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Citations for Cell Signaling Technology :
7901 - 7950 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with a secondary antibody goat anti-rabbit conjugated to a horseradish peroxidase (HRP) (1:500, 7074S Cell Signaling Technology) during 2 hours at RT followed by a 10 minutes incubation at RT with TSA Biotin System (Biotin TSA 1:100 ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were then incubated overnight at 4 °C with either rabbit anti-p-MEK antibody against Ser217/Ser221 (1:1000) (Cell Signaling, #9121), rabbit anti-p-ERK antibody against Thr202/Tyr204 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... The final concentrations of the primary and secondary antibodies and blocking solutions used in the brain sections are as follows: Rabbit anti-cFos (Cell signaling 2250S), donkey anti-rabbit 647 (Jackson ImmunoResearch 711-605-152 ...
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bioRxiv - Cancer Biology 2019Quote: ... and incubated for 1 hour at 25°C with a solution containing 1/400 goat α-rabbit-Alexa-488 (Cell Signaling 4412S) and 1 μM Hoechst 34580 to detect nuclear material (H21486 Molecular Probes. ...
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bioRxiv - Immunology 2020Quote: ... Following antibodies that were diluted in blocking buffer were used to detect early endosomes and lysosomes at 4C’ overnight: rabbit anti-EEA1 (Clone: C45B10, 3288S, 1:50, Cell Signaling technology), rat anti-LAMP1 (Clone ...
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bioRxiv - Cancer Biology 2020Quote: ... as manufacturer’s recommended protocol following overnight incubation with anti-PAX8 mouse antibody 1:250 (Novus: NBP2-29903) and anti-SOX17 rabbit antibody 1:250 (Cell Signaling: 81778S) at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were incubated with primary antibodies overnight at 40C and followed with the relevant HRP-conjugated secondary antibodies (Anti-rabbit IgG, HRP-linked Antibody cell signaling-7074 and Anti-mouse IgG, HRP-linked Antibody Cell signaling-7076) and visualized using Clarity Western ECL Substrate (BIORAD ...
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bioRxiv - Biophysics 2021Quote: ... membrane and incubated with each antibody (primary antibody) and then with a horseradish peroxidase (HRP)-linked anti-rabbit IgG (secondary antibody; 7076, Cell Signaling Technology). Immunoreactive proteins were detected with Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The secondary antibodies anti-mice IgG horseradish peroxidase (HRP) conjugated antibody and anti-rabbit IgG HRP conjugated antibodies were obtained from Cell Signaling Technology, Inc ...
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bioRxiv - Neuroscience 2022Quote: ... slide mounted sections were incubated overnight at room temperature with a rabbit anti-cFos monoclonal primary antibody (9F6, Cell Signaling, 1:8000), diluted in 0.1M phosphate buffer at pH 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... The inhibition of Akt activity was confirmed by western blot analysis with rabbit antibody against phospho-S473-Akt (#9271, Cell Signaling Technology), rabbit antibody against Akt (pan ...
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bioRxiv - Molecular Biology 2019Quote: ... stained by ponceau for general quality control and immunoprobed with antibodies as following: Aldolase-A (rabbit polyclonal, 1:1000, Cell Signaling Technology), Aly (rabbit polyclonal41 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Immunofluorescent staining was performed as previously described (Ibrahim et al 2019) and the following antibodies were used: rabbit anti-insulin (C27C9, 1:200, Cell Signaling #3014), mouse anti-insulin (L6B10 ...
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Anatomical meniscus construct with zone specific biochemical composition and structural organizationbioRxiv - Bioengineering 2019Quote: ... Samples were then incubated in a mixture of Alexafluor 488-labelled anti-rabbit IgG and Alexafluor 532-labelled anti-mouse IgG (both Cell Signaling Technology) (dilutions ...
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bioRxiv - Systems Biology 2019Quote: ... Membranes were probed with rabbit monoclonal antibodies directed against the diphosphorylated (Thr202/Tyr204) form of ERK1/2 (p42/44 MAPK, Cat. No. 4372, Cell Signaling Technology) and total ERK1/2 (Cat ...
