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Citations for Cell Signaling Technology :
8151 - 8200 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... the following primary antibodies were used: rabbit polyclonal anti-phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) at 1:250 (Cell Signaling Technology, #3141), rabbit polyclonal anti-phospho-Myosin Light Chain 2 (Ser19 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... anti-Rab11 (1:50; CST, C45B10, #3288) or anti-LAMP1 (1:100; CST, D2D11 XP, #9091) rabbit monoclonal antibodies were purchased from Cell Signaling Technology (CST). Alexa 488 goat anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... and processed for indirect immunofluorescence analysis according to standard protocols using phospho-Histone H2A.X (S139) (20E3) Rabbit antibody (Cell Signaling Technology, 1:1000) and Alexa Fluor 647 goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... one slide was incubated only with Alexa Fluor® 488-conjugated Myc-Tag (71D10) rabbit monoclonal antibody purchased from CST (Cell Signaling Technology). For the measurements ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse-HRP, Cell Signaling #7076; anti-rabbit-HRP, Cell Signaling #7074) in blocking solution (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... The plates were washed three times with PBST and incubated with either 1 μg/ml of rabbit monoclonal anti-mouse TREM2 (Cell Signaling Technology, 76765) for full length Trem2 ELISA (ELISA 1) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then blocked with immunofluorescence (IF) block buffer (3% BSA in PBS) for 1 hour and incubated with primary antibodies: rabbit anti-γH2A.x (Cell Signaling 9718, 1:2000), rabbit anti-RPA32 (GeneTex GTX113004 1:2000) ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were blocked in 2% milk-phosphate-buffered saline (PBS) and incubated with the following antihuman antibodies: rabbit β3-integrin (1:1000; 13166, Cell Signaling Technology [CST]), rabbit p-FAK (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... membranes were treated with an HRP-linked secondary antibody (Anti-rabbit IgG cat. no. 7074S or Anti-mouse IgG cat. no. 7076S; Cell Signaling Technology, Inc) for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS−/−] at room temperature for 1.5 h and subsequently incubated with primary rabbit anti-MPO antibody (Cell Signaling, clone: E1E7I, 1:500) and primary mouse anti-HLA-G antibody (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit anti-SAPK/JNK monoclonal antibody (Cat# 9258, RRID: AB_2141027), and rabbit anti-p-JNK monoclonal antibody (Cat# 4668, RRID: AB_823588) were purchased from Cell Signaling Technology (Danvers, MA, USA). Rabbit anti-ATF2 monoclonal antibodies (Cat# BS1022 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were permeabilized with 0.5% Triton-X in PBS before probing with anti-phospho-histone H2AX (Ser 139) rabbit monoclonal antibody (Cell Signaling Technology cat# 9718) and anti-cyclin A2 (EPR17351 ...
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bioRxiv - Bioengineering 2023Quote: ... Paraffin sections were used for H&E staining as well as sequential double-immuno-staining with rabbit anti-human Ki67 (Cell Signaling Technologies, US) and rabbit anti-human vimentin (Thermo Fisher Scientific ...
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Specific patterns of neural activity in the hippocampus after massed or distributed spatial trainingbioRxiv - Neuroscience 2023Quote: ... After 1 h of incubation in PBST containing 1% BSA and 1% NGS sections were incubated overnight in anti-phospho-c-Fos rabbit monoclonal antibody (5348S; Cell signaling Technology, USA) diluted 1:8000 in PBST– BSA–NGS ...
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bioRxiv - Plant Biology 2023Quote: ... The membrane was blocked for 1 h at room temperature with 5 % BSA dissolved in TBS-Tween (0.1 %) and incubated with the primary antibody rabbit anti-phospho-threonine [(Cell Signaling Technology®, #9381) 1:1000] ...
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bioRxiv - Immunology 2023Quote: ... blots were also probed with an HRP-labelled rabbit anti-ß-actin monoclonal antibody at a 1:1000 dilution (#5152; Cell Signaling Technology). Western blots were visualized with SuperSignal West Pico Chemiluminescent reagents (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Cells were proceeded for IF as described above using anti-CD56 mouse antibodies (1:10, Coulter #PN4235479-G) and anti-TCF7L2 rabbit antibodies (1:100, Cell Signaling Technology #C48H11), revealed by Cy3-coupled anti-mouse IgGs and Cy5-coupled anti-rabbit IgGs.
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used at the indicated dilutions: rabbit polyclonal anti-phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) at 1:50 (Cell Signaling Technology, #3141), rabbit anti-Msn M1 at 1:750 (gift from Thomas Moss ...
