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Citations for Macherey-Nagel GmbH :
1651 - 1700 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Genomic DNA from thirty-three clones and WT tachyzoites extracted using NucleoSpin DNA RapidLyse kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... gDNA was extracted from larvae using NucleoSpin DNA Insect (Macherey-Nagel, Düren, Germany) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Selected clones were isolated using Nucleo Spin Plasmid kit (Macherey-Nagel) and verified by sequencing (SEQme ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were purified by the NucleoSpin Gel and PCR cleanup kit according to manufacturer’s instructions (Macherey-Nagel, Dueren, Germany). Purified products were sent for Sanger sequencing with primers rbcL404+ and rbcL587-to Macrogen (http://dna.macrogen-europe.com).
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bioRxiv - Microbiology 2022Quote: ... The products were inspected on agarose gel and purified using Nucleofast PCR purification plates (Macherey-Nagel, Dueren, Germa) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted using NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel, Cat #740955.250) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from cell pellets using the NucleoSpin RNA II Mini kit (Macherey-Nagel), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells and supernatant) using either the NucleoSpin RNA II Mini kit (Macherey-Nagel) or the RNeasy Mini kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... and then purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel). Amplicons were then assembled (1:1 insert:vector molar ratio ...
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bioRxiv - Microbiology 2022Quote: ... DNA was extracted using the NucleoMag® VET kit (Macherey-Nagel) and the KingFisher® extraction platform following manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... Digested DNA was column purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). 2 μg of purified DNA was ligated to adapters containing specific barcode sequences ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 mM NaCl and 1 mg/ml proteinase K (Macherey-Nagel, 740506) and 0.05 mg/ml RNase A (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... De-crosslinking was performed by adding 20 µl Proteinase K (20 mg/ml, Macherey-Nagel, 740506) to the ligation mix (1.2 ml ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplified fragments were purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany) and DNA concentrations were measured by NanoDrop.
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bioRxiv - Microbiology 2022Quote: ... Low copy plasmids were extracted by NucleoBond Xtra Midi kit (MACHEREY-NAGEL) with the other plasmids were extracted by plasmids midi kit (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... nidulans mycelia after 9 h incubation using a NucleoSpin RNA Plant kit (Macherey-Nagel) and RNase-Free DNase (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... RNA was isolated from peripheral blood mononuclear cells (PBMC; NucleoSpin RNA Mini Plus, Macherey-Nagel GmbH & Co.KG, Düren, Germany). For cDNA first strand synthesis RevertAid kit (Thermo Scientific ...
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bioRxiv - Zoology 2022Quote: Total genomic DNA was extracted using the NucleoSpin Tissue Kit (Macherey-Nagel, Germany) following the standard protocol for animal tissue ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA for cell-free reactions was prepared from overnight bacterial cultures using Maxiprep kits (Macherey-Nagel).
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 250 μg/mL of RNase A (Macherey-Nagel) and 0.5 mg/mL of lysing enzymes from Trichoderma harzianum (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: ... Mini kit for RNA purification with DNA removal column (Macherey-Nagel, cat# 740984.50). cDNA synthesis was performed using EvoScript Universal cDNA Master (Roche ...
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bioRxiv - Microbiology 2022Quote: Large-scale purification of BAC/YAC plasmid DNA was done using a NucleoBond® Xtra BAC (Macherey-Nagel) kit ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was transferred to a fresh falcon and directly used for immobilized metal affinity chromatography (IMAC) using the Äktapure25 system with a sample pump (Cytiva) and 1ml Ni-NTA columns (Macherey-Nagel). Proteins were concentrated where necessary using Amicon filter devices with appropriate molecular weight cut-off (Millipore ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from the rotten food waste with NucleoSpin DNA Stool kits (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and quality checked by using a NanoDrop (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2022Quote: ... a bulk (∼ 300,000 Euplotes cells) extract with the NucleoSpin™ Tissue Kit (Macherey-Nagel™) was processed through a SQK-LSK109 ligation-sequencing library ...
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bioRxiv - Microbiology 2022Quote: ... Intracellular and supernatant HBV DNAs were isolated using the Nucleospin Tissue extraction kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Table S1) and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). 3 to 4×107 cells were transfected with 3 to 8 μg of DNA in 100 μl bloodstream form transfection buffer (Schumann Burkard ...
