Labshake search
Citations for Macherey-Nagel GmbH :
1801 - 1850 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted from cells using the Nucleospin RNAII kit (Macherey-Nagel). One µg of total RNA was reverse-transcribed using the iScript cDNA Synthesis Kit (BioRad) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... was added on top of the cell layer/bone powder and total RNA were purified with Nucleospin RNA set nucleozol column (Macherey-Nagel) according to the manufacturer recommendations ...
-
bioRxiv - Plant Biology 2022Quote: ... The PCR products were checked out in an agarose gel and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Finally ...
-
bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
-
bioRxiv - Physiology 2022Quote: ... filtrated through 0.20 μm regenerated cellulose filters (Macherey-Nagel, Düren, Germany) and immediately analyzed by UHPLC without further preparation.
-
bioRxiv - Plant Biology 2022Quote: ... for 10 min at 37°C and then cleaned up with the NucleoSpin RNA clean-up kit (Macherey-Nagel). RNA quality was verified on a 2100 Bioanalyzer Instrument (Agilent ...
-
bioRxiv - Plant Biology 2022Quote: ... treated with a rDNase (Macherey-Nagel) for 10 min at 37°C and then cleaned up with the NucleoSpin RNA clean-up kit (Macherey-Nagel) ...
-
bioRxiv - Plant Biology 2022Quote: ... Total RNAs were extracted using the Nucleospin RNA Plus Kit (Macherey-Nagel, Düren, Germany). cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT ...
-
bioRxiv - Molecular Biology 2022Quote: ... Certain PCR products were purified from the gel using NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) and cloned individually in a TA-cloning vector called pGEM®-T Easy Vector (Promega ...
-
bioRxiv - Bioengineering 2022Quote: ... containing an insert (15 mm tip, wide opening, 0.2 mL, 6×31 mm, clear, Macherey-Nagel GmbH & Co. KG Germany) for High-Performance Liquid Chromatography (HPLC ...
-
bioRxiv - Microbiology 2022Quote: ... The product of barcoding qPCR was cleaned by gel extraction using NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL, GmbH & Co, KG, Germany), after 1% AGE electrophoresis run for 1 hour at 100 V ...
-
bioRxiv - Bioengineering 2022Quote: ... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
-
A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... RNA bands according to the size of the 18s and 28s rRNA were cut from the agarose gel and extraction was performed using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel). The extraction was performed according to the manufactureŕs protocol for RNA extractions from agarose gels using the buffer NTC (Macherey Nagel).
-
bioRxiv - Evolutionary Biology 2022Quote: ... These were obtained by using as template a 1:10 serial dilution (8 dilutions) of a cleaned (Macherey-Nagel’s NucleoSpin Gel and PCR Clean-up) and quantified (Invitrogen’s Qubit™ ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... using the NucleoSpin 96 Plant Kit (Macherey-Nagel, Düren, Germany; for buds and leaves) and the E.Z.N.A ...
-
bioRxiv - Cancer Biology 2022Quote: Total RNA was isolated from cells using the NucleoSpin® RNA Kit (MACHEREY-NAGEL, Germany), and reverse-transcription was performed using qScript cDNA Synthesis Kit (Quantbio ...
-
bioRxiv - Neuroscience 2022Quote: Total tissue RNA was isolated using the NucleoSpin RNA II Kit (Macherey-Nagel). cDNA synthesis was performed by reverse transcription using the Transcriptor High Fidelity cDNA-Synthesis Kit (Roche ...
-
bioRxiv - Synthetic Biology 2022Quote: ... SLS was precipitated with trifluoroacetic acid (TFA, 1.5% final concentration) and peptides were purified using Chromabond C18 microspin columns (Macherey-Nagel). Acidified peptides were loaded on spin columns equilibrated with 400 μL acetonitrile and then 400 μL 0.15% TFA ...
-
bioRxiv - Synthetic Biology 2022Quote: Genomic DNA was obtained using the NucleoBond HMW DNA kit (740160.20, Macherey-Nagel, Düren, Germany) according to the manufacturer guidelines using lyticase (#L4025 ...
-
bioRxiv - Microbiology 2022Quote: ... DNA extraction from the pellets was performed using the NucleoSpin 96 Soil (Macherey-Nagel) kit ...
-
bioRxiv - Immunology 2022Quote: For RNA extraction NucleoSpin RNA II kit (Macherey-Nagel) was used ...
-
bioRxiv - Cell Biology 2022Quote: ... Cellular RNA was purified with the NucleoSpin RNA Plus Kit (Macherey-Nagel). Organ tissues were homogenized with Precellys® Tissue Homogenizer and purified with TRIzol™ Reagent (Invitrogen) ...
-
bioRxiv - Biochemistry 2022Quote: ... an appropriate volume of Protino® NiNTA-Agarose (MACHEREY-NAGEL) slurry was washed with Desalting buffer ...
-
bioRxiv - Molecular Biology 2022Quote: ... The amplified product was cleaned by using the PCR Clean Up Kit (#740609.50; Macherey-Nagel), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons (typically 2.5 mL) were purified using NucleoMag NGS Clean-up and Size Select (Macherey-Nagel) following manufacturer’s instructions and eluted in 450 μL distilled water ...
