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Citations for Macherey-Nagel GmbH :
1851 - 1900 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... Ligated DNA constructs were separated with agarose gels from other reaction products and purified by gel extraction (NucleoSpin, Macherey-Nagel) by avoiding any exposure to ethidium bromide or UV light.
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bioRxiv - Genomics 2022Quote: ... Maxiprep was performed with the Nucleobond Xtra endotoxin-free maxiprep kit (Macherey-Nagel).
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from cell pellets using the RNA extraction kit (Macherey-Nagel) and 1-2 µg of RNA was reverse transcribed into cDNA using GoScript (Promega).
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bioRxiv - Biophysics 2022Quote: ... coli MC1061 cell line using the Nucleobond Xtra Maxi Kit (Macherey-Nagel). 1.3 μg DNA was used per 106 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... The library stock was prepared by scraping plates followed by purification using the Midi Prep kit (Macherey-Nagel). Library sequencing was performed on a MiSeq run to ensure sgRNA uniformity ...
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bioRxiv - Cancer Biology 2022Quote: ... and excised from a 0.8% agarose gel for purification using the NucleoSpin Gel Cleanup Kit (Macherey-Nagel). The insert pool and digested backbone were then ligated overnight at 16°C using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2022Quote: RNA was extracted from retinal samples using the NucleoSpin® RNA kit (Macherey-Nagel) using the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with the NucleoSpin RNA XS Kit (Macherey-Nagel, Düren, Germany). To avoid contamination with its food Tetraselmis sp. ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted right after sorting using the NucleoSpin® RNA kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Molecular Biology 2022Quote: Viral RNA isolation was performed with the NucleoSpin RNA Virus kit (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... (2009) and treated with rDNase (Macherey-Nagel GmbH, Düren, Germany). 500ng of total RNA was used for cDNA synthesis using ProtoScriptII reverse transcriptase ...
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bioRxiv - Biochemistry 2022Quote: ... PCR products were visualized on a 1% TAE agarose gel and purified using the Nucleospin PCR Clean-up Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... NucleoSpin miRNA Plasma Kit (NUC; Macherey-Nagel, 740981.50), QIAamp ccfDNA/RNA Kit (CCF ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA was isolated using the Macherey-Nagel NucleoBond Xtra Midi Plus (Macherey-Nagel, Germany). The sgRNAs were amplified and adaptors attached in a single PCR step ...
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bioRxiv - Cancer Biology 2021Quote: ... The 3C template was purified using Nucleomag PCR beads (“P-beads”, Macherey-Nagel #744100.1), eluted in 450 μl 5mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA fragments were purified with NucleoSpin gel and PCR clean-up columns (Macherey-Nagel, Düren, Germany) and were Sanger- sequenced using their corresponding primers by VBC Biotech (Vienna ...
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bioRxiv - Microbiology 2021Quote: ... Fecal gDNA was extracted using the Nucleospin DNA stool kit (Macherey-Nagel) according to the manufacturer’s instructions with the following modification ...
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bioRxiv - Cancer Biology 2021Quote: ... Amplified libraries were purified using a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) and size selected by electrophoresis on non-denaturing 6% TBE-Urea gels ...
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bioRxiv - Cancer Biology 2021Quote: ... using the Nucleospin Tissue kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Cells were treated as indicated for 72 hours prior to harvesting of RNA using the Nucleospin RNA kit (Macherey-Nagel). First-strand cDNA synthesis was performed using iScript Supermix (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... with a VP 250/10 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel, Düren, Germany) running a general gradient of 10% to 70% acetonitrile (ACN ...
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bioRxiv - Biochemistry 2021Quote: ... with an EC 250/4 NUCLEODUR 100-5 C18 ec column (Macherey-Nagel) with a general gradient of 10% to 90% ACN in H2O ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from cells using NucleoSpin RNA isolation kit (Macherey-Nagel, Düren, Germany) or GenElute Mammalian Total RNA Miniprep kit (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2021Quote: ... total RNA was isolated from cell lines using NucleoSpin RNA Kit (Macherey-Nagel) and subjected to Real-Time PCR using the high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... suspended in PI solution (25 μg/ml PI solution in PBS with RNAase A; MACHEREY-NAGEL, #740505). and incubated for 10 minutes on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were extracted and purified using a Midi-Prep Kit (Macherey-Nagel). The correct insertion of full-length TCF7L1 cDNA ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel, Germany). 1 µg of total RNA was reverse-transcribed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Genomic DNA was extracted using Nucleospin Blood XL kit (Macherey-Nagel cat# 740950) and eluted into 1ml elution buffer ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from total thymocytes and from sorted T-cell subsets from MCMV-infected and uninfected mice according to the manufacturer’s instructions using the NucleoSpin Blood QuickPure kit (MACHEREY-NAGEL), and stored at −20°C until further analysis.
