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Citations for Cell Signaling Technology :
8001 - 8050 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... with HRP-conjugated secondary antibodies (Goat Anti-Rabbit Immunoglobulins/HRP, Dako P0448; Horse Anti-Mouse IgG, HRP-linked, Cell Signaling Technology #7076). BCIP/NBT substrate was used to detect ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed in TBST and then incubated for 45 min at room temperature in blocking buffer containing either anti-rabbit or anti-mouse HRP-linked secondary antibody (Cell Signaling Technology). Membranes were washed in TBST and imaged on the ChemiDoc Imaging System using SuperSignal chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Genetics 2022Quote: ... followed by 1 h incubation in horseradish peroxidase–conjugated secondary antibody (anti-rabbit or anti-mouse, #7074 and #7076, Cell Signaling Technology) diluted in PBS-T 5% milk at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The pSrc/Src ratio was assessed with a rabbit Phospho-Src Family (Tyr416) Antibody (#2101, Cell Signaling Technology, Danvers, MA, USA; RRID:AB_331697), recognizing the phosphorylation at tyrosine 424 in murine Src ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated overnight at 4°C with various antibodies: rabbit anti-pan ubiquitin (1:1,000, #3933S; Cell Signaling Technology, Danvers, MA), rabbit anti-K48-linkage specific polyUb (D9D5 ...
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A balance between pro-inflammatory and pro-reparative macrophages is observed in regenerative D-MAPSbioRxiv - Bioengineering 2023Quote: ... These slides were then stained with rabbit primary antibody (anti-mouse CD11b Antibody from Novus Biologicals or anti-mouse CD11c Antibody from Cell signaling) at 4°C overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Ribosomal protein S6 phosphorylation was detected by a specific rabbit monoclonal antibody against Ser235/236 of the human protein (at 1:5,000; Cell Signaling, Cat#: 4858), followed by a secondary antibody (at 1:5,000 ...
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bioRxiv - Neuroscience 2023Quote: ... immunofluorescence was performed by overnight incubation at 4 °C with a rabbit anti-HA primary antibody (Cell Signaling, #3724, 1:500 dilution), in combination with one of four primary guinea pig antibodies against synaptic markers ...
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bioRxiv - Genomics 2023Quote: ... The membrane was washed five times with TBST and then incubated for 1 h with secondary goat-anti-rabbit-HRP conjugate (1:4000, Cell Signaling Technology) in TBST with 5% non-fat powdered milk ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used in this study: rabbit anti-cleaved Dcp-1 (Asp215) (1:250, Cell Signaling, Danvers, MA, USA), rabbit anti-peroxidasin (Pxn ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were blocked using Intercept Blocking Buffer and incubated overnight in the following primary antibodies: rabbit GAPDH (2118S) from Cell Signaling Technology; rabbit BLOC1S1 (19687-1-AP ...
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bioRxiv - Neuroscience 2023Quote: ... TBR2+ cells were determined in comparison of each cell population measured against immunoreactivity of a Rabbit IgG isotype control (Cell Signaling, #5472). For all measures ...
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bioRxiv - Neuroscience 2023Quote: ... Corresponding secondary antibodies conjugated with horseradish peroxidase (cat no: 7074 Rabbit, cat no: 7076 Mouse, cat no: 7077 Rat, Cell Signaling Technology) in combination with enhanced chemiluminescence reagent (cat no ...
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bioRxiv - Cell Biology 2023Quote: ... before incubating overnight at 4°C at 1:100 in blocking solution for all primary antibodies used: rabbit anti-E-Cadherin (24E10 – Cell Signaling 3195), rabbit anti-Piezo1 (Novus NBP-78537) ...
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bioRxiv - Pathology 2023Quote: ... They were then incubated first with blocking solution for 1 hr and then with a rabbit polyclonal anti-RelA antibody (8242; Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were then incubated with the appropriate secondary HRP-linked antibody as follows: anti rabbit-HRP (1:3000, Cell Signaling Technology 7074) for NEMO ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed in TBS-BSA and incubated overnight on a shaker in TBS-BSA containing Rabbit anti-cFos (1:2,000; Cell Signaling Product# 2250).
