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Citations for Stemcell Technologies :
4601 - 4650 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 100ug were added to 50K GMPs sorted the day before and cultured in StemSpan SFEMII supplemented with 1% L-Glutamine and Penicillin/Streptomycin with no cytokines (Stem cell technologies). Cells were cultured in a humidified incubator at 37°C and 5% CO2 for 12 hours and then washed twice with PBS-2%FBS with 7AAD ...
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bioRxiv - Cell Biology 2020Quote: ... Neural rosettes were derived between day 3 to day 5 by addition of STEMdiffTM Neural Rosette Selection Reagent (05832, Stem Cell Technologies) for 45 mins-1 hr at 37oC ...
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bioRxiv - Cell Biology 2020Quote: ... was used for careful collection and deposition of the EBs on the top of a 37 μm reversible strainer (Stem Cell Technologies). Multiple cycles were performed with E6 until all the EBs were removed ...
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bioRxiv - Cell Biology 2021Quote: ... CRU frequency was calculated by applying Poisson statistics to the rate of negative recipients at different dilutions using L-Calc software (Stem Cell Technologies).
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bioRxiv - Immunology 2022Quote: ... FBM mononuclear cells were isolated by centrifugation for 30 minutes at room temperature on a Ficoll gradient (StemCell Technologies, Vancouver, BC, Canada) and red blood cells were removed using the RBC lysis buffer (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 07180)-coated tissue culture plates and split in a ratio of 1:6 to 1:12 every four to six days with Gentle Cell Dissociation Reagent (STEMCELL Technologies, 07174). Alveolar differentiation was induced as previously described (Riva et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... All tissues were subject to removal of red blood cells (RBCs) using immunomagnetic negative selection with the EasySep RBC Depletion kit (STEMCELL Technologies, 18170). 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... Stem cells were maintained as previously described (Spence et al., 2011) and grown in mTeSR Plus media (StemCell Technologies, Cat#100-0276).
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bioRxiv - Immunology 2022Quote: ... CAR T cells were positively selected for truncated CD19 using the EasySep™ CD19 Positive Enrichment Kit I or II (StemCell Technologies) (for PSCA-CAR T cells ...
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bioRxiv - Cancer Biology 2022Quote: The single cell suspension of dissociated tumors were freshly sorted via immunomagnetic sorting and 1x103 EpCAM+/ABCG2+ or EpCAM+/ABCG2-CSCs were seeded in methylcellulose medium (Methocult M3134, Stem Cell Technologies) as described previously [1] ...
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bioRxiv - Developmental Biology 2021Quote: hESCs were single-cell seeded onto 6-well Matrigel-coated plates at 2*106 cells/well for ectoderm and endoderm conditions and 5*105 cells/well for mesoderm condition and treated with the appropriate differentiation medium using the STEMdiff Trilineage Differentiation Kit (StemCell Technologies #05230) according to manufacturer instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed in Methacarn (1/3/6 mixture of acetic acid/chloroform/methanol) overnight at room temperature and stained with carmine alum (Stem Cell Technologies), or fixed in 4% paraformaldehyde overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... 1 or 2 EBs were transferred to a well of a 24-well ultra-low attachment plate containing Induction Medium (Stem Cell Technologies). The EBs were kept in the induction Medium for 2 days and next they were transferred into Matrigel (Corning ...
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bioRxiv - Cancer Biology 2021Quote: To determine the re-plating ability of HSPCs Inpp4b+/+ and Inpp4b-/- we plated bone marrow from age- and sex-matched mice in Methocult GF M3434 (Stem Cell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... NK cells were allowed to recover at a density of 1.5 × 106 cells/mL in antibiotic-free medium containing 1 ug/mL DNase I solution (STEMCELL Technologies, Cambridge, MA), and were then cultured in complete NK cell medium at a density of 6 × 105 cells/mL ...
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bioRxiv - Developmental Biology 2022Quote: Each interstitial cell fraction in in vitro culture on day 3 or day 6 was digested with Accumax™ (STEMCELL Technologies #07921) for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... five to seven day old iPSCs cultures were then used to generate cluster of differentiating 43 positive (CD43+) hematopoietic progenitor cells (HPCs) following a 12 day commercially available kit (STEMdiff Hematopoietic Kit; Stemcell Technologies # 05310). IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923 ...
