Labshake search
Citations for Stemcell Technologies :
4551 - 4600 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Double-positive cells were plated into a matrigel-coated 6 cm petri dish in mTeSR supplemented with 1x CloneR (Stemcell Technologies). Individual colonies were manually picked into 96 wells ∼10 days after transfection ...
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bioRxiv - Microbiology 2023Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, France) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, Canada). Culture supernatants were screened using ELISA (see below ...
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bioRxiv - Immunology 2023Quote: ... ETRAMP-specific CD4+ T cells were enriched using APC- and PE-conjugated tetramers and EasySep APC and PE Positive Selection Kits (STEMCELL Technologies). Cells were then stained with fluorescent dye-conjugated antibodies (see Table I ...
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bioRxiv - Cell Biology 2023Quote: ... For time first division Single cell LIN-CD34+ CD38- CD9+ or CD9- cells sorted in to the round bottomed 96 well plates and culture in SFEM (StemCell Technologies), supplemented with SCF (10ng/ml) ...
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bioRxiv - Cell Biology 2023Quote: For cell growth assay 100 LIN-CD34+CD38- CD9+ or CD9- cells were sorted and seeded in 96 well plate and culture in StemSpan Serum –Free Expansion Medium (SFEM, StemCell Technologies) supplemented with 100u/ml penicillin (Hyclone ...
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bioRxiv - Microbiology 2023Quote: ... Primary airway cells derived from single patient sources were first expanded on plastic in Pneumacult-Ex Plus medium (Cat No. 05040, StemCell Technologies), then seeded (3.3 × 104 cells / well ...
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bioRxiv - Cancer Biology 2023Quote: CD8 T cells were isolated from the lymph node of naïve mice with unactivated Confetti cassette using EasySep™ Mouse CD8+ T Cell Isolation Kit (StemCell Technologies) and macrophages were sorted from the tumors via FACS sorting ...
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bioRxiv - Immunology 2023Quote: Small resting B cells were isolated from spleen with a discontinuous Percoll gradient and subsequent purification using EasySep Mouse B cell Isolation Kit (StemCell Technologies). B cells were stimulated with 3mM CpG (Sigma) ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from peripheral lymph nodes (inguinal, axillary, brachial and superficial cervical) by negative selection using the EasySep Mouse CD4+ T cell Isolation Kit (StemCell Technologies). CD4+ T cells were stimulated in 96 well flat-bottomed plates coated with 20mg/ml αCD3 (clone 145-2C11 ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... OT-I+/- or total CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection using the EasySepTM Mouse CD8+ T cell isolation kit (#19753, StemCell Technologies). For in vitro experiments ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then dissociated and resuspended in cloning media consisting of mTeSR-Plus supplemented with 1X CloneR2 (StemCell Technologies; 100-0691) and mixed gently with a P1000 pipette followed by passing through a 40 μm cell strainer in order to generate a single-cell suspension ...
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bioRxiv - Cancer Biology 2024Quote: Bone marrow mononuclear cells were obtained from healthy donors with informed consent and compliance with relevant ethical regulations (AllCells and STEMCELL Technologies). All healthy cells used in this study were cryopreserved ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were kept in MEF medium for 48 h and then the medium was changed to mouse embryonic stem cell (mESC) medium: DMEM with the addition of 15% mouse ESC maintenance FBS (STEMCELL Technologies), 100 units ml−1 ESGRO mouse leukaemia inhibitory factor (mLIF ...
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bioRxiv - Cancer Biology 2024Quote: Primary mouse NK cells were isolated from spleens of 8-12-week-old wild-type or Prf1-/- mice using the negative selection EasySep mouse NK cell isolation kit (STEMCELL Technologies) or positive selection CD49b (DX5 ...
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bioRxiv - Microbiology 2024Quote: ... BCi-NS1.1 and NHBE cells were first expanded on plastic in Pneumacult-Ex or Pneumacult-Ex Plus medium (no. 05008 or 05040, StemCell Technologies). Airway cells were then seeded (3.3 × 104 cells/well ...
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bioRxiv - Bioengineering 2024Quote: ... and reseeded as a single cell suspension at a density of 1.5×104 cells/cm in mTeSR™ Plus supplemented with 10 μM Y-27632 RHO/ROCK pathway inhibitor (72304, STEMCELL Technologies). Media was replaced with Y-27632 free mTeSR™ Plus after 24 hours.
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bioRxiv - Cancer Biology 2024Quote: The ALDH activity of oral cancer cells co-cultured with CAF was measured using the ALDEFLUOR assay kit (Cat. #01700, StemCell Technology), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... ECs and FBs were harvested from cell culture flasks after five days of culturing post-thawing using ACCUTASE (07922; STEMCELL Technologies) and resuspended in VascuLife supplemented with thrombin (T4648 ...
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bioRxiv - Microbiology 2024Quote: WA09 embryonic stem cells (WiCell, Madison, WI, USA) were expanded and maintained at 37°C/5% CO2 in mTeSR-E8 culture medium (STEMCELL Technologies) on Nunclon cultureware coated with LDEV-free Geltrex (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions harvested from cell culture or isolated from spleen or mediastinal lymph nodes were incubated with 10% Rat serum (STEMCELL Technologies), Fixable Viability Dye (ebioscience ...
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bioRxiv - Cancer Biology 2021Quote: Ten thousand cells of RPMI-2650 and Detroit celllines were seeded in 24-well Ul-tra-Low Attachment Plates (Costar) containing 1 mL of MammoCult basal medium (Stem Cell Technologies) supplied with 10% MammoCult proliferation supplement ...
