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Citations for Macherey-Nagel GmbH :
1501 - 1550 of 3058 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... 2 x 109 cells were used for extraction of genomic DNA using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). PCR-free libraries (microbial short insert libraries ...
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bioRxiv - Plant Biology 2022Quote: Total RNAs were extracted from leaves using NucleoSpin RNA Plant (MACHEREY-NAGEL) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted desulfoglucosinolates were separated on a reversed phase C-18 column (Nucleodur Sphinx RP, 250 x 4.6 mm, 5µm, Macherey-Nagel, Düren, Germany) with a water (A)-acetonitrile (B ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel), cDNA was synthesized using the PrimeScript RT Reagent Kit (Perfect Real Time ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted from the cells with a PGTX solution42 and purified using NucleoSpin RNA (MACHEREY-NAGEL). cDNA was synthesized from 1 μg of total RNA using ReverTra Ace qPCR RT Master Mix (TOYOBO) ...
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bioRxiv - Plant Biology 2022Quote: ... NucleoSpin RNA Plant (MACHEREY-NAGEL) was used for total RNA extraction from roots or shoots ...
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bioRxiv - Plant Biology 2022Quote: ... DNA was extracted from the distant rhizosphere using a modified Griffiths’ protocole that co-extracts DNA and RNA (as described above) and from the root and close rhizosphere using NucleoSpin Plant II kit (Macherey-Nagel). DNA was sent for 16S rRNA and ITS sequencing on an Illumina MiSeq in pair-ended mode (2×250 ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA was extracted using Nucleospin Plant RNA kit (#740949.250 Macherey-Nagel). RNA was used to generate Lexogen Quantseq 3’ RNA seq libraries at Cornell University Institute of Biotechnology Genomics Facility ...
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bioRxiv - Plant Biology 2022Quote: ... the adeD PCR product was additionally gel-purified (NucleoSpin Gel and PCR Clean-Up Kit, Macherey-Nagel, Düren, DE), and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... The culture medium was centrifuged at 6000 rpm for 20 min and then vacuum-filtrated through a filter paper (MN 615, Macherey-Nagel) and subjected to a two-step cation exchange chromatography protocol using an ÄKTA Explorer FPLC system (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... purified with the NucleoSpin RNA Clean-up XS (Macherey-Nagel GmbH & Co. KG, Düren, Germany) and then used as the template for reverse transcription with the ReverTra Ace qPCR RT kit (Toyobo ...
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bioRxiv - Plant Biology 2022Quote: ... and sealed with an N 9 PP screw cap with a center hole and silicone white/PTFE red septum (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Plant Biology 2022Quote: ... purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and circularized using T4 DNA ligase.
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bioRxiv - Plant Biology 2022Quote: ... Plasmids were extracted from positive colonies using 5 mL liquid cultures with the NucleoSpin Plasmid Purification kit (Macherey-Nagel; Ref.: 11932392) and sequenced (GATC ...
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bioRxiv - Plant Biology 2022Quote: ... the rhizosphere and root subpools were purified on an agarose gel (amplicon ∼450 bp) using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany), whereas all other subpools were purified with CleanNGS beads ...
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bioRxiv - Neuroscience 2022Quote: ... 100 µL of homogenate was set aside as total cell (T) and mixed with NucleoZOL (Macherey-Nagel, #740404.200) before storage at -80°C ...
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bioRxiv - Neuroscience 2022Quote: ... the pellet was resuspended in 500 µL of NucleoZOL (Macherey-Nagel, #740404.200) before storage at - 80°C.
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bioRxiv - Neuroscience 2022Quote: ... total RNA was extracted according to NucleoZOL (Macherey-Nagel, #740404.200) manufacturer instructions and immediately processed as follows ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was amplified by PCR methods using the following oligo-DNA primers and the products were purified with micro spin columns (MACHEREY-NAGEL, 74060910). Each reverse primer also possesses T3 sequence for transcription.
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bioRxiv - Plant Biology 2022Quote: ... with the difference that a Nucleodur 100-5 NH2 125x4 mm column (Macherey-Nagel, #760730.40) and an eluent consisting of 8.5% acetonitrile and 25mM formic acid pH 4 were used (18) ...
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bioRxiv - Plant Biology 2022Quote: RNA was extracted using the RNA NucleoSpin Plant kit (Macherey-Nagel) according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of 5 days old seedlings (both controls and treated with NaCl) using the NucleoSpin RNA Plus (Macherey-Nagel). Reverse-transcription was performed using using the SuperScript III First-Strand VILO cDNA Synthesis Kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
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bioRxiv - Genetics 2022Quote: ... a NucleoSpin Plant II kit (Macherey-Nagel) for the Seven-parent-MAGIC and NORFAB panels ...