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bioRxiv - Cancer Biology 2023Quote: ... These membranes were subsequently probed with the indicated primary antibodies and incubated with appropriate goat anti-rabbit IgG or goat anti-mouse IgG (Cell Signaling Technology, USA) secondary antibodies conjugated with horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2023Quote: ... blots were washed in 1x TBST and incubated with appropriate HRP-conjugated secondary (Goat anti-rat 7077, Cell Signaling, Goat anti-mouse 7076, Cell Signaling, Goat anti-rabbit 7074, Cell Signaling) for two hours with 2% milk powder in TBST ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were rat anti-ATF4 (1:200, (Hunt et al., 2019)) and rabbit anti-P-eIF2α (1:500, anti-Phospho-eIF2α [Ser51], Cell Signaling Technology 9721). Secondary antibodies were goat anti-rabbit Alexa Fluor 546 (Invitrogen A11035 ...
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bioRxiv - Immunology 2023Quote: ... The membranes were then washed three times with TBST and incubated with HRP-linked anti-rabbit IgG antibody (Cell Signaling Technology, catalog # 7074) in TBST containing 5% BSA for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... the membranes were incubated with peroxidase-conjugated secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody, Cell Signaling #7074; Anti-mouse IgG, HRP-linked Antibody, Cell Signaling #7076) and developed with a luminol-based enhanced chemiluminescence (ECL ...
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bioRxiv - Bioengineering 2023Quote: ... secondary antibodies were incubated for 1 hour at room temperature (Secondary antibodies: Anti-Rabbit HRP-conjugated Cell Signaling Technology (CST-#7074S 1:5000); Anti-Mouse HRP-conjugated (R&D ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates and corresponding supernatants were analyzed on the same reducing SDS polyacrylamide gel and detected by Western blot analysis by using rabbit-α-Shh antibodies (Cell signaling C9C5), rabbit-α-GAPDH antibodies (Cell Signaling ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated overnight at 4 °C with rabbit anti-c-fos primary antibody (1:500; Cell Signaling Technology, catalog no. 2250, USA) in a permeable buffer containing 2% donkey serum ...
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bioRxiv - Cell Biology 2024Quote: ... The membranes were incubated with the appropriate primary antibody overnight followed by horseradish peroxidase-conjugated anti-rabbit (#7074, Cell Signaling Technology, Danvers, MA) secondary antibody at a dilution of 1:2500 for an hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... The sections were washed twice with PBS and incubated in 2% BSA plus TBS with primary antibody [1:80 dilution rabbit anti-5-mC antibody (Cell Signaling Technology, #D3S2Z) at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... and the mouse anti-rabbit (#3678S) and the anti-mouse HRP-conjugated secondary antibodies (#7076S) were from Cell Signaling Technology (Beverly, MA, USA).
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bioRxiv - Molecular Biology 2024Quote: ... and immunoblotting experiments were carried out on 10 μL of resultant IP preps whereby polyubiquitinated MyHC fragments were probed using a polyclonal rabbit IgG antibody (1:1000; Cell Signaling; cat#: 3933). In addition to these IP experiments ...
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bioRxiv - Genomics 2024Quote: ... with primary antibody (0.25 µL polyclonal rabbit αHP1 [11]; 0.5 µg αH3K9me3, Abcam 8988; 0.5 µL αBiotin (Cell Signaling Technology # D5A7; for DiBioCUT&Tag) or 0.5 µg normal rabbit IgG (MERCK ...
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bioRxiv - Immunology 2024Quote: ... Vero cells were also infected with RSV-mkate2 at an MOI of 0.1 for 48h in the presence of either rabbit IgG (Cell signaling, catalog no. 2729) or IgG antibodies against ISG15 ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid-bound protein was run on 4-20% SDS-PAGE gel and immunoblotted with His-tag antibody (rabbit monoclonal, Cell Signaling Technology, 2365). Blots were visualized on a Li-Cor Odyssey imaging system ...
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bioRxiv - Neuroscience 2024Quote: ... All sections were incubated overnight at 4⁰ C with rabbit anti-c-Fos primary antibody (5348, 1:2000; Cell Signaling Technology, RRID: AB_10557109) diluted in 2% NHS in PBS-Tx.
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bioRxiv - Physiology 2021Quote: ... which was blocked with 5% BSA in PBS containing 0.1% Tween-20 (0.1% PBST) and incubated with rabbit anti-pAkt antibody (Cell Signaling Technology, 4060S, 1:1,000 dilution) or rabbit anti-AKT antibody (Cell Signaling Technology 9272S ...