-
bioRxiv - Molecular Biology 2022Quote: ... Extractions were performed using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) following the manufacturer protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... each pool purified (Macherey-Nagel™ NucleoSpin™ Gel and PCR Clean-up Kit) and send to GENEWIZ Germany GmbH for Illumina sequencing (Amplicon-EZ Sequencing Service ...
-
bioRxiv - Biophysics 2022Quote: ... Plasmid purifications for mammalian cell lines transfection were performed using high-yield column-based commercial NucleoBond Xtra Midi kit (Macherey-Nagel, Germany) following usage protocols.
-
bioRxiv - Microbiology 2022Quote: ... coli stock using the Nucleospin plasmid kit (Macherey-Nagel, Germany) and the PCR amplicon used was generated using C ...
-
bioRxiv - Genomics 2022Quote: ... then genomic DNA was isolated using the Sodium Dodecyl Sulfate (SDS) procedure of the NucleoSpin Plant II DNA isolation kit (Macherey-Nagel, Dueren, Germany). Preparation of DNA libraries for bisulfite sequencing was performed as described in (Nunn et al. ...
-
bioRxiv - Microbiology 2022Quote: ... Verified plasmids were purified using a Midiprep kit (Macherey-Nagel, 740412) and concentrated by standard sodium acetate/ethanol precipitation (REF Sambrook ...
-
bioRxiv - Microbiology 2022Quote: ... NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Bethlehem, PA) was used to remove contaminants ...
-
bioRxiv - Microbiology 2022Quote: ... Each female was held above a sterile microcentrifuge tube filled with 700 μl lysis buffer SL1 (NucleoSpin Soil kit, Macherey-Nagel, Düren, Germany), and the ovipositor was dipped into the liquid and moved in circles for one min to wash microbes off of the ovipositor’s surface (WS) ...
-
bioRxiv - Microbiology 2022Quote: DNA was extracted using the NucleoSpin Soil kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol with some modifications ...
-
bioRxiv - Immunology 2022Quote: ... coli hosts containing DCFHP genes were maxi-prepped (Macherey-Nagel). HD-BIOP3 cells were then transfected with both components and underwent selection by glutamine deprivation.9 Resulting pooled cells were sorted and resulting single clones were analyzed as described throughout the manuscript ...
-
bioRxiv - Cell Biology 2022Quote: ... genomic DNA (gDNA) was isolated using the XL Maxi NucleoSpin Blood kit (Macherey-Nagel) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2022Quote: RNA isolation was carried out using the NucleoSpin® RNA Plant Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... or NucleoSpin RNA Plant (Macherey-Nagel). First-strand complementary DNA was synthesized from 0.5 μg total RNA using a ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO).
-
bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
-
bioRxiv - Genetics 2022Quote: ... Total RNA was isolated using the NucleoSpin RNA Plant Kit (Macherey-Nagel), cDNA was synthesized using the PrimeScript RT Reagent Kit (Perfect Real Time ...
-
bioRxiv - Systems Biology 2022Quote: ... followed by the addition of 50 μL or 80 μL of N-methyl-N-[trimethylsilyl]trifluoroacetamide (MSTFA, Macherey-Nagel, Düren, Germany) and incubated at 37 °C for 60 min for plasma and urine ...
-
bioRxiv - Systems Biology 2022Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
-
bioRxiv - Microbiology 2022Quote: ... the NucleoSpin RNA Kit (Macherey-Nagel) was used ...
-
bioRxiv - Bioengineering 2022Quote: ... The cssDNA released from the phage were then extracted with NucleoBond Xtra Midi EF kit (Macherey-Nagel) following the manufacturer’ s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... 20 µL of proteinase K (20 mg/mL; MACHEREY-NAGEL # 740506) was added and the mixture incubated at 70°C for 20 min ...
-
bioRxiv - Bioengineering 2022Quote: ... 8×104 661W cells were seeded in a 24-well culture plate with DMEM and transfected with the plasmids containing guides using Lipofectamine™ Cells were harvested 72 hours following the transfection and Genomic DNA was then extracted using NucleoSpin® Tissue kit (Macherey-Nagel) following the manufacturer’s recommendation.
-
bioRxiv - Microbiology 2022Quote: DNA extraction was performed as described in [21] using the Nucleospin tissue kit (Macherey-Nagel). DNA concentration and purity were checked with a NanoDrop One (Thermo Scientific) ...
-
bioRxiv - Bioengineering 2022Quote: ... All constructs were verified by sequencing and purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s instructions.
-
bioRxiv - Bioengineering 2022Quote: Cultured cells were lysed in Macherey-Nagel RA1 buffer containing 1% β-mercaptoethanol and total RNAs were isolated using NucleoSpin RNA isolation kit (Macherey-Nagel 740955). Reverse transcription and PCR amplification were performed as described before21 ...
-
bioRxiv - Bioengineering 2022Quote: ... PCR products were purified using a NucleoSpin Gel and PCR purification kit (Macherey-Nagel) and indexed using a TruSeq Nano DNA Library Prep kit (Illumina ...