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Citations for Macherey-Nagel GmbH :
1901 - 1950 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Fragments of 300–2000 bp were excised from the gel and isolated with a gel-extraction kit (Macherey-Nagel, NucleoSpin Gel and PCR Clean-up ...
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bioRxiv - Molecular Biology 2021Quote: All plasmids were prepared using the NuceloSpin Plasmid Transfection-grade kit (MACHEREY-NAGEL, 740490.250). Plasmids were transfected with Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was isolated using the RNA isolation kit (MACHEREY-NAGEL, 740984.250). Synthesis of cDNA was done using the SuperScript™ IV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplicons were purified using the NucleoSpin® Gel and PCR clean up kits from Macherey-Nagel (Bethlehem, PA), after which quantification and qualification of purified band(s ...
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bioRxiv - Molecular Biology 2021Quote: ... was purified by NucleoSpin® Gel and PCR clean up (Macherey-Nagel, Bethlehem, PA) after which the nucleotide sequence determined by the Sanger method (Genewiz ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested and RNA was extracted following the NucleoSpin RNA Plus kit protocol (Macherey-Nagel) for qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... the total RNA was extracted using a Nucleo Spin RNA XS kit (MACHEREY-NAGEL). First-strand cDNA was synthesized from the total RNA using Prime Script Reverse Transcriptase (TAKARA Bio) ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel, Düren, Germany). The eluted protein was dialyzed against storage buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Harvested bacteria were lysed using a French press (3 runs at 20.000 psi) in 1× LEW buffer (Macherey-Nagel, Düren, Germany) containing 5% (v/v ...
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bioRxiv - Molecular Biology 2021Quote: ... The precipitated DNA was purified with NucleoSpin gDNA Clean-up Kit (Macherey-Nagel). Finally ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA from cultured cells was isolated using NucleoSpin®RNA (Macherey-Nagel), Düren ...
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bioRxiv - Developmental Biology 2021Quote: PCR products were purified using NucleoSpin PCR Clean-up procedure (Macherey-Nagel 740609.250) and mutations were confirmed by sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA from individual embryos was isolated with the NucleoSpin® Tissue XS kit (Cat#740901, Macherey-Nagel). A PCR reaction was performed using the isolated genomic DNA with primers flanking the CAS9 target site ...
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bioRxiv - Developmental Biology 2021Quote: ... digestion and purified using a NucleoSpin Gel and PCR Clean-up (#740609, Macherey-Nagel, Duren, Germany) kit ...
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bioRxiv - Developmental Biology 2021Quote: ... Genomic DNA was extracted with gDNA Nuclespin tissue kit (Macherey-Nagel) and the genotypes of the mice were determined from genomic DNA by using a PCR primer pair 1 (5’-TAAGTCTCTTGTTTGCTGTG-3’ ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein samples were extracted from the organs with the NucleoSpin RNA/Protein kit (Macherey-Nagel). Macherey-Nagel Bead Tubes Type F was used in tissue homogenization ...
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bioRxiv - Developmental Biology 2021Quote: ... GR-REV and GR-KAN by using Necleospin RNA Plant kit (Macherey-Nagel, www.mn-net.com). cDNA was made from 1 ug total RNA using Tetro cDNA synthesis kit with DNase treatment according to manufacturer’s instructions (Bioline) ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmids used for electroporation were purified using the Nucleobond Xtra Midi kit (Macherey-Nagel). Final concentration of DNA delivered per embryo for electroporation is between 1 to 2μg/μl.
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bioRxiv - Developmental Biology 2021Quote: ... the DNA was purified by column purification (Gel and PCR clean up kit, Macherey-Nagel) and used for RNA synthesis (Megashortscript T7 Kit ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The NaOH was neutralized by the addition of 1 M HCl and the cDNA was purified by a PCR-clean-up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... NucleoSpin® Microbial DNA kit (Macherey-Nagel) was used for the extraction of genomic DNA aimed for long-read sequencing ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Genomic DNA for short-read sequencing was extracted using NucleoSpin® Tissue kit (Macherey-Nagel, GmbH & Co. KG, Germany), library was prepared by Nextera® XT Library Preparation kit (Illumina ...
