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Citations for Cell Signaling Technology :
7801 - 7850 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-phospho phospholamban (Ser16/Thr17) antibody (#8496) and rabbit anti-phospho troponin I (Ser23/24) antibody (#4004) are from Cell Signaling. Rabbit anti-phospho ryanodine receptor 2 (Ser2808 ...
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bioRxiv - Neuroscience 2023Quote: ... and corresponding polyvinylidene difluoride (PVDF) membranes were incubated overnight at 4°C with various antibodies: rabbit anti-Bcl-2 (1:500; #3498, Cell Signaling), rabbit anti-Bax (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... each membrane was probed with the appropriate horseradish peroxidase-conjugated secondary antibody (anti-mouse dilution 1:5,000; GTX213112-01, Gene Tex; anti-rabbit dilution 1:3000; 7074S, Cell Signaling Technology), and incubated for 1□h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody was either goat anti-rabbit (7074) or horse anti-mouse (7076) IgG conjugated with horseradish peroxidase (Cell Signaling Technology). Staining was developed with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific ...
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bioRxiv - Immunology 2023Quote: ... slides were incubated with one or several of the following primary antibody / lectin: 1:300 rabbit anti-ZAP70 monoclonal antibody (99F2 – Cell Signaling), 1:40 cytokeratin Pan Type I/II mouse monoclonal antibody cocktail (Thermofisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies used in this study were 1:2,500-diluted mouse anti-β-actin (Wako, #017-24551) and 1:1,000-diluted rabbit anti-cleaved caspase-3 (Cell Signaling, #9661). The membrane was washed three times with PBS for 10 minutes and incubated with secondary antibodies at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Western blot analysis was carried out as described (60) using antibodies specific for rabbit EZH2 (Cell signaling technology, #5246, 1:1000), rabbit Amylase (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed two times with PBS and incubated with 50 µL of primary antibody (α-LAMP1 rabbit, Cell Signaling, 1:100 in 1 % BSA ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were labeled with mouse anti-GFP (1:500) antibody and rabbit anti-FOXP1 antibody (Cell Signaling Technology; 1:400) and detected by biotinylated secondary antibody/streptavidin-Alexa Fluor 488 (GFP ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The membrane was shaken three times for 5 min in TBST then incubated with 1:3,000 anti-rabbit HRP-linked antibody (Cell Signaling Technologies) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated overnight at 4°C with the primary c-Fos antibody (1:1000, Rabbit, Cell Signaling #2250). After 3 rinses with PBS for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... The commercial antibodies used in this study included a GFP (D5.1) XP Rabbit monoclonal antibody (Cell Signaling Technology, Danvers, MA, USA), GAPDH (glyceraldehyde-3-phosphate dehydrogenase ...
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bioRxiv - Cell Biology 2023Quote: ... goat anti-Choline Acetyltransferase (ChAT) (1:500, MilliporeSigma, AB144P, RRID:AB_2079751) and rabbit anti-DARPP-32 (19A3) (1:400, Cell Signaling, 2306, RRID:AB_823479). All secondary antibodies used in this study were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Biochemistry 2023Quote: ... Overexpression of Flag-NUDT15 and HA-NUDT18 were confirmed by analyzing cell lysates by SDS-PAGE followed by Western blotting using anti-FLAG-tag Rabbit antibody (#740001, Thermo Fischer Scientific) and anti-HA-tag (6E2) Mouse antibody (#2367, Cell Signaling) followed by anti-Rabbit HRP or anti-Mouse HRP ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by incubation with primary antibodies at 4 °C overnight (the primary antibodies were rabbit anti-GAPDH antibody, 1:2000, Cell Signaling, Cat#5174 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed 5X with cold TTBS and incubated with anti-Rabbit or anti-mouse secondary antibodies conjugated to HRP (#7074 and #7076, Cell Signaling). After washing ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with a respective secondary HRP-conjugated antibody (anti-rabbit: Cell Signaling Technologies, #7074S, anti-mouse: Cell Signaling Technologies, #7076) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was blocked in 5% dry milk in TBS-T for 30 min and incubated overnight at 4°C with primary antibodies against: rabbit V5 at 1:1,000 (Cell Signaling Technology), mouse vinculin at 1:1,000 (Millipore Sigma) ...
