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Citations for Stemcell Technologies :
4451 - 4500 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... All cell lines were cultured in humidified incubators (37 C and 5% CO2) using TeSR-E8 media (StemCell Technologies, Vancouver, Canada) or STEMdiff Neural Induction Medium (StemCell Technologies ...
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bioRxiv - Genomics 2020Quote: ... cells were counted using an automated cell counter and 900,000 iPSCs were re-suspended in 15ml of mTeSR medium (Stemcell Technologies 85850) with 50μM ROCK inhibitor (Santa Cruz sc-216067A) ...
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bioRxiv - Immunology 2020Quote: ... after CD3+ T cell depletion using the EasySep Human CD3 Positive Selection Kit II and the and the protocol for Using EasySep Positive Selection Kits for Cell Depletion provided by the manufacturer (STEMCELL Technologies) or after NK cell enrichment using the EasySep Human NK Cell Enrichment Kit (STEMCELL Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMCs) were collected from whole blood samples of healthy donors using SepMate™ tubes (STEMCELL Technologies, Canada) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Day 8 differentiated Ngn2 neurons were re-seeded at a density of 1×105 cells per 100 µL droplet in CM2 media consisting of BrainPhys (STEMCELL Technologies), 1x penicillin/streptomycin ...
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bioRxiv - Genetics 2020Quote: BM MNCs cells were seeded on 35-mm culture dishes in triplicate in methylcellulose medium supplemented with cytokines (MethoCult GF M3434, STEMCELL Technologies). CFU-Cs (colony forming units in culture ...
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bioRxiv - Developmental Biology 2020Quote: ... Both cell lines were routinely cultured on six-well plates coated with growth factor reduced Matrigel in mTeSR media (STEMCELL Technologies) with daily media replacement ...
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bioRxiv - Immunology 2021Quote: ... CD11b+ monocyte/macrophages were isolated using positive magnetic selection using the EasySep Mouse CD11b Positive Selection Kit II (Stem cell technologies), according to manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: Peripheral blood mononuclear cells (PBMCs) were separated from whole blood samples of healthy donors using SepMate™ tubes (STEMCELL Technologies, Canada) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... media was supplemented with Y27632, Laminin (2.5 µg per 1ml of media, Thermo-Fisher) and 0.1µM Compound-E (Stem Cell Technologies, Grenoble, France) during the first 3 days after plating in Poly-L-Lysine (Sigma ...
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bioRxiv - Cell Biology 2022Quote: CFU assays in PHZ-treated and retrovirally-infected spleen progenitors were conducted using spleen suspensions at 2 × 104 cells/mL mixed with Methocult M3434 (STEMCELL Technologies) containing Epo ...
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bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
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bioRxiv - Immunology 2020Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood using density gradient centrifugation with Lymphoprep in SepMate tubes (Stemcell Technologies) in a biosafety level 2+ facility according to the manufactory’s instruction ...
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bioRxiv - Cell Biology 2019Quote: ... The gathered crypts were centrifuged at 1200 rpm for five minutes and digested with dispase (1 U/ml, Stem Cell Technologies). The single cell suspension was passed through a 40µm cell strainer (BD biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... when they reached high confluency and re-plated in mTeSR 1 with RHO/ROCK Pathway Inhibitor Y27632 (Stem Cell Technologies, 72302). Patient donors are identified with a code with one numeral (DEL_1 ...
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bioRxiv - Cell Biology 2020Quote: ... H9 ESC (hESC) and hiPSC lines were grown in VTN coated plates in Essential-8 medium (TeSR-E8, Stem Cell Technologies), and dissociated with gentle cell dissociation reagent (0.5mM EDTA in PBS ...
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bioRxiv - Genomics 2020Quote: Kasumi-1-AE-wild-type or Kasumi-1-AE-FKBP cells were treated for 24 hours with 500 nM dTAG-47 prior to plating in methylcellulose (MethoCult H4434, StemCell Technologies). 500 cells were plated in 1.1 mL Methocult supplemented with 500 nM dTAG-47 ...
