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Citations for Cell Signaling Technology :
7951 - 8000 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Blots were then incubated in HRP-linked secondary antibody against mouse or rabbit IgG (1:3000, Cell Signaling, Cat# 7074S and 7076S) in 4% BSA for 1 hr at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Depletion of ATGL16L1 and LC3B was verified on ALI 7 days by immunoblot analysis using antibodies to rabbit anti-ATG16L1 (1:1000 vol;vol ATG16L1 1:1000 vol;vol (Cell Signaling D6D5) or rabbit anti-LC3B (1:500 vol;vol Sigma-Aldrich L7543) ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 µg/mL of aprotinin and leupeptin] and incubated (16 hrs., 4°C) with either 3 µg non-immune control rabbit IgG (Cell Signaling #272) or with 3 µg of ERRα antibody (Cell Signaling #13826 ...
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bioRxiv - Neuroscience 2022Quote: ... then overnight in antibody solution containing 2% normal donkey serum and 0.3% Triton X-100 in PBS with primary rabbit anti-activated caspase 3 antibody (Cell Signaling, Cat# 9661). Sections were washed five times for five minutes in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed and processed for immunofluorescence as described above with a rabbit ɑ-FLAG antibody (1:1000, Cell Signaling Technologies, D6W5B) to visualize BicD2-KIF fusions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the primary antibody solution contained a 1/1000 dilution of a monoclonal rabbit anti-HA antibody (C29F4, Cell Signaling Technology, Danvers, USA) in Tris-buffered saline (TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The immunoblots were then incubated with secondary antibody for 1 h at room temperature (1:1000 dilution, anti-rabbit Cat. #7074, lot 28, or anti-mouse HRP-conjugate Cat. #7076S, lot 32, Cell Signaling Technology). Specific signal was detected using SuperSignal West Pico Chemiluminescent Substrate (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were washed with Tween-TBS and incubated in secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody #7074 or Anti-mouse IgG, HRP-linked Antibody #7076; both Cell Signaling technology) for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Poly-ADP-ribose polymerase (PARP) antibody and goat anti-rabbit IgG secondary antibody conjugated to horseradish peroxidase (HRP) were acquired from Cell Signaling Technology-Ozyme (Saint-Quentin-en-Yvelines ...
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bioRxiv - Biochemistry 2022Quote: ... Unroofed RBCs were blocked for 30 mins at room temperature in blocking buffer and then incubated with 1:200 rabbit anti-HA primary antibody (3724; Cell Signaling Technology) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and permeabilized with 0.3% Triton X-100 in PBS (PBST) prior to blocking and staining with rabbit anti-acetylated alpha tubulin antibody (Cell Signaling #5335). Confocal 3D images of whole islets were used for cilia frequency and length quantitation ...
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bioRxiv - Immunology 2022Quote: ... primary antibody pairs against NFκB total (rabbit-anti-NFκB p65 total, Cell Signaling Technology #8242; mouse-anti-NFκB p65 total, Cell Signaling Technology #6956); NFκB phospho (rabbit-anti-NFκB p65 total ...
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bioRxiv - Immunology 2022Quote: ... The following horseradish peroxidase (HRP) conjugated secondary antibodies were used for this study: mouse anti-rabbit IgG (L27A9) monoclonal antibody (Cell Signaling #5127), goat anti-rabbit IgG polyclonal antibody (Cell Signaling #7074S) ...
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bioRxiv - Microbiology 2022Quote: ... The primary antibody was diluted to 0.1 mg/mL and anti-rabbit IgG HRP linked secondary antibody diluted 1:1000 (Cell Signaling Technology; 7074S).
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bioRxiv - Immunology 2022Quote: ... and a secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (1:1000 in blocking buffer for 1 hour, Cell Signaling Technology 7074S) with the peroxidase substrate KPL TrueBlue (30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated for 8 min at 37 °C with primary antibodies against HA (rabbit, 1:250, Cell signaling Technology, cat #C29F4). After washes ...