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bioRxiv - Evolutionary Biology 2021Quote: We used NucleoSpin Tissue kits (Macherey-Nagel, Düren, Germany) to extract and purify genomic DNA from the hind femur of each individual ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The NucleoSpin DNA RapidLyse Kit (Macherey-Nagel) was used for the DNA extraction from the Queensland Museum archival specimen QM_G335164 ...
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bioRxiv - Evolutionary Biology 2021Quote: Genomic DNA was extracted using the Nucleospin® plant II (Macherey-Nagel) following the manufacturer’s protocols ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to pellet the cell debris and the RNA was extracted from the supernatant using the two column-system of the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Germany). DNA removal was ensured by the on-column DNA digestion according to the manufacturer’s description ...
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bioRxiv - Evolutionary Biology 2021Quote: RNA was extracted from the thawed pellets using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... purified using Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al., 2011) following the supplier’s instructions with a two-fold elution step and finally quantified with Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Total RNA was isolated from heart ventricles using a NucleoSpin RNA Plus isolation kit (Macherey-Nagel, #740984) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: For tissue extraction the RNA purification Kit (Macherey-Nagel) was used ...
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bioRxiv - Neuroscience 2021Quote: ... Transcripts were purified using the RNA Nucleospin Clean up kit (Macherey-Nagel, Bethlehem, PA). The transcribed NeuroD1 was injected at 1 ...
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bioRxiv - Neuroscience 2021Quote: ... After adding 30 µl N-methyl-N-trimethylsilyl-trifluoroacetamide (Macherey-Nagel), samples were incubated for 30 min at 45 °C under continuous shaking.
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Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... All cRNA samples were purified with the NucleoSpin RNA Clean-up system (Macherey-Nagel, GmbH & Co. KG, Düren, Germany) and quantified on a Nano-Drop spectrophotometer ND-2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: RNA was purified using the Nucleospin gel and PCR clean-up kit from Macherey-Nagel. Complementary DNA (cDNA ...
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bioRxiv - Neuroscience 2021Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... and total RNA was isolated using NucleoSpin RNAXS kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted using NucleoSpin RNA Plus Kit (740984.50 Macherey-Nagel GmbH & Co. KG, Germany). Complementary DNA was prepared with SuperScript® III First Strand Synthesis SuperMix (11752-050 ...
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bioRxiv - Molecular Biology 2021Quote: ... total DNA was extracted using the NucleoSpin Tissue kit from Macherey-Nagel (#740952) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA In vitro transcription templates were purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The in vitro transcripts (IVTs) were cleaned up using the NucleoSpin RNA Clean-Up Kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions and subsequently quantified by using a Qubit RNA BR Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was prepared from frozen cell pellets by using NucleoSpin® RNA (MACHEREY-NAGEL) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel, Inc). The High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA was isolated and purified using the NucleoSpin Tissue Kit (MACHEREY-NAGEL, cat# 740952). Purified DNA was sheared using the Covaris S220 ultrasonicator with microTUBE AFA (Covaris ...
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bioRxiv - Molecular Biology 2021Quote: ... amplified and purified (Macherey-Nagel). Plasmid concentrations were measured using a Nanodrop (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... plasmids were isolated with NucleoSpin Plasmid QuickPure kit (Macherey-Nagel) and suspended in water ...
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bioRxiv - Molecular Biology 2021Quote: ... Total mRNAs were extracted with the NucleoSpin RNA XS kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and a NucleoSpin RNA isolation kit (Macherey-Nagel, Germany). Hundreds of worms were collected for each experiment ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation and ZAP-S was captured using Ni-NTA resin (Macherey-Nagel). After elution with 500 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... P1-P4 were purified P1-P4 containing cultures by solid phase extraction with reversed phase C18 columns (Chromabond, Macherey-Nagel, Düren, Germany). Supernatants were acidified to pH 3 with HCl ...
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bioRxiv - Immunology 2021Quote: ... coli cultures and purified using Nucleobond Xtra maxi EF columns (Macherey-Nagel) according to manufacturer’s instructions ...