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Citations for Stemcell Technologies :
4251 - 4300 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Differentiation of iPSCs into iPSC-derived hematopoietic progenitor cells (iHPCs) was carried out using STEMdiffTM Hematopoietic kit (STEMCELL Technologies) as previously described (4 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were thawed and grown in a T75 flask using PneumaCult-Ex Plus medium (Stemcell Technologies, Cologne, Germany). Once confluency reached 60–80% ...
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bioRxiv - Cancer Biology 2023Quote: ... HSPCs (Lin- cKit+ Sca1+) were FACS-sorted and cultured in liquid culture using StemSpan (Stem Cell Technologies, Vancouver, Canada) supplemented with Il-3 (10ng/mL ...
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bioRxiv - Microbiology 2023Quote: ... and resuspended in the required volume of Formation Medium prepared using the STEMdiff Cerebral Organoid Kit (Stem Cell Technologies) supplemented with 50 μM Rho-associated protein kinase (ROCK ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T lymphocytes were negatively selected for using the EasySep™ Mouse T Cell Isolation Kit (StemCell Technologies #19851) and cultured in RPMI containing L-glutamine (Corning # MT10040CM ...
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bioRxiv - Cell Biology 2023Quote: ... After 5 weeks the H5100 medium was replaced with Methocult H4434 Classic methylcellulose medium (100µl/ well, STEM CELL technologies). Wells that contained at least one colony after 10-14 days were considered positive.
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bioRxiv - Developmental Biology 2024Quote: ... and 2 µM A83-01, an inhibitor of TGFβ type I receptors (ALK4, ALK5, ALK7) (Stem Cell Technologies, 72022) for 5 days in vitro to induce embryoid body (EB ...
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bioRxiv - Developmental Biology 2024Quote: ... and were plated in a 6-well plate containing 10 µM Rock inhibitor Y-27632 (Stem Cell Technologies, 72302). Twenty-four hours post transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and Naïve CD4+ T cell were isolated from splenocytes with commercial kits (STEMCELL Technologies, Cat No. 19852, 19853, 19765) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD8 T cells were purified to >90% from the spleens of donor strains using negative selection (StemCell Technologies) and stained with CTV or CTFR proliferation dyes (Molecular Probes ...
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bioRxiv - Cell Biology 2023Quote: Mature organoids are collected by rinsing the maintenance well using 1-2 ml cold DMEM/F12 (Stem Cell Technologies) + 15 mM HEPES + 1x GlutaMax (1:100 35050-038 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD45.1/CD45.2+ P14+ CD8+ T cells were purified from spleens using the StemCell CD8+ isolation kit (STEMCELL Technologies) and 500 were adoptively transferred into CD45.1+ mice ...
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bioRxiv - Cancer Biology 2023Quote: ... the medium refreshment of the 293FT cells was done using the StemSpan SFEM II medium (Stemcell Technologies, cat# 9655). 3-4 days after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... We prepared triplicate CFU assays by seeding 50,000 cells/1.5 mL of semi-solid Methocult H4435 Enriched media (StemCell Technologies) in 35 mm culture dishes (Greiner ...
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bioRxiv - Genetics 2023Quote: ... The pellet was resuspended at a concentration of 1.2×106 cells/mL in neuron media [Brainphys (StemCell Technologies #05790), 1xN2 (ThermoFisher #17502-048) ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells from Thy1.1+ P14 mice (PBMCs) were enriched using a CD8 MACS-negative selection kit (STEMCELL Technologies). ∼35,000 P14 CD8+ T cells were transferred intravenously into naïve Thy1.2+ C57BL/6 recipient mice ...
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bioRxiv - Cancer Biology 2024Quote: ... Bone spicules were digested for 60 minutes in PBS supplemented with 2.5mg/mL Collagenase Type I (Stem Cell Technologies) and 20% FBS ...
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bioRxiv - Genomics 2024Quote: Female H9 (WA09; RRID: CVCL_9773) hESCs were obtained from WiCell and cultured in either mTeSR1 (Stem Cell Technologies 85850) for at least one passage before differentiation into CNCCs or mTeSR Plus (Stem Cell Technologies 100–0276 ...
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bioRxiv - Microbiology 2024Quote: Both wild type (WT) and RFP labeled iPSCs were differentiated to HPCs using STEMdiff hematopoietic kit (Stem Cell Technologies). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Colon organoids were harvested for immunoblotting by dissolving the Matrigel with 500 µl Gentle Cell Dissociation Reagent (Stemcell Technologies, UK) for 1 minute ...
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bioRxiv - Genetics 2021Quote: ... A karyotypically normal human male iPSC line from an individual without known disease (33362D, passage <30) was seeded at 100,000 cells per well and maintained on mTESR1 media (Stemcell Technologies). On day three post-plating and upon reaching 50% confluence ...
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bioRxiv - Genetics 2021Quote: ... day-30 enriched iPS-CM cultures were harvested using 0.25% Trypsin and stored in CryoStor Cell Freezing Medium (STEMCELL Technologies) in liquid nitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... The cell pellet was resuspended into HF buffer (HBSS, 2% fetal bovine serum, 10mM Hepes pH 7.2) and NH4Cl (StemCell Technologies). The cell pellet was recovered after 5 min of centrifugation at 1000rpm and resuspended in organoid medium for organoid culture ...
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bioRxiv - Developmental Biology 2021Quote: ... c-Kit+) from neonatal wild-type mice were FACS sorted and cultured in MethoCultTm medium (GF M3434, Stem cell technologies) in 35mm dishes ...