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bioRxiv - Immunology 2020Quote: ... infection efficiency was determined by GFP expression and infected cells were isolated using RoboSep mouse CD8a positive selection kit II (STEMCELL Technologies Inc. ...
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bioRxiv - Cell Biology 2020Quote: Peripheral blood was collected from a healthy volunteer into a BD Vacutainer tube and mononuclear cells were isolated using the SepMate kit (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Spleens of recipient mice were collected 8 days after viral infection and CD8+ T cells were purified by magnetic bead selection (EasySep, STEMCELL Technologies). Fluorescence-activated cell sorting is used for the separation of tdTomato OT-I CD8+ cells from the CD8+ T cells of recipient WT mice.
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Genomics 2021Quote: ... cells were cultured in StemMacs iPS-Brew media (Miltenyi Biotech, Auburn, CA) and routinely passaged utilizing Gentle Cell Dissociation Reagent (STEMCELL Technologies) onto Matrigel-coated (1 mg ml-1 ...
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bioRxiv - Genetics 2020Quote: ... to each well for 1h at room temperature) containing 0.5ml complete ReproTeSR medium (ReproTeSR basal medium with 25X ReproTeSR supplement) (Stem Cell Technologies). After 24h incubation ...
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bioRxiv - Genetics 2020Quote: Approximately 50,000 PBMCs were resuspended in complete Erythroid Medium (StemSpan-SFEM II with 100X StemSpan Erythroid Expansion Supplement) (Stem Cell Technologies) and transferred to a single well of a 6-well tissue culture plate for 24h ...
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bioRxiv - Cancer Biology 2020Quote: ... The MNCs were pre-cleared of T cells and Monocytes by consecutive immunomagnetic negative selection (CD3 and CD14-EasySep StemCell Technologies) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... CD90.2+ cells were pre-sorted from Rag2−/− lung and bone marrow single cell suspensions using an EasySep™ Mouse CD90.2 Positive Selection Kit II (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells from spleens were negatively enriched using an EasySep™ Mouse CD8+ T Cell Isolation Kit (#19853) according to the manufacturer’s instructions (STEMCELL Technologies). Enriched CD8+ T cells (92% - 96% purity ...
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bioRxiv - Neuroscience 2022Quote: ... Medium was replaced every other day and cells were passaged ~1:10 onto fresh Matrigel-coated vessels using ReLeSR (StemCell Technologies) when 90% confluent.
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bioRxiv - Cell Biology 2022Quote: 6×107 hiPS cells were mixed with lentiviral Dolcetto library to achieve estimated 0.3 MOI and seeded on 2x Vitronectin coated 5-layer flasks at 36K cells/cm2 density in mTeSR-Plus medium with Rock Inhibitor (Ri, 10μM, Y-27632, Stemcell technologies, 72304). The next day ...
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bioRxiv - Cancer Biology 2022Quote: CD34-positive and Lin-negative cells from PBMC were single-cell sorted into 96-well plates containing 100 μL of free medium consisting of StemSpam SFEMII (Stemcell Technologies) with human cytokines SCF ...
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bioRxiv - Bioengineering 2022Quote: ... or were cultured for 48 hours at a density of 2.5 × 105 cells/ml in StemSpan SFEM II (STEMCELL Technologies Inc.) supplemented with Stem Cell Factor (SCF ...
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bioRxiv - Cell Biology 2022Quote: ... then gently detached with Accutase and reseeded as 2-5×104 cells/cm2 in mTeSR medium containing 10uM Y-27632 (#72304, STEMCELL Technologies) ROCK inhibitor (removed after 48h and exchanged for fresh medium without ROCK inhibitor) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells were isolated from spleens and lymph nodes of P14 Tcf7GFP or OTI Tcf7GFP mice using the EasySepTM Mouse CD8+ T cell Isolation Kit (Stemcell Technologies), following manufacturer’s instructions.
