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Citations for Cell Signaling Technology :
7651 - 7700 of 8370 citations for UBE2I Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and chemiluminescence protein detection was done as described with anti-mouse or anti-rabbit antibodies conjugated with horseradish peroxidase (HRP) from Cell Signaling Corp ...
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bioRxiv - Systems Biology 2020Quote: ... Membranes were then incubated for one hour at RT in either appropriate (mouse or rabbit) Horse radish peroxidase (HRP) - linked secondary antibodies (Cell Signaling) in TBST for chemiluminescent detection ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were incubated overnight at 4°C with the following antibodies: rabbit anti-phospho-Akt (Ser473) (1:1000; clone D9E, Cell Signaling), rabbit anti-Akt (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... They were then incubated overnight at 4°C with the following primary antibodies diluted in PBS - 0.3% Triton X100 - 1% normal serum: rabbit anti-cleaved caspase 3 (1/400, Cell Signaling, 9661), rabbit anti-doublecortin (1/2000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit anti-Tubulin (cat# 2148), rabbit anti-NKX2.5 (clone E17AH, cat# 8792S), and mouse anti-SOX2 (clone L1D682, cat# 4900S) were from Cell Signaling Technologies. Mouse anti-OCT4 (cat# ab184665) ...
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bioRxiv - Neuroscience 2019Quote: ... and/or immunoblotting (IB) at the indicated dilutions: Rabbit anti-peIF2a (for PLA 1:6000 #44728G Invitrogen, and IB, 1:1000, Cell Signaling), mouse anti-eIF2a (IB ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then washed three times with TBS-T followed by incubation during 1 hr at room temperature with anti-rabbit or anti-mouse secondary antibody coupled to HRP (all from Cell signaling) diluted at 1:1,000 in blocking buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... blocked and incubated with an antibody recognizing Myosin Light Chain 2 phosphorylated at serine19 (1:200, rabbit; Cell signaling technology, #3671), or with the anti-tyrosinated tubulin antibody YL1/2 (1:50 ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed in PBS-0,1% Tween20 three times for 15 min at room temperature and anti-rabbit or anti-mouse secondary antibodies (1:3000, Cell signaling) were applied for 30 min at room temperature with gentle agitation ...
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bioRxiv - Cell Biology 2020Quote: Protein expression levels of PANX1 were examined by western blotting using a rabbit monoclonal antibody against human PANX1 (D9M1C, Cell Signaling). Actin was also detected as a control by western blotting using a mouse monoclonal anti-β-actin antibody (Santa Cruz) ...
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bioRxiv - Cancer Biology 2021Quote: ... Membranes were blocked in 5% milk protein and incubated with primary antibodies (Table S3) overnight followed by secondary HRP-linked goat anti-rabbit and anti-mouse (Cell Signaling) antibodies (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were then incubated for 30 min with a horseradish peroxidase-linked secondary antibody (anti-rabbit or antimouse IgG, Cell Signaling). The Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were then incubated for 48 hr at 4 °C with diluted primary antibody for c-fos (9F6) (anti-rabbit, 1:1000; Cell Signaling). Third ...
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bioRxiv - Cancer Biology 2022Quote: ... (ZYTOMED, ZYT-ZUC050-006) and ZytoChem Plus (AP) Polymer anti-rabbit, (ZYTOMED, ZYT-ZUC031-006) diluted in antibody diluent (Cell Signaling) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: Human cancer tissue array staining was subjected to antigen retrieval at 98 °C for 20 min in EDTA buffer (pH 9.0) in a steamer and incubated with a rabbit anti-cleaved caspase 3 antibody (Cell Signaling, 9664L) 1:150 overnight at 4 °C followed by anti-rabbit 594 (Invitrogen A11012 ...
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bioRxiv - Cancer Biology 2022Quote: ... rinsed in phosphate buffer 0.2 % Triton for 15 min and incubated with rabbit anti-P-cad (1:50; Cell Signaling; 2130S) or anti-pSrc (pY419 ...