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bioRxiv - Physiology 2024Quote: Mouse retinae were fixed in 4% formaldehyde in PBS for 1h and wholemount immunostained as described [31] using rabbit anti-P-p38 MAPK (Thr180/Tyr182) antibody (Cell Signaling) followed by Alexa Fluor 555–conjugated goat anti–rabbit antibodies (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated with horseradish peroxidase-conjugated secondary IgGs (1:1,000 for rabbit #170-6515, mouse #170-6516, Bio-Rad, CA or 1:2,000 for #7076s, Cell signaling, MA), for 1hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were visualized after staining with the appropriate goat anti-rabbit conjugated to Alexa-488 (1:500, Cell Signaling, 4412) and and donkey anti-chicken IgG conjugated to Alexa-647 (1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 2 h blocking with 15% milk powder (in TBST) and overnight incubation with rabbit anti-BIP (3183, Cell signaling, 1/1000 for retina ...
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibodies used in this study were as follows: rabbit anti-DNMT3B (1:2000; 57868, Cell Signaling Technology, Massachusetts, USA), rabbit anti-PLCG2 (1:2000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies used were anti-rabbit and anti-mouse IgG HRP-linked antibodies (1:5000, Cell Signaling, 7074 and 7076 respectively). Images were developed using the MINI-MED 90 X-Ray Film Processor (AFP Manufacturing Corp) ...
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bioRxiv - Immunology 2024Quote: ... membranes were treated with stripping buffer for 30 minutes and subsequently re-probed with a rabbit anti-GAPDH primary antibody (14C10; 1:20,000 dilution; Cell Signaling Technology) followed by the same HRP-conjugated secondary antibody ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times and incubated with anti-mouse IgG or anti-rabbit IgG HRP-linked antibodies (Cell Signaling Technology) both diluted in PBST (1:2,000) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The plates were incubated overnight at 4°C with rabbit anti-HA antibody (1:1000, catalog #3724S, Cell Signaling, Danvers, MA) diluted in 5% bovine serum albumin ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were eluted by heating Protein-G agarose beads and anti-HA magnetic beads for 15 min at 42°C in 50μL 2X sample buffer and analyzed by western blotting using rabbit anti-HA (1:1000, Cell Signaling Technology) and mouse anti-FLAG (1:1000 ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Membranes were then incubated with rabbit monoclonal anti-CtIP antibodies (1:3000 dilution in TBS-T; #9201, Cell Signaling Technology, USA), rabbit monoclonal anti-cleaved caspase-3 (1:3000 dilution in TBS-T ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were next incubated overnight at 4 °C with Shh (C9C5) rabbit antibody (1:500, Cell Signaling Technologies, Cat. No. 2207) in PBS-BSA-T ...
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bioRxiv - Molecular Biology 2024Quote: ... The following primary antibodies and dilutions were used in this study: rabbit anti-G9a/EHMT2 (C6H3) (Cell Signaling, 3306T, 1:200), anti-histone H3K9me3 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... the blots were washed in TBS-0.05% tween-20 and incubated with a secondary antibody HRP-conjugated Goat anti-Rabbit IgG (Cell Signaling Technology). The anti-actin antibody was directly conjugated to HRP ...
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bioRxiv - Microbiology 2024Quote: ... Parasites were stained overnight with 13.1 mice monoclonal anti-P28 (1:1000) and rabbit anti-HA (1:250, Cell Signaling Technologies) antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... membranes were washed three times for five minutes with TBS-T and incubated for one hour with 1:15,000 HRP-linked anti-rabbit or anti-mouse antibodies (Cell Signaling, 7074S, 7076S) or 1:15,000 DyLight anti-rabbit IgG 800 conjugate (Cell Signaling ...
