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Citations for Cell Signaling Technology :
7851 - 7900 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... and rabbit monoclonal vinculin antibody (clone E1E9V; #13901, 1:1000; epitope: amino terminus of human vinculin protein) were purchased from Cell Signaling Technology.
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bioRxiv - Immunology 2019Quote: ... anti-β-actin antibodies and horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody were purchased from Cell Signaling Technology (Boston, USA). Rabbit anti-claudin1 ...
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bioRxiv - Neuroscience 2019Quote: ... They were then incubated at 4°C overnight with the following primary antibodies diluted in buffer G: rabbit polyclonal anti-cleaved caspase-3 (Asp175) (Cell Signaling, #9661) at 1:250 ...
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bioRxiv - Cell Biology 2019Quote: ... 1:100 for IF) and Rabbit anti-GFP (#2956, 1:100 for IF and 1:1000 for WB, respectively) were from Cell Signaling Technology.
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with the primary antibodies in TBS 0.05% Tween-20 / 5% milk overnight at 4°C and subsequently incubated with HRP coupled secondary goat anti-rabbit (7074; Cell Signaling, Switzerland) and goat anti-mouse antibody (7076 ...
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bioRxiv - Genomics 2020Quote: ... We also validated protein expression and inducibility of the KRABINER rescue transgenes via western blot (mouse anti-myc antibody ThermoFisher #MA1-21316, 1:1000 dilution in 5% milk/TBST; rabbit anti-β-actin loading control Cell Signaling #8457 ...
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bioRxiv - Systems Biology 2020Quote: ... we washed the cells 5 × 5 minutes with 5% BSA-PBS then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 donkey anti-mouse secondary antibody conjugated to Cy3 (Jackson 715-165-150) and 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling #8889). After the secondary incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... Slides were washed 3 times with PBS and incubated in secondary antibody (anti-mouse [Cell Signaling, #4408S]; anti-rabbit [Cell Signaling, #8889S]) for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody was added to each tube (10μL anti-Histone H3, Cell Signaling Technology, cat #4620; 4μg Normal Rabbit IgG, Cell Signaling Technology, cat #2729 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein separation was achieved via SDS-PAGE and proteins were transferred to nitrocellulose for immunoblotting with the following primary antibody dilutions: rabbit monoclonal anti–Caveolin-1 (1:1000; Cell Signaling #3267), mouse monoclonal anti–Actin (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Gapdh was used as a loading control and was detected using a rabbit monoclonal anti-Gapdh antibody (1:2000, Cell Signaling Technology).
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bioRxiv - Bioengineering 2021Quote: ... Membranes were then washed three times in TBST and incubated with horseradish peroxidase-conjugated anti-Rabbit IgG secondary antibody (All antibodies from Cell Signaling Technology) at 1:2000 dilution in TBST containing 5% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in PBS and incubated for 1h with A488-conjugated secondary goat anti-rabbit antibody (1:500, Cell Signaling, USA). Similar procedures were followed for POMC labelling in brain sections from POMC-CreERT2-Ai6 mice ...
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bioRxiv - Immunology 2020Quote: ... Membranes were then washed 3x with TBST at room temperature then incubated with secondary anti-rabbit HRP-conjugated antibody (Cell Signaling Technology) for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mCherry (AB0081; 1:300; SICGEN) for neuronal morphology and anti-phospho-Iϰβ-α (14D4 rabbit monoclonal antibody; 1:500; Cell Signaling) for labeling the AIS ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated in a rabbit anti-HA monoclonal antibody (1:250 in blocking buffer; catalog no. 3724, Cell Signaling, Danvers, MA) for 48 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... Phosphorylated MAPKs were detected using α-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) rabbit monoclonal antibody (1:2000, Cell Signaling Technology, USA) for 1 h at room temperature in a solution of 5 % (w/v ...
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bioRxiv - Zoology 2021Quote: ... followed by sequential incubations at 4°C in one of the primary antibodies (1:1000 in blocking buffer) and an anti-rabbit HRP-conjugated secondary antibody (1:2000 in blocking buffer (Cell Signaling Technology). Visualization of identified total protein and phosphorylated protein bands was completed using an Amershan ECL Plus detection kit (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... Anti-SARS-CoV-2 spike antibody (BEI Resources NR-52947) was used at 1:1000 and anti-rabbit HRP (Cell Signaling Technology) at 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked with 3% BSA or 5% milk and probed with the following primary antibodies-1:1000 rabbit anti-Flag (Cell Signaling technology); 1:100 chicken anti-E.histolytica CSN5 (custom) ...
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bioRxiv - Microbiology 2020Quote: ... Flare Biotech LLC) following by HRP conjugated goat anti-rabbit antibody (1:10,000 Ab in 3 % BSA in TBST; Cell Signaling Technology Inc). Images obtained were analyzed via ImageJ software.
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed three times with TBS-T buffer and incubated at RT for 1 hour with horseradish peroxidase-conjugated secondary anti-mouse or anti-rabbit antibodies (1:5000; Cell Signaling Technology). The membranes were washed three times in TBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit anti-phospho-ERK (Thr202/Tyr204) monoclonal antibody (#4370) and rabbit anti-phospho-Akt (Ser473) monoclonal antibody (#4060) were purchased from Cell Signaling Technologies. Mouse anti-PtdIns(4)P monoclonal antibody (Z-P004 ...
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bioRxiv - Cell Biology 2021Quote: ... the antibody labeling steps were repeated on the same membrane for the rabbit anti-β-Actin antibody (Cell Signaling Technology Cat# 4967). The β-actin antibody was diluted 1:1000 in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed thrice with PBS for 5 min at RT before incubation with the secondary antibody (1:200 rabbit anti-mouse AlexaFluor 488, Cell Signaling Technology) in antibody dilution buffer for 1 hour at RT ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were incubated in TBXT buffer containing 5% non-fat milk and horseradish peroxidase (HRP)-conjugated anti-rabbit or anti-mouse IgG (Cell signaling tech.) and detected by using ECL Western Blotting Substrate (Pierce).