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bioRxiv - Neuroscience 2019Quote: ... the brain sections were treated with 3% H2O2 in Tris-buffered saline and 1% normal goat serum and then incubated in c-Fos 9F6 rabbit antibody (Cell Signaling Technology). The sections were incubated in a Polink-1 horseradish peroxidase detection system for rabbit antibody (GBI Labs ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were then incubated overnight on a plate rocker at 4° C in a 1:200 dilution of anti-phospho-Erk1/2 (Thr202/Tyr204) rabbit monoclonal antibody (Cell Signaling 4370) in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... GRP78) or anti-rabbit-HRP (TMPRSS2) conjugated secondary antibodies at 1:3000 for 2hrs at room temperature (Cell Signaling, Danvers, MA, USA). Visualization of TMPRSS2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Anti-Phospho-p44/42 MAPK (ERK1/2—Thr202/Tyr204, CAT# 9101) and anti-MCL-1 (D35A5 rabbit anti-human primary, CAT# 5453) antibodies were purchased from Cell Signaling Technology. Anti-ELP4 (rabbit anti-human primary ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were then incubated with rabbit anti-α-smooth muscle actin (anti-α-SMA, 1:500, Cell Signaling Technology, Danvers, MA) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit anti-human myosin light chain 2 (D18E2) [with cross-reactivity to mouse] (catalog # 8505, RRID:AB_2728760; Cell Signaling Technology, Danvers, MA, USA), and goat anti-human GAPDH [with cross-reactivity to mouse] (catalog # AF5718 ...
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bioRxiv - Molecular Biology 2020Quote: ... the rabbit monoclonal anti-Actin antibody (12748S, rabbit monoclonal antibody to a synthetic peptide corresponding to residues near the carboxy terminus of human β-actin protein, Cell Signaling) and the rabbit monoclonal anti-acetyl-histone H3 (Lys9 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Vero E6 hTMPRSS2 cells infected with SARS-Cov-2 were lysed and analyzed by Western blot as done in with Rabbit Anti-SARS CoV-2 (COVID-19) Spike (Cat No. GTX135356) (Gene Tex) and Rabbit anti-Vinculin #13901 (E1E9V XP®) (Cell signaling) as loading control ...
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bioRxiv - Cancer Biology 2020Quote: ... DyLight 800 conjugate (1:15000, #5257) and anti-rabbit IgG(H+L) DyLight 680 conjugate (1:15000, #5366) from Cell Signaling Technologies. The images were captured using Odyssey CLx Imager (LICOR Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... 5% BSA (AmericanBio) in TBST (TBS with Tween 20) for 1 h at RT and incubated with AKT (1:1000, rabbit, Cell Signaling 9272), pAKT (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... The vibraslices were then incubated for overnight at RT with rabbit anti-HA antibody (1:1000; Cell Signaling Technology Cat# 3724, RRID:AB_1549585), followed by 1 hr with the Cy5-conjugated goat anti-rabbit IgG ...
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bioRxiv - Genetics 2019Quote: ... Membranes were then washed three times with TBS-T and incubated for 1 h at room temperature with anti-rabbit horseradish-peroxidase-conjugated secondary antibody (Cell Signaling, 7074) diluted 1:20000 ...
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bioRxiv - Physiology 2021Quote: ... Blots were blocked in 5% bovine serum albumin in tris-buffered saline with 0.1% Tween 20 (TBS-T) and incubated overnight at 4°C with rabbit anti-phospo-Ser505-Akt (Cell Signaling Technology 4054) or mouse anti-α-Tubulin (DSHB 12G10 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunohistochemistry was carried out via standard techniques with non-phospho (Active) β-Catenin (#8814, rabbit monoclonal, Cell Signaling Technology; diluted 1:200) and EGR2 (ab43020 ...
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bioRxiv - Developmental Biology 2021Quote: ... incubated overnight at 4°C with a 1:1000 dilution of rabbit anti-phosphorylated-SMAD1/5/8 (clone 41D10, Cell Signaling Technologies) in block ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with 1X PBS and then incubated with secondary Alexa fluor 488 goat anti-rabbit (1:500, Cell Signaling, #4412S) antibody for 1□h ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were then resolved on 10% sodium dodecyl sulfate polyacrylamide gels and blots were probed using the following antibodies: rabbit anti-FLAG (Cell Signaling Technology), rabbit anti-V5 (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... The expression of PSMA protein in each cell line was investigated by western blot using anti-PSMA rabbit antibody (Cell Signaling Technology) [11].
