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Citations for Cell Signaling Technology :
7701 - 7750 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... followed by incubation with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies (1:5000, Cell Signaling Technology, Danvers, MA, USA). The samples were normalized with GAPDH (1:5000 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Anti-rabbit IgG (H+L)-F(ab’)2 Fragment-Alexa Fluor 555 Conjugate (cat. No. 4413, Cell Signaling Technology, Beverly, USA), CRLS1 pAb (cat ...
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bioRxiv - Pathology 2020Quote: ... for one hour at 25°C and incubated overnight with the rabbit anti-phospho-MEK1/2 (Ser217/221) antibody (Catalog #9154; Cell Signaling), the mouse anti-MEK1/2 antibody (Catalog #4694 ...
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bioRxiv - Immunology 2020Quote: ... The membranes were then washed and incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibodies from Cell Signaling Technology and GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... and then incubated for 1 h with 1:1,000 dilution of secondary HRP-conjugated goat anti-rabbit IgG antibody (Cell Signaling Technology). Images of the immunoblots were developed with ECLTM Western Blotting Detection Reagent (GE Healthcare).
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bioRxiv - Developmental Biology 2021Quote: ... and 0.05% Tween 20] plus 5% BSA before incubation with the primary antibody (1:1000 for mouse monoclonal 12C11 anti-REST and 1:2000 for anti-LaminB1 rabbit polyclonal, Cell Signaling). Anti-rabbit IgG or anti-mouse IgG conjugated to horseradish peroxidase (1:2000 ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were then washed and incubated with either mouse or rabbit secondary antibody linked with horseradish peroxidase (Cell Signaling Technologies) and washed ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated goat anti-mouse (1:4000) and goat anti-rabbit (1:4000) secondary antibodies were obtained from Cell Signaling Technology, Danvers ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit polyclonal anti-phospho-myosin light chain 2 (Thr18/Ser19) (for immunostainings on elastomers; 1:200, Cell Signaling; cat. no. #3674). An anti-Plexin-B1 antibody was raised against the extracellular domain of Plexin-B1 ...
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bioRxiv - Cell Biology 2021Quote: ... Phospho-Akt and histone H3 were detected by incubation with rabbit anti-phospho-Akt (Cell Signaling Technologies, #4054, diluted 1:1000) and rabbit anti-histone-H3 (Abcam #1791 ...
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bioRxiv - Cancer Biology 2021Quote: ... and then probed for one hour at room temperature with a 1:3000 dilution of anti-rabbit (Cell Signaling Technology, #7074) IgG HRP-linked antibody and developed using Pierce ECL Western Blotting Substrate (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... The membranes were blocked with 5% non-fat dry milk for 2 h at RT and incubated overnight at 4°C with rabbit anti-phospho-ERK1/2 (1:2000, Cell Signaling), anti-phospho-Ser473-Akt (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were washed with PBS-T and incubated with HRP-coupled goat anti-mouse or anti-rabbit IgG antibodies (Cell Signaling). Detection was performed with Clarity Lightning ECL (Bio Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Thermo Fischer Scientific MA5-11690) overnight at 4°C and then incubated with goat anti-rabbit HRP linked IgG (1:10000, Cell signaling). Membranes were visualized using a ChemiDoc MP (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... The blots were subsequently reblocked and probed with a rabbit monoclonal anti-GAPDH antibody (#2118; 1:5000, Cell Signaling Technology Inc.). The ratios of D3R/GAPDH ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... MCF7 cell lysate as prepared above was diluted to 0.8 mg/mL total protein in 1:3 lysis buffer:dilution buffer with 0.5 nM rabbit anti-BRD4 antibody (Cell Signaling Technology; E2A7X) and 1 nM CoraFluor-1-labeled anti-rabbit nano secondary ...
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bioRxiv - Cancer Biology 2020Quote: ... Human-specific EpCam-647 (clone VU1D9, #5447) and rabbit anti-human γH2AX antibodies (Phospho-Histone H2A.X Ser139; clone 20E3, #9718) from Cell Signaling Technologies, and EpCam-APC antibody (clone EBA-1 ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C and secondary HRP-conjugated anti-rabbit IgG (1:2,000 in 5% nonfat dry milk; Cell Signaling Technology) for 1 h at RT ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed with PBST for 30 min before incubating with either the fluorescently conjugated secondary antibodies (1:20000) (Li-COR) or horseradish-peroxidase conjugated goat anti-rabbit secondary antibody for the Nrf2 blot (1:5000, #7074) (Cell Signaling) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated overnight in rabbit anti-c-Fos primary antibody (1:200 in blocking solution, Cell Signaling Technologies, Ab #2250). Following overnight incubation in primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with the primary antibodies rabbit anti-phospho-β catenin (Ser675, 1:100, D2F1, Cell Signaling Technology, MA, USA) and mouse anti-β-catenin (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The membranes were then probed with goat anti-rabbit and goat anti-mouse secondary antibodies (#7074, #7076, Cell Signaling, Danvers, MA), and blots were incubated with Clarity Western ECL Substrate (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... 10% normal donkey serum in 1xPBS) and then incubated overnight at 4°C with a rabbit polyclonal antiserum against cFos (1:1000, Millipore and Cell Signaling) followed by a Cy3-conjugated donkey-anti-rabbit secondary antibody (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Rabbit anti-Integrin β1 was a gift from the Albiges-Rizo’s lab. Rabbit monoclonal anti-Integrin β5 (ref. 3629) was purchased from Cell Signaling Technology. Mouse monoclonal anti-integrin αvβ5 (ref ...
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bioRxiv - Cell Biology 2022Quote: ... We also used the following commercial antibodies for western blotting and/or immunofluorescence staining: rabbit anti-WASF3 (#2806; Cell Signaling Technology), mouse anti-ARP3 (ab49671 ...
