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Citations for Cell Signaling Technology :
8051 - 8100 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... At least 1 mg of proteins were immunoprecipitated with mouse anti-Ezrin (Novex, 357300) and rabbit anti-EGF receptor (Cell Signaling 4267) in rotation at 4° C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... After incubation with the primary antibodies (mouse anti-firefly luciferase, clone Luci17; ab16466, Abcam; rabbit monoclonal anti-mouse CD8, 989415, Cell Signaling Technology; rabbit anti-NK1.1/CD161 ...
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bioRxiv - Neuroscience 2024Quote: ... after which they were incubated overnight at 4°C with rabbit anti-c-fos (1:500, Cell Signaling Technology, catalog no. 2250S). Sections were then washed with PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were rinsed three times for 10 minutes and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse antibodies (1:2,500, Cell Signaling, #7074, #7076) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... The proteins in the gel were then electrotransferred to polyvinylidene (PVDF) membranes and immunoblotted using an antibody against Psma2 (1:1000 dilution [rabbit]; Cell Signaling #2455) or Rpt6 (1:1000 dilution [mouse] ...
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bioRxiv - Bioengineering 2024Quote: ... Hydrogels were stained with primary antibodies (mouse anti-KRT7, 1:300, NSJ Bioreagents catalog #V2656; rabbit anti-Ki67, 1:400, Cell Signaling catalog #9129 ...
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bioRxiv - Bioengineering 2024Quote: Membranes were subsequently incubated overnight at 4°C with the following primary antibodies: rabbit anti-GAPDH (1:1,000, Cell Signaling Technology #2118) or rabbit anti-HTT ...
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bioRxiv - Bioengineering 2024Quote: ... the membranes were washed three times with 1x TBS-T and incubated with HRP-conjugated goat anti-rabbit antibodies (1:2,500, Cell Signaling Technology #7074P2) in blocking solution for 1 hr at room temperature ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... and incubated with primary antibodies at 1:1000 dilution (overnight at 4°C): rabbit polyclonal anti-phospho (Thr202/Tyr204) Erk1/2 (#9101; Cell Signaling Technology), rabbit polyclonal anti-Erk1/2 (#9102 ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... cells were incubated in the blocking solution with the HRP linked anti-mouse or anti-rabbit secondary antibody (respectively #7076 or #7074; Cell Signaling Technology) at 1:10,000 dilution (1 hr at RT in the dark) ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% milk and incubated with primary antibodies overnight and incubated with anti-rabbit secondary antibody from Cell Signaling Technologies for one hour after which blots were developed using Bio-Rad ChemiDoc™ Imaging Systems ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked with 5% skim milk in PBS containing 0.05% Tween 20 and then incubated with rabbit anti-RhoA (1:1000, Cell Signaling Technology, 67B9), rabbit anti- Pten (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Separate sets of slides are stained for imaging in each fluorescent channel with an anti-PSMA rabbit primary antibody (D4S1F, Cell Signaling Technology) and either an IRDye680LT goat anti-rabbit (LI-COR ...
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bioRxiv - Immunology 2024Quote: ... or phospho ACC S79 (clone D7D11 cat no. 11818) prior to incubation with anti-rabbit Alexa 647 secondary (cat no. 4414; all Cell Signaling technology). Cells were washed and resupended in 0.5% FBS/PBS (v/v ...
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bioRxiv - Developmental Biology 2021Quote: About 3×108 cells stored in BD Phosflow™ Perm Buffer III were washed twice with 1% PBSA buffer (PBS containing 1% BSA) and then incubated with rabbit anti-SOX1 antibody (Cell Signaling Technology, #4194) at 1:150 dilution for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... the myc (2278) and HA epitopes (3724) and HRP-coupled anti-rabbit secondary antibody as well as Normal Donkey Serum from Cell Signaling Technology (CST); the FLAG epitope from CST (2368 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The membranes were blocked for 2 hours at room temperature and then incubated with rabbit monoclonal anti-pERK1/2 antibody (Cell Signaling Technology, USA) and anti-rabbit horseradish peroxidase-conjugated secondary antibody (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2021Quote: ... The following primary and secondary antibodies were used in WB including anti-ICAM1 rabbit antibody (Cell Signaling Technology, Cat. No. 4915S, Danvers, USA), anti-RAB5C rabbit antibody (Thermo ...
