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Citations for New England Biolabs :
4701 - 4750 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... Each PCR amplification was performed using Q5® High-Fidelity DNA Polymerase (New England Biolabs), and the products were purified using the EZNA Cycle Pure® Kit or the NEB Monarch® PCR & DNA Cleanup Kit and confirmed by sequencing (MCLab or Genewiz).
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bioRxiv - Molecular Biology 2019Quote: ... PCR was carried out using the manufacturer’s recommendations for Phire Hot Start II Polymerase (NEB) using 1 μl of DNA and a final primer concentration of 100nM each ...
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bioRxiv - Neuroscience 2019Quote: ... Extracted DNA was then either cloned using NEB PCR cloning kit (New England Biolabs, E1202S) or reamplified and purified using Zymo DNA Clean and Concentrator (Zymo research ...
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bioRxiv - Plant Biology 2020Quote: ... PCR on the templates was performed using Phusion High-Fidelity Polymerase (NEB, Ipswich, Massachusetts, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Circularized cDNA was the template for PCR amplification using Phusion DNA polymerase (New England Biolabs) with Illumina sequencing primers ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We first produced the DNA-template for T7-transcription using PCR with Taq polymerase (NEB) and primers 11 ...
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bioRxiv - Genetics 2019Quote: ... but using NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs, Ipswich, U.S.). Finally ...
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bioRxiv - Molecular Biology 2019Quote: ... and amplified by inverse-PCR with Phusion Hotstart II polymerase (New England Biolabs; Beverley, MA), using primers DA162 and DA163 [67] ...
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bioRxiv - Molecular Biology 2020Quote: ... we performed RFLP on 200 ng of MSP PCR-product using Hph1 (New England Biolabs) restriction enzyme accordingly to manufacture’s instruction ...
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bioRxiv - Cancer Biology 2019Quote: ... Oligonucleotides were amplified by PCR using the Q5 High-Fidelity DNA Polymerase (New England Biolabs) and the following primers ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequently inserted by PCR in the destination using high fidelity Phusion® (NEB, USA) or KAPA polymerases ...
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bioRxiv - Genomics 2020Quote: KlenTaq wild type gene was amplified using a high fidelity PCR (Q5 polymerase from NEB) from a stored plasmid ...
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bioRxiv - Biophysics 2021Quote: A 2.5 kbp DNA fragment was PCR amplified from the pUC19 plasmid (New England Biolabs) with a double digoxigenin-labeled primer (Biomers GmbH ...
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bioRxiv - Genetics 2021Quote: All PCRs were performed with Q5 High-Fidelity polymerase from New England Biolabs (NEB, M0492L). Standard subcloning protocols were used to generate all the DNA plasmids (see details below) ...
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bioRxiv - Genetics 2020Quote: ... PCR amplification was performed using Phusion 2X Master Mix HotStart Flex (New England Biolabs M0536L), 10µM primer pair (see Key Resources Table Oligonucleotides) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Q5 DNA Polymerase (NEB) with primer 8 & 9 or 10 & 11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 μL cDNA was used in a 30-cycle PCR using Taq DNA Polymerase (NEB) with primer 4 & 6 or 5 & 6 ...
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bioRxiv - Immunology 2021Quote: ... PCRs were performed with Phusion High-Fidelity DNA Polymerase (2 U/µL) (New England Biolabs) in a total volume of 50 μl ...
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bioRxiv - Genomics 2020Quote: ... After adding 30 µl PCR MM [25 µl 2x LongAmp Taq 2X Master Mix (NEB), 0.2 µl 100 mM forward primer ...
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bioRxiv - Genomics 2020Quote: ... cells were lysed and used for PCR amplification with LongAmp Taq DNA Polymerase (NEB, M0323). Insertion of the cassette into the correct locus was confirmed by visualizing on 1% E-gel (Invitrogen ...
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bioRxiv - Genomics 2021Quote: ... PCR fragments were labeled using 32P-γ-ATP with T4 polynucleotide kinase (New England, Biolabs). Protein–DNA complexes were incubated for 1h at room temperature in binding buffer containing 25 mM Tris pH 7.4 ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR product was cloned into TOPO vector PCR2.1 and restriction analysis with BsiEI (NEB) for 1 hour at 60 °C revealed a G4C2 repeat expansion estimated around 50 repeats ...
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bioRxiv - Bioengineering 2021Quote: ... Genomic PCR was performed according to the manufacturer’s instructions using Q5 Hot Start polymerase (NEB); primers are listed in online supplemental table S3 ...
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bioRxiv - Bioengineering 2020Quote: ... PCR conditions: 0.02 U/μL Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs), 1X Q5 reaction buffer ...
