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Citations for Thermo Fisher :
5201 - 5250 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The ANDV or SNV M gene cDNA was amplified with a SuperScript IV One-Step RT-PCR kit (Thermo Fisher Scientific) using primers flanking the M gene (Forward primer ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen, USA) after serial incubations at 40 °C for 60 min and 90 °C for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA was then incubated with 5M sodium chloride and isopropanol and incubated with Dynabeads M-270 Streptavidin (Thermo Fisher, 65305) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: One µg of DNase-digested RNA was used as input to generate cDNA using the M-MLV reverse transcriptase (ThermoFisher Scientific) and random hexamer primers following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific) in DreamTaq buffer (10 μL total volume ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were stained overnight with the following primary antibodies diluted in blocking buffer: mouse anti-neurofilament-M (1:1500, RMO270, Invitrogen, RRID:AB_2315286); rabbit anti-6xHisTag (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Mass spectrometry experiments were performed using liquid chromatography (LC/MS) using an Acquity M-Class UPLC (Waters) followed by mass spectrometry using an Orbitrap Q Exactive HF-X (Thermo Scientific). For a typical LC/MS experiment ...
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bioRxiv - Plant Biology 2022Quote: ... synthesis of cDNA was performed using an oligo(dT) primer and RevertAid M-MuLV reverse transcriptase (Thermo Fisher Scientific, MA, USA) following manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... Synthesis of cDNA was performed using an oligo(dT) primer and RevertAid M-MuLV reverse transcriptase (Thermo Fisher Scientific, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Total liver cholesterol and triglycerides were determined with colorimetric assay kits (L-Type Triglyceride M – Fujifilm Wako, Infinity Cholesterol Liquid Stable Reagent – Thermo Fisher) and normalized to total protein content.
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bioRxiv - Molecular Biology 2022Quote: ... Approximately 500 ng of peptide sample was injected onto a Waters M-Class nanoAcquity UHPLC system (Waters, Milford, MA) coupled to an ESI Orbitrap mass spectrometer (Q Exactive plus, ThermoFisher Scientific) operated as described in Maitland et al ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was eluted in 50 μl water and precipitated with 0.75 M ammonium-acetate and 10 μg glycogen (ThermoFisher, Cat# R0551) by adding 125 μl ethanol (100 %) ...
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bioRxiv - Microbiology 2022Quote: ... the robot adds 2 volumes of 30% PEG 6000 (in 1.6 M NaCl) and 35ul of 1:5 diluted Glycoblue (Invitrogen, Thermo Fisher) to each well ...
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bioRxiv - Immunology 2022Quote: ... was used to cut 16 μm thick spleen sections or 20 μm thick auricular lymph node sections which were mounted on SuperFrost+ glass slides (Fisher Scientific). Spleen sections were either acetone or PFA fixed ...
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bioRxiv - Bioengineering 2022Quote: ... 20 ng/ml Oncostatin M and 1% (v/v) MEM Non-Essential Amino Acids Solution (Thermo Fisher Scientific, catalogue no. 11140050), 10 uM DAPT for another 10 days with the replacement of media every 48h ...
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bioRxiv - Bioengineering 2022Quote: ... The carotid arteries were then washed with HEPES-PSS and submaximally constricted 10-15% using 10−6 M phenylephrine (207240100; Acros Organics). Vasodilation was measured by treating carotid arteries for five minutes with RBC-EVs isolated using exoEasy ...
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bioRxiv - Microbiology 2023Quote: Experiments were conducted in solution with an initial composition of either 8 g/l (0.14 molal (m)) NaCl solution (ThermoFisher Scientific, 207790010), 5.8 g/l (0.14 m ...
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bioRxiv - Physiology 2023Quote: ... The synthesis of complementary DNA (cDNA) was performed by the reverse transcription (RT) of mRNA using M-MLV reverse transcriptase (Thermo-Fisher Scientific). Quantitative PCR reactions were performed using the StepOnePlus real-time PCR cycler (Thermo-Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was collected and 200 µg were incubated with 5 μl of buffer equilibrated DynabeadsTM M-280 Streptavidin (Invitrogen, 11205D) for 15 min at 4 °C in rotation to perform a pre-clearing step ...
