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Citations for Macherey-Nagel GmbH :
1451 - 1500 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated using the Nucleospin RNA plus kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from HeLa cells using the NucleoSpin RNA Kit (Macherey-Nagel). Samples were prepared in duplicates ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel). 200 ng of the extracted RNA was reverse-transcribed to cDNA using the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Synthetic Biology 2022Quote: ... Colonies were sequence confirmed and then maxi-prepped per the manufacturer’s recommendations (NucleoBond Xtra Maxi; Macherey-Nagel). Plasmids were sterile filtered using a 0.22-μm syringe filter and stored at 4°C.
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bioRxiv - Microbiology 2022Quote: ... 4°C) and 100 µL of the supernatant was collected in gaschromatography (GC) glass vials (Macherey-Nagel, Düren, Germany); both were stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial DNA was extracted from mice stool using NucleoSpin DNA Isolation kit (740472.50 Macherey-Nagel, Germany) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Bacterial DNA was extracted from mice stool using NucleoSpin DNA Isolation kit (740472.50 Macherey-Nagel, Germany) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from infected and non-infected HCT8 cells using NucleoSpin RNA Kit (Macherey-Nagel, Germany). An on-column DNase digestion with a RNase-free DNase was included in the process described by the fabricant to remove any genomic DNA contamination in RNA samples ...
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bioRxiv - Molecular Biology 2022Quote: RNA from cell culture supernatant after CPZ/PCZ treatment (mentioned above) was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative RT-PCR was performed in Roche LightCycler 480 using commercial kits ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA from inflorescences was extracted with the NucleoSpin RNA Plant kit (Macherey-Nagel) following the manusfacturer’s protocol and adding an on-column DNase treatment ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial RNA was extracted using NucleoSpin RNA Isolation kit (Macherey-Nagel, Germany) following manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: Total RNA was extracted from kidney tissues using the Nucleospin RNA II kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... synaptosomes and tissues were homogenized using a dounce tissue grinder in NucleoZOL (Macherey-Nagel 740404) supplemented with 5 mM of DTT (Thermo Scientific R0862 ...
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bioRxiv - Cell Biology 2022Quote: ... total RNAs were extracted using a column-based kit (Cat. 740955.250, Macherey-Nagel). For cells ...
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bioRxiv - Genetics 2022Quote: ... cells were scraped from plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel). The resulting plasmid DNA was used for yeast transformation.
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bioRxiv - Genetics 2022Quote: ... The protein concentration was determined by Protein Quantification Assay (Cat.# 740967,Macherey-Nagel). 10 µg of the protein was separated by SDS-PAGE electrophoresis ...
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bioRxiv - Genetics 2022Quote: ... the cells were scraped from the plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Genetics 2022Quote: ... PCR products were isolated by gel extraction (Macherey-Nagel) and sequenced with a primer located upstream of the I-SceI cut site (DR-white2 ...
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bioRxiv - Genetics 2022Quote: ... Multiple PCR reactions for the 5s rDNA amplicon were pooled and column-purified (Macherey-Nagel). 1µg of DNA was labeled with digoxigenin (for chromosome III ...
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bioRxiv - Developmental Biology 2022Quote: gDNA from 6 male and 5 female pupae (sexed by eye) was extracted using the NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany), pooled by sex and used as template for subsequent PCRs ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was extracted (Nucleospin RNA kit, Macherey-Nagel, 740955). The remaining part of embryos were processed for whole-mount in situ hybridisation detection of Uncx4.1 and accurate somite count determination.
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bioRxiv - Developmental Biology 2022Quote: ... RNA was extracted using the Total RNA Isolation Kit (Macherey-Nagel, Germany) according to manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was extracted (Macherey-Nagel) and cDNA prepared (Roche ...
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bioRxiv - Microbiology 2022Quote: DNA from monolayer cultures as well as from rafts was isolated using the NucleoSpin Blood QuickPure kit (740569.250; Macherey-Nagel) according to the manufacturer’s instructions with some modifications for raft tissues ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from cells following the manufacturer’s instructions (Macherey-Nagel NucleoSpin RNA cat. 740956). Reverse transcription was performed using SuperScript III reverse transcriptase (Thermo Fisher cat ...
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bioRxiv - Microbiology 2022Quote: ... the cultures centrifuged (15 min at 2700×g) and filtered through a 0.2 μm membrane (Chromafil® Xtra PET 20/25, Macherey-Nagel, Germany) were analyzed using a Shimadzu high-performance liquid chromatograph (HPLC ...