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bioRxiv - Cancer Biology 2021Quote: Cells were plated on uncoated low-adhesion 96-well plates in a two-fold dilution series spanning from 2000 down to 4 cells per well in NeuroCult NS-A media (StemCell Technologies, #05751) containing doxycycline at 2 μg/mL ...
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bioRxiv - Immunology 2021Quote: OVA (SIINFEKL)-specific CD8+ T cells were isolated from the spleen and lymph nodes of healthy 6-8-week old OT-I mice using EasySep™ Mouse CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Murine T-cells were isolated from spleens of healthy 6-8-week old C57BL/6 mice using EasySep™ Mouse CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2020Quote: ... Two conventional and established conditions were used as indicated throughout the figures and manuscript: TeSR conditions on Matrigel coated plates (Stem Cell Technologies) or FGF/KSR conditions on MEF substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... were a gift from the Nik-Zainal lab (University of Cambridge, UK) and cultured on non-tissue culture treated plates (Stem Cell Technologies) pre-coated with 10 μg/mL Vitronectin XF (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2021Quote: The mCar-GFP and Crx-mCherry iPSC lines (kind gift from Olivier Goureau – see (23)) were maintained in mTeSR1 (Stem cell technologies) on matrigel coated plates and split using ReleSR at room temperature (Stem cell technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: Cord blood CD34+ hematopoietic stem cells were purchased and cultured in StemSpan II media with the MK supplemental cytokines (StemSpan II, Stemcell Technologies, Inc.), according to the culture and differentiation protocols from Stemcell Technologies (Stemcell Technologies Inc ...
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bioRxiv - Physiology 2020Quote: ... coated tissue culture dishes as per manufacturer recommendation with either mTeSR medium with supplementation or mTeSR PLUS medium with supplementation (Stem Cell Technologies). Contractile cardiomyocytes were differentiated using a high-density monolayer format as previously described (43) ...
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bioRxiv - Immunology 2019Quote: ... CD8 T cells were purified from the spleens of early Nrp1 deleted (d28) or late Nrp1 deleted (d34) Nrp1 E8i-CreERT2 R26-mice using EasySep™ Mouse CD8 T Cell Isolation Kits (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: Skin fibroblasts of MELAS subject #1 with the pathogenic variant m.3243A>G in MT-TL1 (tRNALeu(UUR were reprogrammed by the in house Radboudumc Stem Cell Technology Centre ...
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bioRxiv - Neuroscience 2019Quote: ... medium was refreshed weekly by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies) and 1% pen/strep (BP-complete medium).
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bioRxiv - Genetics 2019Quote: 1×10^5 cells from the RF of xenotransplanted mice were transferred to 1ml of MethoCult H4034 Optimum methylcellulose medium (StemCell Technologies, 04034) and plated onto a 35mm dish for human specific colony formation ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 10% fetal bovine serum and subsequently resuspended at a concentration of 5×105 cells/mL in 4-5mL of MethoCult H4230 methylcellulose (STEMCELL Technologies, 04230) containing penicillin/streptomycin ...
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bioRxiv - Bioengineering 2019Quote: ... 20.000 ASCs on their 6th passage were seeded per well on day 1 and cultured for 24 hours before exposure to Mesencult MSC medium (Stem Cell Technologies) or neurogenic differentiation medium ...
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bioRxiv - Bioengineering 2020Quote: ... to sort single cells into Matrigel-coated 96-well plates with each well containing 150 μl of mTeSR1 supplemented with CloneR (STEMCELL Technologies, #05888). Following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... after which cells were replated in E8 containing p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (2 μM; Stemcell Tech, 72307).
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... we first patterned the hiPSCs with a mesenchymal protocol as previously described.(Lam, et al., 2014) Cells were treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Bioengineering 2021Quote: ... we first directed the hiPSCs towards an intermediate mesenchymal fate using a previously described protocol,[84] wherein cells are treated with 5 µM CHIR99021 (STEMCELL Technologies, 72052) in Roswell Park Memorial Institute Medium 1640 (RPMI 1640 ...
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bioRxiv - Neuroscience 2021Quote: ... the sample was sorted using an APC-conjugated antibody to NOTCH1 (eBiosciences #17-5765-80) followed by APC magnetic selection using the EasySep kit (Stem Cell Tech) as per instructions and plated in PneumaCult medium with a SMAD inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... These cells were cultured in mTeSR™1 medium (mTeSR™1 Basal Medium with mTeSR™1 5× Supplement (Stemcell Technologies) and 1% penicillin-streptomycin (P/S ...
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bioRxiv - Neuroscience 2020Quote: ... the glucose concentration of LN was adjusted to 10 mM and the following supplements were added freshly before use: 20 ng/ml BDNF (Stem Cell Technologies), 20 ng/ml GDNF (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... organoids were either cultured in HN+A or were transferred gradually to Low-Nutrient medium (LN) consisting of BrainPhys Neuronal Medium (Stem Cell Technologies), 2% B27+A (50X ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were counted using a hemocytometer and seeded in 35 mm dishes at 500 cells/dish in MethoCult H4435 Enriched (StemCell Technologies 04435) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) over the course of 11 days using the STEMdiff Hematopoietic kit (STEMCELL technologies, 05310). Non-adherent cells were then collected and analyzed ...
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bioRxiv - Microbiology 2021Quote: Cortical organoid cultures were differentiated from the healthy iPSC cell line according to the specification of the cerebral organoid kit from StemCell Technologies (#08570) that relies on an established protocol (54) ...
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bioRxiv - Neuroscience 2020Quote: The human embryonic stem cell (hESC) line harbouring the GPF reporter at the 3’UTR of CRX locus was expanded in mTeSR™1 (StemCell Technologies) at 37°C and 5% CO2 on 6 well plates pre-coated with Low Growth Factor Matrigel (Corning) ...