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bioRxiv - Microbiology 2022Quote: ... BAC DNA was purified using the NucleoBond BAC 100 kit (Macherey-Nagel #740579) and were transfected into early passage NIH-3T3 cells in 6 well plates using TransIT-X2® dynamic delivery transfection system (Mirus) ...
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bioRxiv - Microbiology 2022Quote: ... The total genomic DNA of the insect individual and its symbionts was extracted using the NucleoSpin Tissue XS Kit (MACHEREY-NAGEL, Duren, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Cells were harvested and total RNA was isolated using either the Nucleospin RNA mini kit (MACHEREY-NAGEL) or the Quick-DNA/RNA™ Viral kit (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: Total DNA was isolated from frozen cell pellets using the NucleoSpin Microbial DNA Kit (Macherey-Nagel, Düren, Germany). Methods for DNA quality control and quantification were reported previously [28] ...
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bioRxiv - Microbiology 2022Quote: ... or the NucleoSpin Tissue kit (Macherey-Nagel #740952-250) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... For Clr purification the initial purification was conducted using a 5 mL Protino™ Ni-NTA Column (Macherey-Nagel). Size-exclusion chromatography over a 16/600 Superdex 200 column (GE ...
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bioRxiv - Microbiology 2022Quote: ... The clarified lysate was subjected to affinity chromatography on Ni2+ agarose resin (Macherey-Nagel) and washed extensively with lysis buffer ...
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bioRxiv - Microbiology 2022Quote: Viral RNA from the supernatant was extracted using the NucleoSpin RNA Virus Isolation Kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted with a ‘NucleoSpin RNA Mini’ RNA purification kit (Macherey-Nagel). Samples were purified twice with an extra DNase digestion step between purifications ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from ACE-grown cells (DSMZ medium 461 with 5 mM ACE) with the NucleoSpin® Microbial DNA kit (Macherey-Nagel, Düren, Germany) according to the manual and using wide-bore tips ...
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bioRxiv - Microbiology 2022Quote: DNA was extracted from isolates using a NucleoSpin Microbial DNA kit (Macherey-Nagel, Duren, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ACE and its metabolites ANSA and acetoacetate were quantified by HPLC with an UV/VIS detector employing a Nucleosil 100-5 C18 HD column (240/3 mm, Macherey-Nagel) and an eluent at 0.5 ml min−1 containing 100 mM NaH2PO4 and 10 mM tetrabutylammoniumhydrogensulfate ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA (gDNA) was extracted using a NucleoSpin Microbial DNA kit (Macherey-Nagel, PA, USA) and TissueLyser II (Qiagen ...
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bioRxiv - Microbiology 2022Quote: ... One hundred mg of soil were used as starting material using the NucleoSpin Soil DNA kit (Macherey-Nagel, Germany). The whole procedure was carried out under laminar flow ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA from the LAB strains was extracted using a NucleoBond AXG column and Buffer Set III (U0544A & U0603A, MACHEREY-NAGEL, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... followed by the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel), and sent to Eurofins (Ebersberg ...
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bioRxiv - Microbiology 2022Quote: Swab tips were excised using sterile scissors and were subject to DNA extraction using the NucleoSpin Soil DNA extraction kit (Macherey-Nagel Inc., Allentown, PA; catalogue: 740780) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... gDNA was purified using the NucleoSpin gDNA clean-up kit (Macherey-Nagel) and stored at −20°C.
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bioRxiv - Molecular Biology 2022Quote: ... and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel). The selection markers for fungal transformation were cloned into digested plasmids by using the Rapid DNA Ligation Kit (Thermo) ...
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bioRxiv - Microbiology 2022Quote: ... coli using the Nucleospin RNA Extraction Kit (Macherey-Nagel) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: Total DNA was isolated from cells using NucleoSpin Tissue Kit 192 (Macherey-Nagel) and the KSHV genome copies were quantified by qPCR using primers specific for LANA ...
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bioRxiv - Molecular Biology 2022Quote: ... NucleoSpin Gel and PCR Clean-up kit was purchased from Macherey-Nagel. 2 mm electroporation cuvettes were acquired from Cell Projects Ltd ...