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Citations for Stemcell Technologies :
4101 - 4150 of 4852 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Peripheral blood mononuclear cells (PBMCs) were isolated from three healthy donors through SepMate density centrifugation (StemCell Technologies, 85450) with Ficoll-Paque PLUS (Cytiva 17-1440-03) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the hematopoietic progenitors were isolated by magnetic negative selection (Mouse Hematopoietic Progenitor Cell Isolation Kit, StemCell Technologies), and cultivated in SFEM II media supplemented with 50ng/ml of murine IL6 ...
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bioRxiv - Bioengineering 2022Quote: ... Mononuclear cells (MNCs) were separated from fresh CB samples by Lymphoprep™ density gradient medium (STEMCELL Technologies Inc.). Frozen CB samples were thawed and treated with 5,000 U/ml of DNase I (Worthington) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were plated onto 25 mm MatTek dishes and imaged in phenol-red free mTeSR1 (Stemcell Technologies, 85850). Cells were kept under 5% CO2 and 37 degrees C ...
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bioRxiv - Developmental Biology 2022Quote: ... stem cell media is replaced with ameloblast base media consisted of either EpiCult-C media (StemCell Technologies, #05630) or RPMI 1640 Medium (Thermo ...
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bioRxiv - Immunology 2022Quote: ... Gr1+ cells from MC38 tumors were isolated using the CD11b+ Gr1+ magnetic isolation kit (STEMCELL Technologies cat #19867) and incubated with NK cells isolated using the mouse NK cell isolation kit (STEMCELL Technologies cat #19855 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured into 96-well low-attachment plates containing NeuroCult Basal Media (Mouse and Rat; STEMCELL Technologies) with Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... both apical and basolateral media were removed and ALI differentiation media (Stem Cell Technologies, Pneumacult ALI Basal Medium) supplemented with 1X ALI Maintenance Supplement (StemCell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... 5 min) and plated at a density of 90,000 cells/mL in STEMdiff Neural Induction Medium (StemCell Technologies) with SMADi supplement (StemCell Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were passaged in small aggregates (1:7/1:10 depending on the line) using ReLeSR (StemCell Technologies). 10 μM Y-27632 (ROCK inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
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bioRxiv - Cancer Biology 2022Quote: ALDH activity was determined for MDA-MB-231 and MCF10A cells using the Aldefluor™ Assay (STEMCELL Technologies) according to manufacturer instructions with incubation conducted for 30min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... with daily media change and passaged at 80% confluence using Gentle Cell Dissociation Reagent (StemCell Technologies; Cat#07174). Cultures were routinely tested for mycoplasma using the MycoAlert Mycoplasma Detection kit (Lonza ...
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bioRxiv - Neuroscience 2024Quote: ... the generated CLN6- and CTL-I3N-IPSCs were dissociated into single cells using Accutase (Stemcell technologies, Cambridge, MA) and resuspended in DOX-induction medium (DMEM-F12 (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... mature microglial cells were isolated from astrocytes using EasySep Mouse CD11b Positive Selection Kit II (Stemcell Technologies #18970A,) and subsequently seeded into p60 plates ...
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bioRxiv - Neuroscience 2024Quote: FA000010 (RUCDR/BiologyX) iPSCs were differentiated into hematopoietic precursors cells (HPCs) using the STEMdiff Hematopoietic kit (STEMCELL Technologies) largely by manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... 500 and 1500 CD34+ cells were added to 1 mL of MethoCult™ H4034 Optimum medium (StemCell Technologies) and placed into one well of a 6-well plate ...
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bioRxiv - Neuroscience 2023Quote: ... hematopoietic lineage to obtain hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies, Catalog No. 05310). Next ...
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bioRxiv - Molecular Biology 2024Quote: All the hiPSC lines used in this study were cultured with mTeSRTM Plus medium (#05825, Stem Cell Technologies) in 6-well plates previously coated with MatrigelTM (#354230 ...
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bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Single-cell suspensions were then obtained from tissue organoids by serially dissociating with trypsin-EDTA (0.25%, STEMCELL Technologies), 5 mg/mL dispase (STEMCELL Technologies) ...
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bioRxiv - Immunology 2024Quote: ... cells were rapidly thawed at 37 °C in a water bath and added dropwise into IMDM (STEMCELL Technologies) containing 10% FBS and 10 μg/mL DNase (MilliporeSigma) ...
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bioRxiv - Immunology 2024Quote: ... The remaining cellular pellet volume was measured and the red blood cells lysed with ammonium chloride (STEMCELL Technologies). Cells were then stained to identify viability dyenegative CD45+ (BUV395 ...
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bioRxiv - Immunology 2024Quote: ... Cells were maintained in 1X Basal Media containing PneumaCult-Ex Plus Basal Medium (490mL) (STEMCELL Technologies, Vancouver, Canada), PneumaCult-Ex Plus 50X Supplement (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... 3D organoids were harvested from the basement membrane matrix using Gentle Cell Dissociation Reagent (100-0485, StemCell Technologies), fixed with 4% paraformaldehyde ...
