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Citations for Macherey-Nagel GmbH :
1451 - 1500 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from only the 13C-acetate grown cultures that yielded enough DNA at 94 days of incubation using the NucleoSpin Microbial DNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the cellular RNA was extracted by NucleoSpin RNA Kit (Macherey-Nagel GmbH&Co. KG, Germany) for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Low copy plasmids were extracted by NucleoBond Xtra Midi kit (MACHEREY-NAGEL) with the other plasmids were extracted by plasmids midi kit (QIAGEN ...
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bioRxiv - Plant Biology 2023Quote: PCR products were pooled by 6 to 12 according to the linkers and cleaned using the Nucleospin Gel and PCR clean up (Macherey-Nagel). Samples containing asymptomatic plants were pooled separately from those containing symptomatic leaves to limit potential cross-contaminations of highly concentrated viruses in symptomatic pools ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was isolated was using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2023Quote: ... approximately 50 mg of ground tissue were extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany) following the user guidelines ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from the roots of 21-days old mock- and SA190-colonized Arabidopsis grown on ½ MS with or without 25% PEG with the Nucleospin RNA plant kit (Macherey-Nagel) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... purified using the NucleoSpin plasmid kit (Macherey-Nagel, Düren, Germany), were confirmed by sequencing.
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bioRxiv - Microbiology 2023Quote: DNA was extracted for Termignon cheese isolates and all new strains sequenced using the Illumina technology for this project from fresh haploid mycelium and conidia after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel). Sequencing was performed using the Illumina paired-end technology (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... and NucleoBond® Xtra Midi Plus (MACHEREY-NAGEL, Düren, Germany) for small and large scales ...
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bioRxiv - Microbiology 2023Quote: ... Replicon RNA was extracted using NucleoSpin®RNA (MACHEREY-NAGEL) at the indicated time points and its copy number was measured using the THUNDERBIRD Probe One-step qRT-PCR Kit (TOYOBO CO. ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted following standard protocols using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany). DNA was quantified using a Qubit 3 fluorometer in combination with dsDNA BR or HS assay kits (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
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bioRxiv - Microbiology 2023Quote: ... PCR-column purified (Macherey-Nagel), and sent for Sanger sequencing at Genewiz with the primers in Table 2.
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bioRxiv - Microbiology 2023Quote: ... 50 μl of a 40% slurry Ni-NTA agarose (20 μl beads, Macherey-Nagel) were added followed by an incubation for 45 min at 4°C with shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... and rDNase (Macherey-Nagel) and reverse-transcribed with ReverTra Ace® (TOYOBO ...
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bioRxiv - Molecular Biology 2023Quote: ... The ligated products were transformed into XL1 blue bacterial cells by electroporation followed by isolating the plasmids from single bacterial colonies using NucleoBond Xtra Midi kit (Macherey-Nagel). The Cas9-sgRNA plasmids were validated by BbsI restriction analysis and confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... kit with NTB binding buffer (#740595.150, Macherey-Nagel) according to the manufacturer’s instructions and eluted in 30 µL.
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bioRxiv - Pathology 2023Quote: ... we isolated mtDNA from postnuclear fractions with the alkaline denaturation-neutralization procedure (Nucleospin Plasmid Mini kit, Macherey-Nagel). Liver tissue was homogenized and nuclei pelleted (1000g 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... Total DNA was extracted using CTAB (Doyle & Doyle 1987) and purified using NucleoSpin® Purification Kit (Macherey-Nagel, Düren, Germany). DNA extractions were profiled in Agarose gel 1% and using Nanodrop ...
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bioRxiv - Zoology 2023Quote: ... Germany) or on a hydrophilic interaction liquid chromatography (HILIC) Nucleodur column (125 × 3 mm, 3 μm, Macherey-Nagel, Düren, Germany) for normalphase chromatography ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from Arabidopsis seedling tissues with NucleoZOL Reagent (Macherey-Nagel) according to the manufacturer’s protocol and gene expression analysis was carried out as previously described (Locci et al. ...
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bioRxiv - Microbiology 2023Quote: RNA was isolated using the NucleoSpin RNA kit from Macherey-Nagel according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The BoNT-X/NTNH complex was purified by gravity-flow affinity chromatography on Ni-NTA agarose resin (Macherey-Nagel, Düren, Germany) in lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... Mini kit for plasmid DNA (Macherey-Nagel). Correct mutation of the plasmids was verified by sequencing performed by MicroSynth GmbH Göttingen.