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bioRxiv - Cell Biology 2023Quote: ... and stained 1h/RT or overnight/4°C with following primary antibodies in 0,2% Triton-X100 in PBS: PAX6 (rabbit, CS60433, Cell Signaling, 1:300), ZO-1-1A12 (mouse ...
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bioRxiv - Cancer Biology 2023Quote: ... incubation for 1–3 hr at RT with 1:5000 secondary antibody (Cell Signaling Technology goat HRP-linked Anti rabbit IgG #7074), and 3 more 5 min washes with TBST ...
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bioRxiv - Cell Biology 2023Quote: ... and treated for 1.5 h with primary antibody diluted in blocking reagent (NF-κB p65/RELA, Rabbit monoclonal antibody, 1:200, #8242, Cell Signaling Technology). Cells were washed three times in PBS and incubated for 0.5 h with fluorescent-labeled secondary antibody in blocking solution (1:500 ...
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bioRxiv - Biochemistry 2023Quote: ... for ZIP4 or an HRP-conjugated anti-rabbit immunoglobulin-G for β-actin at 1:5000 dilution (Cell Signaling Technology, Catalog# 7074S) by chemiluminescence (VWR) ...
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bioRxiv - Neuroscience 2023Quote: ... were from Thermo Fisher Scientific (Waltham, MA). Rabbit monoclonal antibody to Flotillin-1 (cat. 3253) and Alix (cat. 2171) were from Cell Signaling Technology (Danvers, MA). Mouse monoclonal antibody to CD31 (cat ...
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bioRxiv - Biochemistry 2023Quote: ... Protein lysates were separated by SDS-PAGE and proteins of interests detected with the following antibodies: phosphorylated (p-VASP) Ser239 (rabbit, 1:1000, Cell Signaling #3114S), glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Genetics 2023Quote: ... The following antibodies and reagents were purchased and used in this research: Rabbit anti-cleaved Drosophila Dcp-1 (Asp216) antibody from Cell Signaling Technology, Inc ...
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bioRxiv - Developmental Biology 2023Quote: ... Remove buffer and replace with 100μL DBE supplemented with primary antibody of interest (data presented here: rabbit anti-H3K27Me3 antibody (Cell Signaling, 1:100). Incubate cells plus antibody angled sideways on orbital shaker ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibody incubation was followed by incubation in HRP-conjugated secondary antibody for 1h RT at room temperature (goat anti mouse IgG and goat anti-rabbit IgG, Cell Signaling Technology). Bands were detected by chemiluminescence using ECL solution (WesternBright Sirius ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were blocked in 0.5% BR (Blocking Reagent (Akoya Biociences))/TNT (TN, 0.05% Tween) for 1h at RT and incubated with a primary antibody against pSMAD1,5,9 (Cell Signaling 13820S, rabbit, 1:300) overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... The blots were then incubated with a peroxidase-conjugated anti-rabbit or anti-mouse IgG antibody (1:2000) (# 7074 or #7076, respectively; Cell Signaling Technology). The antibody–antigen reactions were detected using the Clarity™ or Clarity™ MaxWestern Blotting ECL Substrates (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: ... The blots were then washed and incubated for 1 h in goat anti-rabbit HRP conjugated secondary antibody (Cell Signaling Technology, 7074) diluted 1:3000 in TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... These steps were followed by the incubation with primary antibodies overnight at 4°C in a solution of 2% NGS in PBS 1x: chicken anti-mCherry (1:1000, HenBiotech, Portugal) and rabbit anti-NeuN (1:100, Cell Signaling, USA), rabbit anti-Iba1 (1:750 ...
