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Citations for Macherey-Nagel GmbH :
1501 - 1550 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The complete mitochondrial genome of Spurilla braziliana MacFarland 1909 (Nudibranchia, Aeolidiidae)bioRxiv - Genomics 2023Quote: ... Japan (Figure 1) and preserved in NucleoProtect RNA (MACHEREY-NAGEL) for 5.5 months at -80 °C before DNA extraction ...
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bioRxiv - Genomics 2023Quote: Total RNA was extracted from immature inflorescences (stages 1-12) or aerial parts of 16- day-old seedlings using NucleoSpin RNA Plus kit (Macherey-Nagel) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated with Nucleozol™ (Macherey-Nagel, Düren, Germany) following the extraction protocol suggested by the manufacturer ...
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bioRxiv - Synthetic Biology 2023Quote: ... Synthons were purified with the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) following the manufacturer’s protocol using a vacuum manifold ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from the youngest leaf tissues of three biological replicates of DM-susceptible and DM-resistant yellow maize inbred lines (mock-inoculated and DM-inoculated) using the modified NucleoSpin™ Mini Kit for RNA purification (Macherey-Nagel™, GmbH & Co. Du ren, Germany). One hundred fifty (150 ...
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bioRxiv - Microbiology 2023Quote: ... the PCR product was purified using a NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel) and subjected to Sanger sequencing at Eurofins Genomics GmbH (Ebersberg ...
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bioRxiv - Microbiology 2023Quote: ... Chromatographic separations were carried out on a reversed-phase C18 column (50×2.1 mm, 1.8-µm particle size; Macherey-Nagel GmbH, Dueren, Germany) using a CH3OH:H2O (both supplemented with 0.1% acetic acid ...
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bioRxiv - Genomics 2023Quote: ... DNA were extracted from individual clones using Nucleobond Xtra midi kit (Macherey-Nagel, Düren, Nordrhein-Westfalen) and 2 μg of each individual BAC clone were used for PacBio library preparation.
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bioRxiv - Genomics 2023Quote: ... gDNA was isolated using the NucleoSpin Blood Mini Kit (Macherey-Nagel). The gDNA concentrations were quantitated by Qubit.
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bioRxiv - Genetics 2023Quote: ... All probes were gel purified using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel, cat# 740609). The pixel intensities of the bands corresponding to linear ...
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bioRxiv - Genomics 2023Quote: ... Amplification products were cleaned up using NGS clean-up magnetic beads (Macherey-Nagel), introducing a size selection through a concentration of 1.2x beads per sample and eluted in a total amount of 25 µL EBT buffer (1 mM EDTA ...
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bioRxiv - Genetics 2023Quote: ... DNA was purified using NucleoSpin PCR clean-up kit (Macherey-Nagel, cat# 740609), dissolved with ddH2O and adjusted to a concentration of 0.6 μM ...
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bioRxiv - Microbiology 2023Quote: ... manual protocol 5.2 (Macherey-Nagel; Düren, Germany).
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bioRxiv - Genomics 2023Quote: High molecular weight DNA was extracted from blood cells using a NucleoBond AXG column (Macherey-Nagel, Düren, Germany), which was followed by purification with phenol-chloroform ...
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bioRxiv - Microbiology 2023Quote: ... DisA was incubated with unlabeled ATP and 15 µl of the reaction product was analysed by thin layer chromatography (TLC; POLYGRAM CEL300 PEI plates) (Macherey-Nagel GmbH & Co. KG, Düren, Germany) using a running buffer of [1 vol ...
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bioRxiv - Genomics 2023Quote: ... DNA was isolated using the NucleoMag Plant kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using the Nucleospin RNA kit (Macherey-Nagel, Germany) followed by a RVFV-specific RT-qPCR amplification [32] in a Lightcycler L96 (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The cloned BAC DNA was extracted using NucleoBond Xtra BAC kit for large construct plasmid DNA (Macherey-Nagel, catalog number 740436.25). For library cloning ...
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bioRxiv - Genetics 2023Quote: ... PCR reactions were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel).
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bioRxiv - Immunology 2023Quote: ... RNA from tumours was extracted and purified from tumours using the NucleoSpin RNA extraction kit (Macherey-Nagel). Library preparation and sequencing were performed by Genewiz (Leipzig ...
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bioRxiv - Genetics 2023Quote: ... the fragments were purified using a PCR/Gel purification kit (NucleoSpin Gel and PCR Clean□up, Macherey-Nagel) and sequenced using the smt0-seq-Rv oligonucleotide ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA was extracted using the NucleoSpin Plant II kit (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Purified cells were lysed and RNA was isolated using the NucleoSpin RNA XS kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... and NucleoMag® DNA/RNA Water kit for water and air sample (Macherey-Nagel, Hoerdt, France), referred to as the “Water” ...