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bioRxiv - Molecular Biology 2020Quote: ... the membranes were washed and incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:3000, Cell Signaling Technology, #7076 or #7074) and followed by detection with ECL Western blot substrate (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: The following primary and secondary antibodies were used for immunoblotting: Anti-EGFR rabbit antibody (no. 4267, Cell Signaling Technology, Danvers, MA, 1:1,000 dilution); anti-phospho-EGFR (Tyr1068 ...
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bioRxiv - Genetics 2021Quote: ... and probed for phosphorylated EIF2α protein levels with rabbit anti-Eif2a S51 phosphorylation polyclonal antibody (1:500 dilution) (Cell Signaling Technologies, cat. number D9G8) and Total EIF2α protein levels with rabbit anti-EIF2a polyclonal antibody (1:10,000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen detection was performed by overnight incubation with appropriate antibodies: 1:1000 rabbit monoclonal Plexin-A4 (catalog #C5D1) anti-mouse (Cell Signaling Technology, catalog #3816), 1:1000 rabbit monoclonal Nrp1 (catalog #D62C6 ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were blocked in 4% FBS in 1x PBS + 0.1% Triton X-100 prior to incubation with primary antibodies diluted in blocking buffer: rabbit anti-γ-H2AX (Cell Signaling, 2577, 1:400); mouse anti-p53 (Cell Signaling ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were blocked in 5% no-fat dry milk in TBST and immunoblotted with rabbit polyclonal anti-CHD7 1:2000 (Cell Signaling Cat. No.6505S), rabbit polyclonal anti-SOX2 1:2000 (Millipore Cat ...
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bioRxiv - Neuroscience 2019Quote: ... Total and phosphorylated glycogen synthase kinase 3 (GSK3-β) were detected using rabbit antibodies anti-GSK3-β (9315) and anti-pGSK3α/β-Ser21/9 (9331) (Cell Signaling). Chemiluminescence signals were analyzed using Image Lab software 6.0 (Bio-Rad).
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bioRxiv - Microbiology 2019Quote: ... Cells were then separately stained for Rab5 or Rab7 by incubating in either rabbit anti-Rab5 (1:100; Cat. 2143S Cell Signaling Technology, Danvers, MA) or rabbit anti-Rab7 (1:100 ...
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bioRxiv - Biophysics 2019Quote: ... chemically fixed cells were permeabilized in 0.1% Triton-X 100 then labeled with anti-human phosphotyrosine CD79A (Tyr182) rabbit antibody (Cell Signaling Technology Cat# 5173, RRID:AB_10694763). The primary antibody was detected using one of two a secondary goat anti rabbit polyclonal antibodies ...
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bioRxiv - Genetics 2019Quote: ... Antibodies used in ChIP are: rabbit monoclonal anti-phospho-Ser22-Lamin A/C antibody D2B2E (Cell Signaling 13448S, Lot 1; 5 µL per IP); mouse monoclonal anti-pan-N-terminal-Lamin A/C antibody E1 (Santa Cruz Biotechnology sc-376248 ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies used included the following: rabbit anti-TCF7L2 (1:1,000 for western blotting and 2 μg for co-IP assays; #2569; Cell Signaling Technology, Danvers, MA, USA), rabbit anti-TCF7 (1:1,000 for western blotting ...
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bioRxiv - Neuroscience 2020Quote: ... and membranes were blotted using antibodies raised against murine haem oxygenase-1 (HO-1; rabbit polyclonal, 1:1000, Cell Signaling Technology, Leiden, The Netherlands) or superoxide dismutase 2 (SOD2 ...
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bioRxiv - Biochemistry 2021Quote: ... or the H2.3 rabbit polyclonal anti-HSulf-2 antibody (dil. 1/500) followed by a horseradish peroxidase-labeled anti-mouse or rabbit IgG antibody (Cell Signaling Technology, Danvers, MA) and ImmunoStar LD (Wako Pure Chemical Industries) ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was washed with 1X TBST (3 × 5 min) and then incubated with respective secondary antibodies (Horse anti-mice 1:2000; Horse anti-rabbit 1:2000, (Cell signaling technology, MA, USA) and developed by using ECL chemiluminescent reagent (Bio-Rad ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were probed with the following antibodies at 1/2500 or 1/5000 dilution: rabbit anti-p44/42 MAPK (ERK1/2) antibody (Cell Signaling, cat # 4695 S) to detect ERK2 ...