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bioRxiv - Developmental Biology 2021Quote: ... Both Anti-ERVK-env mouse monoclonal (HERM-1811-5, Austral Biologicals, San Ramon, CA, USA) and anti-CDH1 rabbit monoclonal (Cell Signaling, 3195S) antibody were diluted 1:250 in PBS + 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... cells were incubated overnight at 4°C in primary antibody diluted in blocking solution (mouse anti-CamKII from Millipore 05-532, rabbit anti-Gluc from Nanolight 401P, mouse anti-GAD67 from Millipore MAB5406, mouse anti-GFAP from Cell Signaling Tech. 3670 ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated with primary antibodies (0.25% Triton-X, 2.5% normal goat serum, PBS; 24h) against Fos (1:800 rabbit anti-cFos, Cell Signaling #2250; RRID: AB_2247211) or tyrosine hydroxylase (1:400 mouse anti-TH ...
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bioRxiv - Developmental Biology 2021Quote: ... and Samples were washed extensively in PBS-Tx (6 × 1 hour) and incubated with anti-rabbit-HRP (Cell Signaling #7074, 1:1000) diluted in 1% BSA/PBS-Tx ...
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bioRxiv - Systems Biology 2021Quote: ... we washed the cells 5 X 5 min with 5% BSA-PBS and then incubated the cells at room temperature for 1 hour in 5% BSA-PBS containing 1:500 goat anti-rabbit secondary antibody conjugated to Alexa Fluor 594 (Cell Signaling, 8889). After the secondary incubation ...
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bioRxiv - Neuroscience 2021Quote: ... Immunofluorescence staining was performed by a standard protocol using an anti-HA rabbit primary antibody (C29F4, Cell Signaling Technology, diluted 1:1600) and a secondary antibody goat anti-rabbit Alexa Fluor 633 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... Specific proteins were detected using 1:1000 dilution of Myc-Tag (9B11) mouse monoclonal and proliferating cell nuclear antigen (PCNA, D3H8P) XP Rabbit monoclonal primary antibodies (Cell Signaling Technology). Following primary antibody incubation ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the following antibodies were used anti-Bassoon (1:400, SAP7F407; mouse monoclonal; Enzo Life) and anti α-synuclein (1:500, D37A6; rabbit polyclonal; Cell Signaling). Species-specific secondary antibodies coupled to Alexa 488 and 350 (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... MAPK activity was assessed using phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) primary antibody (1:200, rabbit, Cell Signaling Technologies #9101), total p44/42 MAPK (ERK1/2 ...
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bioRxiv - Pathology 2021Quote: ... The separated proteins were transferred onto a nitrocellulose membrane for detection with polyclonal rabbit anti-Phospho-Myosin Light Chain 2 (Ser19) antibody (Cell Signaling Technologies) or monoclonal rabbit anti-Myosin Light Chain 2 (D18E2 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were immunoprecipitated from precleared lysates overnight at 4°C using 4 μg of control IgG (rabbit or mouse, Cell Signaling Technology) or indicated antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... ERK1/2 (#9102, Rabbit, WB 1:1000) and MEK1/2 (#4694, Mouse, WB 1:1000) antibodies were purchased from Cell Signaling Technology, MA ...
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bioRxiv - Cell Biology 2020Quote: ... and two different YAP1 antibodies were used to characterize YAP1 expression patterns: mouse monoclonal anti-YAP1 antibody (catalog # sc-101199,1:50) and rabbit monoclonal anti-YAP1 antibody (catalog # 14074S, Cell Signaling,1:100). To prevent nonspecific binding of the antibodies ...
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated at 4°C overnight with the following primary antibodies: rabbit anti-ubiquitin (1:1000; Cell Signaling Technology no. 3933), rabbit anti-phospho-eIF2α (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... To detect phosphorylated ERM proteins a rabbit anti-phospho–ezrin (Thr567)/radixin (Thr564)/moesin (Thr558) (pERM) antibody was used (Cell Signaling Technology). Isotype control staining was performed using rabbit IgG isotype mAb Alexa Fluor 647 (DA1E ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were labelled overnight with primary antibodies diluted in PBS 10% (w/v) BSA (for anti-RelA: rabbit monoclonal, Cell Signaling Technology #8242 ...