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bioRxiv - Molecular Biology 2020Quote: ... Blots were washed three times (total 30 min) and incubated with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Cell Signaling Technologies) for 1 h at room temperature with gentle rocking ...
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bioRxiv - Cell Biology 2020Quote: ... primary rabbit polyclonal antibodies against cleaved caspases-3 and light chain 3 (LC3) were procured from Cell Signaling Technology (Danvers, MA, USA). APG-5 ...
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bioRxiv - Cell Biology 2019Quote: ... 1D4, DSHB), mouse anti-Fasciclin III (1:100; 7G10, DSHB) and rabbit anti-phospho-JNK/SAPK (1:100; 81E11 Cell Signaling Technology). Appropriate secondary antibodies conjugated either with Cy3 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... The Developmental Studies Hybridoma Bank at the University of Iowa) and Rabbit anti-cleaved Drosophila Dcp-1 (Asp216) (1:100; Cell Signaling Technologies). Secondary antibodies were used at a dilution of 1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... for 1 hour and probed overnight at 4°C with the primary antibodies of interest directed against: rabbit anti-BCL-2 (Cell Signaling, CST4223), rabbit anti-BCL-xL (Cell Signaling ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with the following primary antibodies for 1 hour at RT: rabbit anti-PPAR-gamma 1:200 (Cell Signaling 2443S), mouse anti-SMA 1:300 (Sigma A5228 ...
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bioRxiv - Genetics 2021Quote: ... Membranes were washed in TBS-T and incubated with HRP-conjugated anti-goat IgG (R&D, HAF017) or anti-rabbit IgG (Cell Signaling, 7074S) antibody in Blotto A (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.3% Triton X-100), incubated in rabbit anti-phospho-Histone H3-S10 at 1:2000 overnight (O/N, 4°C) (Cell Signaling 3377S), washed 8X in PBSTx (1X PBS plus 0.3% Triton X-100 ...
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bioRxiv - Pathology 2022Quote: ... The blotted membranes were incubated with the following primary antibodies: rabbit monoclonal anti-phosphorylated Smad2 (Ser465/467) antibody (Cell Signaling Technology, #3108), rabbit monoclonal anti-Smad2 antibody (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for immuno-blotting included rabbit monoclonals directed against Mlx and Myc (#85570 and #13987, Cell Signaling Technologies, Inc., Danvers, MA) and a mouse monoclonal antibody against GAPDH (#G8795 ...
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bioRxiv - Cancer Biology 2022Quote: ... in TBST and then probed with listed antibodies diluted in either 1% BSA (anti-phospho-specific antibodies; MPI) or milk and anti-rabbit-HRP or anti-mouse-HRP (Cell Signaling Technology) where appropriate.
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bioRxiv - Neuroscience 2021Quote: Antibodies on these sections included Rat anti-CD3 epsilon (1:100, NBP1-26685, Novus Bio) and Rabbit anti-C-Kit (1:100, D13A2, Cell Signaling Tech).
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bioRxiv - Cell Biology 2022Quote: ... Primary and secondary antibody incubations were done in 5% milk/PBS using rabbit anti-HA at 1:500 (Cell Signaling, Cat #: 3724S) and anti-Rabbit HRP at 1:10,000 (BioRad ...
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bioRxiv - Neuroscience 2019Quote: ... Cells then underwent immunocytochemical processing similar to that described above using primary antibodies against activated c-Jun-N-terminal kinase (rabbit anti-pJNK, 1:100, Cell Signaling Technology) or phosphorylated p38 MAPK (rabbit anti-p-p38 ...
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bioRxiv - Neuroscience 2019Quote: ... and then incubated for 24 hours in solutions containing either HA-tag antibodies (raised in rabbit, clone C29F4, 1:400, cat # 3724, Cell Signaling Technology, Danvers ...
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bioRxiv - Cancer Biology 2019Quote: ... the membranes were washed four times with TBST (5 minutes gentle rocking per wash) and incubated overnight with primary antibody Rabbit anti-Gli-1 (Cell signaling, #C68H3); 1:250 dilution in TBST-Milk at 4°C with gentle rocking ...