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Citations for Stemcell Technologies :
4351 - 4400 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The blood was diluted with equal volume of Dulbecco’s phosphate-buffered saline (PBS) and overlaid onto Lymphoprep (Stem Cell Technologies). Plasma and white blood cells were collected after gradient centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Neural progenitor cells (NPCs) were cultured as a monolayer and differentiated using a Smadi Inhibition Kit from StemCell Technologies (#08581) requiring a minimum of 3 passages to become patterned NPCs ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2 μl/mL ROCKi and seeded into microwells (6-well AggreWellTM 400 plates, Stem Cell Technologies, cat. 34460) at a density of 800–1,000 cells per microwell using the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2023Quote: OVA-specific CD8+ T cells were isolated from the spleens and lymph nodes of OTI/Thy1.1 mice by negative selection (Stemcell Technologies) and labeled with CFSE for proliferation tracking ...
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bioRxiv - Neuroscience 2023Quote: ... Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies, Vancouver, BC, Canada) for 24 days ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies, Vancouver, BC, Canada) for 24 days ...
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bioRxiv - Immunology 2023Quote: ... CD8+ Tn or Teff cells isolated from LmOVA-infected mice by Thy1.1 positive selection kit (StemCell Technologies, Vancouver, BC, Canada) and were cultured immediately ex vivo in Seahorse XF medium (non-buffered DMEM 1640 containing 25mM glucose ...
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bioRxiv - Cancer Biology 2023Quote: ... The co-cultures were started by plating 2000 sorted LSKCD150+ to each well in Myelocult medium (M5300, Stem Cell Technologies) supplemented with 10-6 M hydrocortisone (74142 ...
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bioRxiv - Bioengineering 2023Quote: ... iPSC colonies were maintained in complete E8 media and passaged as necessary using ReLeSR passaging reagent (Stem Cell Technology, #05872). RNA was isolated from iPSC clones using the RNeasy Plus mini kit (Qiagen #74136 ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC, Canada). Isolated naïve CD8+ cells (1 x 106 cells/mL ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... counted and added at 1.2 million cells per microwell in AggreWell media containing 10 μM ROCK inhibitor (StemCell technologies 72302). Media was carefully changed the following day to remove ROCK inhibition ...
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bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2022Quote: ... CD34+ cells were then electroporated and transferred into erythroid expansion media containing SFEM and erythroid expansion supplement (Stemcell Technologies 02692) for 7 days and cultured at a density of 2e5-1e6 cells/ml ...
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bioRxiv - Genetics 2022Quote: ... CD34+ cells were then electroporated and transferred into erythroid expansion media containing SFEM and erythroid expansion supplement (Stemcell Technologies 02692) for 4 days ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA) and isolated using a negative isolation kit (EasySep™ Mouse B Cell Isolation Kit, #19854, StemCell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: CD4+ T naïve T cells from the spleens of WT mice were isolated by negative selection using the EasySep Mouse Naïve CD4+ T cell Isolation kit (StemCell Technologies). T cells were activated with 1 μg/ml plate-bound anti-CD3 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated by layering whole blood over Lymphoprep density gradient media (Stemcell Technologies; cat # 07851), resuspended in freezing media consisting of 90% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: Naïve CD4+ T cells from spleen and lymph nodes were enriched by negative selection using EasySepTM Mouse Naïve CD4+ T cell isolation kit (STEMCELL Technologies). 100,000 cells/well (50,000 CD45.1- cells and 50,000 CD45.1+ cells ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were enriched from spleen and lymph nodes of CD45.1+ mice by negative selection using EasySepTM Mouse CD4+ T cell isolation kit (STEMCELL Technologies), and FACS sorted as CD4+CD25- CD44loCD62Lhi cells ...
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bioRxiv - Immunology 2022Quote: ... and used directly for total CD4+ T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
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bioRxiv - Neuroscience 2023Quote: ... Neuronal induction was performed using a modification of a previously described protocol.44 Cells were dissociated with Accutase (STEMCELL Technologies) and plated in mTeSR supplemented with 10 mM ROCK inhibitor Y- 27632 and 2 mg/ml doxycycline on a Matrigel-coated 6-well plate ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... iPSCs were plated on Matrigel coated plates at ∼400k cells/cm2 in mTeSR plus medium (STEMCELL Technologies, Cat#100-0276). From day 0 to day 3 ...
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bioRxiv - Developmental Biology 2024Quote: ... a sign of neuroepithelial induction were placed onto a single dimple on embedding sheet (Stem Cell Technologies, cat. no. 08579) and 15 µl of Matrigel ...
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bioRxiv - Cancer Biology 2023Quote: ... The thawed cells were subsequently cultured in complete medium: IMDM supplemented with BIT 9500 Serum Substitute (Cat#09500, StemCell Technologies) and BIT (bovine serum albumin 4 g/L ...
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bioRxiv - Bioengineering 2023Quote: ... HepG2 spheroids were pre-formed by seeding 0.3 million cells per well in Aggrewell 800 (Stemcell Technologies, Vancouver, BC, Canada) and centrifuged at 1500 rpm for 10 min before getting transferred into an incubator at 37°C and 5% carbon dioxide ...
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bioRxiv - Neuroscience 2023Quote: ... Day 8 Ngn2 neurons were then seeded at a density of 100,000 cells per well in 100 µl droplets of CM2 Brainphys media [Brainphys (STEMCELL technologies), 1x Glutamax ...