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bioRxiv - Physiology 2023Quote: ... Bound primary antibodies were detected using horseradish peroxidase-conjugated goat anti-mouse or -rabbit secondary antibodies (Cell Signaling Technology, Inc., Danvers, MA), and protein signals were visualized using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Our experimental sample used TgBDP13xHA parasites and 1ul of a 1:50 dilution of the rabbit anti-HA primary antibody (Cell Signaling 3724T). The negative control sample used ΔKu80 parasites (the parental line ...
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bioRxiv - Physiology 2022Quote: ... The membranes were subsequently washed 4 times in TBS-T for 10 min before incubating in the appropriate secondary antibody anti-rabbit HRP (1:5000; 1677074P2, Cell Signaling Technology) or anti-mouse HRP (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... The following day sections were washed in wash buffer 3 times for 5 min and co-stained for anti-Rabbit IgG Fab2 Alexa Fluor 488 (Cell Signaling, 4412S) at 1:1000 dilution at room temperature for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins captured on the magnetic beads were eluted with 2X SDS loading dye for 5 min and analyzed by Western Blotting using rabbit anti-FLAG (14793S; Cell Signaling Technology) and anti-GFP (A-6455 ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-phospho-p70 S6 kinase (Thr389) (clone 108D2, #9234) and anti-LC3A/B (clone D3U4C, #12741) were rabbit monoclonal antibodies from Cell Signaling Technology. Anti-Akt (#9272 ...
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bioRxiv - Neuroscience 2022Quote: ... One set of tissue sections from each rat (with each set containing a complete rostrocaudal series of sections spaced by 210 μm) was incubated in a rabbit polyclonal antiserum against cFos (1:10,000; Cell Signaling, 2250; AB_2247211), followed by biotinylated donkey anti-rabbit IgG (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-Rabbit Secondary Antibody in 1:30000 dilution and Anti-Mouse Secondary Antibody (Cell Signaling Technology, 5151; Li-Cor, 926-68020, respectively) were used in 1:15000 dilution during Western Blotting experiments.
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bioRxiv - Biophysics 2022Quote: SnapTag and HaloTag Janelia Fluor dyes were obtained from the Janelia Research Campus.54 Rabbit monoclonal antibodies against EEA1 and against LAMP1 were from Cell Signaling Technologies (Catalog # ...
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bioRxiv - Developmental Biology 2022Quote: ... Blots were washed and incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody generated in Rabbit (1:2000; Cell Signaling Technology #7074) for 1.5 hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... membranes were washed in TBST and incubated for 60 minutes with rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Cell Signaling Technology 7074) diluted at 1:2000 in 5% milk-TBST ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were diluted in PBS-T containing 5% BSA and used 1:1000 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058), 1:1000 for anti-phospho-Akt(Thr308 ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibody solution contained goat anti-mouse antibodies FITCI at 1:1000 dilution and goat anti-rabbit dilution TRITCI at 1:1000 dilution (from Cell Signaling Technologies) and incubated in humidity chambers at 37°C for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... and 5% non-fat milk for 60 minutes at room temperature and incubated with primary monoclonal antibody (1:1000 rabbit anti-FLAG, anti-HA, Cell Signaling Technologies) overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... The membrane was blocked with 5% skim milk and probed with primary antibodies overnight at 4°C: either rabbit monoclonal anti-TET2 (Cell Signaling Technology) or monoclonal mouse anti-GAPDH (Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... followed by overnight incubation at 4°C with anti-HA antibody (rabbit polyclonal antibody; 11684-T62, Sinobiological) and anti-mouse β-actin antibody (mouse monoclonal antibody; 3700, Cell Signaling) as primary antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in primary antibodies (1:500 HCS-1 mouse monoclonal, DSHB, University of Iowa; 1:400 anti-cleaved Caspase-3 rabbit polyclonal, Cell Signaling Technology). After overnight incubation in primary ...
