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Citations for Macherey-Nagel GmbH :
1851 - 1900 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Total RNA was extracted using the NucleoSpin®RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Total RNA from one hundred thousand TILs was extracted using a commercially available RNA isolation kit (Macherey-Nagel) following the manufacturer’s recommendations and ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were further treated with rDNase (cat no 740.963, Macherey-Nagel) and column purified (cat no 740.948 ...
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bioRxiv - Bioengineering 2022Quote: ... Genomic DNA (gDNA) was extracted using a genomic DNA extraction kit (Macherey-Nagel). Primers were designed to amplify approximately 400-700 bp of the region targeted by the guide RNAs (Supplementary Table 5) ...
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bioRxiv - Biochemistry 2022Quote: ... and viral RNA from cell lysate was extracted using the Nucleospin RNA plus mini kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... using the Nucleospin virus kit (Macherey-Nagel). Purified DNA was subsequently used for PCR amplification with the following primers ...
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bioRxiv - Synthetic Biology 2022Quote: ... and LA1737 and LA1738 (629 bp downstream of stop codon) (Table S11) from genomic DNA prepared from our Liverpool WT colony using the NucleoSpin Tissue kit (Macherey-Nagel) and ligated into the plasmid sequentially by standard restriction enzyme-based cloning to generate bgcn-Cas9 (AGG1207).
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bioRxiv - Neuroscience 2022Quote: ... transfection-grade plasmids were produced using midi-prep kits (NucleoBond Xtra Midi EF; Macherey-Nagel, Düren, Germany).
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bioRxiv - Molecular Biology 2022Quote: RNA was isolated using NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG). The hypothalami were transferred to tubes with 1.4 mm ceramic beads (Omni International) ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were cut out and purified with the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... the DNA plasmid was extracted using a NucleoBond® Xtra Midi kit (MACHEREY-NAGEL #740410.50). The efficiency of transformation was controlled by plating 0.01% of the transformation reaction to a 15 cm LB agar plate with 100 µg/mL ampicillin.
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bioRxiv - Cancer Biology 2022Quote: ... the genomic DNA was extracted using NucleoSpin® Tissue kit (MACHEREY-NAGEL; #740952.50) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... and nodules using a NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and isolation was performed by mechanical disruption and chemically followed by NucleoSpin gDNA Clean-up Kit (Macherey-Nagel). RNA was removed with ribonucleases and genomic DNA concentration was quantified with NanoDrop ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA was extracted using NucleoSpin plant II kit from Macherey-Nagel following the instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... or MidiPrep (Macherey-Nagel) kits and verified using sequencing services provided by GeneWiz ...
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bioRxiv - Cell Biology 2022Quote: ... and purified using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). In vitro transcription reaction was carried out in a final volume of 100 μl transcription mix containing 80 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified from agarose gels using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). Single-stranded RNA of positive and negative polarity was synthesized by in vitro transcription and purified as described above ...
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bioRxiv - Immunology 2022Quote: RNA was isolated using the NucleoSpin RNA XS Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The surviving cells were expanded for 24 h and DNA was extracted using a NucleoSpin Tissue kit (Macherey-Nagel, Cat#740952). An additional eight biological replicates were similarly prepared but did not undergo killing by TC5A87 ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from 2 × 105 cells untreated or butyrate-conditioned BMDMs using the NucleoSpin RNA extraction kit (Macherey-Nagel, 740902.50) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... PCR products were first purified using NucleoSpin kit (Macherey-Nagel) followed by Ampure bead purification (Beckman Coulter) ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was isolated from the lung using NucleoSpin® RNA (Macherey-Nagel, Düren, Germany), and then converted to cDNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and 2.5μL of RNAse (Macherey-Nagel) at 1000 unit and 3.5 mg/mL ...
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bioRxiv - Microbiology 2022Quote: ... capillary column coated with a silarylene stationary phase (MACHEREY-NAGEL GmbH & Co., Germany). Helium was applied as carrier gas with 1.5 ml/min and a pressure of 12.319 psi (0.8494 bar) ...
