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Citations for Stemcell Technologies :
4201 - 4250 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Aldefluor assay was performed to determine aldehyde dehydrogenase (ALDH) enzymatic activity using Aldefluor kit (Stem Cell Technologies, Catalog #01700) following manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized anti-coagulated blood using density gradient Lymphoprep medium (Stemcell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ T cells were pre-activated with either CD3/CD28/CD2 tetrameric antibody complexes (ImmunoCult by STEMCELL Technologies) or 1:1 aAPCs for 5 d ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... cells were cultured in Mesencult proliferation medium (Mesencult Basal Medium supplemented with MSC stimulatory supplements (StemCell Technologies, Grenoble, France) and 2% antibiotics and glutamine) ...
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ HSPCs were cultured at 2.5×105–5×105 cells/mL in StemSpan SFEM II (STEMCELL Technologies, Vancouver, Canada) base medium supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Immunology 2021Quote: ... Bulk T cells were isolated from Leukopaks using EasySep magnetic selection following manufacturers’ recommended protocol (Stemcell Technologies cat 17951). Immediately following isolation ...
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bioRxiv - Cell Biology 2021Quote: ... tissue digests were thawed and placed in PBS containing 0.1mg/ml DNAse I solution (Stem Cell technologies, Cat#07900) to digest DNA released from dead cells ...
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bioRxiv - Bioengineering 2021Quote: ... the CE and Descemet’s Membrane were manually stripped with forceps and incubated in Dispase (1U/mL, Stem Cell Technologies) for 1.5 hours at 37°C to detach the rabbit CE cells (RCECs ...
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bioRxiv - Cancer Biology 2021Quote: ... Following enzymatic digestion of tumor tissues using 1x Collagenase/Hyaluronidase and DNase-1 (Cat # 07912 & 07900; Stem cell technologies), dissociated tumor cells were collected in a different tube and filtered through 40 µ strainer ...
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bioRxiv - Immunology 2022Quote: ... Mononuclear cells (MNCs) were enriched using Lymphoprep density gradient medium (Catalog #07811) and SepMate tubes (Catalog #85450; STEMCELL Technologies). Briefly ...
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bioRxiv - Immunology 2022Quote: ... naïve CD8+ T cells were purified from spleen and peripheral lymph nodes by negative selection (StemCell Technologies, Vancouver, BC) as previously described (2) ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were isolated from splenocytes of OT-I mice using a magnetic bead isolation kit (Stemcell Technology) and cultured following a previously published protocol (44) ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... Tet2 +/− and Tet2−/− mice were purified by flow cytometry and plated in methylcellulose medium (Methocult M3434, Stem Cell Technologies). Total number of colony-forming units (CFU ...
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bioRxiv - Cancer Biology 2022Quote: ... We grew cells in a 5% CO2 incubator in a serum-free NeuroCult NS-A basal medium (STEMCELL Technologies) supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Red blood cells were removed using the ammonium chloride solution: 5 ml of 0.8% Ammonium Chloride (StemCell Tech. 07800) was added to the suspension and incubated for 10 min with intermittent mixing ...
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bioRxiv - Cell Biology 2022Quote: ... 800ul of cleared media or cell extracts were mixed with 200ul of OptiPrep Density Gradient Medium (Stemcell Technologies: 7820). 900ul of the mixture were transferred to a polycarbonate ultracentrifuge tubes (Beckman Coulter:343778) ...
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bioRxiv - Immunology 2022Quote: ... Approximately 45mg of each frozen liver sample was placed in a 6-well tissue culture plate (Stem Cell Technologies) with 1 ml TST (2 mL of 2X ST buffer + 120 µL of 1% Tween-20 + 20 µL of 2% BSA brought up to 4 ml with nuclease-free water) ...
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bioRxiv - Neuroscience 2023Quote: ... The Plating medium was consisted of 1 × BrainPhys Neuronal Medium/N2-A/SM1 kit (BrainPhys; Stem Cell Technologies, 05793) supplemented with 10 μM Y27632 ...
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bioRxiv - Neuroscience 2022Quote: Blood mononuclear cells were isolated from venous blood draws from healthy volunteers and processed with SepMate (Stemcell Tech, Inc.) tubes and Lymphoprep (Stemcell Tech ...
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bioRxiv - Neuroscience 2022Quote: ... iPSCs were expanded in colonies on a Matrigel layer in mTeSR1 Embryonic stem cell media (StemCell Technologies, cat.no. 85850) as directed by batch instructions from the manufacturer ...
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bioRxiv - Bioengineering 2024Quote: HSPCs were plated at a concentration of 1 × 103 cells/ml in a methylcellulose-containing medium (GFH4435, STEMCELL Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were initially plated on 10% Matrigel coated plates and expanded in PneumaCult™-Ex Plus Medium (Stemcell Technologies) + 1x PneumaCult™-Ex Plus Supplement (Stemcell Technologies ...
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bioRxiv - Immunology 2024Quote: ... cells harvested from the LEM cultures or xenografts were washed twice in Dulbecco’s phosphate-buffered saline (PBS, STEMCELL Technologies) to remove residual serum ...
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bioRxiv - Developmental Biology 2024Quote: ... resuspended in 100 μL phosphate buffered saline (PBS) without Ca2+ and Mg2+ (D-PBS; Stem Cell Technologies, cat.# 37350) and 20 μL aliquots were prepared and stored at -80°C.
