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Citations for Cell Signaling Technology :
8201 - 8250 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated with the rabbit monoclonal anti-FosB primary antibody (dilution: 1:1000, product number: 2251S, Cell Signaling Technology, Danver, MA, USA) at 4 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated with a 1:500 dilution of Alexa Fluor® 488-labeled rabbit anti-His tag antibody (Cell Signaling Technology, Danvers, MA) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... PFA-fixed sections were incubated with the following primary antibodies overnight at 4°C: rabbit anti-cleaved caspase 3 (Cell signaling, #9661, 1:100), mouse anti-BrdU (Dako ...
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bioRxiv - Cancer Biology 2022Quote: ... was from BioBharati LifeScience (Kolkata, India). ERα (D8H8) antibody (Cat No. 8644S) and anti-rabbit secondary antibody (Cat. No. 7074S) were from Cell Signaling Technology (MA, USA). 17β-estradiol (E2 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with blocking solution for 90min then with a combination of two primary antibodies: rabbit anti-Fos (1:2000; Cell Signaling Technology, RRID #2250) and chicken anti-mCherry (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... The membrane was then washed 3 times with TBST and the secondary goat anti-rabbit IgG horseradish peroxidase (HRP)-conjugated antibody (1:5000; cat. no. 7074, Cell Signaling Technology, The Netherlands) was added and allowed to bind at room temperature for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... Immunoprecipitation was performed overnight at 4°C using 300µg of chromatin per reaction and polyclonal rabbit anti-Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad8 (Ser426/428) (Cell Signaling, #9511; 1:1000). Pulldown of the immunoprecipitated chromatin using protein-G sepharose beads and elution were performed as described (Schwaiger et al. ...
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bioRxiv - Neuroscience 2021Quote: ... gift from Carmen Birchmeier and Thomas Müller of the Max Delbrück Center for Molecular Medicine in Berlin) and rabbit anti-cleaved Caspase-3 (1:1000, Cell Signaling Technology Cat# 9661, RRID:AB_2341188). SCs were then washed in PBS and incubated for 2 h at RT with secondary fluorescent antibodies (goat anti-rabbit-conjugated 649 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies were diluted with blocking solution and incubated with cells overnight at 4°C: Cleaved caspase-3 (rabbit, Cell Signaling #9661, 1:500), Anti-Nestin (mouse ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was washed three times with Tris-buffered saline containing 0.01% Tween 20 and incubated for one hour with secondary rabbit (Cell Signaling Technology, Inc, Danvers, Massachusetts) or goat anti-mouse HRP-conjugated antibody (Abcam Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... Monoclonal mouse anti-human Ki67 was from Dako Agilent Technologies (Les Ulis, France) and rabbit polyclonal anti-cleaved caspase 3 antibody was provided by Cell Signaling Technology (Ozyme, Saint Cyr l’ecole, France). All Alexa-conjugated secondary antibodies were purchased from Thermo Fisher Scientific.
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bioRxiv - Cancer Biology 2021Quote: ... The amplification time was 100 minutes and the primary antibodies pair to detect the interaction was rabbit anti-Aurora-A (14475, Cell Signaling, 1:100 o.n.) and mouse anti-N-Myc (NCM II 100 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then rinsed and blocked with 5% BSA for 1hr prior to overnight incubation (4°C) with monoclonal rabbit anti-Phospho-p38 MAPK (Thr180/Tyr182) (D3F9)-XP (Cell signaling, 4511T, 1:1,000) or monoclonal rabbit anti-p38 MAPK (D13E1 ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-CEP135, (homemade, raised against a.a. 649-1140, rabbit anti-acetylated-α-Tubulin (1:1,000 IF, 1:500 IHC; Cell Signaling Technologies, cat. No. 5335), rabbit anti-CNTROB (1:1,000 ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were washed for 15 min in TBST four times then incubated for 2 hours at room temperature with an anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling #7074; 1:20,000). Blots were imaged and quantified using ImageQuant (Amersham).
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bioRxiv - Cancer Biology 2019Quote: ... Reactions were analyzed by NU-PAGE Nu-PAGE and immunoblotted with the rabbit monoclonal antibody to CD82 (D7G6H) was used (Cell Signaling Technology Cat# 12439S).