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bioRxiv - Cell Biology 2020Quote: ... Co-immunoprecipitated proteins were eluted from the beads using 150 µl of 1x Laemmli sample buffer for 5 min at +75□C and analysed by western blotting as described above except the HRP-conjugated mouse anti-rabbit native IgG secondary antibody (Cell Signaling, #5127) was used to prevent visualisation of heavy and light chains of co-eluted antibody.
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bioRxiv - Cell Biology 2021Quote: ... the cells were fixed with 4 % paraformaldehyde and 0.2 % picric acid in phosphate buffer (pH 6.9) for 30 minutes at room temperature and incubated with peroxidase-conjugated anti-rabbit antibody (Cell Signaling technology #7074) overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated with the secondary antibody for 1h at room temperature (rabbit-IgG HRP-linked 1:10000, Cell Signaling Technology (#7074), mouse-IgG HRP-linked 1:10000 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were saturated and blots were probed with the following primary antibodies: 1:700 rabbit anti-βArr2 (Cell signaling Cat. No. 3857); 1:1000 rabbit anti-GSK3β (Cell signaling Cat ...
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bioRxiv - Cell Biology 2022Quote: Larval tissues were stained with standard immunohistochemical procedures using rabbit anti-Phospho-JNK polyclonal antibody (Cell Signaling Technology, Cat #4668, 1:100), chicken anti-β-galactosidase antibody (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... cells were washed three times with PBS and incubated with a horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody (Cell Signaling #7074) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... except cells were cross-linked with formaldehyde (1%) for 5 min and cross-linked chromatin immunoprecipitated using an anti-HA rabbit monoclonal antibody (Cell Signaling Technology). Knock-in ESCs were first adapted to serum-free 2i/LIF condition as described before ChIP (15) ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were washed with tris-buffered saline with Tween 20 (TBST) three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S ...
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bioRxiv - Physiology 2022Quote: ... antibody in 0.1% TBST containing 3% BSA overnight at +4°C followed by incubation with secondary HRP-linked anti-rabbit antibody (Cell Signaling, #7074). Immunoblotted proteins were detected with ProSignal Pico ECL Reagent (Genesee Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and horseradish peroxidase-conjugated (HRP) anti-mouse and anti-rabbit secondary antibodies were all obtained from Cell Signaling Technologies (Beverly, MA, USA). Mini-protean precast Tris-Glycine Gels (TGX ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed for 10 minutes thrice with 1×TBST before incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Bioengineering 2022Quote: ... sections were blocked with 3% bovine serum albumin / TBS and then incubated with primary antibodies (rabbit anti α-actinin (Cell Signaling, 3134)) overnight at 4 °C at 1:30 ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies include polyclonal anti-rabbit WDR82 (Cat#99715, 1:1000), H3K4me3 (Cat#9727, 1:1000) and H3 (Cat#9715, 1:2500) from Cell Signaling Technology; hSETD1A from Bethyl Laboratories (Cat#A300-289A ...
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bioRxiv - Cancer Biology 2022Quote: ... Endogenous peroxidase inhibition and blocking with normal horse serum was also carried out before the incubation with primary antibodies (anti-rabbit caspase3, 1:100, Cell signaling #9662), anti-mouse ki67 ...
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bioRxiv - Neuroscience 2021Quote: ... a third set of tissue was incubated in a cocktail of rabbit polyclonal antiserum against cFos (1:1000, Cell Signaling, 2250; AB_2247211) and a mouse monoclonal antiserum against dopamine beta-hydroxylase (DbH ...
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bioRxiv - Neuroscience 2021Quote: ... Two sets of tissue sections from each rat (with each set containing a complete rostrocaudal series of sections spaced by 210 μm) were incubated in a rabbit polyclonal antiserum against cFos (1:10,000; Cell Signaling, 2250; AB_2247211), followed by biotinylated donkey anti-rabbit IgG (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Pathology 2021Quote: ... anti-Rnd3 (3664S), anti-Lamin B1 (12586S), anti-Myc-Tag (2276S), and mouse anti-rabbit IgG (Conformation Specific, 5127S) from Cell Signaling Technology, USA ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... They were rinsed with 1x PBS again and incubated with 50 μL of blocking solution (1x PBS, 0.5% goat serum) with 1:300 anti-rabbit mTOR (Cell Signaling Technology, #2983S) for 1 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of puromycin for 48 hours and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... Transfected cells were selected by culture in the presence of neomycin for 48 hours and then used for HIV-1 replication assays or lysed for immunoblot analysis to measure IRF8 expression using a rabbit monoclonal antibody (Cell Signaling Technology).
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bioRxiv - Microbiology 2020Quote: ... and either used in HIV-1 replication assays or lysed for immunoblot analysis to measure MyD88 and TRIF expression using a rabbit monoclonal antibody to MyD88 (Cell Signaling Technology), a rabbit polyclonal antibody to TRIF (Cell Signaling Technology) ...
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bioRxiv - Developmental Biology 2021Quote: ... A mixture of rabbit anti-pSmad1/5/9 (D5B10) and anti-pSMAD1/5 (41D10) monoclonal antibodies (Cell Signaling, #13820 and #9516, respectively), diluted 1:100 each ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a secondary antibody goat anti-rabbit conjugated to a horseradish peroxidase (HRP) (1:500, 7074S Cell Signaling Technology) during 2 hours at RT followed by a 10 minutes incubation at RT with TSA plus Biotin System (Biotin TSA 1:250 ...