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Citations for New England Biolabs :
4601 - 4650 of 7434 citations for rno mir 129 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... desalted using GenepHlow PCR Cleanup Kit (GeneAid) and ligated with T4 DNA ligase (NEB), each step according to manufacturer’s directions ...
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bioRxiv - Systems Biology 2024Quote: ... DNA fragments were amplified by PCR using Q5 DNA polymerase (NEB M0491, for cloning) or OneTaq DNA Polymerase (NEB M0480 ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR amplification of the library region was performed with Q5 High Fidelity Polymerase (NEB) using primers containing Illumina partial adapters ...
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bioRxiv - Synthetic Biology 2024Quote: ... the backbone PCR reaction was digested overnight with DpnI (New England Biolabs, Ipswich, MA) at 37°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1ng of RNA was added to Luna Universal One-Step qRT-PCR mix (NEB) containing 4μmol of each primer on ice ...
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bioRxiv - Biochemistry 2024Quote: ... was used for high-fidelity PCR and the 2X HiFi Gibson Assembly Mix (NEB) was used for amplicon assembly ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Microbiology 2023Quote: ... The variable sgRNA sequences were amplified by nested PCR (Q5 High-Fidelity Polymerase, NEB), the first round of PCR amplifying all sgRNA insertions ...
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bioRxiv - Molecular Biology 2023Quote: SPPiDDR1 flanking sequences were amplified by PCR with a high-fidelity Q5 polymerase (NEB) and primers combining recombination attB sites and the extremity of sequence ...
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bioRxiv - Molecular Biology 2023Quote: ... Polymerase chain reaction (PCR) was performed with Q5-HF polymerase (New England Biolabs, USA) or with CloneAmp Hifi polymerase (Takara Bio ...
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bioRxiv - Molecular Biology 2023Quote: ... and the cDNA libraries were purified using the Monarch PCR & DNA Cleanup Kit (NEB). High-throughput sequencing was performed on an Illumina NextSeq 550 in paired-end mode with 150 cycles per end.
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bioRxiv - Cancer Biology 2023Quote: ... sgRNA inserts were amplified with NEBNext High-Fidelity 2X PCR Master Mix (NEB M0541L). Samples were then pooled in equimolar concentrations ...
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Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)bioRxiv - Biochemistry 2023Quote: ... and 0.5 µL Phusion High Fidelity DNA polymerase in 1x PCR buffer (NEB #B0518S) in a total volume of 50 μL.
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bioRxiv - Biochemistry 2023Quote: ... we performed two-step PCR-mediated site-directed mutagenesis using Phusion HF (NEB, M0530) and primers containing the mutation of interest to generate mutant BRCA1 fragments flanked by attL sequences for LR clonase reaction (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... and regularly tested for mycoplasma contamination (PCR-based assay from Minerva-Biolabs, Berlin, Germany).
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bioRxiv - Immunology 2023Quote: ... CD4 was amplified by PCR using Q5 DNA Hot Start Polymerase (New England BioLabs) in 0.2μM dNTP ...
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bioRxiv - Biophysics 2023Quote: ... DNA fragments were amplified through PCR using Q5® High-Fidelity DNA Polymerase (NEB) following manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... All missense mutations in PC1 were introduced using PCR amplification and Gibson assembly (NEB) using several unique restriction sites in PC1 ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons were obtained using the Q5 High-Fidelity Master Mix (New England, BioLabs), 0.4 μM each primer ...
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bioRxiv - Synthetic Biology 2023Quote: Polymerase Chain Reaction (PCR) was performed using Q5 High Fidelity 2X Master Mix (NEB) with primers synthesized by Integrated DNA Technologies (IDT ...
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bioRxiv - Biochemistry 2024Quote: ... Correct assembly was verified by PCR using Taq 2X Master Mix (New England BioLabs) and Sanger sequencing (Genewiz).
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bioRxiv - Microbiology 2023Quote: ... PCR fragments and linearized pDIA6754 were mixed and assembled using Gibson Assembly (NEB, France) and transformed by heat shock in E ...
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bioRxiv - Cell Biology 2023Quote: ... All PCR reactions were performed using the Phusion high fidelity DNA polymerase (NEB, M0530S). All the PCR-amplified fusion genes and epitope tags were cloned into the pSKP32 vector (Kaeser-Pebernard et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... ∞ at 4 °C) using NEBNext HighFidelty 2x PCR Master Mix (New England Biolabs, M0541S) and Illumina i5 and i7 indices54 ...
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bioRxiv - Microbiology 2024Quote: ... PCR products and vector pBRCS12 were digested with HindIII and BamHI (New England Biolabs) restriction endonucleases ...
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bioRxiv - Molecular Biology 2023Quote: ... and then purified with a Monarch PCR & DNA Cleanup kit (New England Biolabs, USA). For IVT reactions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Recovery products were then purified with the Monarch® PCR & DNA Cleanup Kit (NEB) and eluted in 10 µl of the Monarch® DNA Elution Buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was done using the Luna Universal qPCR Master Mix (New England Biolabs) with WLV primers 4361+4264 and as the reference gene spinach Actin 11 (primers 4614+4615 ...