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bioRxiv - Genetics 2022Quote: ... HSPCs were transferred into erythroid differentiation medium (EDM) consisting of StemSpan serum-free expansion medium II(SFEM II; Stem Cell Technologies) with 1% penicillin/streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2022Quote: ... Peripheral blood and bone marrow mononuclear cells were isolated using Ficoll Paque Plus solution and density gradient centrifugation using SepMate tubes (StemCell Technologies). All samples were stored in vapor-phase liquid nitrogen after cryopreservation with 10% dimethyl sulfoxide until analysis ...
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bioRxiv - Microbiology 2022Quote: ... media was removed from the apical surface and cells were fed basolaterally with Pneuma-Cult ALI (Air Liquid Interface) differentiation medium (STEMCELL TECHNOLOGIES) for 5-8 weeks to allow differentiation of cells at the air-liquid interface ...
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bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
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bioRxiv - Immunology 2022Quote: ... The cells were cultured on an air-liquid interface (ALI) for differentiation in culture media (PneumaCult ALI media, STEMCELL Technologies, Canada) to allow the progenitor cells to differentiate into a mucociliary airway epithelium ...
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bioRxiv - Neuroscience 2023Quote: ... sorted into 96-well plates using a WOLF benchtop microfluidic cell sorter (Nanocellect) in mTeSR Plus and CloneR (Stemcell Technologies, 05888). After thirteen days ...
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bioRxiv - Immunology 2022Quote: ... We used a method of MAIT cell expansion as previously described13 with the following modifications: instead of using base medium of Immunocult-XF T cell expansion medium (Stemcell Technologies) we used RPMI-1640 and substituted CTS serum (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: H9 human ES cell lines obtained from the HMS Cell Biology Initiative for Genome Editing and Neurodegeneration were cultured using the mTeSR1TM1 kit (STEMCELL technology) and 1% penicillin-streptomycin (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... The CHOP-WT10 cell line was maintained as an iPSC culture35 and subsequently transitioned to feeder-free cultures and maintained with mTeSR1 (StemCell Technologies) on hESC qualified matrigel (Corning) ...
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bioRxiv - Neuroscience 2022Quote: ... Briefly, iPSCs were cultured by StemFit (AK02 N, Ajinomoto) and were collected using Gentle Cell Dissociation Reagent (ST-07174, STEMCELL Technologies) when cells were confluent on six-well dishes ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured in feeder-free medium and grown on Matrigel-coated plates in cell medium containing 1:1 mTesR1: Essential 8 (StemCell Technologies, 85850 and Thermofisher A1517001 ...
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bioRxiv - Developmental Biology 2022Quote: ... From day 7 - 9 of differentiation cells were cultured in cSFDM containing 2 μM Retinoic acid and 0.5 μg/ml purmorphamine (Stemcell Technologies, #72202), and then subsequently in cSFDM with 2 μM Retinoic acid until day 11-13 of differentiation ...
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bioRxiv - Microbiology 2024Quote: ... After 4 days the monolayers of cells were subsequently maintained in an air-liquid environment with differentiation media (PneumaCult-ALI medium from STEMCELL Technologies) added to the lower (basolateral ...
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bioRxiv - Bioengineering 2024Quote: ... M2-10B4 (overexpressing IL-3 and G-CSF) and Sl/Sl (overexpressing IL-3 and SCF) were passaged at ∼90% confluence (cell lines gifted from StemCell Technologies). Cells were grown for 2 weeks prior to use in selection agents to select stromal cells expressing long-term cell maintenance factors (M2-10B4 ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were gently resuspended in media and transferred into 6-well plates in mTeSR Plus media containing CloneR2 reagent (STEMCELL Technologies) for AAVS1 targeting ...
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bioRxiv - Bioengineering 2024Quote: ... HSPCs were thawed and cultured at a concentration of 5×105 cells/ml in the “HSPC medium” containing StemSpan (STEMCELL Technologies) supplemented with penicillin/streptomycin (Gibco) ...