD ...
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bioRxiv - Developmental Biology 2022Quote: Modified versions of pMDC7 carrying the GFP or mCherry tags 61 were used to insert the coding sequence of UBP5 and SWNΔSET via Gateway cloning (Invitrogen). Vectors were transformed in Agrobacterium tumefaciens (Agrobacterium ...
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bioRxiv - Plant Biology 2022Quote: DNA templates of twin-strep-tag (TST) modified D1 and uS2c proteins were obtained from Invitrogen (GeneArt Gene Synthesis). Therefore ...
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bioRxiv - Microbiology 2022Quote: ... with 1.3 µg monoclonal antibody detecting the Twin-Strep-tag and 0.325 µg goat anti-human Alexa Fluor 488 (A11013, Invitrogen). Biotinylated lectins were precomplexed (on ice ...