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bioRxiv - Microbiology 2022Quote: ... Filter pieces were transferred into MN Bead Tubes Type A (Macherey-Nagel) containing 800 μl of buffer RLT Plus that were pre-chilled at −80°C for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted using NucleoSpin RNA XS (MACHEREY-NAGEL). RNA quality and quantity were measured using a 2100 Bioanalyzer System (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was extracted using NucleoSpin RNA XS (MACHEREY-NAGEL). We used 10 ng of total RNA per 15 μl reaction for the RT-PCR reaction with each assay primer (Gapdh ...
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bioRxiv - Developmental Biology 2022Quote: The RNA form resected fin pieces was extracted using NucleoSpin RNA Plus XS kit for RNA purification (Macherey-Nagel) according manufacturers protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA were extracted using the NucleoSpin RNA kit (MACHEREY-NAGEL) and their quality assessed by NanoDrop™ 1000 Spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... CNA35 (Macherey-Nagel) integrated to pET28a mCherry vector was used ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was collected from cells using the NucleoSpin RNA Kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was isolated using the NucleoSpin Tissue – Mini kit for DNA from cells and tissue (MACHEREY-NAGEL). Genomic DNA was sheared with a 30-G syringe ...
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bioRxiv - Immunology 2022Quote: ... and total RNA was extracted from hCD45+ cells using NucleoSpin® RNA kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... lymph nodes and bone marrow cells by manual extraction with the NucleoSpin® Tissue kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted using the NucleoSpin RNA extraction kit (Macherey-Nagel) as indicated by manufacturer guidelines ...
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bioRxiv - Immunology 2022Quote: ... RNA from dry pellets of Vγ9Vδ2 T lymphocytes was extracted and purified using the NucleoSpin RNA plus isolation kit from Macherey-Nagel. DGE-sequencing was performed by the BIRD core facility and data were analyzed using R programming languages and tools.
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bioRxiv - Developmental Biology 2022Quote: ... and purification was achieved using an Nucleobond Xtra Maxi EF kit (Macherey-Nagel 740424.10). Plasmids were verified by restriction and sequencing to confirm the correct insertion.
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bioRxiv - Microbiology 2022Quote: ... DNA was isolated from 35 strains using the NucleoSpin Tissue Kit (Macherey-Nagel) following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... using the NucleoSpin RNA virus kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... extracted with chloroform and the NucleoSpin RNA Mini kit (Macherey-Nagel). Total RNA from sorted cells was isolated with the Rneasy Plus Micro Kit (Qiagen ...
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bioRxiv - Genomics 2022Quote: ... The PCR products were gel extracted using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Next ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were purified with the Nucleospin plasmid purification kit (Macherey-Nagel). Linear DNA was purified from agarose gels using the gel extraction kit from Qiagen ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted by the NucleoSpin® RNA Plus Kit (Macherey-Nagel, Germany) according to the appropriate protocol ...
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bioRxiv - Plant Biology 2022Quote: Leaf material of soil grown plants was collected and homogenized in liquid nitrogen prior to extraction of RNA with the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s advice ...
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bioRxiv - Biochemistry 2022Quote: Plasmids and polymerase chain reaction (PCR) products were purified using kits from Macherey-Nagel. PCR fragments were amplified using Q5 High-Fidelity 2x Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplified cDNAs were purified using PCR/Gel purification column (Macherey-Nagel #740609). 500ng of PCR amplicons were fragmented and tagged with Illumina DNA Prep (Illumina #20060060) ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA was extracted using the NucleoSpinR RNA Plus kit (Macherey-Nagel GmbH& Co KG, Germany) followed by further purification (NucleoSpinR RNA Clean-up kit ...
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bioRxiv - Biochemistry 2022Quote: ... VP Nucleodur 100-5C 18 ec column (250 ×40 mm, 7 μm: Macherey-Nagel) used as stationary phase ...