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bioRxiv - Bioengineering 2020Quote: ... they were PCR purified and assembled using a NEBuilder HiFi DNA Assembly (New England Biolabs) protocol ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using Q5® High-Fidelity 2× Master Mix (New England Biolabs, US). The point mutation in dCas9 to create H840A Cas9n was made using Q5® Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... or colony PCR using Q5® High-Fidelity 2× Master Mix (New England Biolabs, US) if a chromosomal region was targeted ...
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bioRxiv - Biochemistry 2021Quote: ... PCR reactions were carried out using Q5 High-Fidelity Polymerase (New England Biolabs, Ipswich, MA) per the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2021Quote: ... gBlocks were amplified by PCR using Q5 High-Fidelity DNA Polymerase from NEB (NEB #M0492L) and NEB Tm calculator tool was used to estimate the annealing temperature ...
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bioRxiv - Neuroscience 2020Quote: The introns were PCR amplified using Q5 Hot Start 2x Master Mix (New England BioLabs) with primers designed to contain homology arms to their respective targets ...
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bioRxiv - Microbiology 2021Quote: ... and PCR carried out using Q5® High-Fidelity 2X Master Mix (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was amplified by PCR using Phusion High Fidelity DNA Polymerase (M0530L; New England Biolabs) using the following primers:
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bioRxiv - Bioengineering 2021Quote: ... EP-PCR was performed using Taq polymerase and the standard Taq buffer (New England Biolabs) with imbalanced dNTP (0.2 mM dATP ...
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bioRxiv - Synthetic Biology 2021Quote: ... The resulting DNA templates were column purified using a Monarch PCR&DNA Cleanup Kit (NEB) and quantified by their absorption on a NanoPhotometer (Implen) ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR was performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, USA). After agarose gel electrophoresis the PCR products were extracted from the gel with the QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB). This cDNA product was then cloned into the mammalian expression vector pCAGGS for transient transfection experiments as previously described [51].
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bioRxiv - Microbiology 2021Quote: ... Point mutations were introduced by site-directed mutagenesis using Phusion High-Fidelity PCR kit (NEB) using primers listed in Table S1.
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bioRxiv - Cancer Biology 2020Quote: ... and 25 μl of NEBNext High Fidelity PCR Master 2X Mix (New Englarnd Biolabs, M0541L). The primer sequences and their description ...
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bioRxiv - Microbiology 2021Quote: ... All PCR steps were performed with Q5® High-Fidelity DNA Polymerase (New England Biolabs) and primers provided by IDT (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2019Quote: ... The obtained PCR fragments were assembled by Gibson assembly according to the manufacturer’s protocol (NEB). Sequence-confirmed plasmids were transformed in the ΔfumABC ΔglgA deletion strain ...
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bioRxiv - Microbiology 2021Quote: ... Polymerase chain reactions (PCR) were performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs), and gel-purified products (hph and the gene of unknown function with 73% homology to an AAC(2’)-IIa resistance gene ...
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bioRxiv - Molecular Biology 2021Quote: ... PCRs were performed using the Q5® High-Fidelity DNA Polymerase kit (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The reacted DNA was then purified using PCR clean-up kit (New England Biolabs®) and quantified using the nanodrop ...
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bioRxiv - Microbiology 2019Quote: The purified aptamer pool was then amplified by PCR with One Taq DNA polymerase (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... from all the samples and amplified by PCR using Q5 master mix (New England Biolabs) and forward-TGTGTCAATGGAACTCTTGAC and reverse-TCGTTTCGACACCGAAGCAG primers.
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bioRxiv - Biophysics 2020Quote: ... 2.5 kbp and 1.3 kbp DNA tethers were generated by PCR from pUC19 plasmid (NEB) with a double digoxigenin- or biotin-labeled primer on one side and a phosphoprimer on the other side ...
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bioRxiv - Cell Biology 2021Quote: ... The recovered DNA was analyzed by quantitative real-time PCR using LunaScript qPCR mastermix (NEB) and the ABI StepOnePlus qPCR machine and primers in Table 3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Genotyping PCRs were carried out using Q5 High-Fidelity DNA polymerase (New England Biolabs, #M0491) with an annealing temperature of 65°C ...
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bioRxiv - Neuroscience 2022Quote: ... Unc-103(e1597gf) coding sequences were then PCR-amplified from pKMC183 using Phusion polymerase (NEB), digested with NheI/MluI enzymes ...
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bioRxiv - Molecular Biology 2022Quote: ... and purification of the PCR fragments was done using the Monarch Gel Purification Kit (NEB). All fragments for plasmids cloning were amplified using Q5 DNA Polymerase (NEB ...