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bioRxiv - Biochemistry 2023Quote: ... and reverse transcription were performed using NucleoZOL (Macherey-Nagel), Amplification Grade DNase I (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Nucleozol (Macherey-Nagel, Hoerdt, France) was added on top of the bone powder and total RNA were purified with Nucleospin RNA set nucleozol column (Macherey-Nagel ...
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bioRxiv - Biochemistry 2023Quote: ... The remainder of the acidic hydrolysate was cleaned on an SPE column (SPE Chromabond crosslinks, Macherey-Nagel). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... was added on top of the bone powder and total RNA were purified with Nucleospin RNA set nucleozol column (Macherey-Nagel) according to the manufacturer recommendations ...
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bioRxiv - Biochemistry 2023Quote: ... SUV420H1 proteins were isolated by incubating the soluble fraction for 2 hrs with GST beads (Macherey-Nagel). The beads were washed with two column volumes of wash buffer (20 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... PCR products were purified using a PCR clean-up kit as per manufacturer’s instructions (Macherey-Nagel, Germany) followed by agarose (1 w/v % ...
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bioRxiv - Biochemistry 2023Quote: ... of a saline solution (0.9%) and immediately stored at −20°C in 10 vol NucleoProtect RNA stabilization reagent (Macherey-Nagel) before further RNA extraction and target mRNA quantification.
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was incubated with preequilibrated Protino Ni-NTA beads (1 ml resin, Macherey-Nagel) at 4°C for 1h ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant discarded and the beads transferred onto a Protino 14 ml gravity column (Macherey-Nagel). After 20 ml of additional equilibration using buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... RNA was extracted using Nucleospin RNA kits (#740955.250, Macherey-Nagel). Complementary DNA was synthetized using iScript RT Supermix kit (BioRad) ...
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bioRxiv - Microbiology 2023Quote: Desalting of cleared peptide samples was performed in C18 solid phase extraction (SPE) cartridges (Macherey-Nagel). First ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted from 1.106 GSCs using the NucleoSpin RNA Plus purification kit (Macherey-nagel, 740955). Equal amounts of RNA were reverse-transcribed using the Maxima First Strand cDNA Synthesis kit (Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and NucleoSpin RNA (Macherey-Nagel).
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bioRxiv - Evolutionary Biology 2023Quote: ... genomic DNAs were extracted from muscle using NucleoSpin Tissue (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Messenger RNAs (mRNAs) were prepared using a Nucleospin RNA Kit (Macherey-Nagel), and complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were evaluated by electrophoresis and then further processed with the PCR clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was isolated from cells using the NucleoSpin® RNA Kit (MACHEREY-NAGEL, Germany), and reverse transcription was performed using the qScript cDNA Synthesis Kit (Quantbio ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 1x with PBS and lysed in 350 μl of Guanidine thiocyanate-based lysis buffer (LBP lysis buffer, Macherey-Nagel NucleoSpin RNA plus isolation kit ...
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bioRxiv - Cell Biology 2023Quote: BAC DNA for transfection was prepared using the BAC100 Nucleobond kit (Macherey-Nagel, Germany). Cells were transfected using ExGen500 transfection reagent (Fermentas ...
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bioRxiv - Cell Biology 2023Quote: ... Isolation of RNA was performed using the Nucleospin® RNA isolation Kit (Macherey-Nagel). The samples were stored at -80°C until they were sent for transcriptomic analysis.
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bioRxiv - Cancer Biology 2023Quote: ... Gel bands were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, N. 740609.50) and sequenced by Sanger sequencing.
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bioRxiv - Cell Biology 2023Quote: Total RNA extraction and complementary DNA (cDNA) synthesis were performed using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany) and High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR clean-up was performed on the correct RT-PCR products according to the manufacturer’s instructions (Macherey-Nagel, #740609.250). 4-6 ng of each product were sent for Sanger sequencing to Eurofins Genomics Germany GmbH.
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from TGF-β1-treated cells (as described above) using NucleoSpin® RNA kit (Macherey-Nagel) according to manufacturer’s instructions.