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bioRxiv - Neuroscience 2022Quote: ... the following primary antibodies were used: rabbit anti-phospho-Akt (Ser473; Cell Signaling Technology, catalog no. 4060, 1:1000 for WB), mouse anti-Akt (Cell Signaling ...
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bioRxiv - Immunology 2022Quote: ... The optimized detection panel includes antibody-OPAL fluorophore pairs in the following order: Monoclonal Rabbit Anti-Mouse F4/80 (AB_2799771; clone D2S9R; cat. no. 70076s, Cell Signaling Technology) with OPAL 520 ...
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bioRxiv - Developmental Biology 2022Quote: ... Discs were incubated with primary antibodies overnight at 4°C: rabbit anti-Cleaved Drosophila cDcp-1 (1:200, Cell Signaling, #9578S), rabbit anti-fkh (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were incubated in PBS-T containing 3% BSA (PBS-T-BSA) for 30 min and then incubated overnight at 4 °C with rabbit polyclonal anti-RPS6 antibody (2211, Cell Signaling), rabbit polyclonal anti-MRPS27 antibody (17280-AP ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with the following primary antibodies overnight: rabbit anti-RAD18 NB100-61063 (Novus) (1:200) and mouse anti-biotin (D5A7) (1:200) (Cell signaling). The rest of the protocol follows Proximity Ligation Assay as above.
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bioRxiv - Neuroscience 2020Quote: ... One equal volume was kept for total extracts and the rest was incubated with antibody against mGlu5 (rabbit clone D6E7B from Cell Signaling) with rotation overnight at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... and further incubated with the secondary antibody (1:5,000 anti-rat [7077S]; anti-rabbit [7074S]; anti-mouse [7076S] (Cell Signaling Technology)) conjugated to horseradish peroxidase followed by ECL (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... followed by the addition of goat anti-rabbit IgG and goat anti-mouse IgG HRP-conjugated antibodies (Cell signaling; 1:1000). The protein bands were visualized by the addition of Super signal West Pico Plus chemiluminescence substrate (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... samples were incubated 1 hour at room temperature with primary rabbit anti-phospho Stat1 (Y701, Cell Signaling Technology #9167, clone 58D6), then washed and stained 1 hour with Alexa Fluor 647-conjugated anti-rabbit secondary antibody (Cell Signaling Technology #4414) ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were incubated with a combination of rabbit polyclonal antibodies against phospho-M-CSF receptor (Tyr546, Tyr708, Tyr723 and Tyr923, Cell signaling), phospho-AKT (Ser473 ...
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bioRxiv - Molecular Biology 2020Quote: ... Briefly, sections were incubated with primary antibody (rabbit anit-PH3, 1:100 dilution in immunofluorescence buffer,) (Cell Signaling, Danvers, MA, 9701S) overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:400 ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated overnight at 4°C with the following primary antibody: rabbit anti-IKKα (Cell Signaling 2682; 1:1,000), rabbit anti-phospho-IKKα/β (Ser180/Ser181 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed with TBST prior to incubation for 2 hrs at room temperature in the appropriate secondary antibody (anti-rabbit or anti-mouse; Cell Signaling) at 1:2000 dilutions ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed three times with TBS containing 0.1% Tween 20 then incubated with anti-Rabbit IgG-HRP conjugated (Cell Signaling: 7074). Images were acquired by chemiluminescence using Clarity ECL (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-chicken 488 (Life Technology A11039 at 1/200) and goat anti-rabbit 488 (Cell Signaling Technology #4412 1/200).
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-HA tag (C29F4) monoclonal antibody (1:1000 for NaV1.6 and 1:2000 for NF186; Cell Signaling, cat. no. 3724); mouse anti-ankyrin G antibody (1:50 ...
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bioRxiv - Pathology 2022Quote: ... Then membranes were incubated with peroxidase-conjugated secondary antibodies for 1 h at room temperature: anti-rabbit immunoglobulin G (IgG) (AB_2099233; Cat.#7074, 1:2000, Cell Signaling Technology), anti-mouse IgG (AB_330924 ...
