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Citations for New England Biolabs :
4751 - 4800 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... the PCUP1-Sup35N-Aß42 plasmid was linearised by PCR (Q5 high-fidelity DNA polymerase, NEB) with primers that remove the WT Aß42 sequence (primers MS_03-04 ...
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bioRxiv - Immunology 2022Quote: ... VP2 and VP3 with high-fidelity Phusion HiFi PCR Master Mix (NEB, Ipswich, MA, USA) using specific primers (S6 Table) ...
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bioRxiv - Biochemistry 2022Quote: ... and the PCR reactions were then treated with 200 U/mL of Exonuclease I (NEB) for 1 h at 37°C ...
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bioRxiv - Genetics 2022Quote: ... All PCRs were performed using a high-fidelity DNA polymerase (Phusion, New England Biolabs, F530S) and constructs generated by PCR were sequence verified by Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... The Monarch DNA Gel Extraction and Monarch PCR and DNA Cleanup kits (New England Biolabs) were used to clean PCR products according to manufacturers instructions ...
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bioRxiv - Biophysics 2022Quote: ... Libraries were quantified with quantitative PCR (NEBnext Library quantification kit, New England Biolabs, Ipswich, USA) and an Agilent Bioanalyzer High Sensitivity DNA chip ...
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bioRxiv - Genomics 2022Quote: ... and Phusion® High-Fidelity PCR Master Mix with HF buffer (New England Biolabs Inc). The amplification was performed in 50 μL PCR reactions that contained:
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bioRxiv - Developmental Biology 2022Quote: ... and the 3’ homology arm were produced by PCR (Phusion DNA polymerase, New England Biolabs). The 5’ and 3’ homology arm DNA fragments were amplified from genomic DNA prepared from vasa-Cas9 flies ...
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bioRxiv - Cell Biology 2022Quote: ... RAD18 knockout positive cells were confirmed with PCR using Q5 High Fidelity Master Mix (NEB), sanger sequencing ...
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bioRxiv - Cancer Biology 2021Quote: ... the PCR product was incubated for 4 hours with 2uL DpnI (NEB, Cat. No R0176), at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... PCR was performed using Q5© Hot Start high-fidelity DNA polymerase (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was digested with the restriction endonucleases BamH1 and Kpn1 (New England Biolabs) and purified (QIAquick® PCR Purification Kit ...
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bioRxiv - Synthetic Biology 2021Quote: ... pL0s were generated via PCR (Q5 and OneTaq hot-start polymerases, New England BioLabs (NEB)) followed by In-Fusion (Takara Bio ...
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bioRxiv - Molecular Biology 2019Quote: ... and the PCR product was ligated into pDRF1-GW using T4 ligase (New England Biolabs). Next ...
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bioRxiv - Molecular Biology 2020Quote: ... DNA was subjected to PCR using Quickload Taq 2X mastermix (New England Biolabs, Hitchin, UK). The reaction mixture contained 25 μL Quickload mastermix ...
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bioRxiv - Immunology 2020Quote: ... Each PCR reaction was performed using Q5 Hot Start High Fidelity 2X Master Mix (NEB). For the first round of PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... and all PCRs were carried out using Q5 High-Fidelity DNA polymerase (New England Biolabs). All DNA fragments were purified from agarose gel (Monarch DNA Gel Extraction kit ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products were produced with the Q5 High-Fidelity 2X Master Mix (New England Biolabs). All inserts were verified by sequencing.
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bioRxiv - Microbiology 2020Quote: ... The PCRs followed the NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) PCR protocol ...
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bioRxiv - Immunology 2020Quote: ... Each 50 μL PCR reaction contained Q5 High-Fidelity DNA polymerase and buffer (NEB #M0491), 15ng of lentiCRISPRv2-Brie ...
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bioRxiv - Biophysics 2021Quote: ... and 39x parS were produced by PCR using a high-fidelity polymerase (Phusion Polymerase, NEB) (see Table S1 for primer sequences) ...
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bioRxiv - Neuroscience 2020Quote: ... 25 μl of the NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S), and 5 µL of the Illumina PCR Primer Cocktail (PPC ...
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bioRxiv - Developmental Biology 2022Quote: ... Visible bands were cloned using the NEB PCR cloning kit (New England Biolabs, MA, USA) and sanger sequenced ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragments were PCR amplified using Q5® High-Fidelity DNA Polymerase (New England Biolabs) or Taq DNA Polymerase (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... Each PCR#2 reaction contained 25 µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5 µL of a unique Nuc PCR#2 Fwd Primer (10 µM) ...
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bioRxiv - Genomics 2022Quote: ... Each “PCR#1” reaction contained 25 µL of Q5 High-Fidelity 2X Master Mix (NEB), 2.5 µL of Nuc PCR#1 Fwd Primer (10 µM) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... sgRNA sequence was amplified by PCR using Phusion® Hot Start Flex DNA Polymerase (NEB). PCR product was purified using mi-PCR Purification Kit (Metabion) ...
