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Citations for Macherey-Nagel GmbH :
1551 - 1600 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Viral RNA in supernatant cell culture was isolated with the Nucleospin Dx virus (Macherey-Nagel). Reverse transcription was performed with random primers and Superscript IV (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: Total cellular RNA was extracted with the nucleospin RNA plus XS (Macherey-Nagel). Viral RNA in supernatant cell culture was isolated with the Nucleospin Dx virus (Macherey-Nagel) ...
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bioRxiv - Developmental Biology 2023Quote: ... The BAC DNA was purified using the NucleoBond Xtra BAC kit (Macherey-Nagel 740436.2) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting PCR products were purified using a NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel). dsRNA was generated using the MEGAscript T7 kit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... BM DOHH2 were resuspended in RA1 buffer and tris(2-carboxyethyl)phosphine (TCEP) from Macherey-Nagel and stored at -80°C for less than 3 months ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was extracted (NucleoSpin RNA-purification kit, Macherey-Nagel Dueren, Germany) and retrotranscribed cDNA quantified using the Universal Human Probe Roche library (Roche-Diagnostics ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmids were prepared using a NucleoBond Xtra Maxi kit (Macherey-Nagel) and eletroporated ex-vivo as described by 83 ...
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bioRxiv - Evolutionary Biology 2023Quote: Bulk DNA of the meristematic region and the basal portions of the leaves was extracted using NucleoSpin Plant II kit (Macherey-Nagel, Germany). DNA concentration was determined using a Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... They have been further purified using the Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al. 2011). The extracts were subsequently diluted in water and quantified with a Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Genetics 2023Quote: ... CGN22793 was isolated using a NucleoSpin® RNA plant kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: RNA was extracted using the Nucleospin RNA XS kit (740902.10, Macherey-Nagel # 740902). Extracted RNA was used to synthesize cDNA by reverse transcription using the SuperScript III kit (Thermo Fisher 18080044 ...
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bioRxiv - Cell Biology 2023Quote: ... or from supernatants using NucleoSpin RNA virus kit (Macherey-Nagel). Reverse transcription and quantitative real-time PCR was performed using Luna Universal One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted from wild-type worms using the Nucleospin RNA® kit (Macherey-Nagel – 740955) or Trizol Reagent™ (Ambion® ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled library plasmids were purified using NucleoBond PC 500 Maxi kit (Macherey-Nagel). The same protocol was applied to generate the library for the second round of selection ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted using NucleoSpin (Macherey-Nagel™ NucleoSpin™ RNA, Mini Kit) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... supernatant was removed and cell pellets were used for plasmid isolation by using NucleoBond□ Xtra Midi Plasmid DNA Purification Kit (Macherey-Nagel) according to the protocol supplied by the kit.
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bioRxiv - Bioengineering 2023Quote: ... The ligation was purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) and used for the electroporation of ElectroMAX DH5α-E Competent Cells (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... The thin layer chromatography was done with silica gel on aluminum foils (60 Å pore diameter) obtained from Macherey-Nagel and visualized with ultraviolet light (λ = 254 and 365 nm) ...
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bioRxiv - Biochemistry 2023Quote: ... The preparation was purified using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). The DNA preparation was used for transformation into chemically competent E ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids were isolated using the NucleoSpin Plasmid Kit (Macherey-Nagel) and sequenced by Eurofins using pT7-7_Seq_F and pT7-7_Seq_R as sequencing primers (Table S4) ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: ... Total DNA was extracted using NucleoSpin blood mini kit (MACHEREY-NAGEL). sgRNA was amplified by PCR using mixed P5 primer and P7 primer with i7 index sequence ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA of tick organs and eggs was extracted using the NucleoSpin Tissue kit according to the manufacturer’s instructions (Macherey-Nagel, Germany). DNA was eluted with 50 μL of elution buffer and was stored at -20°C until use.
