Labshake search
Citations for Macherey-Nagel GmbH :
1551 - 1600 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... extracted with chloroform and the NucleoSpin RNA Mini kit (Macherey-Nagel). Total RNA from sorted cells was isolated with the Rneasy Plus Micro Kit (Qiagen ...
-
bioRxiv - Genomics 2022Quote: ... The PCR products were gel extracted using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Next ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmids were purified with the Nucleospin plasmid purification kit (Macherey-Nagel). Linear DNA was purified from agarose gels using the gel extraction kit from Qiagen ...
-
bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted by the NucleoSpin® RNA Plus Kit (Macherey-Nagel, Germany) according to the appropriate protocol ...
-
bioRxiv - Plant Biology 2022Quote: Leaf material of soil grown plants was collected and homogenized in liquid nitrogen prior to extraction of RNA with the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s advice ...
-
bioRxiv - Biochemistry 2022Quote: Plasmids and polymerase chain reaction (PCR) products were purified using kits from Macherey-Nagel. PCR fragments were amplified using Q5 High-Fidelity 2x Master Mix (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Amplified cDNAs were purified using PCR/Gel purification column (Macherey-Nagel #740609). 500ng of PCR amplicons were fragmented and tagged with Illumina DNA Prep (Illumina #20060060) ...
-
bioRxiv - Biochemistry 2022Quote: ... Total RNA was extracted using the NucleoSpinR RNA Plus kit (Macherey-Nagel GmbH& Co KG, Germany) followed by further purification (NucleoSpinR RNA Clean-up kit ...
-
bioRxiv - Biochemistry 2022Quote: ... VP Nucleodur 100-5C 18 ec column (250 ×40 mm, 7 μm: Macherey-Nagel) used as stationary phase ...
-
bioRxiv - Biochemistry 2022Quote: ... The preparation was purified using Protino Ni-NTA agarose (MACHEREY-NAGEL, Germany) in a gravity-flow chromatography column at 10 °C after membrane solubilization with 1.5 % (w/v ...
-
bioRxiv - Bioengineering 2022Quote: ... DNA was purified from PCR mixtures or agarose gels using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). The purity and concentration of DNA were checked by NanoDrop 2000 (Thermo Fisher Scientific) ...
-
bioRxiv - Biophysics 2022Quote: ... Products were then run on a 1% agarose gel and the gel was extracted using NucleoSpin (Macherey-Nagel, 740588.50) clean up kit according to manufacturer specifications.
-
bioRxiv - Cancer Biology 2022Quote: ... and cleaned using the NucleoSpin© Gel and PCR clean-up kit (Macherey-Nagel, cat. no. 740609) following manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and their DNA was extracted using a Nucleospin Tissue kit (Macherey-Nagel). Libraries were prepared separately for each of the four individuals ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... genomic DNA was extracted using a Nucleospin Tissue kit (Macherey-Nagel). After quality control ...
-
bioRxiv - Biochemistry 2022Quote: ... and column purified (cat no 740.948, Macherey-Nagel), following manufacturer’s protocols ...
-
bioRxiv - Biochemistry 2022Quote: ... The proteins were purified from the cleared lysate with Ni-NTA agarose (Macherey-Nagel, Düren, Germany) following concentration and dialysis against 0.1 M potassium phosphate buffer pH 7.
-
bioRxiv - Biochemistry 2022Quote: ... pre-tRNAPheGAA2-2 and human pre-tRNATyrgta8-1 and tRNAArgtct3-2 were amplified by PCR of respective pUC19 vectors and template DNA was isolated using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). RNA substrates were produced by run-off in vitro transcription using T7 RNA polymerase (New England Biolabs ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mini it for RNA purification (#740955.50, Macherey-Nagel), and cDNA was synthesized using iScript Reverse Transcription Supermix (#1708841 ...
-
bioRxiv - Biophysics 2022Quote: ... The product was then purified from 1% agarose gel and extracted using the NucleoSpin clean up kit (Macherey-Nagel, 740588.50).
-
bioRxiv - Bioengineering 2022Quote: ... The extraction of iDNA was performed using the NucleoSpin® PowerSoil kit (Macherey-Nagel, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... the RNA was further purified using the NucleoSpin RNA kit (Macherey-Nagel) according to manufacturer’s protocol.
-
ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... Correct clones were determined by sequencing and cultured for subsequent plasmid preparation using NucleoBond Xtra Midi/Maxi kits (Macherey-Nagel GmbH & Co. KG). For scaled CFPS reactions ...
