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Citations for Cell Signaling Technology :
8301 - 8350 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... after which the sections were incubated overnight at 4°C with rabbit anti-cFos antibody (1:6400 dilution, #2250, Cell Signaling Technology, Danvers, MA, USA), 1% NHS ...
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bioRxiv - Cancer Biology 2023Quote: ... The HRP-conjugated secondary antibodies (anti-rabbit and anti-mouse antibodies, respectively ref 7074 and 7076) were supplied by Cell Signaling Technologies (Danvers, Massachussets, USA).
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bioRxiv - Cancer Biology 2023Quote: ... were used as primary antibodies and were detected with anti-rabbit or anti mous IgG HRP-conjugated secondary antibodies (Cell Signaling, ref #7074 and #7076). Proteins were detected by chemiluminescence using Immobilon Forte Western HRP substrate (Merk Rahway ...
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bioRxiv - Cancer Biology 2023Quote: ... and secondary horseradish peroxidase (HRP)-conjugated antibodies goat anti-rabbit IgG (#7074) and horse anti-mouse IgG (#7076) were purchased from Cell Signaling Technology (Danvers, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Oligodendrocyte differentiation was assessed by incubating free-floating sections overnight at room temperature with rabbit PDGFRα (D1E1E) XP® antibodies (Cell Signaling, cat #3174,1:200), mouse anti-CC1 (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... membranes were washed three times in TBST for 5 minutes and incubated for 1 hour with horseradish peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology Cat# 7074, RRID:AB_2099233) or anti-mouse IgG (Cell Signaling Technology Cat# 7076 ...
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bioRxiv - Plant Biology 2023Quote: ... MAPK activation was detected by western blot with Phospho-p44/42 MAPK (Erk1/2; Thr-202/Tyr-204) rabbit monoclonal antibodies #9101 (Cell Signaling Technology, Danvers, MA, USA). Blots were stained with PageBlue Protein Staining Solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... used at 1:500 (western blot) and 1:50 (immunofluorescence) and rabbit anti–phospho-MLC2 raised against phospho-Thr18/Ser19 (Cell Signaling Technology; catalog no. 3674) used at 1:500 (western blot ...
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bioRxiv - Cancer Biology 2023Quote: ... The intestines were dissected and fixed in 4% paraformaldehyde for 1 h before blocking with 5% NGS and staining with rabbit-PH3 primary antibody (Cell Signaling Technology, Leiden, Netherlands, #3377) overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... The membrane was then washed three times in the wash buffer for five minutes each and incubated for one hour at room temperature with agitation in an anti-rabbit HRP-conjugated secondary antibody (Cell Signaling Technology, 7074V, 1:1000) diluted in the wash buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualised using the following primary and secondary antibody combinations: Cyclin B1 was detected with a monoclonal rabbit antibody (Cell Signaling Technology, 12231S, 1:800) and visualised using a goat anti-rabbit Alexa Fluor 647 secondary antibody (Molecular Probes ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies (mouse, anti-claudin-4, ThermoFisher, cat: 32-9400, at 1:200 dilution; rabbit, anti-GM130, Cell signaling, cat: 12480, at 1:1600 dilution) were incubated (BSA at 2% ...
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bioRxiv - Cancer Biology 2024Quote: ... sonicated chromatin was supplemented to final concentration of 1.1% Triton-X and 5% glycerol and subsequently set up for immunoprecipitation with the following antibodies: Rabbit Anti-POU2F3 (Cell Signaling E5N2D, #36135, 1:50), mouse anti-Rpb1 (Cell Signaling 4H8 ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed three times with TBST for 5 minutes and immunoblotted with the appropriate HRP-conjugated secondary antibodies: anti-rabbit (Cell Signaling, Cat#7074, 1:2000) or anti-mouse (Cell Signaling ...
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bioRxiv - Cell Biology 2024Quote: Antibodies against pAKT (Ser473) pAKT (Thr308) and AKT (pan) (all rabbit polyclonal) and antibody against β-actin was from Cell Signaling Technology (Danvers, MA, USA). Antibody against fibronectin (ab6328 ...
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bioRxiv - Physiology 2023Quote: ... 5% low-fat dry milk (blocking solution) and incubated overnight at 4°C with a rabbit monoclonal anti-Numb antibody (#2756, Cell Signaling Technology, Inc., Danvers, MA) at 1:1000 dilution in 5% BSA in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... 4 serial brain sections from each animal were stained with rabbit anti-human Aβ antibody (clone D12B2; Cell Signaling Technology Europe, Frankfurt am Main, Germany) and Cy3-conjuagted goat anti-rabbit IgG (Jackson ImmunoResearch Europe Ltd ...