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bioRxiv - Bioengineering 2024Quote: ... 5% CO2 for 48 hours prior to electroporation at a seeding concentration of 4.0 x 105 cells/mL in HSPC culture media (StemSpan SFEM II (StemCell Technologies) supplemented with penicillin/streptomycin (100 U/mL ...
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bioRxiv - Cell Biology 2023Quote: ... The reactions were incubated for 30 min at room temperature before mixing with 400,000 v1q cells in mTeSR (Cat. # 85850; STEMCELL Technologies) supplemented with 10μM Y-27632 (Cat ...
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bioRxiv - Physiology 2024Quote: ... coated with GelTrex (Fisher cat. A1413302; 1h at 37°C) and maintained in mTeSR media (Stem Cell Technologies cat. 85850). Rock inhibitor 10μM Y-27632 was added at seeding and after passages.
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bioRxiv - Cell Biology 2024Quote: ... ABSCs (P0-P2) were seeded at 8000-10,000 cells per well onto collagen-coated transwells in Pneumacult Ex Plus media (STEMCELL Technologies), supplemented with Primocin (Invivogen) ...
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bioRxiv - Cell Biology 2024Quote: F814 and WT bone marrow cells were cultured for 10 days in MesencultTM Expansion Kit for mouse (Stemcell tech, 05513) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and P14 cells were isolated on 7 dpi using the EasySep Mouse APC Positive Selection Kit II (#17667, STEMcell technologies) with a CD45.2 APC antibody (#17-0454-82 ...
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bioRxiv - Genetics 2024Quote: ... and plated at a density of 45,000-50,000 cells/cm2 in mTeSR™ plus medium with 10 µM Rock inhibitor Y-27632 (STEMCELL Technologies) on 6-well plates coated with Matrigel ...
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bioRxiv - Genomics 2024Quote: ... resuspending cell pellet in mTeSR Plus Basal Medium supplemented with 0.1% dihydrochloride ROCK Inhibitor (Stemcell technologies; Cat. No. Y-27632), counting and plating onto Geltrex-coated plates at the desired number.
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bioRxiv - Bioengineering 2024Quote: iPS11 hiPSCs (Alstem Cell Advancements, Richmond, CA, USA; iPS11) cultured with mTeSR-Plus (StemCell Technologies, Vancouver, BC, Canada; 100-0275) according to the manufacturer’s protocol on GelTrex-coated plates (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were seeded at a density of 0.5 cells/well in a 96-well plate coated with Matrigel and supplemented with cloneR (STEMCELL Technologies) during the first 96 hours after plating ...
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bioRxiv - Microbiology 2024Quote: ... and cultured overnight in mTeSR Plus medium supplemented with 10 µM Y-27362 ROCK inhibitor (Stem Cell Technologies, Cat.#72308). Differentiation was induced on day 0 by first washing cells with phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... 20ng/mL IL-3 and 20ng/mL GCSF) followed by 2 weeks in methylcellulose (MethoCult, Stem Cell Technologies, Cat. H4435). Stem cell frequencies were assessed by counting wells positive for cell growth.
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bioRxiv - Immunology 2024Quote: Naïve CD4+ or CD8+ T cells were isolated from mouse spleen and lymph nodes by using a mouse naïve CD4+ or CD8+ T cell isolation kit (STEMCELL Technologies). Purified naïve CD4+ or CD8+ T cells were labeled with CellTrace Violet (Invitrogen ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells were purified from mouse spleen and lymph nodes by using a mouse naïve CD4+ T cell isolation kit (STEMCELL Technologies) and polarized following the manufacturer’s protocol for Immunocult Mouse Th1 or Th2 differentiation supplements (STEMCELL Technologies) ...
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bioRxiv - Neuroscience 2024Quote: hiPSCs were differentiated into iMGLs as described previously with minor adaptations.52,73 hiPSCs were differentiated into hematopoietic precursors cells (HPCs) using the STEMdiff Hematopoietic kit (STEMCELL Technologies) largely as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... and cells positive for both constructs were sorted on a BD FACSAria III sorter into methylcellulose M3234 methylcellulose medium (StemCell Technologies). Cells were imaged using STEMvision (StemCell Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... at a density of 9000 cells per well in 100 μl of Embryoid Body Formation Medium (STEMdiff Cerebral organoid Kit, STEMCELL Technologies) with either addition of 10 μM Y-27632 (Tocris ...
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bioRxiv - Genomics 2020Quote: ... H1 cells were daily fed with fresh mTeSR1 media and passaged every 4-5 days using ReLeSR reagent (StemCell Technologies, 05872). Cells were dissociated into single cells with TrypLE Express (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: Peripheral blood samples from pediatric cancer and healthy patients were collected in EDTA tubes and peripheral blood mononuclear cells (PBMCs) were isolated from blood samples using SepMate tubes (Stemcell technology) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... and resuspended to a final concentration of 10 000 cells/50 μL in EB media: mTeSR™ Plus medium (StemCell Technologies), 50 ng/mL BMP-4 (R&D ...
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bioRxiv - Biochemistry 2022Quote: ... cardioids were maintained in CDM with insulin.21,31 Samples were collected 7 days post induction and dissociated using the STEMdiff cardiomyocyte dissociation kit (#05025, Stem Cell Technologies) and subsequently washed 3x with PBS.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: For sorting single cell suspensions were obtained as described prior and enriched with the EasySep mouse neutrophil isolation kit (STEMCELL Technologies). This semi-pure cell suspension was stained with fluorescently labelled antibodies via the methods described above and sorted with a 70uM nozzle (RNAseq and Giemsa stainings ...