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bioRxiv - Developmental Biology 2019Quote: ... Blots were probed overnight at 4 deg C with rabbit anti-V5 (Cell Signaling Technologies; 1:1000 in 5% BSA, 0.1% Tween 20, TBS) or for 1 hr at room temperature with mouse anti-β actin (GeneTex ...
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bioRxiv - Physiology 2020Quote: ... The blots were washed and immunoreactive bands were detected by incubation with anti-rabbit HRP conjugated antibody (dilution 1:2000 in blocking solution; Cell signaling, Cat no. 7074S) for 1h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were incubated at 4°C overnight with the following mouse-specific primary antibodies: anti-NLRP3 (D4D8T, rabbit monoclonal antibody, 1:1000, #15101, Cell Signaling Technology, Danvers, MA), anti-cleaved Caspase 1 (p20 ...
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bioRxiv - Physiology 2021Quote: ... the membranes were washed 3 times in TBS-T for 5 minutes and incubated for 1 hour in HRP-conjugated anti-rabbit antibodies (Cell Signaling Technology, London, UK) at dilution of 1:5000-1:10,000 ...
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IL-27R signaling serves as immunological checkpoint for NK cells to promote hepatocellular carcinomabioRxiv - Immunology 2020Quote: ... Each membrane was washed and primary antibodies (except β-actin-HRP) were detected with a 1:5000 dilution of HRP-conjugated rabbit anti-mouse IgG (Cell Signaling Technology, 7076S) or mouse anti-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... the membrane was incubated with HRP-conjugated anti-rabbit IgG or HRP-conjugated anti-rat IgG secondary antibody (Cell Signaling Technology, Danvers, MA, USA) for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were stained with Ponceau S solution and blocked with 5% nonfat milk in PBS/0.05% Tween 20 for 1 h at RT and subsequently incubated at 4 °C overnight with the following primary antibodies: rabbit anti-MBP (1:1000; Cell Signaling, Danvers, MA, USA), mouse anti-CRYAB (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit monoclonal, 1:1000), haem oxygenase-1 (HO-1; rabbit polyclonal, 1:1000) or IκBα (mouse monoclonal, 1:1000; Cell Signaling Technology, Leiden, The Netherlands) in Tris-buffer saline solution containing 0.1% Tween-20 and 5% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed with horseradish peroxidase-conjugated antibodies directed against rabbit or mouse IgG (HRP-linked α-mouse IgG (Cell Signaling Technology, Cat. 7076), HRP-linked α-rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Microbiology 2022Quote: ... guinea pig polyclonal NSP3 (Lot 55068, 1:2000), VP6 (Lot 53963, 1:2000) antisera or rabbit monoclonal β-actin (8457S, Cell Signaling Technology (CST), 1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Phosphorylation of Mpk1 and Fmk1 MAPKs was detected using rabbit anti-Phospho-p44/42 MAPK (Erk1/2) antibody (Thr202/Tyr204, #4370; Cell Signaling Technology, Danvers, MA). Phosphorylation of Hog1 MAPK was detected using rabbit anti-Phospho-p38 MAPK antibody ((Thr180/Tyr182 ...
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bioRxiv - Neuroscience 2022Quote: ... blotted with mouse anti-ERK1/2 (p44/42 MAPK) and rabbit anti-phospho-ERK1/2 (p44/42 MAPK) (Cell Signaling Technologies 4696S and 4370S), both at 1:1000 in 2% BSA at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... 0.15%) and respectively incubated overnight with primary and corresponding secondary antibodies [rabbit anti p-mTOR (1:1000; #5536, Cell Signaling Technology, Danvers, MA, USA); rabbit anti mTOR (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Slides were then incubated overnight with the following primary antibodies at 4°C: rabbit anti-Ki67 (1/200; 12202; Cell Signaling Technology, Danvers, MA), rabbit anti-Olfm4 (1/400 ...
