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Citations for Cell Signaling Technology :
8101 - 8150 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cells were blocked for 1h with 5% BSA in PBS and then stained with rabbit anti-pPLCγ1 antibody (Cell Signaling, #2821, 1/100) overnight at 4C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed with incubate for 1 hour at room temperature in 1:500 goat anti-rabbit Alexa Fluor 555 (Cell Signaling Technology, 44135), 1:400 Alexa Fluor 488 Phalloidin (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed on 6% acrylamide SDS-PAGE gels followed by immunoblotting with rabbit anti-myc (1:3000, 71D10, Cell Signaling Technology, 2278S). In vitro dephosphorylation experiments were performed in three biological replicates.
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bioRxiv - Developmental Biology 2022Quote: ... MilliporeSigma, Cat# V8012), Rabbit anti-HA antibody (1:1000, MilliporeSigma, Cat# H6908) and Mouse anti-Myc antibody (1:1000, Cell Signaling, Cat# 2276S) as primary antibody ...
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bioRxiv - Immunology 2019Quote: ... Permeabilized cells were washed three times with D-PBS/5% FBS and processed for intracellular staining for p(Y694)-STAT-5 (D47E 7 XP® rabbit, Cell Signaling Technology) in D-PBS/5% FBS for 1 h at room temperature ...
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bioRxiv - Genetics 2019Quote: ... and the primary antibodies were recognized by Alexa Fluor® 594 Donkey anti-rabbit IgG (H+L) (ANT030, Antgene) or Alexa Fluor® 555 Anti-mouse IgG (H+L) (4409S, Cell Signaling Technology) and shaken at room temperature for 1.5 hrs in the dark ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were incubated overnight with one of the following rabbit primary antibodies (all from Cell Signaling Technology and all cross-react with rat): anti-Pan-AKT (1:1000 ...
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bioRxiv - Systems Biology 2021Quote: ... We incubated the cell lysates with Phospho-YAP (Ser397) rabbit antibody and performed a sandwich-ELISA assay by using PathScan Phospho-YAP (Ser397) Sandwich ELISA Kit (Cell Signaling Technology, #57046). We then used a Synergy HTX Multi-Mode Reader (BioTek ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against pSTAT3 and pAKT were detected by using a conformation-specific HRP-conjugated anti-rabbit IgG secondary antibody (1:4,000; Cell Signaling Technologies; RRID: AB_10892860). Antigen-antibody complexes were visualized by using enhanced chemiluminescence substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% skimmed milk and incubated with primary antibodies (rabbit anti-PPAR-γ antibody, Cell Signaling Technology, 1:1000) and HRP-conjugated goat anti-rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... the samples were separated by SDS–polyacrylamide gel electrophoresis (PAGE) and analyzed by Western blotting for Actin using anti– pan-Actin polyclonal rabbit Ab (1:2000; Cell Signaling Technology, 4968).
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bioRxiv - Neuroscience 2019Quote: DREADDs animal brain sections were incubated overnight at room temperature in rabbit anti-HA (for detection of HA-Tag; 1:500; Cell Signaling Technology, MA) and mouse anti–dopamine beta hydroxylase (DBH ...
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bioRxiv - Cell Biology 2020Quote: ... of embedded organoids or whole-mounted organoids were incubated overnight at 4°C with rabbit monoclonal anti-p63a antibody (D2K8X, Cell Signaling Technology, MA), polyclonal guinea pig anti-cytokeratin 8/18 (03-GP11 ...
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bioRxiv - Neuroscience 2019Quote: ... the membranes were washed and incubated with either secondary goat anti-rabbit IgG or goat anti-mouse IgG at 1:5,000 dilution (Cell Signaling Technology, Danvers, MA) at room temperature for 90 min ...
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bioRxiv - Genetics 2021Quote: ... Membranes were rinsed with TBST for 30 min before incubation with horseradish peroxidase-conjugated anti-rabbit secondary antibody (Cell Signaling; 7074S, 1:5000) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were blocked at room temperature with 1% BSA for 1 h and incubated overnight at 4 °C with primary antibodies: rabbit Collagen I antibody (Cell Signaling Technology, 84336), mouse E-cadherin antibody (Abcam ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was washed 1× TBST three times for 10 min each followed by incubation with anti-rabbit IgG horseradish-peroxidase secondary antibody conjugate (1:3000 dilution; Cell Signaling Technology, 7074S) in 1× TBS for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein bound beads were boiled with 1X SDS loading buffer and resolved in 10% SDS-PAGE and analyzed by with 1:1000 dilution of rabbit anti-PAX9 polyclonal antibody (Cell Signaling Technology, USA) or 1:1000 dilution of mouse anti-FLAGM2 monoclonal antibody (Sigma-Aldrich ...