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bioRxiv - Genetics 2024Quote: ... and the exogenous THAP1 sequences in each sample amplified by PCR (Q5, NEB #M0493L), using primers annealing to invariant regions flanking each mutagenized segment ...
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bioRxiv - Genomics 2024Quote: ... The assembly was then purified with the Monarch PCR & DNA cleanup kit (NEB, T1030S), eluted in 6 µL of water ...
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bioRxiv - Synthetic Biology 2024Quote: ... TCSs were amplified by PCR using Q5 High-Fidelity DNA polymerase (New England Biolabs), and amplified PCR fragments were purified by column purification using the Monarch PCR Cleanup Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... PCR verification was performed using OneTaq 2X Master Mix with Standard Buffer (NEB #M0482) or LongAmp Hot Start Taq 2X Master Mix (NEB #M0533).
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bioRxiv - Cell Biology 2024Quote: ... PCR verification was performed using OneTaq 2X Master Mix with Standard Buffer (NEB #M0482).
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bioRxiv - Microbiology 2024Quote: ... Amplicons were purified using the Monarch DNA and PCR Cleanup Kit (New England Biolabs) and precipitated as per the Co-Precipitant Pink (Bioline ...
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bioRxiv - Genomics 2024Quote: ... Templates for sgRNAs were generated by PCR with Phusion High-Fidelity DNA Polymerase (NEB), purified using the QIAquick PCR purification kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2024Quote: ... purified using column DNA Clean-up kit (Monarch PCR & DNA Cleanup Kit, # T1030L, NEB) and mixed with Nhe1 and BamH1 double-digested and gel-purified target pCW57.1 vector in a molecular ratio of 1:2 ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was used as a template for PCR (Phusion HF kit (New England Biolabs)) with primers designed to amplify the spike and HE genes (Table 2) ...
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bioRxiv - Biophysics 2024Quote: ... PCR was performed using Q5 HotStart high fidelity master mix (New England Biolabs #M0494S). Following KLD enzyme treatment (NEB #M0554S) ...
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bioRxiv - Bioengineering 2024Quote: Polymerase chain reaction (PCR) of constructs utilized for plasmid assembly were amplified by NEB Q5 Hot-Start 2X Mastermix according to the manufacturer’s protocol (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... PCR amplification was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) for 35 cycles with a 20-second extension time ...
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bioRxiv - Cell Biology 2024Quote: ... ▴CRITICAL For PCR amplification we use the Q5® High-Fidelity DNA Polymerase (NEB) but other can be picked such as Phusion ...
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bioRxiv - Cell Biology 2024Quote: ... Both PCR and qPCR were performed using Pfusion High-Fidelity Polymerase Master Mix (NEB) and full-length Solexa P3/5 primers ...
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bioRxiv - Genetics 2024Quote: ... and SV40 polyA PCR products were assembled in pattB-w-using Gibson assembly (NEB). Act5C-sfGFP and 3xP3-sfGFP integrations into attP40 were recovered by BestGene ...
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bioRxiv - Developmental Biology 2024Quote: ... The libraries were amplified using NEB Next High- Fidelity 2X PCR Master Mix (NEB) with primer extension at 72°C for 5 min ...
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bioRxiv - Systems Biology 2024Quote: ... These PCR products were used for Gibson assembly with the barcoded promoters (#E2611S; NEB). Gibson assembly reactions were purified with magnetic beads and 2 µl of the reaction were electroporated into 20 µl of electrocompetent e ...
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bioRxiv - Biophysics 2024Quote: ... the mixture was purified using the Monarch PCR and plasmid DNA purification kit (NEB), eluted in 10 mM Tris-HCl pH 8.0 and stored at -20 °C for further experiments ...
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bioRxiv - Biochemistry 2024Quote: ... the ecpga gene (GenBank: X04114.1) was amplified by PCR using Q5 polymerase (NEB, USA) from our previous plasmid pKA1868 with ecPGA cloning primers bearing NdeI and XhoI site overlaps ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5μl of the PCR product was mixed with 6x loading buffer (New England BioLabs) and electrophoresed on a 1.5% TAE agarose gel for F1534C and V410L ...
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bioRxiv - Cancer Biology 2024Quote: ... transposed DNA was mixed with NEBNext high-Fidelity 2x PCR Master Mix (NEB, #M0541S), AD1_noMX and AD2.1-2.16 barcoded primers ...
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bioRxiv - Molecular Biology 2024Quote: The point and deletion mutants of SMG8 were PCR amplified using Q5 polymerase (NEB) and inserted with an N-terminal FLAG-tag via NheI and NotI restriction sites into the tetracycline-inducible pcDNA5/FRT/TO vector (Thermo Fisher Scientific) ...