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bioRxiv - Microbiology 2022Quote: ... and/or bovine pancreatic ribonuclease (Roche or Macherey-Nagel (MN)) ...
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bioRxiv - Molecular Biology 2022Quote: ... single colonies were picked and transferred into bacterial starter cultures and incubated for ∼6 h for mini-prep (Macherey-Nagel, NucleoSpin Plasimd Mini kit for plasmid DNA ...
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bioRxiv - Molecular Biology 2022Quote: ... or maxi-prep (Macherey-Nagel, NucleoBond Xtra Maxi Plus, 740416.50) DNA purification according to the standard manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... The digested backbone was purified with NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, 740609.50), aliquoted ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from around 5×105 to 1×106 cells using the NucleoSpin RNA Plus (MACHEREY-NAGEL, 740984.50) or Quick-RNA Miniprep Kit (Zymo Research ...
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bioRxiv - Physiology 2022Quote: ... Japan) equipped with an analytical CN-RP column (NUCLEODUR 100-5, CN-RP, 150 × 4.6, 5 µm column; Macherey-Nagel GmbH & Co. KG, Düren, Germany) and a UV-diode-array detector ...
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bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Nucleozol (Macherey-Nagel, Hoerdt, France) was added on top of the cell layer/bone powder and total RNA were purified with Nucleospin RNA set nucleozol column (Macherey-Nagel ...
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bioRxiv - Systems Biology 2022Quote: ... Genomic DNA from the cell pellets was purified using the NucleoSpin blood L kits (Macherey-Nagel) according to the manufacturer’s instructions and was quantified using PicoGreen dsDNA assay kits (ThermoFisher).
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bioRxiv - Immunology 2022Quote: ... The protein was purified on a Ni-NTA resin column (Macherey-Nagel) and the eluted fractions were concentrated and dialyzed against PBS using the same Amicon system as described above ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The obtained PCR products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel GmbH, Düren, Germany) according to supplier’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the cells were collected from the plate and plasmid pools were extracted using the NucleoBond Xtra Midi Kit (Macherey-Nagel) according to the manufacturer’s recommendation.
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bioRxiv - Evolutionary Biology 2022Quote: ... 35 mg of animals were transferred into a 1.5 mL tube and total RNA was extracted using a Nucleospin® RNA Plus Kit (Macherey-Nagel, Düren, Germany). The prey cDNA library was screened for interactions with the 70 distal C-terminal amino acids of the TadNaC2 and TadNaC10 channels expressed in yeast from the bait vector pGBK-T7 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products with multiple bands were excised and purified from gel using the Nucleospin Gel and PCR Clean-up kit from Macherey-Nagel, or cloned using the NEB® PCR cloning kit (New Englands BioLabs ...
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bioRxiv - Genetics 2022Quote: RNA extraction was performed from frozen tissue in culture plates (P6 well plate, Falcon®) by using the NucleoSpin RNA kit (Macherey-Nagel). RNA concentration was measured with NanoDrop1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Genetics 2022Quote: ... according to the manufacturer’s instructions (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). Standard PCR reactions were performed using KAPATaq DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... the Macherey-Nagel NucleoMag Food kit (Cat# 744945.1, Macherey-Nagel, Düren, Germany), and the ZymoBIOMICS 96 MagBead DNA Kit (Cat# D4302 ...
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bioRxiv - Genetics 2022Quote: DNA was extracted from frozen cells pellets with the Nucleospin tissue DNA extraction kit (Macherey-Nagel). DNA concentration was evaluated using the NanoDrop1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... BEAS-2B and MRC-5 using NucleoSpin® Viral RNA Isolation kit (Macherey-Nagel). One-Step RT-PCR SuperScript™ III Platinum™ Kit (Life Technologies ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PV-B was isolated with the NucleoBond Xtra Midi Plus kit (MACHEREY-NAGEL, USA), and introduced into bacteria by electroporation as in 37 ...
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bioRxiv - Immunology 2022Quote: ... and purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel).
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bioRxiv - Cancer Biology 2022Quote: ... Plasmid DNA was purified using NucleoBond® Xtra Maxi EF (Macherey-Nagel, Cat. No. 740424) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and the Macherey-Nagel Mini kit Nucleospin protocol (Macherey-Nagel, #740955.50).