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Citations for Macherey-Nagel GmbH :
1751 - 1800 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: mRNA was isolated using NucleoSpin RNA XS kit (Macherey-Nagel, 740902.50) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was purified with NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany), and sequenced by Eurofins Genomics ...
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bioRxiv - Microbiology 2022Quote: ... The nucleoSpin plasmid easypure Kit (Macherey-Nagel) was used for plasmid purification and expression cassettes were sequence verified ...
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bioRxiv - Microbiology 2022Quote: ... caecal and colonic tissue was isolated by using the NucleoSpin RNAII kit (Macherey-Nagel GmbH) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... or NucleoSpin® RNA Virus (Macherey-Nagel GmbH, Düren, Germany). BHK-21 cells were infected with VSV-G-3CLpro-L wild type and F305L (3CLpro ...
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bioRxiv - Microbiology 2022Quote: ... The 0.22 µm membrane was used for bacterial DNA isolation using NucleoMag DNA/RNA Water Kit (MACHEREY-NAGEL Inc., Bethlehem, PA) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fragments were gel purified with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel). The 3×FLAG-tag (DYKDHDGDYKDHDIDYKDDDDK ...
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...
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bioRxiv - Molecular Biology 2022Quote: ... at least eight separate tubes were used for PCR and then pooled and purified by using PCR and gel extraction kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: TRIM37 amplicons containing insertions were purified using gel extraction kit (Macherey-Nagel) after 1.5 % agarose gel gel-electrophoresis ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... total RNA after McIdas RNAi was isolated from U2OS cells using the Nucleospin RNA II kit (Macherey-Nagel). 1 μg RNA was converted to cDNA using M-MLV transcriptase (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR fragments were further subjected to PCR clean-up using PCR clean-up kit (Macherey-Nagel™). Electrocompetent cells were produced according to the Invitrogen Pichia Expression Kit (Catalogue no ...
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bioRxiv - Molecular Biology 2022Quote: ... All PCR fragments for cloning and plasmids were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and GenElute™ Plasmid Miniprep Kit (Merck) ...
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bioRxiv - Molecular Biology 2022Quote: ... further plasmid DNAs of each clone were prepared by using Nucleospin Plasmid (Macherey-Nagel) and sent for Sanger sequencing (Azenta ...
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bioRxiv - Microbiology 2022Quote: ... DNA was purified by using the gDNA clean-up kit (Macherey-Nagel). DNA concentrations and purity were checked using NanoDrop® (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted using the NucleoSpin RNA Plant kit (Macherey-Nagel). RNA samples (1µg each ...
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bioRxiv - Molecular Biology 2022Quote: Total DNA from cultured cells was isolated using a NucleoSpin Tissue Purification Kit (Macherey-Nagel; 740952) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids were isolated from 1 mL overnight culture using NucleoSpin Plasmid Easypure columns (Macherey-Nagel) from the Plasmid Easypure Kit (Nucleospin).
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bioRxiv - Microbiology 2022Quote: ... or stored in -80C for DNA extraction performed by NucleoSpin Tissue Kit (Macherey-Nagel) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... ~500 mg of rhizosphere soil was used for the NucleoSpin Soil gDNA Extraction Kit (Macherey-Nagel, Germany), and ~130 mg of roots for the DNeasy Plant DNA Extraction Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... was digested with BsmBI and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Forward and reverse oligos containing the guide sequence of interest were phosphorylated and annealed and ligated into the parental plasmid with T4 PNK and T4 DNA ligase ...
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bioRxiv - Molecular Biology 2022Quote: Cellular RNA for cDNA synthesis was purified with the NucleoSpin RNA Kit (MACHEREY-NAGEL) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was purified with the NucleoSpin RNA Kit (MACHEREY-NAGEL) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... The BAG6 target region was amplified from the genomic DNA from all samples using Q5 High-Fidelity Master Mix and subsequently purified using a NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. The purified samples were sent for sanger sequencing and the proportion of BAG6 knockout cells in each sample was estimated using the ICE CRISPR analysis tool (https://www.synthego.com/products/bioinformatics/crispr-analysis).
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bioRxiv - Molecular Biology 2022Quote: RNA from mESC and HEK293T cells was extracted with NucleoZOL (Macherey-Nagel); from MCF7 cells BIO TRI RNA reagent (Bio-lab ...
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bioRxiv - Molecular Biology 2022Quote: ... the NucleoSpin Miniprep kit was used (Macherey-Nagel 740490), and plasmids were eluted in 50-60 µL elution buffer ...