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bioRxiv - Molecular Biology 2019Quote: ... Following 3 washes with TBS-T the immunoblot was incubated for 1h with an rabbit IgG HRP-linked antibody (1:1,000; Cell signaling technology, Danvers, MA) in TBS-T ...
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bioRxiv - Neuroscience 2019Quote: ... For surface immunostaining of endogenous HA-Nrxn1α live mouse cortical Nrxn1αHA/HA KI neurons were incubated with rabbit anti-HA (1:100 dilution; Cell Signaling Technology, Cat #3724) diluted in conditioned neuronal culture medium for 20 min at room temperature ...
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bioRxiv - Physiology 2020Quote: ... followed by immersion in 0.3% glycine for 10 min and placed in 0.03% SDS for 10 min then stained with primary IRE1-1α (anti-rabbit, Cell Signaling Tech, 1:100) antibody overnight ...
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bioRxiv - Genomics 2021Quote: ... For profling the SEC and Dotcom components via manual and and AutoCUT&Tag we used rabbit anti-ENL (Cell Signaling Tech Cat# 14893S), rabbit anti-ELL (Cell Signaling Tech Cat# 14468S ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies used were horse anti-mouse HRP or goat anti-rabbit HRP which were both diluted 1:1,000 in block (Cell Signaling Technology, Danvers, MA).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RRID:AB_11179210), and anti-rabbit IgG antibody conjugated to horseradish peroxidase (HRP) (Cat# 7074, RRID:AB_2099233) were acquired from Cell Signaling Technology (Danvers, MA, USA). Anti-GAPDH (Cat# NB600-502 ...
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bioRxiv - Cell Biology 2021Quote: ... Washed membranes were incubated with HRP-conjugated anti-rabbit or anti-mouse secondary antibodies as indicated (1:2000, Cell Signaling Technology, Beverly, MA) in 10 ml of blocking buffer with gentle agitation for 1 h at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with rabbit anti-phosphorylated ERK1/2 antibody (Cell Signaling Technology; 9101S; LOT: 30; 1:1,000) in TBST containing 5% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... and sera from individual animals were tested by ELISA with KLH-conjugated peptide immobilized on plastic surface and anti-mouse or anti-rabbit horse radish peroxidase (HRP)-conjugated detector Ab (Cell Signaling Technology, USA). The animals with the highest Ab titer were selected for booster immunization ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were probed overnight at 4°C using primary antibodies against p-eIF2α S51 (rabbit, Cell Signaling cat no: 9721, 1:500), anti-t-eIF2α (rabbit ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated for one hour with horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG (Cell Signaling Technology; catalog #’s: 7076 and 7074). Membranes were then washed three times in TBST only (five minutes per wash) ...
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bioRxiv - Immunology 2021Quote: ... Fixed cells were stained with rabbit anti-mouse COX-2-Alexa488 at 1:100 dilution in perm wash buffer (Cell signaling, cat #13596S) for 1 hr at RT ...
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bioRxiv - Cell Biology 2021Quote: ... Sonicated chromatin was diluted and added to 0.2 ml PCR tube containing primary antibodies (3ug of rabbit anti-NKX6.1 cat. 54551 (Cell Signaling Technology, Danvers, MA, USA); 3 μg of rabbit-anti-acetyl-histone H3-cat ...
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bioRxiv - Genomics 2022Quote: Antibodies used for CUT&Tag or MulTI-Tag in this study were as follows: Rabbit Anti-H3K27me3 (Cell Signaling Technologies CST9733S, Lot 16), Mouse anti-RNA PolIIS5P (Abcam ab5408 ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Immunoblotting analysis was performed with the following monoclonal primary antibodies: rabbit anti p-mTOR (1:1000; #5536, Cell Signaling Technology, Danvers, MA, USA) and rabbit anti mTOR (1:1000 ...
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bioRxiv - Microbiology 2022Quote: Rabbit anti-α-tubulin was purchased from Sigma (cat# SAB3501072) and goat anti-rabbit IgG HRP-conjugated and goat anti-mouse IgG HRP-conjugated were purchased from Cell Signaling (cat# 7076S).