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bioRxiv - Immunology 2024Quote: ... with 5×103 splenic P14 TN cells congenic for CD90.1+ or CD45.1+ isolated by negative selection using the EasySep Mouse CD8+ T cell isolation kit (STEMCELL Technologies) before LCMV Arm infection eight hours later ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended at a concentration of 1×106 cells/mL in neuron media [Brainphys (StemCell Technologies #05790), 1×N2 (ThermoFisher #17502-048) ...
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from healthy donors or patients via centrifugation with lymphoprep (07851, StemCell Technologies) and SepMate conical tubes (85450 ...
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bioRxiv - Microbiology 2023Quote: Phosphorylation status of p65 and c-JUN were analyzed by flow cytometry in isolated CD4 T cells (StemCell Technologies) as previously described (44) ...
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bioRxiv - Developmental Biology 2023Quote: ... we cultured the 3dpf embryos for 24h in the same medium supplemented with 0.5 µM reversine (Stem Cell Technologies) and after wash-out we let them develop until 5dpf and subsequently took a biopsy (Fig ...
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bioRxiv - Developmental Biology 2023Quote: ... 500,000 cells were seeded into each well of AggreWell™800 24-well Plate (STEMCELL Technologies, Cat. No. 34850) with 2 mL mNPSR-v2 medium and cultured overnight ...
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bioRxiv - Genetics 2023Quote: ... and fed every other day with stem cell media: mTeSR Plus with supplement (STEMCELL Technologies; cat. no. 100-0276) and 0.5% penicillin-streptomycin (ThermoFisher ...
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bioRxiv - Genetics 2023Quote: ... The medium was changed every day and cells were passaged when 70% confluent using ReleSR (STEMCELL Technologies, #100-0484) at a 1:20 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... after hiPSC colonies reached 70-80% confluency in feeder independent conditions in mTESR medium (Stem Cell Technology, cat. # 85850), they were detached with Accutase (Stem Cell Technology ...
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bioRxiv - Bioengineering 2023Quote: ... The following day cells were infected with lentivirus containing a doxycycline-inducible Ngn2 cassette in mTeSR media (StemCell Technologies) containing polybrene (10 mg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... and re-plated at 12,000 cells per well into Cultrex-coated 96-well plates with mTeSR media (StemCell Technologies) containing ROCK inhibitor Y-27632 (10 uM ...
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bioRxiv - Genetics 2023Quote: ... all plasticware used to handle the cerebral organoids was pretreated with an anti-adhesion solution (Stem Cell Technology #07010). All fixation steps described in this study were performed using 4% paraformaldehyde (PFA ...
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bioRxiv - Genomics 2023Quote: ... Neural rosettes were harvested by treating 14-day-old EBs with STEMdiff Neural Rosette Selection Reagent (Stem Cell Technologies) for 60 minutes at 37°C and were then plated onto polyornithine/laminin- coated plates and cultured with NPC medium containing DMEM/F-12 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD11B-FITC (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Immunology 2023Quote: ... The cells at the interface were collected and cultured in poly-HEMA-coated flasks with StemSpan SFEM (STEMCELL Technologies) supplemented with lipid mixture 1 (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... and residual natural killer cells were depleted using 100 μL/mL of the CD56 Positive Selection Cocktail (STEMCELL Technologies). Monocytes (5ξ106/vial ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were either used fresh for ex vivo experiments or preserved in CryoStor® CS10 freezing media (STEMCELL Technologies). For in vivo experiments ...
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bioRxiv - Genetics 2023Quote: ... and fed every other day with stem cell media: mTeSR Plus with supplement (STEMCELL Technologies; cat. no. 100-0276) and 0.5% penicillin-streptomycin (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2) at a cell concentration of 5 × 105/mL in StemSpan Serum-Free Expansion Medium II (STEMCELL Technologies) supplemented with 100 ng/mL of human recombinant SCF ...
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bioRxiv - Immunology 2023Quote: ... APCs from NOD spleens were obtained by depleting T cells using the Mouse CD90.2 Positive Selection Kit (STEMCELL Technologies) then pulsed with 15 µM of the indicated peptides and co-cultured with Tregs/Tconvs at a 1:1 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... cells were passaged and seeded as above and cultured in complete STEMdiffTM Astrocyte Maturation Medium (AMM) (Stemcell Technologies, #08550) with medium changed every other day ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were washed twice with PBS (+ 2%FBS) and with a red blood cell lysis buffer (0.8% NH4Cl, 0.1mM EDTA, STEMCELL technologies). Thereafter ...
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bioRxiv - Molecular Biology 2023Quote: ... hPSC-generated NPCs (passage 1) were maintained and propagated using complete STEMdiff™ Neural Progenitor Medium (Stem Cell Technologies). Cells from passages 1 to 6 were cryopreserved.
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bioRxiv - Cell Biology 2023Quote: ... Cells were plated immediately into pre-warmed antibiotic-free HSPC media (StemSpan SFEM II; Stemcell Technologies; cat. no., 09605_C) supplemented with SCF (100 ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and cultured overnight in mTeSR Plus medium supplemented with 10 µM Y-27362 ROCK inhibitor (Stem Cell Technologies, Canada). Differentiation was induced on day 0 by first washing cells with PBS when the monolayer reached approximately 80% confluence ...