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bioRxiv - Physiology 2021Quote: ... 0.4% Triton and 1% BSA in KPBS for 20 minutes and then incubated in rabbit-α-pSTAT3 (1:500; 9145 from Cell Signaling Technology) or mouse-α-Oxytocin (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were probed overnight at 4°C in Odyssey Blocking Buffer with antibodies recognizing PS6 (Ser240/244; rabbit monoclonal; 1:1000; Cell Signaling, Danvers, MA), total S6 ribosomal protein (mouse monoclonal ...
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bioRxiv - Microbiology 2021Quote: ... Rabbit monoclonal anti-phospho-TANK Binding Kinase 1 (TBK1) (#5483) and anti-TBK1 (#3013) were purchased from Cell Signaling Technology (Beverly, MA, USA). Monoclonal antibody against NDV nucleoprotein (NP ...
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bioRxiv - Molecular Biology 2021Quote: ... 9ug/ml) obtained from LS Bio (Seattle,WA) followed by horseradish peroxidase linked anti-rabbit secondary antibody (Cell Signaling Technology Inc., Beverly, MA). Slides were developed with DAB substrate ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit monoclonal anti-SorLA antibody specific to the N-terminus of human SorLA protein (1:1,000; D8D4G #79322; Cell Signaling, Danvers, MA, USA) for the detection of SorLA759 proteins ...
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bioRxiv - Microbiology 2021Quote: ... was used for western blotting at a dilution of 1:1,000 and rabbit polyclonal antiserum reactive against protein kinase A (PKA) substrates (Cell Signaling Technology, Danvers, MA) was used for western blotting at a dilution of 1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Blocked membranes were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rabbit anti-Ezh2 (Cell Signaling Technologies; 1:1000), rabbit anti-H3K27me3 (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... The slides were incubated overnight with the following primary antibodies at the indicated dilutions: rabbit monoclonal anti-HA 1:1000 (Cell Signaling Technology 3724), rabbit polyclonal anti-PTEN 1:400 (Thermo Fisher 51-2400) ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1% Tween-20 in 1x TBS) and then treated with primary antibodies (YAP/TAZ, anti-rabbit, Cell Signaling, 8418 and beta-Actin, anti-mouse, Cell Signaling, 3700) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... for 2h then incubated with primary antibodies (1:5000) overnight: anti-HA antibody (rabbit polyclonal antibody; Sinobiological #11684-T62) and anti-β-actin antibody (mouse monoclonal antibody; Cell Signaling #3700). The membrane was washed with TBS – Tween 20 0.1% ...
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bioRxiv - Physiology 2022Quote: ... Cat # G9545, 37 kDa) or ACTB Rabbit polyclonal antibody (1:10,000 dilution, Cat #4970, 37 kDa) was purchased from Cell signaling (Danvers, MA, USA). Proteins were visualized using infrared secondary antibodies (1:10,000 ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 4x with PBST for 5 minutes each and incubated for 1 hour in the dark at room temperature with anti-rabbit IgG HRP-linked secondary antibody (Cell Signaling, Danvers, MA) diluted 2000-fold in 5% milk ...
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bioRxiv - Microbiology 2022Quote: ... and then incubated at room temperature with HRP-conjugated secondary antibodies ((goat anti-rabbit HRP conjugated, Cell Signaling, Cat #: 7074, 1:5,000 dilution) (goat anti-mouse HRP conjugated ...
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bioRxiv - Neuroscience 2022Quote: ... To detect changes in IL-6 signal transduction we used primary monoclonal rabbit anti-Stat3α (D1A5) from Cell Signaling Technologies (Cat no. 8768S) and used the same secondary anti-rabbit antibodies described above.