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bioRxiv - Developmental Biology 2020Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent and cryopreserved in mFreSR according to manufacturer’s instructions (STEMCELL Technologies). All lines were expanded and stored at low passage number.
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Molecular Biology 2020Quote: ... UCB mononuclear cells were treated with Aldefluor reagent according to the manufacturer’s manual (ALDEFLUOR™ Kit, Stemcell Technologies, Cat.No. 01700). The expressions of CD34 ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... A small number of cells (3,000 to 4,000 cells) were set aside for use in the colony forming unit assay using M3434 media (STEMCELL Technologies) and analyzed 7-10 days later.
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bioRxiv - Developmental Biology 2022Quote: Day 15 H9 hESC-derived enteric crestospheres were dissociated into single cells (accutase, Stemcell Technologies, 07920, 30 min, 37 °C), resuspended in ENC medium and were transferred into 384-well plates ...
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bioRxiv - Immunology 2019Quote: ... LV-CAR T cells were expanded by stimulation with an antibody cocktail (anti-CD3/CD28; STEMCELL Technologies; cat. no. 10971) combined with IL-2 (50 U/ml ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was subsequently plated in a 96-well V-bottom at a density of 20,000 cells with Neural Induction Medium (StemCell Technologies) supplemented with dual SMAD inhibitors SB321542 (10 μM ...
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bioRxiv - Neuroscience 2019Quote: The protocol for iMGL derivation was adapted from 55 with modifications from the StemDiff Hematopoietic Kit (Stem Cell Technologies, #05310). H9 CX3CR1-TdTomato cells were cultured on vitronectin (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... Live GFP-high cells were sorted on Aria II at low flow rates and reaggregated in Aggrewell-400 (StemCell Technologies) at 1,000 cells per cluster in CMRL containing 10% FBS ...
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bioRxiv - Cancer Biology 2019Quote: ... Prior to use for transplanting mice the cells were thawed in Iscove’s modified Dul-becco medium with 10% FBS (both STEMCELL Technologies) and 10 mg/mL DNase I (Sigma Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... CD4 and CD8 T cells were isolated from a mouse using CD4 and CD8 negative selection kits respectively (StemCell Technologies) and were labelled with the caged strand hybridized to the Ab-oligo conjugate ...
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bioRxiv - Cancer Biology 2019Quote: ... Tumors were mechanically dissociated and digested for 1 h with 1x collagenase/hyaluronidase (10X Solution, Stem Cell Technology, Cambridge, MA) containing DNase I (2 U/ml ...
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bioRxiv - Microbiology 2021Quote: ... undifferentiated iPSCs were cultured until confluent and then passaged on Day 0 using gentle cell dissociation reagent (GCDR, StemCell Technologies) to achieve single cell suspensions and replated at 1×106 cells per well of a Matrigel coated 6-well plate ...
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bioRxiv - Bioengineering 2021Quote: ... The microcapsules carrying HUES-8 cells were collected into 15 mL tube filled with 5 mL E8 medium (STEMCELL Technologies), then distributed into a 6 well-plate at density of 2000 capsules/well and incubated at 37 □ with 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... A hypoxic environment was created by placing the cell culture vessels in a hypoxia incubator chamber (STEMCELL Technologies, Cambridge MA), which was then filled with a gas mixture containing 2% O2 ...
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bioRxiv - Bioengineering 2020Quote: ... Initial enrichment for HSPC was performed with the EasySep Mouse Hematopoietic Progenitor Cell Enrichment Kit and Magnet (Stemcell Technologies, CA). Further isolation was performed to obtain a population of the Lin− Sca-1+ c-kit+ (LSK ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on 5% laminin at 50,000 cells per well of 6 well-plate and incubated in full growth medium: PneumaCult-Ex medium (Stemcell Tech), glutamax supplement (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were counted and 2 million were cultured for 6 days in hematopoietic expansion media -Stemspan H3000(Stemcell Technologies), with the addition of EPO (R&D) ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 2mL per well T0 media and deposited into AggreWell 400 6-Well plates (StemCell Technologies 34425) prepared with Aggrewell Rinsing Solution (StemCell Technologies 07010 ...
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bioRxiv - Bioengineering 2021Quote: ... the cuvettes were allowed to rest for 15 minutes before the T cells were gently moved to antibiotic-free medium containing 1µg/mL DNase (StemCell Tech) at 37°C ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated with a cocktail of lineage specific antibodies (CD5, CD11b, CD19, B220, Gr-1 and TER119, Stemcell Technologies) for negative selection of Lin-cells using an EasyStep immunomagnetic column (Stemcell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: The premalignant mammary glands from 4.5-month-old Neu and Neu/PTx mice were dissociated using the Mammary epithelial cell enrichment kit (StemCell Technologies). The dissociated single cells were first stained with biotin-labeled anti-mouse CD31 ...
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bioRxiv - Bioengineering 2022Quote: ... Antibody-bound cells were depleted with streptavidin RapidSphere™ magnetic beads according to the manufacturer’s instructions (STEMCELL Technologies; Vancouver, Canada). Cells with >90% purity were used for experiments ...
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bioRxiv - Developmental Biology 2022Quote: ... Transfected cells were placed on a Matrigel-coated culture dish and incubated in mTeSR1 medium (STEMCELL Technologies Inc., Vancouver, Canada) containing 10 μM Y27632 (ROCK inhibitor ...
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bioRxiv - Cell Biology 2022Quote: ... M2 polarization was achieved by stimulating MΦ cells with 20 ng/mL of interleukin 4 (IL4; Stemcell Technologies, Cat#78045) and interleukin 13 (IL13 ...