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bioRxiv - Molecular Biology 2023Quote: ... In vitro transcribed RNA was purified using the NucleoSpin RNA Clean-up kit (Macherey-Nagel). RNA concentration and purity was evaluated using the NanoDrop One (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... NucleoSpin Soil kit (Macherey-Nagel) was used on 2.5 g sample following the kit instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
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bioRxiv - Microbiology 2023Quote: ... followed by the NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel, Düren, Germany). The second 16S amplification was performed with the primers 799 forward (5’-AACMGGA TTA GAT ACC CKG-3’ ...
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bioRxiv - Microbiology 2023Quote: ... and 16S amplicons (500 bp amplicon size) were purified by agarose gel separation using the NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel). Bacterial 16S amplifications were obtained using the FastStart High-Fidelity PCR system (Roche ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted in triplicate for mixed cell suspensions using either NucleoMag® DNA/RNA Water (Macherey-Nagel, Hoerdt, France), i.e. ...
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bioRxiv - Microbiology 2023Quote: ... The filters were then individually placed into 5 mL Type A Bead-Tubes (ref. 740799.50, Macherey-Nagel), added with 1,200 µL MWA1 lysis buffer and stored at - 80°C until nucleic acids extraction as specified in the corresponding section below.
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bioRxiv - Microbiology 2023Quote: The nucleic acids were extracted from the FFPE tissue sections using the Nucleospin total RNA FFPE XS (Macherey-Nagel). RNA sequencing libraries were prepared using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel). PCRs were performed with Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of the sample with gRNA–TAR and –p24 combined was run on a 1% agarose gel containing ethidium bromide and the two expected bands were cut out separately and purified using the Nucleospin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s manual ...
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bioRxiv - Genomics 2023Quote: ... and construct isolation with the NucleoSpin Plasmid kit (Macherey-Nagel, REF 740588.250), plasmids were verified with sanger sequencing.
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bioRxiv - Molecular Biology 2023Quote: ... or Nucleobond Xtra Midi (Macherey-Nagel, Germany) and each construction was verified by Sanger sequencing (Mix2seq ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and processed using the NucleoSpin RNA kit (Macherey-Nagel) or the PureLink RNA Mini Kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The correct bacterial cultures were processed the following day using the NucleoBond Xtra Midi kit for transfection-grade plasmid DNA (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and extracted with the NucleoBond High Molecular Weight DNA Kit (Macherey-Nagel, Düren, Germany). The extraction yielded 16.5 μg of high-molecular weight DNA ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was isolated from cells using the NucleoSpin Tissue kit (Macherey-Nagel). 500 ng-1 μg of genomic DNA was bisulfite converted using the EZ DNA Methylation-Gold kit (Zymo) ...
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bioRxiv - Microbiology 2023Quote: ... DNA purification used the Nucleospin® extract II kit (Macherey-Nagel®), according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: Total RNA was extracted from murine splenocytes using NuceloSpin RNA plus kit (Macherey-Nagel, France) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed in 350 µl of RA1 buffer of the NucleoSpin RNA kit (Macherey-Nagel) supplemented with 1 % beta-mercaptoethanol followed by passing ten times through a narrow-bore syringe (Omican-F ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was isolated from peripheral blood and tail tissues of each chimeric mouse 6 weeks after BM transplantation with NucleoSpin® Tissue Kit (MACHEREY-NAGEL, Duren, Germany). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... The library plasmids were extracted using the NucleoBond Xtra Maxi EF Kit (Macherey-Nagel) to avoid endotoxin contamination ...
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bioRxiv - Cancer Biology 2023Quote: Cells were washed with PBS and mRNAs were extracted using NucleoZOL (MACHEREY-NAGEL, 740404.200) according to the manufacturer’s instructions but the step with water was repeated twice to avoid contamination by genomic DNA ...
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bioRxiv - Developmental Biology 2023Quote: ... homogenized and frozen on dry ice in 350 μl of Buffer RA1 containing BME (Macherey-Nagel Nucleospin RNA isolation kit 740955), and stored at –80°C ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted using NucleoSpin Plus RNA kit (Macherey-Nagel). A total of 1.5 µg RNA was reversely transcribed using 0.5 μl Moloney murine leukemia virus reverse transcriptase (M1701 ...
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bioRxiv - Cancer Biology 2023Quote: RNA was isolated using the Nucleospin RNA plus kit (Macherey-Nagel, cat# 740955.50). Library generation was performed using KAPA RNA HyperPrep Kit with RiboErase (HMR ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was isolated (Macherey-Nagel, 740420.50) and the sequences were checked with sequencing (LGC Genomics GmbH ...