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bioRxiv - Immunology 2022Quote: ... Immunohistochemistry staining using the Monoclonal Rabbit Anti-Mouse CD45 (AB_2799780; clone D3F8Q, cat. no. 70257S, Cell Signaling Technology, Danvers, MA, USA) was performed using the Leica Bond Max Automatic Staining System in a peroxidase-mediated oxidation of 3,30-diaminobenzidine (DAB ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed twice with PBST and incubated with HRP conjugated anti-rabbit IgG (1:500, catalog 7074S, Cell Signaling Technology) for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: Larval wing imaginal discs were stained using the following antibodies: Rabbit anti-cleaved Drosophila Dcp-1 (1:300, Cell Signaling Technology), Rabbit-anti-p-eIF2α (1:300 ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit immunoglobulin G (IgG) (H + L) (Dylight 800 conjugates) and anti-mouse IgG (H + L) (Dylight 680 conjugates) (Cell Signaling) were used as secondary antibodies.
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bioRxiv - Microbiology 2022Quote: ... The secondary antibodies used for immunoblotting were anti-rabbit IgG (H+L) (DyLight™ 800 4X PEG Conjugate) (Cell Signaling, 5151S) and anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were fixed in ice-cold 70% ethanol and stained with propidium iodide (50 μg/mL)/RNase (100 μg/mL) and rabbit-anti-phospho-HistoneH3-antibody (#9701, Cell Signaling) and goat-anti-rabbit-Alexa-488-conjugated secondary antibody (#R37116 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were incubated overnight at room temperature (1/100 rat anti-BrdU OBT0030 AbD Serotec; 1/400 rabbit anti-Caspase #9661 Cell Signaling; 1/250 rabbit anti-OTX2 ab92326 Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were the monoclonal anti-PD-L1 antibody raised in rabbit (1:100; E1L3N, Cell Signaling Technology, Danvers, MA, USA), monoclonal mouse anti-PD-1 antibody (1:80 ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-Isl1/2 (Developmental Studies Hybridoma Bank, 1:100) and rabbit anti-β3-tubulin (Cell Signaling Technology, 5568S 1:200).
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bioRxiv - Genetics 2020Quote: ... and bound Myc bead (1/2 total amount) samples were analyzed by SDS-PAGE and immunoblotting with rabbit anti-Myc monoclonal antibody (1:2000; Cell Signaling) to detect Myc-tagged EXOSC5 and EXOSC2 ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were incubated with the polyclonal cleaved caspase 3 primary antibody (rabbit, 1:100; 9661, Cell Signaling, Frankfurt am Main, Germany) for 60 min at RT followed by ImmPRESS Reagent Kit ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the membranes were blocked with 5% bovine serum albumin in phosphate-buffered saline-0.1% Tween 20 and probed with rabbit anti-PPARγ antibody (#2443S, Cell Signaling Technology) and phospho-ser273 PPARγ (bs-4888R ...
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bioRxiv - Molecular Biology 2020Quote: ... Western blotting was performed according to standard procedures using the following antibodies and dilutions: H3K27me3 (1:1000 Cell Signaling C36B11, rabbit), H3K9me2 (1:1000 Upstate 07-441 ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were probed overnight at 4°C using primary antibodies (rabbit anti-p S6 (S235/236) 1:1000 (Cell Signaling #4858), rabbit anti-p-S6 (S240/244 ...
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bioRxiv - Neuroscience 2020Quote: ... including the primary antibodies (mouse anti-Iba1 antibody, Abcam, 1:400; rabbit anti-iNOS antibody, Abcam, 1:50; mouse anti-GFAP antibody, Cell signaling, 1:400 ...
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bioRxiv - Biochemistry 2020Quote: ... anti-rabbit antibody was purchased form Cell Signaling Technology (7074S) and anti-mouse antibody was purchased from Cell Signaling Technology (7076S).
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bioRxiv - Genetics 2020Quote: ... Primary antibody incubations were performed overnight at 4°C using the following antibodies: rabbit anti-GAPDH 14C10 (0.1 μg/mL, Cell Signaling 2118S), mouse anti-rhodopsin antibody clone 1D4 (1 μg/mL ...