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Citations for Stemcell Technologies :
4501 - 4550 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: For cell growth assay 100 LIN-CD34+CD38- CD9+ or CD9- cells were sorted and seeded in 96 well plate and culture in StemSpan Serum –Free Expansion Medium (SFEM, StemCell Technologies) supplemented with 100u/ml penicillin (Hyclone ...
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bioRxiv - Microbiology 2023Quote: ... splenocytes were fused with myeloma cells P3X63Ag8 (ATCC, France) using ClonaCell-HY Hybridoma Kit according to manufacturer’s instructions (StemCell Technologies, Canada). Culture supernatants were screened using ELISA (see below ...
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bioRxiv - Microbiology 2024Quote: ... After 4 days the monolayers of cells were subsequently maintained in an air-liquid environment with differentiation media (PneumaCult-ALI medium from STEMCELL Technologies) added to the lower (basolateral ...
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bioRxiv - Molecular Biology 2024Quote: ... GBOs cultured for more than one month were dissociated into a single cell with accutase at 37 °C for 30 min and seeded into aggrewell 800 (Stemcell technologies) at a density of 9 × 105 cells/mL with 1 mL of GBO medium supplemented with 10 µM Y27632 ...
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bioRxiv - Immunology 2024Quote: The M210B4 stromal cell line was isolated from normal mouse bone marrow adherent cells50 and maintained in RPMI 1640 medium (STEMCELL Technologies) with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were isolated from spleens of P14 donor mice by negative selection using the EasySepTM Mouse CD8+ T-cell isolation kit (STEMCELL Technologies). P14 cells were activated in vitro for 24-28 hours with 100U/mL recombinant IL-2 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were resuspended and 50,000-100,000 cells were transferred into fresh matrix-coated plates with mTeSR Plus media containing 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). Fresh media without ROCK inhibitor was fed the next ...
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bioRxiv - Developmental Biology 2024Quote: ... A 1-5 min centrifugation of the well-plate was performed at 200g and cells in suspension were cultured for one day in mTesR Plus (StemCell Technologies) to allow for aggregation and Embryoid Body (EB ...
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bioRxiv - Immunology 2024Quote: ... n=1 female) mice were prepared by depleting CD4+ cells from thymocytes using the EasySepTM Mouse CD4 Positive Selection Kit II (STEMCELL Technologies). Cells were then incubated with Fixable Viability Dye eFluor™ 780 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from blood of convalescent COVID-19 donors using the EasySep B kit (Stemcell Technologies, cat no 17954). SARS-nCoV-2 Spike RBD (Raybiotech ...
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bioRxiv - Bioengineering 2023Quote: ... splenic T lymphocytes were isolated from Cas9 transgenic mice using negative selection with the EasySep Mouse T Cell Isolation Kit (Stemcell Technologies) and then activated with Dynabeads Mouse T-Activator CD3/CD28 beads (Cat # 11456D ...
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bioRxiv - Bioengineering 2024Quote: ... HSPCs were thawed and cultured at a concentration of 5×105 cells/ml in the “HSPC medium” containing StemSpan (STEMCELL Technologies) supplemented with penicillin/streptomycin (Gibco) ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were dissociated into single cells using Accutase and plated on 10 µg/mL Vitronectin XF (Cat. No. 07180, STEMCELL Technologies)-coated plates with 10 µM of Y27632 (Cat ...
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bioRxiv - Microbiology 2023Quote: ... in complete growth media from LifeLine Cell Technology (LL-0023) and subsequently differentiated to air with complete Pneumacult ALI media (05001) from Stemcell Technologies. Cells were incubated for approximately two weeks until appearance of ciliated cells and mucus production was seen (53) ...
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bioRxiv - Biophysics 2023Quote: NK-92 (ATCC®, CRL-2407™) were cultured in the ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies Inc., USA) with the addition of Human Recombinant Interleukin 2 (IL-2 ...
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bioRxiv - Microbiology 2023Quote: ... A final boost in PBS was administered and 4 days later splenocytes were fused with Sp2/10 Ag14 cells (ATCC CRL-1581) and cultured overnight prior to concurrent cloning and HAT selection on methylcellulose plates (ClonaCell-HY, StemCell Technologies). After 14 days of incubation at 37°C ...
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bioRxiv - Neuroscience 2023Quote: Patterned differentiation of iPSCs toward neural progenitor cells (NPCs) was conducted using dual-SMAD inhibition (STEMdiff SMADi Neural Induction Kit, STEMCELL Technologies). To promote complete and uniform generation of NPCs ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 x 104 sorted cells were plated in triplicate in serum-free media containing StemSpan™ Megakaryocyte Expansion Supplement (STEMCELL Technologies) for 10-12 days ...
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bioRxiv - Cancer Biology 2023Quote: Day 5-18 differentiated cells were dissociated with Accutase for 10 min and CD34+ population was purified by magnetic-activated cell sorting (MACS) with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Daily media changes were performed for seven days followed by passaging of progenitor cells using Accutase (Cat No. 07920, STEMCELL Technologies), which was repeated for one additional passage (two passages total) ...
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bioRxiv - Cancer Biology 2023Quote: The ALDH assay was performed in accordance with the manufacturer’s instructions for the ALDEFLUOR TM kit (Stem Cell Technologies, Cat # 01700). Cells were trypsinized ...
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bioRxiv - Immunology 2023Quote: ... CD8α+ cells were purified from CD4 and CD8β depleted T-IEL and lymph node T cells using the CD8α Positive Selection Kit (Stemcell Technologies). Cells were resuspended in RPMI + 1% FBS + L-Glutamine + Pen/Strep at a concentration of 1x106 cells/ml and stimulated with 10 µM of pervanadate ...
