Labshake search
Citations for Stemcell Technologies :
4301 - 4350 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Isolated OSNs were collected from supernatants via centrifugation and resuspended in cell sorting medium of 1x PBS (diluted from commercial 10x PBS, pH 7.4) and BrainPhys Neuronal Medium (Stemcell Technologies). Initially ...
-
bioRxiv - Immunology 2020Quote: ... line (available from the European Collection of Authenticated Cell Cultures (karyotyped, passage 24-36)) was cultured on plates coated with 40µl/ml vitronectin in PBS (StemCell Technologies). E8 (Gibco ...
-
bioRxiv - Developmental Biology 2021Quote: ... colonies were disaggregated into single cells using Accutase and plated in the presence of Rock Inhibitor (StemCell Technologies Y-27632) on matrigel-coated plates ...
-
bioRxiv - Cell Biology 2020Quote: ... WTC CRISPRi GCaMP and WTC CRISPRi SIX3-g2 hiPSC lines were maintained on Vitronectin XF (Stem Cell Technologies, Cat.#07180) coated plates.
-
bioRxiv - Microbiology 2020Quote: ... Cells were differentiated under air-liquid interface (ALI) in PneumaCult-ALI medium containing hydrocortisone and heparin supplements (STEMCELL Technologies, 05001) as specified by the manufacturer’s guidelines ...
-
bioRxiv - Cancer Biology 2021Quote: CD34+ cells isolated from MF patients were plated at a density of 500 cells/replicate in 30-mm dishes containing 1 mL of StemSpan SFEM with 1.1% methylcellulose (Cat# H4230, StemCell Technologies), to which SCF ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells with the highest mNeonGreen signal were isolated to enrich for homozygous insertions and plated onto H4100 Methylcult (StemCell Technologies) to isolate clonal populations.
-
bioRxiv - Developmental Biology 2022Quote: ... All WTC CRISPRi and WTC CRISPRi HOPX g1 hiPSC lines were maintained on Vitronectin XF (Stem Cell Technologies, Cat.#07180) coated plates.
-
bioRxiv - Physiology 2022Quote: ... at an optimised density of 0.8-1.3×106 cells/well in mTeSR1 supplemented with 10 µM Y-27632 (StemCell Technologies, UK). Once cells had attached (6+ hours) ...
-
bioRxiv - Immunology 2022Quote: ... CD19+ cells were purified from C57BL/6 wild-type mice using the EasySep magnetic separation system (Stemcell Technologies, Vancouver, Canada) and were activated with lipopolysaccharide (10 μg/mL ...
-
bioRxiv - Neuroscience 2022Quote: ... hiNCs and hiACs were combined at a 1:1 ratio in Asteroid Media (DMEM/F12 (Stem Cell Tech cat#36254), 1% Glutamax (Thermo Scientific cat#35050061) ...
-
bioRxiv - Immunology 2019Quote: ... The pluripotency of the LSK cells isolated were confirmed using Colony-Forming Unit Assay according to the manufacturer’s instruction (STEMCELL Technologies). The LSK cells were rested overnight in SFEM medium (STEMCELL Technologies ...
-
bioRxiv - Genomics 2020Quote: ... Mononuclear cell subsets were obtained by isolation of PBMCs by gradient centrifugation in SepMate tubes (STEMCELL Technologies; cat. no. 85460), with Ficoll-Paque PLUS (GE Healthcare Life Sciences ...
-
bioRxiv - Neuroscience 2021Quote: ... Transfected cells were plated in 2 wells of a 6-well plate with mTeSR1 media supplemented with CloneR (STEMCELL Technologies) to enhance cell survival ...
-
bioRxiv - Bioengineering 2021Quote: ... Isolation of hematopoietic lineage negative was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA). For cytotoxicity studies ...
-
bioRxiv - Cancer Biology 2020Quote: ... and the cells then dissociated in 2.5 mg/ml trypsin with 1 mM EDTA and 5 mg/ml dispase (STEMCELL Technologies) with 100 μg/ml DNaseI (Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... or total T cells using EasySep isolation kits (Catalog Nos. 19765, 19853, and 19851; STEMCELL Technologies Inc., Vancouver, BC, Canada). Negative selection was confirmed by flow cytometry analysis to > 90% purity ...