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bioRxiv - Cell Biology 2023Quote: ... MS experiments were performed using liquid chromatography (LC) with an Acquity M-Class UPLC (Waters) followed by MS using an Orbitrap Fusion Tribrid MS (Thermo Scientific). A flow rate of 300 nL/min was used ...
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bioRxiv - Microbiology 2024Quote: ... Polprim and RPA -specific mAbs (approximately 100 µg of each ligand) were separately bound to 5mg of M-280 Tosyl activated Dynabeads (Invitrogen # 14203) O/N at 37⁰C on a rotating wheel ...
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bioRxiv - Molecular Biology 2024Quote: ... was injected onto a Waters M-Class nanoAcquity HPLC system (Waters) coupled to an ESI Orbitrap mass spectrometer (Q Exactive plus, ThermoFisher Scientific) operating in positive mode ...
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bioRxiv - Molecular Biology 2024Quote: ... the chitinous eggshells were first digested with base-bleach treatment (0.4 M KOH, 2% sodium hypochlorite [Fisher Scientific, catalog #SS290-1]) for 1.5 hours at 30°C ...
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bioRxiv - Molecular Biology 2024Quote: ... extracts with high percentages of ssDNA were analyzed on denaturing gels (1 M urea, 0.8% agarose, 1x Tris-acetate-EDTA (TAE) buffer and 1x SybrGold (Thermo Fisher Scientific)) at 50 V for 3 hours ...
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bioRxiv - Microbiology 2024Quote: ... OD600 was adjusted to 1 and 5 μl were mixed with 500 μl of RPMI + 1% CA + 10 μM EDDHA+100 µg holo-transferrin per well (final OD600 of 0.01) in a 48 well microtiter plate (Nunc, Thermo Scientific). Holo-transferrin serves as iron source ...
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bioRxiv - Microbiology 2024Quote: ... auerus::sGFP together with 5 µl of competing strain were mixed with 500 µl of RPMI+1% CA+10 µM EDDHA+100 µg Holo-Transferrin per well (final OD600 of 0.02) in a 48-well microtiter plate (Nunc, Thermo Scientific). OD600 and fluorescence intensity (Ext.480 nm/Em.525 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified antibodies were then neutralized with 14 μl of 1 M Tris-HCl pH 8.0 and buffer exchanged into PBS using Zeba Spin Desalting plates (Thermo Fisher Scientific). Purified antibodies were then stored at 4°C until use.
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bioRxiv - Neuroscience 2024Quote: ... samples were diluted to 1 M urea with solution containing 468 μl of 50 mM ammonium bicarbonate and 7 μg of Trypsin (Thermo Fisher). The samples were subsequently incubated overnight at 25 °C for digestion with trypsin ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated with proper antibodies and visualized by a FluorChem M chemi-luminescent imager (ProteinSimple, San Jose, CA) with enhanced chemiluminescence (Thermo Fisher). The antibodies used in this study are shown in Supplementary Table 1.
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bioRxiv - Molecular Biology 2023Quote: ... in 25 mM sodium citrate pH 5.0 buffer containing 7 M urea and 10.5 mM EDTA with 1 U RNase T1 (ThermoFisher Scientific, Waltham, MA) at 55°C for 5 min ...
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bioRxiv - Genetics 2023Quote: ... Samples were read on a full scan positive mode from 100-600 m/z and data were analyzed using Xcalibur software (Thermo Scientific). All nucleosides were compared to exogenous standards which allowed the interpolation of concentrations of sample nucleosides ...
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bioRxiv - Biochemistry 2024Quote: ... The FeS cluster was removed according to 74 by incubation of the protein with 200 µM H2O2 and 10 mM EDTA for 1 h at room temperature following filtration through a Zeba spin column (Thermo Scientific). The respective activity assays were performed in an anaerobic chamber (Coy Laboratory Products ...
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bioRxiv - Cancer Biology 2024Quote: ... Tris-buffered saline (TBS: 20 mM Tris-HCl, pH 7.5, containing 0.15 M NaCl) with 0.3% Tween 20 (Fisher Scientific, Cat# BP337-100)(TBS-T ...
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bioRxiv - Evolutionary Biology 2024Quote: Fluorescently labeled samples were mounted in glycerol mounting solution (80% glycerol, 0.1 M Tris, pH 8.0) on microscope slides coated with poly-L-lysine (Thermo Fisher Scientific #86010). Samples were imaged at ×40 on a Leica TCS SP8 confocal microscope ...
