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Citations for Macherey-Nagel GmbH :
1601 - 1650 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the NucleoSpin RNA Virus (Macherey-Nagel GmbH) or by semiautomated RNA extraction using the Easymag (BioMerieuxTM) ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from strains using NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany). The preparation of the transposon mutant library for Oxford Nanopore Sequencing has been done as described by Linder et al ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... DNA isolations were conducted using the NucleoSpin Plant II Mini Kit for DNA from plants (Macherey-Nagel, Düren, Germany). Preliminary quality assessments were conducted using agarose gel visualization and NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was extracted from ∼300 mg of soil using the NucleoSpin Soil 96 kit (Macherey-Nagel, Düren, Germany). Lysis was performed using the Buffer SL with Enhancer SX and on the FastPrep 24 homogenizer (MP Biomedicals ...
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bioRxiv - Synthetic Biology 2023Quote: ... or NucleoSpin Plasmid kit (Macherey-Nagel). The correct assembly of all plasmids was verified by Sanger sequencing (Microsynth Seqlab).
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bioRxiv - Synthetic Biology 2023Quote: ... or the Nucleospin gel and PCR clean-up kit (Macherey-Nagel). Restriction and ligation reactions were performed using enzymes and buffers obtained from New England Biolabs and Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... The column was an EC150/4.6 NUCLEODUR C18 gravity column (Macherey-Nagel GmbH and Co. KG, Germany), the mobile phase was Acetonitrile:0.2 % (v/v ...
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bioRxiv - Synthetic Biology 2023Quote: ... peptides were desalted by using C18 solid phase extraction cartridges (Macherey-Nagel). Cartridges were prepared by adding acetonitrile ...
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bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
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bioRxiv - Systems Biology 2023Quote: ... and 230 μg of DNA was extracted using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). Efficiency of the re-circularization was checked by Sanger sequencing of eight clones ...
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bioRxiv - Systems Biology 2023Quote: ... The pellet was washed once with sterile H2O and then each library was purified over two columns of the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). A total of 17 Mio were harvested ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted using either a NucleoSpin Tissue Kit (Macherey-Nagel) or the phenol-chloroform protocol [50] ...
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bioRxiv - Genomics 2023Quote: ... with 300 µl of Lysis Buffer T1 (Macherey-Nagel, Düren, Germany). The insect was homogenized with a pestle using a vertical motion ...
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bioRxiv - Genetics 2023Quote: ... DNA was extracted using the NucleoSpin 96 Plant II Kit (Macherey-Nagel, Germany), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... After adding 30 µl N-methyl-N-trimethylsilyl-trifluoroacetamide (Macherey-Nagel), samples were incubated for 30 min at 45°C under continuous shaking.
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated using an extraction kit according to the manufacturer’s instructions (Macherey-Nagel). The RNA concentration was assayed with a NanoDrop (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: Total RNA was prepared from isolated colonic epithelial cells of Hsp60Δ/ΔIEC and Hsp60fl/fl mice on day 2 with the column-based NucleoSpin RNAII kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from 50mg of root using the NucleoSpin® RNA Kit (Macherey-Nagel). cDNAs were synthesized with the M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Genetics 2022Quote: ... DNA was then extracted from tissue using the NucleoSpin Tissue Mini kit columns and corresponding protocol for extraction from animal tissue (Macherey-Nagel, Duren, Germany). For each sperm pool ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid purification was performed using NucleoBond Xtra Midi EF Kit from Macherey-Nagel. Inserts were sequenced at Eurofins MWG Operon ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: RNA was extracted from purified astrocyte preparations using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250). RNA was analyzed using TapeStation ...
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bioRxiv - Neuroscience 2022Quote: RNA from purified astrocytes or brain cell suspension after enzymatic digestion was extracted using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250), genomic DNA was eliminated and cDNA was synthetised (Thermo Fisher Scientific #K1672) ...
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bioRxiv - Neuroscience 2022Quote: ... purified (Macherey-Nagel #740416.10), and packaged into AAV8 vector (packaging was done by University of Helsinki AAV core unit) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA extracts were obtained using the Nucleospin RNA kit (Macherey-Nagel) and cDNAs were synthetized using SuperScript III reverse transcriptase (Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... total RNA extracts were obtained using the Nucleospin RNA kit (Macherey-Nagel) and cDNAs were synthesized using SuperScript III reverse transcriptase (Thermofisher Scientific) ...
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bioRxiv - Bioengineering 2022Quote: ... RNA extraction was carried out using the nucleospin RNA kit (Macherey-Nagel). The RNAs were quantified using a Nanodrop and the retro-transcription made using the high-capacity cDNA reverse transcription kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... extracellular viral RNA was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and intracellular viral RNA using the E.Z.N.A Total RNA Kit I (Omega Bio-tek) ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA in each sample was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and quantified by qRT-PCR.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse cross-linked chromatin was purified using the NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were isolated using an endotoxin-free plasmid midiprep kit (Macherey-Nagel) for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50 ...
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bioRxiv - Cell Biology 2022Quote: DNA from electroporated HSC was extracted using the tissue XS kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The resulting PCR product was ‘cleaned up’ using a NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Capped and Poly(A ...
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bioRxiv - Biochemistry 2022Quote: ... Coli using NuceloBond Xtra Maxi Kit per the manufacturers recommendations (Macherey-Nagel). All scFv-ACE2 ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Bioengineering 2022Quote: ... The remaining cuticular wax-chloroform mixture was filtered (cellulose folded filter paper; 4-12 µm pore size, M&N 615 1/4, Ø 185 mm, Macherey-Nagel GmbH & Co. KG), and chloroform was evaporated (rotary evaporator ...
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bioRxiv - Bioengineering 2022Quote: ... Plasmid extraction and polymerase chain reaction (PCR) purification kits were ordered from Macherey-Nagel GmbH & Co ...
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bioRxiv - Bioengineering 2022Quote: ... purified using PCR clean-up kit (Macherey-Nagel), and transformed in electrocompetent E ...
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bioRxiv - Bioengineering 2022Quote: ... Derivatization was done using 20 µl N,O-bis(trimethylsilyl)-trifluoroacetamid (BSTFA; Macherey-Nagel, Düren, Germany) and 20 µl pyridine (Sigma Aldrich Corp. ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was isolated using NucleoSpin RNA II Kit (Macherey-Nagel) in pursuance of the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... TEV-protease and unprocessed hsTANGO1 were removed by Protino-Kit (Macherey-Nagel). Flowthrough and wash fractions were collected ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from wt and Uhrf1- or Usp7-knockdown BHK cells using the nucleospin triprep kit from Macherey-Nagel. 500 ng of total RNA was reverse transcribed with a high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were centrifuged at 21000 g for 10 min and supernatants were transferred to silica membrane spin columns (Macherey-Nagel) and centrifuged at 11000 g for 1 min ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated using NucleoSpin mini RNA kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from cells using a NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was isolated with Macherey-Nagel− NucleoSpin− Blood kit (740951.50, Macherey-Nagel) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... the DNA was purified using NucleoSpin columns (Macherey-Nagel) and sequenced on a NextSeq 500 Illumina sequencer.