-
bioRxiv - Genomics 2021Quote: Wild type SB AD3.1 [21] and AD/N3HAV lines were maintained as undifferentiated hiPSC in mTeSR1 medium (Stem Cell Technology) on 1:30 diluted Matrigel (hESC grade ...
-
bioRxiv - Genomics 2021Quote: ... Cells were split every 3 or 4 days with TrypLE Select (Fisher) and seeded at 1.5-4×10e5 in a Matrigel-coated p35 plate containing 5μM Y27632 (Stem Cell Technologies) (mTeSR+Y ...
-
bioRxiv - Genomics 2021Quote: ... For crypt isolation the intestinal tissue pieces were transferred into 10 ml of Gentle Cell Dissociation Reagent (07174, STEMCELL Technologies) and incubated for 15 mins at room temperature (RT) ...
-
bioRxiv - Cancer Biology 2021Quote: WT29 iPSCs and their derivatives were cultured on Laminin (Biolaminin 521 LN; Biolamina #LN521) in mTeSR1 (Stem Cell Technologies; # 85850) plus 1% Penicillin-Streptomycin (Thermo Fisher #15140122) ...
-
bioRxiv - Developmental Biology 2021Quote: ... naïve p7 that had been previously converted to the naïve stem cell state using NaïveCultTM induction media (Stemcell Technologies; 05580) were thawed onto high-density mouse (ICR ...
-
bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood from healthy donors (as mentioned above) by negative selection using RosetteSep (STEMCELL Technologies) then separated using a ficoll gradient ...
-
bioRxiv - Immunology 2021Quote: Naïve CD8 T cells were isolated from young female (6w-8w) B6 mouse lymph nodes and spleens with the EasySep Mouse Naïve CD8 T Cell Isolation Kit (StemCell Technologies) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: Both primary and BCi-NS1.1 airway epithelial cells were first expanded in Pneumacult-Ex or Pneumacult-Ex Plus medium (#05008, #05040; StemCell Technologies), and differentiated in Pneumacult-ALI medium (#05001 ...
-
bioRxiv - Immunology 2020Quote: Total CD4 T cells from donor mice were purified from spleens using STEMCELL EasySep CD4 T Cell Isolation Kit according to manufacturer’s instruction (STEMCELL Technologies). Depending on individual experiment ...
-
bioRxiv - Bioengineering 2020Quote: ... Initial hematopoietic lineage negative enrichment was performed with EasySep™ Mouse Hematopoietic Progenitor Cell Isolation Kit (#19856, Stemcell Technologies, CA), followed by collection of the Lin- Sca-1+ c-kit+ (LSK ...
-
bioRxiv - Genomics 2020Quote: NSTcPA buffer (1 mL) was pipetted into one well of a 6-well plate (Stem Cell Technologies, cat. no. 38015) on ice ...
-
bioRxiv - Neuroscience 2020Quote: ... Half of the medium was replaced by BrainPhys™ Neuronal Medium (supplemented with SM1 supplement; Stem cell technologies, Vancouver, Canada) every seven days.
-
bioRxiv - Neuroscience 2022Quote: ... NSPC cultures were then passaged every 7 days by enzymatic disaggregation with Accutase (cat# 7920, Stem Cell Technologies, Vancouver, Canada). The medium was supplemented with Neurocult proliferation supplement (cat# 05702 ...
-
bioRxiv - Immunology 2022Quote: ... LSK cells and GMPs were cultured in S-Clone SF-O3 (Sanko Junyaku) supplemented with 0.1% BSA (093001; STEMCELL Technologies), 50 μM 2-ME (Sigma-Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... Het and HO mice using EasySep B cell kits by negative selection according to the manufacturer’s instructions (Stemcell Technologies, Canada). B cells were stained with anti CD21-FITC and anti CD23-PE antibodies (Biolegend ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were maintained in NbM for 6 days after which they were passaged with 1 U/mL Dispase (StemCell Technologies) and cultured in NbM supplemented with 0.1 µM Retinoic Acid (RA ...