-
bioRxiv - Biochemistry 2022Quote: ... coli using NucleoBond Xtra Maxi columns (Macherey-Nagel) followed by isopropanol precipitation ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... and small (<200 nucleotides) and large (>200 nucleotides) RNA were extracted separately for each tissue with the NucleoSpin® miRNA kit (Macherey-Nagel; Düren, Germany). RNA quality and quantity were determined using a NanoDrop 2000 (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... The amplicons were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) following the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from mid-log yeast cultures grown 16 h at 20°C in YPD as described above using the Nucleospin RNA II kit (Macherey-Nagel) and were reverse transcribed with Superscript-II reverse transcriptase (Invitrogen) ...
-
bioRxiv - Biochemistry 2022Quote: ... To purify the DNA the PCR NucleoSpin® Gel and PCR Clean-up-kit (Macherey-Nagel) was used according to manufacturers’ manual except that the elution was done in 140 µl 1xTE ...
-
bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from cell pellets using the NucleoSpin Blood XL Kit (Macherey-Nagel). The sgRNA sequence was amplified by PCR with sufficient gDNA to maintain representation ...
-
bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
-
bioRxiv - Biophysics 2022Quote: ... NiNTA beads were given into a empty Protino column (Macherey-Nagel, Düren, Ger, 745400.10) and washed again ...
-
bioRxiv - Biophysics 2022Quote: ... LifeactmScarlet-6His was bound to Protino NiNTA Agarose (Macherey-Nagel, Düren, Ger, 745400.25) and washed (50 mM Tris/Cl pH 8.0 ...
-
Driving Osteocytogenesis from Mesenchymal Stem Cells in Osteon-like Biomimetic Nanofibrous ScaffoldsbioRxiv - Bioengineering 2022Quote: ... total RNA was extracted using NucleoSpin RNA Set for NucleoZOL (Macherey-Nagel) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2022Quote: ... DNA was purified with NucleoSpin gDNA Clean-up Kit (Macherey-Nagel), following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... the three vectors were amplified using the NucleoBond Xtra Midi kit (Macherey-Nagel), and a combination of the glCV30 Hc and glCV30 Lc vectors or a combination of the glCV30-SDF-1α Hc and glCV30 Lc vectors were introduced into the CHO-S cells (Gibco) ...
-
bioRxiv - Biophysics 2022Quote: ... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
-
bioRxiv - Biophysics 2022Quote: ... and 6 µg total endotoxin free plasmid DNA (4 µg HaloTag-KIF5B1-560 plasmid DNA + 2 µg of α-tubulin-GFP) isolated using the NucleoBond Xtra Midi Plus EF kit (MACHEREY-NAGEL GmbH & Co ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from snap frozen mouse or human liver tissue using NucleoSpin RNA Mini kit (Macherey-Nagel, Düren, Germany). First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen) ...
-
bioRxiv - Cell Biology 2022Quote: ... Transcription reactions were purified (NucleoSpin RNA Clean-up, Macherey-Nagel) and annealed in 3x soaking buffer (32.7 mM Na2HPO4 ...
-
bioRxiv - Cell Biology 2022Quote: ... PCR products were purified (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and used as templates for T3 and T7 transcription reactions (MEGAscript ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from zebrafish larvae using Nucleospin XS micro kit (Macherey-Nagel, #740902.50) following manufacturers instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Precleared lysates were incubated with Ni-NTA beads (Macherey-Nagel) for 1 h at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 10% SDS-polyacrylamide gels and transferred onto a 0.45 µm pore size nitrocellulose membrane (Macherey-Nagel GMBH: 741280) by wet blotting ...
-
bioRxiv - Cell Biology 2022Quote: ... and EBs at day 5 using the NucleoSpin RNA Plus Kit (Macherey-Nagel, Düren, Germany) and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
-
bioRxiv - Cell Biology 2022Quote: Genomic DNA was isolated from iPSCs and EBs using the NucleoSpin Tissue Kit (Macherey-Nagel, Düren, Germany) with the manufacturer’s instructions and quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from iPSCs and EBs using the NucleoSpin RNA Plus Kit (Macherey-Nagel, Düren, Germay), quantified with a NanoDrop ND-2000 spectrophotometer (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: Total RNA was isolated from E2 (HH12) quail embryos using NucleoSpin RNA Plus (Macherey-Nagel) and reverse-transcribed using PrimeScript II reverse transcriptase (Takara Bio) ...
-
bioRxiv - Developmental Biology 2022Quote: ... The plasmids were purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel) and dissolved in TE (10 mM Tris-HCl pH 8 ...
-
bioRxiv - Cell Biology 2022Quote: ... fecal RNA was purified using the same methodology and Nucleospin Triprep kit (Macherey-Nagel, 740966.10) as in the Sugisawa study ...
-
bioRxiv - Cell Biology 2022Quote: Genomic DNA was extracted from liver using the Genomic DNA from organs and cells Kit (Macherey-Nagel) following manufacturer’s recommendations ...