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bioRxiv - Genetics 2020Quote: ... Neural induction of hES H1 cells expressing different shRNA constructs was performed using STEMdiff™ Neural Induction Medium (#05831, StemCell Technologies Inc.) in combination with a monolayer culture method according to the manufacturer’s technical bulletin (#28044 ...
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bioRxiv - Cancer Biology 2019Quote: Single cells were FACS-isolated into flat bottomed 96-well plates containing 100 μl of MethoCultTM H4435 Enriched (StemCell Technologies Cat#04435). Colonies were visually inspected and classified 11-14 days after plating ...
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bioRxiv - Immunology 2020Quote: PBMCs of anonymous healthy donors were isolated from buffy coats purchased from the Beijing Red Cross Blood Center using density gradient cell separation by Ficoll (Lymphoprep™, STEMCELL Technologies) following the protocol approved by the Institutional Review Board of School of Medicine ...
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bioRxiv - Molecular Biology 2019Quote: ... HSPCs were isolated from Tie2-Cre control (wild-type) or Tie2FF1 (JAK2V617F) and cultured overnight in StemSpan serum-free expansion medium (SFEM) (Stem Cell Technologies) containing 100 ng/mL recombinant mouse SCF ...
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bioRxiv - Cancer Biology 2019Quote: Bulk tumors were mechanically minced into a slurry with sterile scalpels and then enzymatically digested in Media 199 containing 300 U/ml Collagenase and 100 U/ml Hyaluronidase mixture (Stem Cell Technologies) and 125 U/ml DNAse-I (Cedarlane ...
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bioRxiv - Cancer Biology 2020Quote: ... Fourth and fifth mammary gland pairs were resected from mice and were enzymatically digested with collagenase/hyaluronidase (Stem Cell Technologies, cat #07919) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was refreshed every 2-3 days and hiPSCs were passaged twice per week using an enzyme-free reagent (ReLeSR, Stem Cell Technologies).
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bioRxiv - Immunology 2021Quote: ... induced tdTomato expressing thymocytes were labelled with biotinylated anti-CD4 and -CD8 antibodies and were enriched by immunoseparation of bulk thymocytes with Easysep Streptavidin Rapidspheres (Stem Cell Technologies) to reduce CD4+ and CD8+ cells ...
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bioRxiv - Cancer Biology 2019Quote: HUDEP-2 cells were acquired from the Nakamura Lab at the RIKEN BioResource Center in Japan and were cultured in StemSpan SFEM (Stem Cell Technologies) supplemented with 50ng/ml SCF (R&D Systems) ...
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bioRxiv - Immunology 2020Quote: ... C57BL/6 mice by use of magnetic separation with the EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit II (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: T lymphocytes were isolated from the spleen of C57BL/6J mice using a negative selection isolation kit (EasySep™ Mouse T Cell Isolation Kit, StemCell Technologies). Cells were plated in a 48 wells plate (Corning ...
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bioRxiv - Genomics 2020Quote: ... a piece of frozen tumor tissue was placed into a well of a 6-well plate (Stem cell Technologies, catalog no. 38015) with 1 mL of either CST ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged in clumps of 8-10 cells and seeded in mTeSR supplemented with the Rho-associated protein kinase inhibitor γ-27632 (STEMCELL Technologies) at 10 µM for the first day to improve survival ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were isolated from SPLNs of 5 donors from each group using a mouse CD4+ T cell isolation kit following manufacturer’s instructions (STEMCELL Technologies, Vancouver, BC). CD4+ T cells from NT and CPZ donors were injected intraperitoneally into RAG1 KO recipient mice ...
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bioRxiv - Immunology 2021Quote: ... CD8+ and CD4+ cells were enriched from the non-adherent fraction by untouched negative selection (Stemcell Technologies, Cat 17952 and Cat 17953). To generate moDC ...