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bioRxiv - Cell Biology 2023Quote: ... and sub-plated at 25 x 104 cells/ml on dishes coated with 50 μg/ml poly-L-ornithine in BrainPhys neuronal medium (STEMCELL Technologies) containing N2 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270 ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were first differentiated into hematopoietic progenitor cells (HPCs) following the manufacturer’s instructions using the commercially available STEMdiff Hematopoietic Kit (Stemcell Technologies, 05310). HPCs in suspension were transferred to plates containing an adherent layer of confluent primary human astrocytes (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: CD8+ T cells were isolated from the spleens and lymph nodes of OTI mice using the EasySep Mouse CD8+ T Cell Isolation Kit (StemCell Technologies). For direct co-culture experiments ...
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bioRxiv - Microbiology 2023Quote: ... cells were seeded at an initial density of 25000 cells/transwell insert and cultured in submerged culture using Pneumacult-EX medium (Stemcell Technologies) until confluence was reached ...
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bioRxiv - Cell Biology 2023Quote: ... 2×105 freshly isolated cells from digests of aorta or specified tissue were plated in methylcellulose (MethoCult GF M3434, StemCell Technologies), in duplicate ...
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bioRxiv - Immunology 2023Quote: ... was added into the dissociated CO/PCCO single cells suspension together with 1 µL Biotin selection cocktail (Annexin V, STEMCELL Technology), gently mixed and incubated at RT for 3 min ...
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bioRxiv - Immunology 2023Quote: ... and purified CD4+ T cells were obtained by positive selection with the “EasySep Mouse CD4 Positive Selection II” kit (StemCell Technologies). Then ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were plated on a Matrigel or Culturex coated culture plate and cultured in 10 uM Y27632 (72304, StemCell Technologies) containing mTeSR1 one day before differentiation ...
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bioRxiv - Cell Biology 2022Quote: ... were obtained from the CCHMC Pluripotent Stem Cell Facility (PSCF) (44) and cultured in feeder-free condition with mTeSR1 (85850, StemCell Technologies) and on a hESC-qualified Matrigel (354277 ...
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bioRxiv - Cancer Biology 2023Quote: ... A total volume of 10-20 ml of fresh blood was collected in EDTA separator tubes and peripheral blood mononuclear cells (PBMC) were immediately isolated using Lymphoprep and SepMate-50 (Stemcell Technologies) centrifugation tubes ...
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bioRxiv - Genomics 2023Quote: ... human iPSCs were plated at cell density of 3.5×105 cells/well on Matrigel coated 12-well-plates using mTeSR™ medium supplemented with 5µM ROCK inhibitor (Y-27632, STEMCELL Technologies) for 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... A single-cell suspension was obtained by incubating the dissected embryos at 37°C in a dissociation solution consisting of accutase (Stemcell Technologies) with 3U/mg Papain (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... Intestinal crypts were mechanically released from the lamina propria by vigorous pipetting and washed using PBS for twenty times followed by incubating in 25 mL gentle cell dissociation reagent (STEMCELL Technology) for 15min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... hiPSCs cultured in mTeSR1 were used for encapsulation in alginate hydrogels at 70% confluency as single cells using Accutase (STEMCELL Technologies) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... UCB mononuclear cells (UCBMC) and plasma were isolated after whole-blood centrifugation over LymphoPrep in SepMate tubes (STEMCELL Technologies, Vancouver, BC) following manufacturers’ protocols ...
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bioRxiv - Cell Biology 2023Quote: ... triturated with a 1000 µl pipette and dissociated in a collagenase solution with 1 U of Dispase (Stem Cell Technologies, 07923), 2 mg/ml Collagenase IV (Worthington Biochemical Corporation ...
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bioRxiv - Genomics 2023Quote: Whole blood samples of 5-10 mL were collected in EDTA Vacutainer test tubes and processed for the isolation of erythroid progenitor cells (EPCs) using the Erythroid Progenitor Kit (StemCell Technologies). EPCs were reprogrammed using Sendai particles (CytoTune-iPS 2.0 Reprogramming kit ...
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bioRxiv - Molecular Biology 2023Quote: ... Double-positive cells were plated into a matrigel-coated 6 cm petri dish in mTeSR supplemented with 1x CloneR (Stemcell Technologies). Individual colonies were manually picked into 96 wells ∼10 days after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Minced tissues were transferred with help of a sterile wide bore 200 µl pipette tip into a 1.8 ml cryo vial containing 1.2 ml A-mTE-D1 (300 IU ml-1 collagenase IV mixed with 100 IU ml-1 hyaluronidase; Stem Cell Technologies, 07912 ...
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bioRxiv - Cancer Biology 2023Quote: ... and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Immunology 2023Quote: ... the spleen of the mouse with the highest antibody titers was dissociated and fused with Sp2/0-Ag14 myeloma cells (ATCC) using the ClonaCell-HY Hybridoma Kit (StemCell Technologies) according to the manufacturer’s protocol ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Blood samples from patient CIII were collected by Phenocell SAS (Grasse, France) and peripheral blood mononuclear cells (PBMC) were freshly collected using the SepMate (Stemcell Technologies) protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The genetic integrity of the engineered hiPSC clones was evaluated by a qPCR-based hiPSC genetic analysis kit (Stem Cell Technologies).
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bioRxiv - Neuroscience 2023Quote: ... To confirm Treg depletion spleen and lymph nodes were mashed through a 70 μm strainer and then treated with red blood cell lysis buffer (STEMCELL Technologies) for 2 min at room temperature ...