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bioRxiv - Biophysics 2023Quote: Neural stem cells were differentiated from hiPSCs using STEMdiff™ Neural Induction Medium (Cat. No. 05839, STEMCell Technologies) monolayer culture protocol as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Culture EBs in an ultra-low-adherence plate with Stemdiff A (STEMdiff™ Hematopoietic Kit, Stem cell technologies) for 3 days and Stemdiff B (STEMdiff™ Hematopoietic Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... MLL-AF10 patient-derived xenograft (PDX) cells were cultured in IMDM medium supplemented with 20% BIT9500 (STEMCELL Technologies), human cytokines ...
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bioRxiv - Molecular Biology 2022Quote: ... Peripheral Blood Mononuclear Cells (PBMCs) were isolated from whole blood by Ficol centrifugation using SepMate tubes (STEMCELL Technologies) and subsequent isolation of CD3+ T cells using negative selection isolation kits (STEMCELL Technologies ...
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bioRxiv - Genetics 2023Quote: iPSCs were differentiated into neural progenitor cells (NPCs) using the STEMdiff SMADi Neural Induction Kit (STEMCELL Technologies; #08581) Monolayer Culture Protocol following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... For Il-2ra time course naïve CD4+ T cells were enriched from splenocytes using EasySep kit (StemCell Technologies). Approximately 100,000 cells were activated using 2ug/ml plate bound anti-CD3/CD28 ...
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bioRxiv - Immunology 2023Quote: ... or PEP-619WW C57BL/6 mice using a negative isolation kit (Stem Cell Technologies, Vancouver, British Columbia, Canada). A low number of isolated CD4 T cells ...
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bioRxiv - Genetics 2023Quote: ... the cells were grown using the daily medium changes of STEMdiff Forebrain Neuron Differentiation kit (STEMCELL Technologies, #08600) and then transitioned to the STEMdiff Forebrain Neuron Maturation kit (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2023Quote: Enriched CD34+ cells were resuspended to a final volume of 1.5 mL with semisolid Methocult (StemCell Technologies, #04404) and then plated in 6-cm dishes and incubated at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies, Vancouver, BC, Canada). Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies, Vancouver, BC, Canada). Floating HPCs were collected and plated at 50,000 cells/mL in STEMdiff microglia differentiation media (StemCell Technologies ...
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bioRxiv - Microbiology 2023Quote: ... both apical and basolateral media were removed and ALI differentiation media (Stem Cell Technologies, Pneumacult ALI Basal Medium) supplemented with 1X ALI Maintenance Supplement (StemCell Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... starting from freshly isolated PBMCs from 69 PLWH using the EasySep CD4 T cell isolation kit (Stemcell Technologies). CD4+ T cells were frozen as dry pellet at −80°C prior to DNA extraction with the DNeasy Blood & Tissue kit (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... Differentiation media was prepared by mixing equal volumes of IntestiCult™ (INT) Organoid Basal Medium (Stem Cell Technologies) and complete media without growth factors (CMGF- ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting sample was resuspended to a concentration of 50 million cells/mL in EasySep Buffer (STEMCELL Technologies). 50 μL/mL isolation cocktail and 50 μL/mL cocktail enhancer were added to the sample ...
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bioRxiv - Cancer Biology 2023Quote: ... “Monolayer Culture Protocol” from “Generation and Culture of Neural Progenitor Cells Using the STEMdiff Neural System” (STEMCELL Technologies) was used to generate third passage NPC-like cells ...
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bioRxiv - Immunology 2023Quote: Cells were cultured in Iscove’s Modified Eagle Medium (IMDM) supplemented with 20% serum substitute (BIT 9500; Stemcell Technologies), 1 µg/ml low density lipoprotein (Stemcell Technologies) ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated from patient blood by centrifugation with Lymphoprep (StemCell Technologies, Vancouver, Canada) and SepMate devices (StemCell Technologies ...
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bioRxiv - Developmental Biology 2023Quote: ... Aggrewell™400 was prepared by rinsing the wells with an anti-adherence rinsing solution (Stem cell technologies) as per the manufacturer’s protocol and washed with SL (without LIF ...
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bioRxiv - Neuroscience 2023Quote: ... the EBs were transferred to a 24-well ultra-low attachment plate containing Induction Medium (Stem Cell Technologies), where each well receives 1-2 EBs ...
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bioRxiv - Cell Biology 2023Quote: ... organoids were removed from matrix by incubation with ice-cold Gentle Cell Dissociation Reagent (STEMCELL Technologies, 100-0485) with gentle rocking at 4°C for 60 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Isolated progenitor cells were cultured for 48 hours in erythroid differentiation medium (Iscove Modified Dulbecco Medium, Stemcell Technologies) supplied with 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: iPSC lines were cultured on 60-mm rhVTN coated plates in StemFlex media (Stem Cell Technologies, cat. A3349401) and passaged using CTS EDTA Versene Solution (Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... the remaining fresh murine BM cells were flow sorted and subsequently cryopreserved in CryoStor® CS5 (STEMCELL Technologies). For secondary transplant ...