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bioRxiv - Immunology 2021Quote: ... CD4+ CD62L+ naïve T cells were isolated from 8-12 week-old mice using CD4 negative enrichment kits (Stemcell Technologies, Vancouver, Canada) and CD62L microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: Whole blood was collected in heparin containing vacuutubes and 12μL of 0.5M EDTA with 1mL of PBS+2% FBS and 50μL of RBC of EasySep RBC Depletion spheres (EasySep ™ RBC Depletion Reagent: 18170, Stem Cell Technologies) were added ...
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12-Lipoxygenase Governs the Innate Immune Pathogenesis of Islet Inflammation and Autoimmune DiabetesbioRxiv - Immunology 2021Quote: ... Naïve T cells were isolated and purified from spleen and lymph nodes of C57BL/6J mice using EasySep™ Mouse CD4+ T Cell Isolation Kit (Stemcell Technologies).
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bioRxiv - Genetics 2020Quote: ... emerging iPSC colonies that were large enough to isolate were manually picked using a sterile pulled glass pipette into VTN-coated 6-well tissue culture plates containing complete TeSR-E8 media (TeSR-E8 basal medium with 25X TeSR-E8 supplement) (Stem Cell Technologies) using a SMZ1000 Nikon dissecting microscope (Nikon ...
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bioRxiv - Genetics 2020Quote: ... The PBMCs were either resuspended immediately in complete Erythroid Expansion Medium (StemSpan-SFEM II with 100X StemSpan Erythroid Expansion Supplement) (Stem Cell Technologies) for reprogramming or resuspended at a density of 0.5 x 106 PBMCs per cryo vial in PSC cryomedium (Gibco ...
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bioRxiv - Immunology 2022Quote: Mouse HSCs were isolated by negative selection from 5-8 week old female Balb/C mouse bone marrow using EasySep™ mouse hematopoietic progenitor cell isolation kit (Stemcell Technologies) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng ml−1) and PDGF-BB (10 ng ml−1; Shenandoah) and heparin (2 ng ml−1; Stem Cell Technologies). For all patient-derived cultures ...
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bioRxiv - Cell Biology 2022Quote: Bone marrow-derived GMPs from C57BL/6J mice were isolated by FACS sorting and cultured in Methocult GF M3434 (03434, Stem Cell Technologies) for 14 days ...
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bioRxiv - Cancer Biology 2022Quote: Tumours and matched lung metastases were dissociated into single cells using mechanical disruption followed by enzymatic digestion by a collagenase-hyaluronidase solution (StemCell Technologies; 07912) for 1 h at 37°C without mechanical agitation ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor tissue was processed by a member of the Precision Pathology Biobanking Center (PPBC) (55) and stored at room temperature in HypoThermosol FRS (Stem Cell Technologies) until implantation into the host mouse ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were prepared from buffy coats of healthy blood donors (Guangzhou Blood Center, Guangzhou, China) using Lymphoprep™ (STEMCELL Technologies) density gradient centrifugation according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Peripheral blood or the full contents of ABS:hematopoietic progenitor co-culture 24- wells were plated into complete methylcellulose media (Stem Cell Technologies, M3434). Colonies were scored 7-14 days after plating.
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bioRxiv - Neuroscience 2022Quote: ... The number of cells in the suspension was estimated with use of a cytometer and appropriate volume of suspension was added to AggreWell 800 (Stem Cell Technologies) that would result in 5000 cells per microwell being produced ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from lymph nodes of C57BL/6 Rag1-/+ OT-1-/+ mice and purified using the EasySep™ mouse CD8+ T cell isolation kit (STEMCELL Technologies) by negative selection ...
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bioRxiv - Cancer Biology 2022Quote: ... Naïve CD8+ T cells were harvested from CD1 wildtype P25 mice and processed using the EasySep™ Mouse Naïve CD8+ T Cell Isolation Kit (Stemcell Technologies, 19858). Following isolation ...
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bioRxiv - Cell Biology 2024Quote: ... 200-500 µm EBs were picked under a dissecting microscope and transferred onto adherent tissue culture plates (2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL2 (Stem Cell Tech 05270), with 0.5 % Protein free hybridoma medium (ThermoFisher Scientific ...