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bioRxiv - Immunology 2024Quote: ... anti-Actin and HRP- labeled goat-anti-rabbit or anti-mouse IgGs for Western blot assay were purchased from Cell Signaling Technology (Danvers, MA). Phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Cell Biology 2023Quote: ... The MEL cells were bound to Concanavalin A Beads and Incubated with 1:50 rabbit polyclonal Phospho-Rpb1 CTD Ser5 and Ser2 antibody (Cell Signaling 13523 and 13499). Guinea pig a-rabbit antibody was used at 1:100 dilution as secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... secondary antibody staining was performed for 10 minutes at 4°C with Anti-Rabbit IgG conjugated to Alexa Fluor® 488 fluorescent dye (Cell Signaling Technology, 4412S). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed in 1xPBS and incubated with anti-rabbit IgG (H+L) F(ab’)2 fragment conjugated to Alexa Fluor® 488 (4412S; 1:500; Cell Signaling Technology, USA) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated overnight at 4°C in the following primary antibodies: rabbit anti-cleaved NOTCH1 (Val 1744) at 1:1000 (Cell signaling technology, Cat #: 4147S), rabbit anti-NOTCH1 (D1E11 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074) or goat anti-mouse IgG (1:3,000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and the samples were incubated with the following primary antibodies overnight at 4°C: Rabbit anti-p44/42 MAPK (Erk1/2) (1:1000, 4695, Cell Signaling Technology, MA, USA), rabbit anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The membrane was washed four times in 0.1% TBST buffer and incubated for 1 h at room temperature with anti-rabbit IgG conjugated to HRP-linked antibody #7074 (Cell Signaling Technology, Danvers, MA) diluted 1:5,000 with 0.05% TBST containing 5% skimmed milk ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated with horseradish peroxidase-conjugated secondary antibodies (anti-rabbit IgG at 1:5000 dilution or anti-mouse IgG at 1:5000 dilution; both from Cell Signaling Technology, Danvers, MA), and the bound proteins were visualized using a chemiluminescence-based detection kit (ImmunoStar Zeta ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were washed three times with 0.5% BSA–PBS and incubated with a horseradish peroxidase-conjugated goat anti-rabbit antibody (30 ng/ml) (Cell Signaling Technology, Danvers, MA, USA) in 1% BSA–PBS for 1 h at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... and incubated for 1h at RT with peroxidase-conjugated secondary antibodies (anti-mouse IgG, Cell Signaling, cat. 7076; anti-rabbit IgG, Cell Signaling, cat. 7074) diluted 1:5000 in 5% non-fat dried milk in TBS-T solution ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, Catalog # 7074) or goat anti-mouse IgG (1:3000 ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed with TBST three times and then incubated at room temperature for 60 min with secondary antibody goat anti-rabbit IgG (1:3000, Cell Signaling Technology, catalog # 7074). Then the membranes were washed with TBST four times ...
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bioRxiv - Biochemistry 2023Quote: ... antibodies and the secondary anti-rabbit horseradish peroxidase-linked IgG antibody (NA944V, GE Healthcare for western blotting and 7074S Cell signaling Technology for IHC) were purchased from the indicated vendors ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:4700 in 5% non-fat milk (anti-rabbit IgG #ADI-SAB-300-J, Enzo Lifesciences, Farmingdale NY or anti-mouse IgG #7076, Cell Signaling Technology, Danvers MA). The membranes were imaged using Azure Biosystems Radiance Plus substrate solution and an Azure c600 Imaging System.
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bioRxiv - Biochemistry 2023Quote: ... Coverslips were then blocked with 5% BSA for 60 minutes at room temperature and incubated overnight at 4°C with primary antibody γH2AX (rabbit monoclonal; Cell Signaling 9718T, 1:400). Texas red species-specific secondary antibodies (Jackson ImmunoResearch Laboratories Inc. ...
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bioRxiv - Genomics 2023Quote: ... The blots were washed with 1X TBST (6 washes of 5 min each) followed by incubation with HRP-conjugated anti-rabbit secondary antibody (Cat. No. 7074S, Cell Signaling Technology, MA, USA) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... CUT&RUN was performed from 500,000 cells per condition in triplicates as previously described in (Skene et al, 2018) using the following antibodies: rabbit anti-HA (Cell Signaling Technology, Cat# 3724); rabbit anti-MLL1 (Epicypher ...
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bioRxiv - Neuroscience 2023Quote: ... for 1h at RT and then immunolabeled with rabbit primary antibodies, anti-GIRK2 (1:500, Alomone labs, APC-006, RRID: AB_2040115) and anti-actin (1:2000, Cell Signaling Technology, 4970S, RRID: AB_2223172) overnight at 4°C in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The FGFR1-probed membranes were washed 5x for 5min in TBST and then probed with anti-rabbit HRP-conjugated antibody (7074S, Cell Signaling, Danvers, MA, USA) for 1h at RT ...