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bioRxiv - Molecular Biology 2023Quote: ... RNA was purified using the Nucleospin RNA kit (Macherey-Nagel). Assessment of rRNA integrity and RNA-sequencing was performed by the Genome Technology Access Center at Washington University in St ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Three independent PCR reactions from the same library were combined after PCR and purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing was carried out on an Illumina HiSeq XTen at Macrogen ...
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bioRxiv - Bioengineering 2023Quote: ... gDNA was obtained using the NucleoBond HMW DNA kit (Macherey-Nagel) as per manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... The protein samples resolved in agarose gel were vacuum-transferred onto the nitrocellulose membrain (Porablot, MACHEREY-NAGEL), and subjected to a standard Western-Blotting procedure with either anti-Sup35NM ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting amplicons were pooled and gel purified using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Samples were sequenced on a MiSeq platform with ∼10 million reads per sample (Illumina) ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from skeletal muscle using NucleoSpin RNAXS kit (Macherey-Nagel). Single-stranded cDNA was synthesized from 1 μg of total RNA using the High Capacity RNA-to-cDNA Kit (Applied biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid DNA was extracted and purified with Nucleobond Xtra Maxi EF kit (Macherey-Nagel) and used to produce lentiviruses with an average titer of 109 TU/ml ...
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bioRxiv - Bioengineering 2023Quote: ... 83 RNA was extracted from MCF7 cultures (NucleoSpin RNA, Macherey-Nagel) and the quality was checked using a NanoDrop One spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from fresh bacterial cultures using the NucleoSpin® 96 Tissue Core Kit (Macherey-Nagel, Germany). All DNA samples were sequenced at the Max Planck-Genome-Centre Cologne using the Illumina paired-end technology with read lengths of 150 bp ...
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bioRxiv - Plant Biology 2023Quote: The RNA extraction and conversion to cDNA of tomato leaves was carried out using columnkit based on silica membranes (Macherey-Nagel XXX GmbH, Germany) following the manufacturer’s protocol ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from the input sample and beads by adding 300 μl buffer A1 (NucleoSpin RNA kit, Macherey-Nagel) and 300 μl absolute ethanol followed by RNA extraction according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: RNA was isolated from T-REx-293 and A549 cells using the NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel). 500 ng of total RNA was used for cDNA synthesis using Maxima™ Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: Protein samples were run on bis-acrylamide gels and then transferred to PVDF membranes (Macherey-Nagel, Düren, Germany) using a Trans-blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Medium pH was evaluated by using a pH test paper Duotest pH 5.0-8.0 (Macherey-Nagel). We found that pH was not altered by lactate addition up to 20 mM (data not shown).
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel., Düren, Germany). COI gene fragments were amplified from some samples according to the USDA recommended protocol (https://www.fsis.usda.gov/sites/default/files/media_file/2020-11/CLG-FPCR.pdf ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: RNA was extracted from cultured cells using a NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cssDNA released from phage were then extracted with NucleoBond Xtra Midi EF kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA extraction was performed using NucleoSpin RNA kit (Macherey-Nagel) including DNase treatment according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and 300 mg (JFC) of both fresh and treated frass (n = 3) using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) and following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were subsequently purified using the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2023Quote: ... slight adaptations were made from manufacturer’s instructions: half MCE filters were placed into individual 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel) and added with 1,200 µL MWA1 lysis buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... or the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... HPLC-grade acetonitrile was purchased from Macherey-Nagel. Formvar carbon film on 200-mesh copper grids (FCF200-Cu ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Microbiology 2023Quote: ... If no genomic DNA could be obtained strains were cultured according to the manufacturer’s protocol and DNA was extracted using the NucleosSpin Plasmid (NoLid) Mini kit (Macherey-Nagel cat. No. 740499) with vortexing after addition of BufferA2 and BufferA3 ...
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bioRxiv - Microbiology 2023Quote: ... viminalis roots using the Nucleo Spin® Soil kit (Macherey-Nagel, Germany). Quantitative determination of the concentration (ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... VII-1-1 and IX-5-2 (Genomic DNA extraction kit, Macherey-Nagel, Hoerdt) by using the primers listed in Table S4 ...