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bioRxiv - Immunology 2024Quote: ... then stained at 4°C overnight with various combinations of the following antibodies: rabbit anti-NeuN (1:1000, Cell Signaling Technologies, 12943S), rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... cFos protein was labelled in the same manner using rabbit polyclonal antibody c-Fos (9F6) (1: 1000, Cell Signaling Technology Inc, 2250) and Alexa Fluor 488 AffiniPure F(ab’)□ Fragment Donkey Anti-Rabbit (1:200 ...
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bioRxiv - Cell Biology 2024Quote: ... Crude cell extract was lysed with an insulin syringe and pre-cleared by incubation with 3 μg Normal Rabbit IgG (Cell Signaling Technology) and 10uL Pierce™ Protein A/G Magnetic Beads (Thermo Scientific 88803 ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were blocked in 3% BSA in PBS plus 0.4% Triton X-100 for 30 m at RT and incubated in primary antibody rabbit anti–Calreticulin at 1:100 (Cell Signaling Technology) overnight at 4°C ...
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bioRxiv - Pathology 2023Quote: ... formalin fixed paraffin embedded tissue sections were stained with rabbit-anti claudin-2 (1:100, Cell Signaling Technology Cat# 28530, RRID: AB_2798961), and rat-anti CD45 (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor 488-conjugated rabbit monoclonal anti-phospho-histone H3 (pH3) (1:200 for IF and 1:50 for flow cytometry; D2C8; Cell Signaling Technology); and fluorescence (Alexa Fluor 488 or Alexa Fluor 594 ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were detected secondaries antibodies diluted in PBS with 1% goat serum: polyclonal Alexafluor 594-conjugated anti-rabbit IgG (1:1000, Cell Signaling, 8889), and polyclonal Alexafluor 488-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution using the following concentrations: 1:1000 anti β-actin (13E5) rabbit primary antibody (#4970 from Cell Signaling), 1:1000 anti-tdtomato rabbit primary antibody (#632496 from Takara) ...
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bioRxiv - Cell Biology 2024Quote: The membrane was then incubated overnight at 4 °C with primary antibody solution (1:1000 anti β-actin (13E5) rabbit primary antibody #4970 from Cell Signaling) (1:1000 anti GAPDH ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed 3X with TBST for a total of 15 minutes and incubated with HRP conjugated secondary anti-mouse (7076S) or anti-rabbit (7074S) antibodies at a 1:5000 ratio (Cell Signaling Technology) for 1 hour at room temperature with gentle rocking ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary and secondary antibodies were diluted in FACS buffer and used 1:100 for anti-phospho-Akt(Ser473) (rabbit; Cell Signaling, 4058) and 1:500 for anti-rabbit Alexa-647 (goat ...
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bioRxiv - Cell Biology 2024Quote: ... Specimens were incubated overnight at 4 °C with rabbit anti-SARS-CoV-2 nucleocapsid protein (Cell Signaling Technology, USA, #26369, 1:200), mouse anti-ACE2 (Cell Signaling Technology ...
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were washed three times for five min each with TBST and then incubated with secondary antibodies (goat anti-rabbit IgG HRP, A0545, Sigma Aldrich; goat anti-mouse IgG HRP, 7076; Cell Signaling) for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The membranes were probed overnight at 4°C with rabbit monoclonal antibodies against inducible nitric oxide synthase (iNOS; Cell Signaling Technology, 2982) or arginase-1 (Arg-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... 4×106 cells were fixed in methanol and incubated with anti-pS6 (Ser235/236) rabbit antibody (1:100, Cell Signaling Technology #2211), followed by incubation with cell surface markers and secondary Alexa Fluor 647 goat anti-rabbit (1:200 ...
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bioRxiv - Biophysics 2023Quote: ... the blots were washed 3 times with TBST buffer and shaken gently with 5 mL primary antibody (rabbit anti-MET, #4560, Cell Signaling Technology, 1:1000 dilution ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were then washed 3 times with PBST and were incubated with HRP-conjugated anti-rabbit IgG (1:500 dilution, catalog 7074S, Cell Signaling Technology) for 1 hr ...