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Citations for New England Biolabs :
4651 - 4700 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... His6-tagged GATE16 was generated via Q5 site-directed mutagenesis PCR (New England Biolabs), replacing the GST tag with the sequence MHHHHHHGS ...
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bioRxiv - Biochemistry 2024Quote: ... PCR was carried out using the Q5 high-fidelity DNA polymerase (New England Biolabs), the 5’ phosphorylated primer pairs and their corresponding annealing temperatures (Ta) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The PCR product was digested overnight using Fnu4HI (New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and amplified using Next High-Fidelity 2x PCR Master Mix (New England Biolabs #M0541) with Nextera DNA CD Indexes ...
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bioRxiv - Genomics 2024Quote: ... PCR amplifications were performed using phusion hot start flex 2X master mix (NEB, M0536S) and 20-100ng genomic DNA with the following conditions for HBG-sgRNA target (denaturation - 98°C for 3:00 ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product and Plasmid were subjected to restriction digestion by using Xho1 (NEB) and Hind III (NEB ...
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bioRxiv - Microbiology 2024Quote: ... after PCR amplification from Bacteroides fragilis ATCC 25285 (NCTC 9343) genomic DNA by NEB HiFi DNA assembly (Gibson Assembly) ...
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bioRxiv - Immunology 2024Quote: ... library fragments were amplified using 1x NEB Next PCR master mix (New England BioLabs) and 1.25 μM of custom Nextera PCR primers forward and reverse ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCRs were run with the high-fidelity Q5 DNA polymerase (New England Biolabs). The PCR products (pICOt and pICOt2 ...
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bioRxiv - Microbiology 2024Quote: ... The two PCR products were joined using NEBuilder (New England Biolabs, Ipswich, MA, USA). The resulting construct was named pHY1399 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The phosphorylated part amplicon and PCR amplified plasmid were ligated with T4 ligase (NEB) for 2 h at room temperature in a 4:1 ratio according to the manufacturer guidelines ...
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bioRxiv - Synthetic Biology 2021Quote: ... 50-µL PCR reactions contained 1x Phusion HF buffer (New England Biolabs; Ipswich, MA, USA), 200 µM dNTPs ...
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bioRxiv - Biophysics 2021Quote: ... 25 μL of PCR reaction was mixed with 1 μL of DpnI (NEB, cat#R0176) at 37°C for 4 hours ...
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bioRxiv - Genetics 2020Quote: ... and then the PCR reactions were treated with 200 U/ml of Exonuclease I (NEB) for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The cDNA was purified using the Monarch DNA and PCR Cleanup Kit (New England Biolabs). A region around an A-to-I editing site in BPNT1 was amplified by Q5 Hot Start High-Fidelity 2X Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR products were inserted between EcoRV and BglII using gibson assembly (New England Biolabs) in pEGFP-N1-Ecad vector ...
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bioRxiv - Cell Biology 2020Quote: ... The PCR product was subsequently gel purified and assembled using Gibson Assembly (New England BioLabs) into pSR406-ADH1 digested with EcoRI/XbaI (New England BioLabs).
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bioRxiv - Physiology 2021Quote: ... Libraries were quantified with quantitative PCR (NEBnext Library quantification kit, New England Biolabs, Ipswich, USA) and a Agilent Bioanalyzer High Sensitivity DNA chip ...
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bioRxiv - Genetics 2019Quote: PCRs were performed with the Q5 Hot-start 2x master mix (New England Biolabs (NEB)) and cloning was performed using the In-Fusion HD cloning kit (Takara Bio ...
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bioRxiv - Cell Biology 2020Quote: ... the pHRRA1-GFP (pKS85) (Table 4) plasmid was PCR-amplified with Phusion HF Polymerase (NEB), using primers designed using the QuikChange Primer Design tool (Agilent) ...
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bioRxiv - Cell Biology 2020Quote: All PCRs for cloning were performed with Phusion High Fidelity DNA polymerase (NEB, cat# M0530) using the default buffer (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... PCR or restriction digest products were mixed with 6x gel loading dye (New England Biolabs) and run at 100V for 30 minutes ...
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bioRxiv - Genetics 2021Quote: ... by PCR amplification of target locus followed by T7 Endonuclease I assay (New England Biolabs).
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bioRxiv - Genetics 2021Quote: ... PCR products spanning the gRNA-targeted site were digested with BfaI (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA products were then amplified for 15 cycles of PCR using longAmp Taq (NEB M0287S). During amplification PrepX RNAseq index barcode primers were added for each library to enable multiplexing ...
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bioRxiv - Genomics 2021Quote: ... PCR reactions were cleaned up using 0.9X NEBNext Sample purification beads (E7137AA, New England Biolabs) and eluted in 25μL of 0.1X TE ...
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bioRxiv - Neuroscience 2021Quote: Semi-quantitative PCR was performed using Phusion High-Fidelity DNA Polymerase (New England Biolabs, M0530L) and revealed with GelRed (Biotium ...
