Labshake search
Citations for Stemcell Technologies :
4151 - 4200 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: FA000010 (RUCDR/BiologyX) iPSCs were differentiated into hematopoietic precursors cells (HPCs) using the STEMdiff Hematopoietic kit (STEMCELL Technologies) largely by manufacturer’s instructions ...
-
bioRxiv - Biophysics 2023Quote: Neural stem cells were differentiated from hiPSCs using STEMdiff™ Neural Induction Medium (Cat. No. 05839, STEMCell Technologies) monolayer culture protocol as per the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2023Quote: ... hematopoietic lineage to obtain hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies, Catalog No. 05310). Next ...
-
bioRxiv - Neuroscience 2023Quote: ... rosette selection was performed by incubating for 2 hours with Neural Rosette Selection Reagent (Stem Cell Technologies, 05832), forcefully dislodging with warm DMEM/F12 ...
-
bioRxiv - Bioengineering 2024Quote: ... 500 and 1500 CD34+ cells were added to 1 mL of MethoCult™ H4034 Optimum medium (StemCell Technologies) and placed into one well of a 6-well plate ...
-
bioRxiv - Cell Biology 2024Quote: ... This was followed by cardiac specification using RPMI B27-medium containing 5 µM IWP-4 (Stem Cell Technologies) for another 3 days ...
-
bioRxiv - Developmental Biology 2024Quote: ... Organoids were manually transferred to a 0.4 µm pore culture plate (Corning, 3450; Stem Cell Technologies, 100-1026). Organoids received a pulse of TeSR-E6 supplemented with CHIR99021 for 1 hour ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD34+ cells enriched from CMML patients or healthy donors were cultured in StemSpan SFEM II medium (STEMCELL Technologies) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Approximately 1×10^5 cell per ml were plated in triplicate into M3234 incomplete methylcellulose medium (StemCell Technologies) supplemented with 0.1 ng/mL GM-CSF (Peprotech) ...
-
bioRxiv - Microbiology 2024Quote: ... CD34+ HPCs were differentiated using a commercial feeder-free hematopoietic differentiation kit (STEMdiff Hematopoietic Kit, Stem Cell Technologies). HEK293 and adult normal human dermal fibroblasts (NHDF ...
-
bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMCs) from consenting healthy blood donors were obtained using SepMate™ (STEMCELL technologies, Cat#85450). This research was approved by the McMaster Health Sciences Research Ethics Board ...
-
bioRxiv - Molecular Biology 2021Quote: ... or transparent centres were manually removed from the dish prior to dissociation with Gentle Cell Dissociation media (Stemcell Technologies). The IPSCs were then seeded and differentiated into cortical or dopaminergic neuronal progenitors or neurons.
-
bioRxiv - Bioengineering 2019Quote: ... Homogeneous and reproducible EBs were generated by using 24-well plate AggreWell™ 800 (Stem Cell Technologies, catalog 34815). In each well ...
-
bioRxiv - Cancer Biology 2019Quote: ... ALDH activity in the cells was measured by flow cytometry using the ALDEFLUOR kit (Stemcell technologies, Vancouver, BC, Canada). Cells with low and high levels of ALDH enzymatic activity (respectively ALDH- and ALDH+ cells ...
-
bioRxiv - Neuroscience 2019Quote: ... primitive neuroepithelia structures were formed and neural rosettes were selected with STEMDiff Neural Rosette Selection reagent (Stem Cell Technologies) and re-culture in suspension in NIM plus B27 supplement (Thermo Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... we evaluated tumor propagating cell numbers in hematopoietic malignancies using an ALDEFLUOR™ kit (StemCell Technologies, Vancouver, BC, CA) to detect ALDH1+ cells ...
-
bioRxiv - Cancer Biology 2019Quote: ... These cells were grown in suspension in NeuroCultTM NS-A Proliferation Kit with proliferation supplement (StemCell Technologies, Cat 05751), 20 ng/mL Recombinant Human Epidermal Growth Factor (EGF ...
-
bioRxiv - Neuroscience 2019Quote: ... with medium changes every 2-3 days and passages 1-2 times per week using ReLeSR (Stem Cell Technologies). Heteroplasmy levels were regularly measured to insure they were retained across passage numbers ...
-
bioRxiv - Cancer Biology 2019Quote: Neurosphere (sphere) cultures were established in serum-free media as described (9) (NeuroCult™ Media, Stem Cell Technologies, #05700) supplemented with human PDGF-AA (20 ng/mL ...
-
bioRxiv - Genetics 2019Quote: ... 5×104/mL bone marrow and 5×105/mL spleen cells were cultured with MegaCult-C Kit (STEMCELL TECHNOLOGIES) for 9 days ...
-
bioRxiv - Genomics 2019Quote: ... CD34+ HSPCs were incubated in StemSpan SFEM II medium with StemSpan Myeloid Expansion Supplement (Stem cell technologies, Vancouver, Canada) for 10 days ...
-
bioRxiv - Immunology 2021Quote: ... CD8- thymocytes were obtained by negative selection using the EasySep Mouse Streptavidin Rapid Spheres Isolation Kit (Stem Cell Technologies), then subjected to cell sorting ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary mouse microglia and M-MØP cells were exposed at set time points to anti-FcγRII/III (2.4G2) antibody at 5 µg/ml (Stemcell technologies), LPS (50 ng/ml ...
-
bioRxiv - Biochemistry 2020Quote: ... In short, TJP1-GFP(ZO-1-GFP) WTCs (Allen Institute, Seattle, WA) were plated in TeSR (Stem Cell Technologies) at 200,000 cells/well of Matrigel-coated 24-well plates on day (−1) ...