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bioRxiv - Immunology 2024Quote: Whole intact tumours were harvested on days 10-13 post engraftment and immediately immersed in fixative solution (1% PFA, 75 mM L-lysine [Sigma, Cat. L5501], 10 mM sodium m-periodate [Thermo Scientific Pierce ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed in staining buffer (DPBS+/+ + 2% FBS + 0.4% (v/v) + 0.5 M EDTA (Fisher Scientific; Cat. No. AAJ15694AP)).
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bioRxiv - Immunology 2024Quote: ... Fc/2 portions obtained from native Herceptin were diluted to 0.1 mg/ml and a final concentration of 4 M guanidinium HCl (Thermo Fisher Scientific) supplemented with 25 mM TCEP and were incubated at 60 °C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... followed by staining in 0.5 ml 0.2 M sodium bicarbonate containing 50 mg/ml Alexa Fluor 488 NHS Ester (succinimidyl ester) (Thermo Fisher Scientific #A20100) for 30 min at RT ...
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bioRxiv - Biochemistry 2024Quote: ... 0.15 pmol DNA of 3 kb PCR substrates with a single biotin (GC227 and GC228) were coupled with 5 μl of Dynabeads M-280 streptavidin (Invitrogen 11205D) in 10 μl of binding buffer (5 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... 000 cells were seeded onto a 6-well plate and reversely transfected with 25 nM of either non-targeting control siRNA (Horizon Discovery, D-001206-13-20) or SMARTpool siRNA targeting Znf706 (Horizon Discovery, M-021025-01-0005) using Lipofectamine RNAiMAX (Invitrogen, 13778150). The transfection media was exchanged after 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01, in 10□×□PBS pH 7.2 (Gibco, #70013032). The final volume was topped up with DMEM/F12 (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed with 0.1 M phosphate buffer and incubated with Alexa Fluor secondary antibodies (Fisher Scientific/Invitrogen, dilutions 1:1000) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed with 0.1 M phosphate buffer and incubated with Alexa Fluor secondary antibodies (Fisher Scientific/Invitrogen, dilutions 1:1000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... PBS was replaced with a 1:6 (weight: volume) solution of 0.5 M acetic acid (984303; Thermo Fisher, Waltham, MA, USA) with 1 mg/ml pepsin (20343 ...
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bioRxiv - Biophysics 2023Quote: ... 200 µL of 10 µM P (0.25 µM FITC-P) in reference condition was loaded into a Nunc Lab-Tek Chambered Coverglass (ThermoFisher Scientific Inc) and incubated for 2 h at 22°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately 500 ng of peptide sample was injected onto a Waters M-Class nanoAcquity UHPLC system (Waters, Milford, MA) coupled to an ESI Orbitrap mass spectrometer (Q Exactive plus, ThermoFisher Scientific). Samples were trapped for 5 minutes at a flow rate of 5 μL/min using 99% buffer A and 1% buffer B on a Symmetry BEH C18 Trapping Column (5 mm ...
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bioRxiv - Molecular Biology 2023Quote: ... as determined by BCA assay was then injected onto a Waters M-Class nanoAcquity HPLC system (Waters) coupled to an ESI Orbitrap mass spectrometer (Q Exactive plus, ThermoFisher Scientific). Buffer A consisted of mass spectrometry grade water with 0.1% FA and buffer B consisted of acetonitrile with 0.1% FA (ThermoFisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The mass spectra of 50–650 m/z were recorded on an ISQ™ Series Single Quadrupole MS (Thermo Scientific™) using EI ionization mode and a scan time of 0.204 s ...
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bioRxiv - Cell Biology 2023Quote: ... 0.02 M Hepes buffer 1M in 0.85 % NaCl (Cambrex no 0750, #BE17-737G) and 10 ml 1X Glutamax (100X, Gibco #35050-038), 5 ml in 500 ml medium ...
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bioRxiv - Bioengineering 2023Quote: ... The suspension was prepared by adding 40 μm diameter polystyrene beads (Fluoro-Max Green 35-7B, Thermo-Fisher) to 50 mL of 1X PBS (Gibco PBS, Fisher Scientific) to achieve a final concentration of approximately 30 beads/mL.