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Recurrent but short-lived duplications of centromeric proteins in holocentric Caenorhabditis speciesbioRxiv - Evolutionary Biology 2022Quote: ... PCR was done on cDNA using Phusion High-Fidelity DNA Polymerase Kit (New England Biolabs) guidelines according to the manufacturer’s recommendations using primers for hcp-3 ...
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bioRxiv - Genomics 2022Quote: ... 30 cycles of endpoint PCR were performed using NEB Standard Taq (New England Biolabs, USA). PCR products (6 μL ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were produced with the Q5 High-Fidelity 2x Master Mix (New England Biolabs). Some fragments were ordered as gBlocks at IDT ...
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bioRxiv - Immunology 2022Quote: PCRs were done with the Q5 Hot-start 2× master mix (New England BioLabs, NEB), and cloning was performed using the Gibson Assembly 2× Master Mix (NEB ...
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bioRxiv - Immunology 2022Quote: PCRs were done with the Q5 Hot-start 2× master mix (New England BioLabs, NEB), and cloning was performed using the Gibson Assembly 2× Master Mix (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR product was purified and digested by PstI and HindIII enzymes (New England Biolabs). The plasmid pSEVA2213_bglC was isolated and then digested using the same restriction enzymes ...
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bioRxiv - Cell Biology 2019Quote: ... Cox8A-SNAP was amplified by PCR from pSNAPf-Cox8A (New England Biolabs, Ipswich, MA, USA) and subsequently integrated into the linearized plasmid by Gibson assembly ...
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bioRxiv - Microbiology 2019Quote: ... 50 ng DNA was used for PCR-amplification with Q5 High-Fidelity DNA Polymerase (NEB) targeting to the V3-V4 region of bacterial 16S rRNA gene ...
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bioRxiv - Molecular Biology 2019Quote: ... Enhancer variants were created using the inverse PCR method with Hot Start Q5 Polymerase (NEB) and primers available in Table 2 ...
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bioRxiv - Molecular Biology 2019Quote: ... adaptor ligation and PCR amplification with NebNext Ultra library preparation kit for Illumina (NEB #E7370S) following the manufacture protocol ...
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bioRxiv - Microbiology 2019Quote: ... Polymerase Chain Reaction (PCR) was conducted following standard conditions outlined by New England Biolabs (NEB) (Ipswich ...
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bioRxiv - Microbiology 2019Quote: ... 72°C for 60 seconds) in 50 µl reactions using Phusion PCR polymerase mix (NEB) containing 25 pmol each of the following ultramers (the protospacer-matching sequence is underlined) ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA of indicated genes were amplified by PCR using Q5 HF DNA polymerase (NEB, #M0491L) and the following specific primers ...
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bioRxiv - Bioengineering 2019Quote: The pcDNA3.1 NcadTL expression construct was generated by PCR using Phusion Polymerase (New England BioLabs) with pcDNA3.1-NCadTS as a template ...
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bioRxiv - Bioengineering 2019Quote: The pcDNA3.1 NcadVenus expression construct was generated by PCR using Phusion Polymerase (New England BioLabs) with N-Cadherin-EGFP (Adgene plamid #18870 ...
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bioRxiv - Genomics 2019Quote: cDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) using input amounts determined from qPCR ...
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bioRxiv - Genomics 2019Quote: cDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) using volumes determined from qPCR distributed across 5 replicates each ...
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bioRxiv - Genomics 2019Quote: ... gDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) with a biotinylated reverse-transcriptase primer Creb_Hand_RT_3 and a biotinylated primer specific to luciferase (Creb_Luc_Seq_R) ...
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bioRxiv - Cell Biology 2020Quote: ... PCR for cloning was performed with the Q5 polymerase (New England Biolabs, Ipswich, MA, USA). Electroporation ...
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bioRxiv - Synthetic Biology 2020Quote: ... pL0s were generated via PCR (Q5 and OneTaq hot-start polymerases, New England BioLabs (NEB)) followed by In-Fusion (Takara Bio ...
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bioRxiv - Molecular Biology 2020Quote: ... adapter ligation and PCR enrichment with NEBNext Ultra RNA Library Prep Kit (NEW ENGLAND BioLabs). RNA and cDNA concentrations were determined using Qubit RNA/DNA (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2019Quote: All PCRs were performed with Q5 High-Fidelity polymerase from New England Biolabs (NEB, M0492L). Standard subcloning protocols and HiFi DNA Assembly Cloning Kit (NEB ...
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bioRxiv - Microbiology 2020Quote: ... 700 bp upstream and downstream of crvA (VCA1075) were amplified by PCR (Q5 Polymerase, NEB) using V ...