-
bioRxiv - Neuroscience 2020Quote: ... LIN28 and shRNA against p53 [28] in feeder- and serum-free conditions using TeSR-E7 medium (Stem Cell Technologies). Subsequently ...
-
bioRxiv - Cancer Biology 2020Quote: Both CD4+ and CD8+ cells were isolated from normal mouse splenocytes by immune-magnetic negative selection kit (Stemcell Technology, Vancouver ...
-
bioRxiv - Immunology 2021Quote: ... Both cell types were passaged every 7 days or as they reached 70-80% confluency using ReLeSR (STEMCELL Technologies). All iPSC and ESC lines used for these studies are listed in Table 1 along with the method of reprogramming and institution they were reprogrammed at ...
-
bioRxiv - Cancer Biology 2020Quote: GBM1 cells stably expressing FUCCI were washed with PBS and detached from the plate using Accutase (STEMCELL Technologies #07922) for ~10 min at 37ºC ...
-
bioRxiv - Cell Biology 2021Quote: Organoids and xenografts were processed to single cells and labelled using the Aldefluor Assay according to manufacturer’s (Stemcell Technologies) instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... PBMC cells were isolated from fresh whole blood by using SepMate-15 tubes with Lymphoprep gradient medium (StemCell Technologies) after centrifugation at 1200g for 20 minutes ...
-
bioRxiv - Developmental Biology 2022Quote: ... sorted PV CD34+CD36- cells were first expanded in StemSpan SFEM media with StemSpan CC100 (both from StemCell Technologies) for 3 days ...
-
bioRxiv - Physiology 2022Quote: ... Cells were then incubated on tissue culture plastic in PneumaCult-Ex Plus (Cat.# 05040, Stemcell Technologies, Vancouver BC Canada) containing 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco ...
-
bioRxiv - Bioengineering 2022Quote: ... Maintenance media was the same for all spheroids and consisted of BrainPhys Neuronal Medium (Stem Cell Technologies, cat #05790) supplemented with 1X N2 ...
-
bioRxiv - Neuroscience 2021Quote: ... the neuroepithelial sheet was detached from the plate using STEMdiff Neural Rosette Selection Reagent (Stem Cell Technologies, Cat.No. 05832). From the following day until DIV 27 cultures were grown in Neuronal Maintenance Media (N2B27 supplemented with 20ng/μl hFGF ...
-
bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
-
bioRxiv - Immunology 2021Quote: ... duplicate samples containing 500 sorted cells were plated in 1.5 ml MethoCultTM GF M3434 (STEMCELL Technologies, Catalogue no.- 03434) which already contains SCF ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were cultured in mTeSR plus medium supplemented with 10 µM Rho-associated protein kinase (ROCK) inhibitor (Stemcell Technologies). After overnight incubation ...
-
bioRxiv - Developmental Biology 2022Quote: ... Supernatants were removed and cells were resuspended in Noci-2 medium + CEPT and plated at 5.5 x 106 cells/well into 6-well AggreWell 800 plates according to manufacturer’s instruction (STEMCELL Technologies) for free-floating suspension culture and nocisphere formation ...
-
bioRxiv - Immunology 2022Quote: ... Naïve splenic B lymphocytes were purified by negative selection using the EasySep Mouse B Cell Isolation Kit (STEMCELL Technologies). Primary B cells from each transgenic mouse line were cultured in RPMI 1640 with L-glutamine supplemented with 10% fetal bovine serum ...
-
The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... Dzierzak (Erasmus University MC, Rotterdam, The Netherlands) and maintained in 50% Myelocult M5300 medium (Stem Cell Technologies, Grenoble, France), 35% α-MEM ...
-
bioRxiv - Immunology 2020Quote: CD4 or CD8 T cells were isolated from spleen using magnetic beads as described by manufacturer’s protocol (STEMCELL technologies). 4000 cells in ratio 1:1 were transferred into WT mice 1 day prior to LCMV-Cl13 infection.
-
bioRxiv - Developmental Biology 2019Quote: ... Neurospheres were grown on non-tissue culture treated plates with mouse NeuroCult proliferation media (Stem Cell Technologies; Seattle, WA) containing 10 ng/ml bFGF ...
-
bioRxiv - Cell Biology 2020Quote: ... hiPSC colonies were collected from a 6 well MEF-coated plate using Gentle Cell Dissociation Reagent (Stemcell Technologies, CA) and seeded into Matrigel (BD ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were seeded and cultured in EB medium consisting of complete mTeSR1 supplemented with 10 µM Y27632 (StemCell Technologies), 50 ng/mL BMP-4 ...
-
bioRxiv - Immunology 2020Quote: ... FcγRII or FcγRIII block was performed by addition of receptor specific mAbs (Stem cell technologies, IV.3 and 3G8) at a 1:100 dilution at the beginning of the incubation period ...
-
bioRxiv - Neuroscience 2020Quote: ... Media was changed daily and cells were split at a 1:6 ratio using Collagenase IV (Stemcell Technologies, #07909) every 5-6 days.
-
bioRxiv - Cell Biology 2021Quote: NALM6 wildtype and SF3B1-H662Q cells were purchased from Horizon Discovery and grown in RPMI-1640 medium (STEMCELL Technologies) supplemented with 10% fetal bovine serum (Life Technologies ...
-
bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
-
bioRxiv - Immunology 2020Quote: ... and used directly for total T cell enrichment by negative magnetic selection using Easysep magnetic separation kits (STEMCELL Technologies). Cell suspension was stained with anti-CD4 (RPA-T4) ...
-
bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).