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Citations for New England Biolabs :
4551 - 4600 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: Cloning procedures involving PCR were performed using Q5 High-fidelity polymerase (New England Biolabs) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... All PCR reactions were performed using the Phusion high fidelity DNA polymerase (NEB, M0530S). All the PCR-amplified fusion genes and epitope tags were cloned into the pSKP32 vector (Kaeser-Pebernard et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Correct assembly was verified by PCR using Taq 2X Master Mix (New England BioLabs) and Sanger sequencing (Genewiz).
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bioRxiv - Evolutionary Biology 2023Quote: ... and mutated by PCR to generate the disease variant alleles (New England Biolabs #E0554S). The utilized mutagenic primers are as follows ...
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bioRxiv - Bioengineering 2023Quote: ... DNA fragments were amplified by PCR (Q5 2x Master Mix, New England Biolabs (NEB)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR was performed using the Quick-LoadR©Taq2×Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Fragments were isolated by PCR amplification using Phusion® High-Fidelity DNA Polymerase (NEB) from Col-0 genomic DNA/cDNA ...
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bioRxiv - Microbiology 2022Quote: ... Purified PCR products were sequentially digested with the appropriate restriction endonuclease (New England Biolabs) and re-purified ...
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bioRxiv - Neuroscience 2023Quote: ... The full-length PCR product was purified using Monarch Gel Extraction Kit (NEB #T1020), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... all PCRs were performed using Q5 High Fidelity DNA polymerase (M0419; New England Biolabs). A plasmid containing human APOE3-TurboGFP was purchased from Origene (Cat# RG200395) ...
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bioRxiv - Immunology 2023Quote: ... the scFv insert from isolated plasmids was amplified by PCR using Q5 polymerase (NEB). DNA samples were prepped and run using the Illumina MiSeq v3 reagent kit following manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2023Quote: PCR amplification was performed using Q5 high fidelity polymerase (New England Biolabs, Hitchin, UK) and PCR screening was performed using GoTaq Flexi DNA polymerase (Promega ...
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bioRxiv - Microbiology 2023Quote: CloneAmp HiFi PCR Premix (TakaraBio) and Phusion High-Fidelity DNA polymerase (New England BioLabs) were used for PCR reactions for all plasmid constructions ...
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bioRxiv - Genomics 2022Quote: ... Sequencing adapters were then added with two rounds of PCR with Q5 (NEB #M0492) (GWLP P4-7) ...
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bioRxiv - Microbiology 2023Quote: ... The product was purified with a Monarch PCR and DNA cleanup kit (NEB, T1030) and restricted with enzymes Nde1 (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... The enrichment PCR master mixture contained 2,200 OneTaq 2X Master Mix (New England Biolabs), 16.5 uL synHEK3 P7 enrichment primer (100 uM) ...
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bioRxiv - Genomics 2023Quote: ... The in/out PCRs were carried out with Q5 polymerase (New England BioLabs, M0494S), while the out/out PCRs were carried out with PrimeSTAR GXL DNA Polymerase (Takara Bio ...
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bioRxiv - Microbiology 2023Quote: ... PCR fragments were gel-purified and incubated with HiFi DNA assembly enzyme mix (NEB) and transfected into E ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was PCR-amplified using Q5 High-Fidelity 2x Master Mix (NEB M0492) for 20 cycles ...
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bioRxiv - Biophysics 2023Quote: ... purified cDNA was used as a template for PCR using Q5 DNA Polymerase (NEB) and forward and reverse sequencing primers (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using Phusion 5X Master mix (New England Biolabs GmbH, M0492S). For a 25 µl reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using the Monarch PCR and DNA Clean Up Kit (#T1030L, NEB), Illumina NGS libraries were prepared using the NEBNext Ultra II DNA Kit (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using the Monarch PCR and DNA Clean Up Kit (#T1030L, NEB) and ChIP-seq libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7546S ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCR amplifications for plasmid construction were performed using Q5 High-Fidelity Polymerase (NEB). All plasmids were sequence verified through Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... in vitro transcription templates were generated by PCR using Q5 polymerase (New England Biolabs) and primers listed in (Extended Data Table 3 ...
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bioRxiv - Physiology 2023Quote: ... All PCR was achieved using Hot Start Taq DNA Polymerase (New England BioLabs, Inc) under the following settings ...