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bioRxiv - Developmental Biology 2022Quote: ... The antibodies for western blotting were rabbit anti-COFILIN monoclonal antibody (1:1,000 dilution, 5175, Cell Signaling Technology, Danvers, MA, USA), rabbit anti-phospho-COFILIN monoclonal antibody (1:1,000 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... and 1:5000 dilution of HRP-conjugated rabbit anti-mouse secondary antibody (Cat. No. 7076, Lot No. 36, Cell Signaling Technology), and the blot was developed similarly.
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bioRxiv - Cancer Biology 2022Quote: ... 2021) using 25μg of chromatin and 10ug of either anti-HIF1α ChIP antibody (Novus) or anti-rabbit IgG (Cell Signaling Technologies).Chromatin was immunoprecipitated overnight at 4C and HIF1α binding sites assayed via RT-qPCR using 2μl of either input ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies used in this study were as follows: rabbit monoclonal active β-catenin (Cell Signaling, 19807S, 1:100 with TSA), mouse monoclonal ...
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bioRxiv - Immunology 2022Quote: ... NFκB phospho (rabbit-anti-NFκB p65 total, Cell Signaling Technology #8242; mouse anti-NFκB p65 p-Ser536, Cell Signaling Technology #13346), mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT ...
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bioRxiv - Immunology 2022Quote: ... The membranes were blocked with 5% skim milk and then treated with Trim28 primary and anti–rabbit IgG (Cell Signaling Technology) secondary antibodies ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and rabbit antibodies specific for p-Erk1/2 (phospho-p44/42 MAPK) (1:1000, #4370; Cell Signaling Technology, Beverly, MA, USA), Erk1/2 (p44/42 MAPK ...
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bioRxiv - Pathology 2022Quote: ... blots were transferred to a solution containing anti-rabbit IgG HRP-linked secondary antibody (1:1000 dilution; Cell Signaling Technology 7074S) and incubated for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated overnight at 4°C with rabbit anti-phospho-acetyl CoA carboxylase (Ser79) antibody (#3661; Cell Signaling Technology Europe BV ...
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bioRxiv - Neuroscience 2022Quote: ... The plates were washed 3x with PBST and incubated overnight at 4°C under agitation with rabbit anti-phosphorylated TRKB (Cell Signaling, either #4168 against pY816 or #4619 against pY515 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated in the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies (FosB anti-rabbit HRP, GAPDH anti-mouse HRP, Cell signaling) at 1:500 for 1 hr at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubated with the primary antibodies: polyclonal rabbit antibody against PTP1B (2 μg/mL; #5311; Cell Signaling Technology, Boston, MA, USA) and monoclonal mouse antibody against Cdk3 (2 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The sections were incubated with the following primary antibodies: rabbit monoclonal anti-phosphorylated Smad2 antibody (Ser465/467) (Cell Signaling Technology, #3108), rabbit polyclonal anti-phosphorylated ERK1/2 antibody (Thr202/Tyr 204 ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were then incubated for 60-180 min at RT with HA antibody (rabbit, 1:250, Cell signaling Technology, cat #C29F4) in PBS–GS blocking solution ...
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bioRxiv - Physiology 2022Quote: ... and probed with rabbit anti-c-Src primary antibodies raised against c-Src in its non-phosphorylated (1:2,000; Cell Signaling, UK) or phosphorylated form (1:1,000 ...
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bioRxiv - Microbiology 2024Quote: ... They were passed through a 0.45 µm filter and precleared with 2 μg/ml anti-rabbit isotype IgG antibody (Cell Signaling #2729) with protein A/G magnetic beads (Pierce ...
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bioRxiv - Microbiology 2024Quote: ... The following antibodies were used for immunohistochemistry (IHC) staining: rabbit anti-CD8 alpha (cytotoxic T cell) (#98941S, Cell Signaling Technology, USA) (1:250 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... FOS immunostaining was conducted using rabbit anti-phospho-FOS monoclonal antibody (1:1000, Cat # 5348, RRID:AB_10557109; Cell Signaling Technology, MA, U.S.A.). Slices were washed in PBS 6 × 10 min each ...
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bioRxiv - Neuroscience 2022Quote: ... Targets were detected after incubation with a goat anti-rabbit secondary antibody horseradish peroxidase conjugated (1:10,000; Cell Signaling, Cat# 7074P2) for 1 hour and visualized with the chemiluminescence reagent (Immobilon Forte Western HRP Substrate ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: Human CDH5 and ICAM-1 protein levels were evaluated by SDS PAGE using rabbit anti-CDH5 (Cell signaling technology Cat# 2500) and mouse anti-ICAM-1 (Santa Cruz biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... and HRP-linked anti-Rabbit IgG and HRP-linked anti-Mouse IgG (Cell Signaling, cat# 7074 and 7076; WB 1:1000). IF – immunofluorescence ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... membranes were incubated with the following primary antibodies in the blocking buffer: rabbit anti-GAPDH (1:2,500; Cell Signaling Technology #2118) and mouse anti-HiBiT (1.0 µg/ml ...
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bioRxiv - Physiology 2022Quote: ... GAPDH-HRP (GTX627408-01, 1:120000) and P84 (GTX70220-01, 1:1000) The secondary antibodies were Anti-rabbit IgG (Cell Signaling, 7074 ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies and concentrations were utilized: rabbit anti-Vimentin (Cell Signaling Technology, D21H3, WB 1:1000, IF 1:200), mouse anti-Vimentin (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... cells were incubated overnight at 40C with one or more of the following antibodies: rabbit anti-pMLC (ser19) (Cat# 3675, Cell Signaling), rabbit anti-paxillin (Cat# PA534910 ...