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bioRxiv - Cell Biology 2022Quote: Cells were lysed in 350 µl of RA1 lysis buffer (Macherey-Nagel, Düren, Germany) supplemented with 1% β-mercaptoethanol and stored at -80 °C (maximum one month ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated using a NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from SuBK12-08 cells using NucleoSpin RNA plus (MACHEREY-NAGEL) according to the manual ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted RNA was subsequently enriched for polyadenylated mRNA transcripts using the NucleoTrap mRNA enrichment kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: RNA from viral supernatants was isolated using Nucleospin Viral RNA isolation kit (Macherey-Nagel GmbH & Co. KG). qRT-PCR was carried out using nCOV-19 RT-PCR detection kit from Q-line Molecular to quantify SARS-CoV-2 RNA following manufacturer’s protocol on Roche LightCycler 480.
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bioRxiv - Cancer Biology 2022Quote: ... plasmid DNAs were recovered from ampicillin resistant colonies using a kit (NucleoBond Xtra Midi, Macherey-Nagel GmbH, Düren, Germany), and the nucleotide sequences of each construct confirmed as correct by Sanger direct cycle sequencing using a U6-specific sequencing primer.
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bioRxiv - Microbiology 2022Quote: The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from control and IMCD PCM1 KO cells before SAG treatment and 24 h after SAG treatment using NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and 1:8 of His-SUMO-CREB and MBP-Jacob-45-228) were incubated with GST-LMO4 and GST (control protein) immobilized on Protino Glutathione Agarose 4B beads (Macherey-Nagel). Probes were eluted with 25µl of 2x SDS sample buffer after incubation and washing steps (washing buffer ...
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bioRxiv - Genomics 2022Quote: ... The DNA band at 5 to 10kb size was collected for extraction with a NucleoSpin Gel and PCR Clean-up purification kit following the manufacturer’s instructions (Macherey-Nagel, cat# 740609.250, Germany), and was eluted with 15 μl NF water.
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bioRxiv - Plant Biology 2022Quote: ... flat bottom screw neck vials (MACHEREY-NAGEL GmbH & Co. KG, Germany Cat no. 702293), and sealed with an N 9 PP screw cap with a center hole and silicone white/PTFE red septum (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Microbiology 2022Quote: ... a NucleoSpin 96 Stool (Macherey-Nagel) kit was used to extract bacterial DNA from ∼200μL aliquots of the meconium samples ...
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bioRxiv - Synthetic Biology 2022Quote: ... We then collected spheroplasts by centrifugation and isolated RNA using the NucleoSpin RNA Plus kit (Macherey-Nagel). We evaluated RNA quality and integrity by Qubit fluorometry using an RNA BR Assay Kit (Thermo) ...
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bioRxiv - Zoology 2022Quote: ... osakensis queen was measured using pH paper (pH range 5.5–9.0; Macherey-Nagel). The pH of the haemolymph from a cut on the trochanter of the hind leg was also measured as a control.
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bioRxiv - Plant Biology 2022Quote: ... O23 and d44a genomic DNA was extracted from liquid-cultured aerial hyphae using the NucleoBond high-molecular-weight DNA kit (MACHEREY-NAGEL, Germany). The genomic DNA was processed through the short-read eliminator kit XL (Circulomics) ...
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bioRxiv - Plant Biology 2022Quote: ... we used the NucleoBond high-molecular-weight DNA kit (MACHEREY-NAGEL, Germany). After DNA extraction ...
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bioRxiv - Plant Biology 2022Quote: ... in pyridine followed by a 30 min treatment at 37 °C with 70 μl of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). An autosampler Gerstel Multi-Purpose system (Gerstel GmbH & Co.KG ...
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bioRxiv - Synthetic Biology 2022Quote: ... gDNA was extracted from 10 individuals from pools bgcn-Cas9D using the NucleoSpin Tissue kit (Macherey-Nagel). DNA was digested with the restriction enzymes BamHI ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cultures using a NucleoSpin Plasmid kit (Macherey-Nagel, Germany), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... gDNAs were extracted using the NucleoSpin Tissue kit (Macherey-Nagel). Approximately 500 bp surrounding the sgRNA target sites was amplified using primers listed in Table S11 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Genomic DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel). Further sequencing was carried out by Illumina MiSeq using primers LA4507 ...
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bioRxiv - Synthetic Biology 2022Quote: A NucleoSpin PCR clean-up gel extraction kit (Macherey-Nagel, Germany) was used to purify PCR products before their use in cloning ...
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bioRxiv - Synthetic Biology 2022Quote: ... Genomic DNA was extracted from each founder male using the NucleoSpin Tissue kit (Macherey-Nagel) and subjected to two separate PCR reactions with primers LA2750 ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from triplicate samples with the NucleoSpin™ RNA Plant Kit (Macherey-Nagel), and the RNA concentration and integrity (RIN ...