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bioRxiv - Biophysics 2023Quote: ... Detection was performed with the UVP ChemStudio Imaging System (Analytik Jena, Jena, Germany) using horseradish peroxidase-linked anti-mouse (#38) and anti-rabbit (#29) secondary antibodies (Cell Signaling Technology, Danvers, MA, USA), and the ECL western blotting reagent ...
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bioRxiv - Cell Biology 2024Quote: ... The sections were treated with the following primary antibodies (αSMA, mouse, Cat. No. ab7817, Abcam at 1:300 dilution, PTEN rabbit, Cat. No. D8H1X, Cell Signaling at 1:300 dilution) sequentially over 2 days with 2 overnight incubations with the primary antibody in blocking buffer (dilution 1:200 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Membranes were washed 3 x and incubated for 1 h at room temperature with the horseradish peroxidase (HRP)-conjugated secondary antibodies (goat anti-rabbit or goat anti-mouse-HRP, 1:2000; Cell Signaling Technology, Danvers, MA, USA), washed in LSWB and incubated with chemiluminescent HRP substrate reagent (Super Signal West Femto ...
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bioRxiv - Microbiology 2024Quote: ... and cell extracts were incubated for 3 h at 4°C with beads pre-coupled overnight with rabbit anti-LC3C (Cell Signaling Technology, 14736, 1:250) antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were incubated with secondary antibodies (goat anti-rabbit monoclonal IgG, HRP-linked, 1:1000, Cell Signaling Technology, #7074S; horse anti-mouse IgG monoclonal, HRP-linked, 1:2000, Cell Signaling Technology, #7076S) diluted in blocking solution for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... mouse anti-GM130 (BD transduction Laboratories, ref:610822, 1:1000, rabbit ITGB1, ITGB4, ITGA4 (from integrin Ab Sampler kit, Cell signaling ref: 4749, 1:1000), rabbit anti-EGFR (Cell Signaling ref ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were washed with TBST for 10 min three times before incubation with secondary antibodies [Anti-rabbit IgG-HRP (Cell Signaling Technology 7074S, 1:5000), Anti-mouse IgG- HRP (Cell Signaling Technology 7076S ...
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bioRxiv - Bioengineering 2024Quote: ... The membrane was washed with TBST and incubated in TBST + 5% (w/v) nonfat milk and anti-rabbit IgG HRP-linked antibody (Cell Signaling catalog #7074, 1:1000) for 1 hour then washed in TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then blocked with 5% goat serum/2.5% BSA for at least 1 hour before overnight incubation at 4°C with an antibody cocktail consisting of rabbit anti-vimentin (1:100) (catalog #: 5741; Cell Signaling Technologies; Danvers, MA, USA), mouse anti-dystrophin IgG2b (1:50 ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit monoclonal antibodies against TRIM46 (#1 in text: Abcam Cat# ab307967, RRID:AB_3096363, 1:500, #2 in text: Cell Signaling Technology Cat# 92574, RRID:AB_3096364, 1:1000), guinea pig monoclonal antibody against TRIM46 (Synaptic Systems Cat# 377 308 ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 4-times for 10 min in 1 x PBS-T before 1 h incubation at room temperature with secondary anti-rabbit IgG coupled to horseradish peroxidase (Cell Signaling Technology, Danvers,MA, USA) diluted 1:1,000 in 1 x PBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... slices were moved to the primary antibody solution containing one or more of the following antibodies in blocking solution and were left there overnight: rabbit-anti-c-Fos (1:1000; Cell Signaling Technology, 2250s, Leiden, Netherlands), chicken-anti-GFP(1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with a FACS buffer (FACS solution supplemented with 4% fetal calf serum) and incubated with anti-rabbit secondary antibody Alexa Fluor 488™ (#4412, Cell Signaling Technology, Danvers, MA, USA)(1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... we used identical procedures except that we used LV-Syn-DIO-mTagBFP2-2A-GluN2B (1 × 109 TU/ml titer, 2 μL) and GluN2B antibody (#4207S, Cell Signaling, 1:1000, rabbit polyclonal antibody) (Supplementary Fig ...
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bioRxiv - Physiology 2020Quote: ... Membranes were washed and incubated with HRP-conjugated secondary antibodies at 1:10,000 (Mouse, Cell Signaling technology Cat# 7076, RRID AB_330924; Rabbit, Cell Signaling technology Cat# 7074, RRID AB_2099233) for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... slices were incubated overnight at 4 °C with the respective primary antibodies (rat-monoclonal anti- BrdU −1:200- Abcam; Rabbit polyclonal antibody anti-DCX,1:500- Cell Signaling; mouse monoclonal anti-NeuN- Millipore) diluted in a 0.1 % BSA and 0.125 % Triton X-100 solution ...