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bioRxiv - Genomics 2022Quote: ... After 20 hours at 30 ºC bacteria were harvested and plasmid preparations were performed using Macherey-Nagel™ NucleoBond™ Xtra Midi EF (Macherey-Nagel #740420.50).
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bioRxiv - Microbiology 2022Quote: ... The PCR product was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and cloned into the plasmid pCM009 (Schell et al. ...
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bioRxiv - Microbiology 2022Quote: ... Cloning was performed according to standard protocols and plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel). DNA fragments were amplified using Phusion High Fidelity DNA polymerase (NEB ...
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bioRxiv - Microbiology 2022Quote: ... coli clones using a Miniprep kit (Macherey-Nagel, 740588) and verified by single pass primer extension sequencing (ACGT Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... concentrated using a PCR cleanup kit (Macherey-Nagel, Düren, Germany), and run on the Illumina NextSeq 500 sequencing platform (Illumina Inc. ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was isolated from microdissected specific brain areas with NucleoSpin RNA kit (Macherey-Nagel)) or from neurons growing in MC-CS with an RNeasy Mini kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: Cells were lysed (RLT buffer with 1% β-mercaptoethanol) and RNA was isolated via column purification (NucleoSpin RNA kit, Macherey-Nagel 740955). RNA integrity and concentration were determined with BioAnalyzer (Agilent ...
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bioRxiv - Physiology 2022Quote: ... Human keratinocyte RNA was purified using NucleoSpin RNA (Macherey-Nagel, Düren, Germany). Before the RNA preparation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reactions were monitored by thin layer chromatography (TLC) on silica gel (60 F, 0.2 mm, ALUGRAM Sil G/UV254 Macherey-Nagel) by visualization under 254 and/or 365 nm lamp ...
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bioRxiv - Neuroscience 2022Quote: ... the vector backbone was gel-purified using the NucleoSpin Gel and PCR Clean‐up kit (Macherey-Nagel). Oligonucleotides pegRNA3-1 and pegRNA3-2 (Appendix Tables S1 and S2 ...
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bioRxiv - Neuroscience 2022Quote: ... plasmids were produced using the NucleoBond Xtra Midi kit(Macherey-Nagel) and transfected using Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted from HEK293TN cells with the NucleoSpin RNA kit (Macherey-Nagel) and 1 μg RNA was reverse-transcribed with oligo-dT18 using the First Strand cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) and quantified with a Qubit Fluorometer and the Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA was extracted from pelleted cells using minipreps (NucleoSpin Plasmid EasyPure Kit - Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using Macherey-Nagel NucleoSpin DNA mini kit (Macherey-Nagel, 740952) according to manufacturer’ s protocol ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA of five corrected hiPSC clones and the naïve parental iPSC line was isolated using the Macherey-Nagel NucleoSpin RNA mini kit (Macherey-Nagel, 740955) according to manufacturer’ s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA was isolated with NucleoSpin Plasmid Mini Kit and extracted from an agarose gel with NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). All constructs were verified by restriction digest and Sanger sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... 4C libraries were purified with the “Gel and PCR clean up” kit from Macherey-Nagel using NTI solution diluted 6 times and an elution buffer pre-heated at 70°C ...
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bioRxiv - Molecular Biology 2022Quote: ... gDNA wasisolated using the Nucleospin Plant II kit from Macherey-Nagel. Isolated genomic DNA was G-tailed at random DNA breaks using dGTP together with terminal transferase (NEB cat M0315S ...
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bioRxiv - Neuroscience 2022Quote: mRNA was isolated using NucleoSpin RNA XS kit (Macherey-Nagel, 740902.50) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR product was purified with NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany), and sequenced by Eurofins Genomics ...
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bioRxiv - Microbiology 2022Quote: ... The nucleoSpin plasmid easypure Kit (Macherey-Nagel) was used for plasmid purification and expression cassettes were sequence verified ...
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bioRxiv - Microbiology 2022Quote: ... caecal and colonic tissue was isolated by using the NucleoSpin RNAII kit (Macherey-Nagel GmbH) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... or NucleoSpin® RNA Virus (Macherey-Nagel GmbH, Düren, Germany). BHK-21 cells were infected with VSV-G-3CLpro-L wild type and F305L (3CLpro ...
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bioRxiv - Microbiology 2022Quote: ... The 0.22 µm membrane was used for bacterial DNA isolation using NucleoMag DNA/RNA Water Kit (MACHEREY-NAGEL Inc., Bethlehem, PA) following manufacturer’s instructions ...