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bioRxiv - Cancer Biology 2023Quote: ... probed with primary antibodies (Supplementary Table 3) and detected with horseradish peroxidase-conjugated α-rabbit or α-mouse secondary antibodies (Cell Signaling). The blots were imaged using a ChemiDoc MP Imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were washed several times with TBST and then incubated with peroxidase-labeled goat anti-rabbit IgG (1:10,000; Cell Signaling-Beverly, Massachusetts). Bands were visualized with SuperSignal West Dura substrate (Pierce Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... Double labeling for phospho-c-Jun and TH was performed with phospho-specific rabbit monoclonal antibody (Cell Signaling, cat.# 3270; 1:500) for c-Jun and sheep anti-TH antibody (Novex Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... c-Jun phosphorylated at the JNK phosphorylation site Ser73 was detected with phospho-specific rabbit antibody (Cell Signaling, cat.# 3270; 1:1,000) and the total c-Jun – with mouse monoclonal antibody (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... in PBST and then incubated for 15 hrs at 4 °C in primary anti-c-Fos (rabbit monoclonal, 1:2000, Cell Signaling 2250) diluted in PBST containing 4% NGS ...
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bioRxiv - Neuroscience 2023Quote: ... anti-Phospho-p70 ribosomal protein S6 kinase (Thr389) (108D2) (pS6K) (rabbit monoclonal, #9234, RRID: AB_2269803, Cell Signaling Technology; 1:1000 for WB), anti-p70 S6K α (S6K)(mouse monoclonal clone H-9 ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... followed by overnight incubation at 4°C with anti-HA antibody (rabbit polyclonal antibody; 11684-T62, Sinobiological) and anti-mouse β-actin antibody (mouse monoclonal antibody; 3700, Cell Signaling) as primary antibodies ...
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bioRxiv - Physiology 2023Quote: ... Membranes were washed in TBST and incubated with HRP - conjugated anti-rabbit IgG at 1:2000 dilution in blocking solution (#7074, Cell Signaling Technology). Bands were revealed with ECL Prime reagent (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... then stained with 1:2000 chicken anti-GFP (Aves #GFP-1020) for visualization of dendrites and 1:3200 rabbit anti-cFos (Cell Signaling 9FG) to validate stimulation ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... membranes were washed in TBST and incubated for 60 minutes with rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Cell Signaling Technology 7074) diluted at 1:2000 in 5% milk-TBST ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was further incubated for 1 h at room temperature with secondary goat anti-rabbit horseradish peroxidase antibody (Cell Signaling Technology) at 1:10,000 in 5% BSA-TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Western blots were performed using rabbit monoclonal antibody against Abcb1 (clone E1Y7S) and rabbit polyclonal antibody against Lrp1 (Cat.-No.: 64099) (both antibodies were bought from Cell Signaling Technology), as well as rabbit polyclonal antibody against claudin-5 (Cat.-No. ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryos were incubated in rabbit anti-phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody (1:200, catalog # 3678, Cell Signaling Technology) in 1% BSA/PBST overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were then washed 3 times with PBST and were incubated with HRP-conjugated anti-rabbit IgG (1:500 dilution, catalog 7074S, Cell Signaling Technology) for 1 hr ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The mouse anti-rabbit (#3678S) and the anti-mouse HRP-conjugated secondary antibodies (#7076S) were from Cell Signaling Technology (Beverly, MA, USA). Enhanced chemiluminescence was used for detection (Amershan Bioscience ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... at a 1:3000 dilution in TBS-T and rabbit polyclonal anti-Phospho-p70 S6K(Thr389) antibodies (#9205, Cell Signaling Technology, USA) at a 1:1000 dilution in TBS-T ...
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bioRxiv - Immunology 2024Quote: ... Horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse secondary antibodies were used to assess protein signal (Thermo Scientific, Cell Signaling Technologies). Chemiluminescence was measured using an Odyssey Fc Imaging System (Licor ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed with TBS-T before staining with secondary anti-rabbit IgG (H+L) DyLight 800 4X PEG Conjugate (Cell Signaling #5151) at 1:20,000 at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated overnight with primary antibody (1:1000 diluted in blocking serum, monoclonal anti-rabbit DCX; Cat#4604S, Cell Signaling Technology) on a shaker (Model# BDRAA115S ...