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bioRxiv - Neuroscience 2020Quote: ... PFA- or NBF-fixed sections were incubated with the following primary antibodies: rabbit anti-cleaved caspase 3-D175 (Cell Signaling, #9661, 1:100, PFA-fixed tissue), Ctip2 (Abcam ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated with TBS and 3% horse serum and 0.25% Triton X-100 before being incubated with Alexa Fluor© 488 Anti-Rabbit IgG (Cell Signaling, cat#4412S, diluted 1:250) for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... at 1:100 in 3% Casein in 1X Tris Buffered Saline with TWEEN ® 20 (Sigma-Aldrich, Oakville, Ontario, and Anti-rabbit HRP-linked Antibody (7074S - Cell Signaling Technology, Danvers, MA) at 1:2000 ...
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bioRxiv - Microbiology 2022Quote: ... the cells were washed twice with the blocking buffer and incubated at 4 °C with primary antibodies (mouse anti-Strep, Qiagen, Cat #: 34850, 1: 150 dilution) (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated at room temperature with HRP-conjugated secondary antibodies ((goat anti-rabbit HRP conjugated, Cell Signaling, Cat #: 7074, 1:5,000 dilution) (goat anti-mouse HRP conjugated, Cell Signaling, Cat #: 7076, 1:5,000 dilution) prepared in the WB blocking buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... #2639), anti-GAPDH (clone 14C10, #2118), rabbit anti-HA-tag (clone C29F4, #3724) antibodies for immunoblots were purchased from Cell Signaling Technology (Frankfurt am Main, Germany). Mouse anti-β-actin (clone AC-15 ...
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bioRxiv - Neuroscience 2022Quote: ... NF-KB (1:1000 mouse anti-total NF-κB; Cell Signaling Technology #6956; RRID #AB_10828935 or 1:1000 rabbit anti-phospho NF-κB-S536; Cell Signaling Technology #3033; RRID #AB_331284); Akt (1:250 mouse anti-total Akt ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8 (ab4055), CD4 (EPR6855, ab133616) at a 1:500 dilution and PD-L1 (rabbit monoclonal anit-PD-L1 primary antibody; Cell Signaling Technology, clone: E1L3N, CAT#13684) at a 1:200 dilution on the Leica BOND RX ...
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bioRxiv - Plant Biology 2023Quote: ... Hemagglutinin (HA)-tagged mutant PpRopGAP1 in yeasts was detected with an anti-HA primary antibody (C29F4) of rabbit origin (Cell Signaling Technology, cat. 3724T, 5000× dilution) and an anti-rabbit-HRP (goat ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were then reacted with a secondary antibody (Anti-mouse IgG, and Anti-rabbit IgG conjugated to Horseradish Peroxidase (both from Cell signaling, Cat # 7076 and # 7074, respectively), developed using Western BrightTM enhanced chemiluminescence reagent (Advansta) ...
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bioRxiv - Developmental Biology 2023Quote: ... diluted 1:100), Rat anti-GFP monoclonal antibody (nacalai tesque, GF090R, diluted 1:1000) and rabbit anti-pSmad1/5/9 antibody (Cell Signaling Technology, #13820, diluted 1:100). After the primary antibody incubation ...
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bioRxiv - Bioengineering 2023Quote: ... The membranes were blocked with 5 % dry skimmed milk in Tris-buffered saline containing 0.05 % (v/v) Tween-20 (TBST) for 1 h and incubated with rabbit anti-phospho Zap-70(Tyr319)/ Syk (Tyr352) (Cell Signaling Technology, Cat # 2717T, Clone 65E4), anti-β actin antibody (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were incubated in the following primary antibodies for 3 hours at room temperature: phospho-S6 Ribosomal Protein Ser240/244 (rabbit monoclonal, 1:1000 dilution, Cell Signaling Technology, catalog #5364, RRID: AB_10694233), phospho-AKT Ser473 (rabbit monoclonal ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were analyzed by western blot using a mouse anti-HA antibody (Covance, Cat #MMS-101P; Sorcs1HA/HA KI) or a rabbit anti-HA antibody (Cell Signaling Technology, Cat #3724; Nrxn1αHA/HA KI). A total protein stain (Ponceau ...
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bioRxiv - Genetics 2019Quote: ... the membranes were incubated for 12 hours or longer at 4°C with rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 1:1000 dilution) or mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were blocked with 5% skim milk in TBST for 1 h at room temperature and incubated with anti-human p53 antibody (Cell Signaling Technologies, #9282, rabbit polyclonal, 1:1000) in TBST containing 5% skim milk (4°C ...
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bioRxiv - Cell Biology 2021Quote: ... peroxidase and haematoxylin staining were performed on deparaffinised sections that were sequentially incubated with rabbit anti-insulin antibody (Cell Signaling Technology C27C9, Danvers, MA, USA, 1:400), then with F(ab’)2 conjugates with Biotin-SP (Jackson ImmunoResearch 711-066-152 ...