-
bioRxiv - Synthetic Biology 2023Quote: iPS11 hiPSCs (Alstem Cell Advancements, Richmond, CA, USA; iPS11) cultured with mTeSR-Plus (StemCell Technologies, Vancouver, BC, Canada; 100-0275) according to the manufacturer’s protocol on GelTrex-coated plates (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: ... lymph nodes and spleen from 16-24-week-old female or male C57Bl/6-Foxp3gfp × Thy1.1 mice were collected and CD4+ T cells isolated by negative selection (STEMCELL Technologies). Tregs were sorted as live CD4+CD8-Thy1.1+Foxp3gfp+ using a MoFlow® Astrios (Beckman Coulter ...
-
bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 1.25×5 cells/cm2 on Matrigel hESC-qualified Matrix-coated wells and maintained in Neural Progenitor medium (StemCell Technologies) with daily complete media change ...
-
bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were resuspended in StemSpan™ SFEM II supplemented with StemSpan™ CD34+ Expansion Supplement (10X) (StemCell Technologies, Catalog # 09720). Cells were plated on 24-well non-treated plates at a density of 100,000 cells/mL and incubated at 37°C for 2-3 days.
-
bioRxiv - Bioengineering 2024Quote: ... at the density of 4.2×104 cells/well and add PneumaCult™-Ex Plus Medium (STEMCELL Technologies, Cat. No. 05040) to both the basal and apical chambers ...
-
bioRxiv - Cancer Biology 2024Quote: ... Peripheral Blood Mononuclear Cells (PBMCs) were isolated from healthy donor’s blood using density gradient centrifugation with Lymphoprep™ (STEMCELL Technologies) as the separation medium ...
-
bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
-
bioRxiv - Cell Biology 2024Quote: FACS-sorted GFPhi MAPs from mammary glands of PdgfrαH2B-eGFP mice were seeded onto collagen-coated wells of a 24-well plate at 20,000 cells per well and cultured in DMEM media with 10% FBS and 10ng/ml bFGF (STEMCELL Technologies). Following 5 days of culture ...
-
bioRxiv - Neuroscience 2024Quote: ... APCs were seeded onto Matrigel coated 6 well plates at 200,000 cells per well with STEMdiffTM Astrocyte Maturation medium (STEMCELL Technologies). After 2 weeks ...
-
bioRxiv - Developmental Biology 2024Quote: ... for 10 min at 37° C and seeded on to Geltrex-coated 12-well plates at a density of 700,000 cells in neural induction medium + 10 uM Y-27632 (Stemcell Technologies) and cultured for 7 days with daily medium exchange ...
-
bioRxiv - Developmental Biology 2024Quote: ... cells were dissociated into single-cell suspension using Accutase and re-seeded onto fresh Poly-L-Ornithine / Laminin coated 12-well plates at a density of 150,000 cells in STEMdiff Neuron Maturation Kit medium (Stemcell Technologies) and medium exchanged every other day until days 14 and 21.
-
bioRxiv - Immunology 2024Quote: ... Freshly isolated HSPC populations (LT-HSC, ST-HSC, MPP, CLP, CMP, MDP) were resuspended in StemSpan medium (Stem Cell Technologies) supplemented with 50 ng/mL stem cell factor (SCF ...
-
bioRxiv - Cell Biology 2023Quote: CFU assay was performed at the indicated time points plating 800 cells in methylcellulose-based medium (MethoCult H4434, StemCell Technologies) supplemented with penicillin and streptomycin ...
-
bioRxiv - Genetics 2023Quote: The generated iPSCs were differentiated into three germ layers using the STEMdiffTM Trilineage Differentiation Kit (Stem Cell Technologies Catalog #05230).
-
bioRxiv - Developmental Biology 2023Quote: ... and supplied with Basal culture medium consisting of DMEM/F12 (with 15 mM HEPES) (Catalog no. 36254, Stem Cell Technologies), 1x GlutaMAX (Catalog no ...
-
bioRxiv - Genetics 2023Quote: The differentiation of hESCs toward beating cardiomyocyte was performed following STEMdiffTM Ventricular Cardiomyocyte Differentiation Kit (Stem Cell Technologies Catalog #05010) in accordance with the manufacturer instructions ...