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bioRxiv - Neuroscience 2023Quote: ... Cloning PCRs were performed using Phusion High Fidelity DNA polymerase (M0530L, New England BioLabs) and then validated by Sanger sequencing ...
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bioRxiv - Immunology 2023Quote: ... tagmented DNA was amplified using NEBNext High-Fidelity PCR Master Mix (New England BioLabs) with the following primers ...
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bioRxiv - Microbiology 2023Quote: ... used for Polymerase Chain Reaction (PCR) and Golden gate cloning kit (New England Biolabs) were carried out according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCRs were performed with Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... the PCR products were then inserted into the opened vector with NEBuilder (NEB, #E5520). Six copies of the MLANA-specific gRNA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs catalog # M0531), and/or LongAmp® Hot Start Taq DNA Polymerase with Expand HF Buffer (Roche catalog # 05917131103 ...
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bioRxiv - Genomics 2023Quote: ... Libraries were amplified using NEBNext HiFi 2X PCR Master mix (NEB cat. no. M0541) with 13 rounds of amplification as described previously28 ...
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bioRxiv - Genomics 2022Quote: ... 25 μl of NEBNext High-Fidelity 2x PCR Master Mix (New England Biolabs, M0541S), 2.5 μL each of Nextera i5 and i7 indexed amplification primers (Nextera Index Kit ...
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bioRxiv - Genetics 2022Quote: ... blunt-end PCR products were generated using Phusion High Fidelity DNA Polymerase (NEB M0530S) and forward primers were designed including 5’-CACC-3’ on the 5’ end for directional cloning and then ligated into MultiSite Gateway™-compatible pME entry vectors using pENTR/D-TOPO (Invitrogen K240020) ...
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bioRxiv - Genetics 2023Quote: ... 5% DMSO and 1× NEBNext High-Fidelity PCR master mix (New England BioLabs, M0541L) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The desired inserts were amplified by PCR using Q5 high-fidelity DNA polymerase (NEB) with the high-GC buffer as per the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2023Quote: ... and for colony PCRs the OneTaq Quick-Load Master Mix Polymerase was used (NEB). PCR products were analyzed on 1 % TAE agarose gels ...
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bioRxiv - Neuroscience 2023Quote: ... The 385 bp PCR product was digested with 1 unit of Hpy188III (NEB #R0622) for 2 hrs at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... the first round of PCR was performed using LongAmp Taq 2X master mix (NEB) and 2 – 3 uL of cDNA (1:5 diluted in water ...
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bioRxiv - Genetics 2023Quote: ... qRT-PCR was performed using the Luna® Universal qPCR Master Mix (NEB, USA) in a 10 μL total sample volume (5 μL 2× Luna® Universal qPCR Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... qRT-PCR was performed using Luna Universal qPCR master mix (SYBR green; NEB, USA) on the QuantStudio™ 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... All PCR fragments were generated using Phusion High-Fidelity DNA polymerase (New England Biolabs). The recombinant plasmids were assembled by T4 ligase or Gibson assembly with enzymes/reagents from New England Biolabs (35) ...
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bioRxiv - Plant Biology 2023Quote: ... The methylated template plasmids remaining in the PCR products were digested by DpnI (NEB), and after transformation to E ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PCR amplification using Q5 High-Fidelity DNA polymerase (New England Biolabs, #M0491) and Sanger sequencing (Macrogen Europe) ...
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bioRxiv - Immunology 2022Quote: ... cleaned and concentrated 20x using the Monarch PCR & DNA Cleanup Kit (5 µg) (NEB). Further cleanup was done with the E-gel imager system (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... Cloning fragments were PCR amplified using Q5 polymerase 2X master mix (New England Biolabs). Full construct sequencing was performed by Plasmidsaurus (Eugene ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... Libraries were amplified by 13 cycles of PCR with Phusion polymerase (New England Biolabs). A size selection step was done using Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Bioengineering 2023Quote: ... After a 30 cycles PCR reaction with Q5 hot-start high-fidelity polymerase (NEB) following recommended vendor protocol ...
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bioRxiv - Cell Biology 2023Quote: ... All missense mutations in PC1 were introduced using PCR amplification and Gibson assembly (NEB) using several unique restriction sites in PC1 ...