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bioRxiv - Microbiology 2019Quote: PCR mixtures were digested for 1 h at 37 °C with DpnI (New England Biolabs) to remove the template plasmids ...
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bioRxiv - Developmental Biology 2020Quote: ... fusion PCR and added them to the pWPXL vector with Gibson Assembly Master Mix (NEB). We then constructed final transfer vectors by inserting target cDNA after the P2A using Gibson assembly ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR products were produced with the Q5 High-Fidelity 2X Master Mix (New England Biolabs). All inserts were verified by sequencing ...
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bioRxiv - Immunology 2022Quote: ... Realtime PCR is conducted following manufacturer standard protocol (Luna Universal qPCR Master Mix, NEB Inc). Sybr green RT-PCR primers used in this study is listed in Table 1.
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bioRxiv - Microbiology 2022Quote: ... A PCR reaction (30 cycles) was run with Phusion® High-Fidelity DNA polymerase (NEB). The primer pairs im126/im127 and im137/im138 were used to target the reference gene pta (74 ...
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bioRxiv - Molecular Biology 2021Quote: ... The PCR product was digested with KpnI and XbaI (New England Biolabs, Ipswich, MA, USA) and ligated into pVaxI vector ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR product was digested with DpnI for 3h at 37°C (New England Biolabs) and then 5 µl was transformed into Stbl3 competent cells (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... The vectors were used as templates for PCR with Q5 High-Fidelity DNA polymerase (NEB) and the products were gel extracted and purified with the PureNA Biospin Gel Extraction kit (Research Instruments) ...
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bioRxiv - Genomics 2020Quote: ... Both transcripts were amplified by PCR using Phusion high-fidelity DNA polymerase (New England BioLabs), according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... two templates with T7 promoter sequences were generated by performing PCR amplification (Q5 Polymerase, NEB) off of GeCKO v2.0 plasmid DNA using H1T7 and H1R primers for probe 1 and primers T2RT7 and T2F for probe 2 (Supplemental Table 4) ...
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bioRxiv - Synthetic Biology 2019Quote: ... prior to amplification via PCR (Q5® High-Fidelity Polymerase, New England Biolabs, Ipswich, USA). Forward and reverse primers were phosphorylated separately in 50 µL total volume with 4 µM primer ...
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bioRxiv - Synthetic Biology 2019Quote: ... purified (column-based NEB Monarch®PCR & DNA Cleanup Kit; New England Biolabs, Ipswich, USA) and the DNA quantity/quality was measured by photometry (Spectrophotometer UV5Nano ...
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bioRxiv - Genetics 2019Quote: ... Three PCR steps were performed to amplify the DNA for sequencing using Phusion polymerase (NEB). First primers targeting the p427Gal vector were used to amplify DNA containing the N18 barcode sequences ...
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bioRxiv - Biophysics 2019Quote: ... 25 µL of PCR reaction was mixed with 1 µL of DpnI (NEB, cat# R0176), 1 µL of T4 PNK (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA templates for sgRNAs were generated by PCR amplification (Phusion DNA Polymerase; New England Biolabs) of ssDNA oligonucleotides (purchased from Integrated DNA Technologies ...
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bioRxiv - Genomics 2019Quote: ... and the NEBNext High Fidelity 2X PCR Master Mix (New England Biolabs; Ipswich, MA, USA). PCR reactions were cleaned up with one volume AmpureXP beads ...
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bioRxiv - Bioengineering 2019Quote: ... Site-directed mutagenesis PCR was made with Q5 Hot-Start High-Fidelity DNA Polymerase (NEB) to amplify the p15van2 plasmid incorporating the mutations ...
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bioRxiv - Genetics 2020Quote: Oligonucleotide libraries were re-suspended in nuclease-free water and amplified by emulsion PCR (NEB Q5 polymerase ...
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bioRxiv - Microbiology 2019Quote: ... Inserted DNA fragments were prepared using standard PCR protocols using Phusion polymerase (New England Biolabs). The pMCSG53 vector was linearized by cleavage of the cloning site with SspI ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR reaction contained 0.5 μl of 5 U/μl Taq polymerase (New England Biolabs), 0.5 μl dNTPs (10 mM) ...
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bioRxiv - Microbiology 2019Quote: ... The pdi1 ORF was amplified by PCR using Phusion HF DNA polymerase (New England Biolabs) with primers pdi1StartXmaI and pdi1StopNotI ...
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bioRxiv - Bioengineering 2019Quote: ... The 3.8 kb pRS413 vector backbone was PCR amplified from plasmid pRS413 (New England Biolabs) using primer pair RS413-Fw/RS413-Rev for all yeast assembly reactions ...
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bioRxiv - Genomics 2019Quote: ... SIC integration sites were enriched by PCR (NEBNext Ultra Q5 Master Mix, New England Biolabs) using 12 µL of the eluate with suitable pairs of adapter- and SIC-specific primers ...