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bioRxiv - Neuroscience 2022Quote: ... and then immunoprecipitated for 3 hours at 4°C by agarose beads preincubated with rabbit anti-HTT D7F7 antibody (Cell Signaling, Cat#5656). To remove the non-specific binding ...
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bioRxiv - Neuroscience 2022Quote: ... blots were incubated for two hours in 5% milk in 1X TBST for blocking followed by an overnight incubation with rabbit anti-c-Fos antibody (1:1000, Cat. No. 2250, Cell Signaling Technology, USA) at 4°C ...
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bioRxiv - Physiology 2022Quote: ... for 1 hour at RT and then incubated O/N with primary antibody for c-Fos (Rabbit anti-cFos, Cell Signaling, Cat# 2250) in blocking solution at RT ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-otoferlin (Developmental Studies Hybridoma Bank, HCS-1) diluted at 1:100 and rabbit anti-cleaved caspase-3 (Cell Signaling Technology, 9661) diluted at 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... and 2 h later they were labeled with mouse anti-Iba1 (1:500; Fujifilm Wako Chemicals, VA, USA) or rabbit anti-p75NTR (1:750; Cell signaling, MA, USA) overnight ...
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bioRxiv - Neuroscience 2024Quote: ... The incubation was performed using the free-floating method at 4°C (48 h) with rabbit anti-p75NTR(1:500; Cell signaling, MA, USA) with 0.6% Triton X-100 in PBS with 5% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated with anti-human N-Myc rabbit monoclonal antibody (#51705, Cell Signaling Technology, Inc., MA, USA at a dilution of 1:300) and S100 beta (EP32 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by incubation for 1 hour at room temperature in the dark in with secondary antibodies (Anti-rabbit: IgG DyLight 800, Anti-mouse: IgG DyLight 680 (Cell Signaling Technologies, USA)) ...
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bioRxiv - Genetics 2024Quote: ... Horseradish peroxidase(HRP)–conjugated streptavidin (streptavidin-HRP; Cat# 3999S) and HRP-conjugated rabbit monoclonal antibody against β-actin (Cat# 5125S) were obtained from Cell Signaling Technology. HRP-conjugated mouse monoclonal antibody against FLAG was obtained from GenScript (Cat# A01428).
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bioRxiv - Biochemistry 2023Quote: ... were used at a concentration of 1:1000 and visualized using HRP-conjugated secondary antibodies (anti-rabbit [Cell Signaling, 7074S] or anti-mouse [Cell Signaling, 7076S]) at a concentration of 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were detected using goat anti-mouse or anti-rabbit IgG (H+L) Alexa fluor 488 or 647 (Cell signaling Technologies/ Bioconcept) secondary antibodies diluted 1:500 in 1% BSA 0.1% Triton-X 100 in PBS for 3 hours in the dark at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... β-actin [8H10D10] primary antibodies and anti-rabbit-HRP or anti-mouse-HRP antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). IRF3 [A19717] were purchased from ABclonal (Woburn ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed by 1× TBST 3 times and incubated with a secondary anti-rabbit-HRP or anti-mouse antibody-HRP (Cell Signaling, Danvers, MA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... To confirm the specificity of staining cells were incubated with isotype mouse (7076P2) or rabbit (7074P2) IgG antibodies (Cell Signaling, Danvers, MA, USA) (Fig ...
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bioRxiv - Immunology 2023Quote: ... Membranes were incubated for 1 h at room temperature with appropriate secondary antibodies: goat anti-rabbit (1:2000, Cell Signaling Technology, Cat. 7074), goat anti-rat (1:10000 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with anti-rabbit IgG (H + L) F (ab′)2 fragment (Alexa Fluor® 488 conjugate) (4412s, 1:200, Cell Signaling Technology) for 3 h at 25°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Primary staining was performed on separate blots with the following antibody dilutions in 5% BSA/TBST: rabbit anti-EZH2 1:1000 (Cell Signaling Technology, 5246S), rabbit anti-CBX2 1:500 (Bethyl Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... Blots were washed in TBS containing 0.1 % Tween20 and labeled with horseradish peroxidase (HRP)-conjugated secondary anti-mouse or anti-rabbit antibody (Cell Signaling Technology, Beverly, MA). Proteins were enhanced by chemiluminescence (Amersham ECL plus Western Blotting detection system ...