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bioRxiv - Microbiology 2022Quote: ... The fluorescent-labelled Alexa Fluor 647 (AF647) goat anti-Rabbit IgG monoclonal antibody was from Cell Signaling Technologies (MA, USA; Cat. n° 4414).
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bioRxiv - Cancer Biology 2022Quote: ... and horseradish peroxidase (HRP)-conjugated rabbit and mouse secondary antibodies for immunoblot and MitoTracker Red CMXRos for immunofluorescence purchased from Cell Signaling Technologies (USA). Mouse monoclonal primary antibodies against p53 (DO-1) ...
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bioRxiv - Immunology 2022Quote: ... the membrane was incubated for one hour at RT with peroxidase-conjugated goat anti-rabbit secondary antibody (Cell Signaling Technology, #7074, 1:1000) and washed again ...
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bioRxiv - Cell Biology 2022Quote: ... 50% of ‘input’ and ‘immunoprecipitated’ fractions were resolved by SDS-PAGE and immunoblotted with rabbit anti-myc (1:3000, 71D10, Cell Signaling Technology, 2278S) and mouse anti-GFP (1:3000 ...
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bioRxiv - Neuroscience 2021Quote: ... staining was carried out using rabbit polyclonal antibody phospho-PAK1 Thr432/ Pak2 Thr402 directed against a synthetic phosphopeptide (Cell Signaling Technology# 2601, RRID:AB_330220). This antibody recognizes phospho-PAK1,2 to a greater extent than PAK3 ...
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bioRxiv - Neuroscience 2020Quote: ... transferred onto nitrocellulose membranes and detected by immunoblot using the following primary antibodies: mGlu5 (rabbit clone D6E7B from Cell Signaling, dilution 1:1000), actin (mouse clone from DSHB ...
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bioRxiv - Neuroscience 2020Quote: ... then blocked with 3 % BSA in PBS for 1 h at room temperature and incubated with primary antibodies (mouse anti-GFAP, Sigma, 1:500; mouse anti-NeuN, Millipore, 1:500; rabbit anti-cleaved caspase-3, Cell Signaling, 1:500) overnight in 3 % BSA in PBS at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated for 48h at 4°C with the following primary antibodies: rabbit anti-phospho-S6 (1:1000, Cell Signaling, Cat# 5364), mouse anti-NeuN (1:400 ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies against phospho-STAT1 and STAT1 and horseradish peroxidase-conjugated goat anti-rabbit antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA).
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bioRxiv - Neuroscience 2021Quote: Primary antibodies for western blots were diluted as follows: rabbit anti-Phospho-AKT Ser473 (D9E) (Cell Signaling Technology, USA; RRID: AB_2797780; 1:2000), mouse anti-Pan-AKT (40D4 ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A was washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling Technology, Cat# 12040) or 5ug Normal Rabbit IgG Polyclonal Antibody (Merck ...
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bioRxiv - Cancer Biology 2020Quote: ... 50 μL of empty Dynabeads® Protein A were washed by Citrate-Phosphate Buffer (pH 5.0) and then incubated with 15ul Rabbit Monoclonal anti-UPF1 antibody (Cell Signaling TECHNOLOGY, Cat# 12040) or 5ug Rabbit polyclonal anti-phospho-Upf1 (Ser1127 ...
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bioRxiv - Neuroscience 2021Quote: ... the retinal explants were immuno-stained against microtubule with beta-3 tubulin (TUBB3) antibody (1:250, from rabbit, Cell Signaling Technology, 5568S, USA), washed in 0.5% Triton in PBS for 5 times ...
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bioRxiv - Cancer Biology 2020Quote: ... for 2 hours and blotted at 4°C overnight with primary antibodies as follows: rabbit anti-phospho-GSK-3β (Ser9) (5B3) (1/500 dilution; Cell Signaling; cat #9323), rabbit anti-GSK-3β (1/1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... the goat anti-mouse (polyclonal 7076) and goat anti-rabbit (polyclonal 7074) peroxidase-conjugated secondary antibodies were from Cell Signaling (Beverly, MA. USA). DMEM complete medium ...