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bioRxiv - Immunology 2021Quote: ... and isolated from the gel with the gel extraction kit (Macherey-Nagel). The amplicons were A-tailed and cloned with T/A cloning into a Golden Gate compatible version of pBluescript II SK(- ...
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bioRxiv - Immunology 2021Quote: ... Positive clones were identified by colony PCR and the plasmids were isolated using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel, Düren, Germany). The DNA sequence of the cloned devil CIITA transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
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bioRxiv - Immunology 2021Quote: ... RNA extraction (two replicates per cell line) was performed using the Nucleospin® RNA Plus Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in RNA lysis buffer and RNA was isolated using the NucleoSpin RNA Plus RNA isolation kit (Macherey-Nagel). For some experiments ...
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bioRxiv - Immunology 2021Quote: ... Virus was purified by centrifugation at 12,000 xg for 10 mins at 4 °C and genomic DNA extracted (NucleoSpin Tissue kit, Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Immunology 2021Quote: ... Genomic DNA from infected MCL cells was extracted using the NucleoSpin Tissue kit (Macherey-Nagel). PCR reactions were performed using KAPA HiFi polymerase (KAPA biosystems ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was filtered using a 0.45 µm syringe filter and loaded onto a 5 ml Protino Ni-NTA column (Macherey-Nagel, Düren, Germany). The column was washed until UV280 had reached a steady value and eluted with 500 mM imidazole ...
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bioRxiv - Genetics 2021Quote: ... and used for extraction of total RNA with the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). Total RNA was reverse transcribed to cDNA using the PrimeScript cDNA synthesis kit (Takara Bio ...
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bioRxiv - Genetics 2021Quote: ... genomic DNA was extracted using NucleoSpin tissue kit (Macherey-Nagel, Düren, Germany) and amplified by PCR using specific primers (Table S2 ...
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bioRxiv - Genetics 2021Quote: ... DNA was extracted following using NucleoSpin columns (Macherey-Nagel) and eluted in 30 µL of NE buffer ...
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bioRxiv - Genetics 2021Quote: ... followed by purification (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Membranes were washed twice with 2x SSC ...
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bioRxiv - Genetics 2021Quote: ... (NucleoSpin Tissue Column - Macherey-Nagel). PiggyBac constructs AGG1906 (Pxmeiw68-Cas9 Genbank accession ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR fragments were extracted from agarose gels (Macherey-Nagel, 740609.250). pBlueRabbit vector was digested with EagI and XbaI ...
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bioRxiv - Genetics 2021Quote: ... DNA from three female and three male fifth instar larvae was isolated individually using the NucleoSpin DNA Insect kit (Macherey-Nagel). We observed that the primers Masc_F1 and Masc_R1 targeting EkMasc and EkMascB consistently showed off-target amplification in female samples ...
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bioRxiv - Genomics 2021Quote: ... DNA were extracted from individual clone using Nucleobond Xtra midi kit (Macherey-Nagel) and used for PacBio library preparation by The French Plant Genomic Resources Center (CNRGV ...
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bioRxiv - Genetics 2021Quote: ... kuehniella larvae in triplicate using the NucleoSpin DNA Insect kit (Macherey-Nagel, Düren, Germany) as described elsewhere [57] ...
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bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Autosampler vials and caps and the silylation reagents N-trimethylsilylimidazole (TSIM) and N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) were purchased from Macherey-Nagel (Düren, Germany). The primary secondary amine (PSA ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was collected from the DT40 cells using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co., Germany) and subjected to complementary DNA synthesis and quantitative PCR using a ReverTra Ace qPCR RT Kit (Toyobo Life Science ...