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Citations for New England Biolabs :
201 - 250 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... In vitro transcription reactions were performed using a Standard RNA Synthesis Kit (NEB). 1 µg of purified RNA was subjected to reverse transcription using a Transcriptor First Strand cDNA Synthesis Kit (Roche ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1x of OneTaq Hot Start 2X Master Mix with Standard Buffer (NEB, M0484L), and 1x Chai Green Dye (CHAI ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1x of OneTaq Hot Start 2X Master Mix with Standard Buffer (NEB, M0484L). The qPCR was performed on CFX96 Touch Real-Time PCR Detection System (BioRad) ...
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bioRxiv - Synthetic Biology 2023Quote: ... with standard restriction enzymes (New England BioLabs; HF enzymes were used when possible) and ligation with T4 DNA ligase (New England BioLabs) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were constructed by standard restriction ligation methods using T4 DNA ligase (NEB) or using the NEBBuilder assembly kit (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... were inserted via standard cloning techniques and digested with NcoI and NotI (NEB) restriction enzymes ...
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bioRxiv - Physiology 2023Quote: ... using the first strand cDNA synthesis standard protocol with random primers (S1330, NEB), 2.5 mM dNTPs (N0446 ...
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bioRxiv - Cell Biology 2023Quote: ... and standard cut-and-paste cloning using T7 DNA ligase (New England Biolabs). NESRev ...
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bioRxiv - Microbiology 2023Quote: ... The protein ladder used was Colour Protein Standard Broad Range (NEB, Ipswich, US).
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bioRxiv - Molecular Biology 2023Quote: ... Line- arized plasmid standards were obtained by digesting pAAV_GFP with HindIII (NEB, R3104S) restriction enzyme.
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bioRxiv - Neuroscience 2024Quote: ... 12.5 µL OneTaq Quick-Load 2X Master Mix with Standard Buffer (Biolabs, M0486S), 6.5 µL Nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... The Color Protein Standard Broad Range (10–250 kDa) (New England BioLabs Inc.) was used as protein molecular weight ladder ...
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bioRxiv - Microbiology 2021Quote: ... Linearized DNA was purified by phenol/chloroform extraction and RNA was in vitro transcribed in the presence of G(5’)ppp(5’)G RNA Cap structure analog using SP6 polymerase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... gRNA modules were combined with pGG-C-linker-G plasmid and cloned into pEN-R2-A-G-L3 by restriction-ligation using BsaI enzyme (New England Biolabs) to obtain pEN-R2-gRNA_BRI1-3-gRNA_BRI1-2-L3 ...
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bioRxiv - Microbiology 2021Quote: ... 9 Neuraminidase A (P0722, NEB, 4 units/µg protein). 10 µg glycoprotein ...
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bioRxiv - Biochemistry 2022Quote: ... 50 µg/mL bovine serum albumin (New England BioLabs)) and incubated for 30 min at room temperature (~22°C) ...
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bioRxiv - Microbiology 2022Quote: ... or 5 µg pTOPO dsRNA plasmids with EcoR1 (NEB) in reaction containing 1X T7 buffer (50mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... and 200 µg/ml proteinase K (New England Biolabs) and incubated for 2 hrs at 55°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 ul ET SSB (500 µg/ml, NEB M2401S) was also added ...
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bioRxiv - Systems Biology 2024Quote: ... 1 µg gDNA was digested with 4U MmeI (NEB) in a 50 µl reaction at 37°C ...
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bioRxiv - Genomics 2024Quote: ... and 100 µg/mL rAlbumin (New England Biolabs B9200S) in 1X PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... 10µl of Protein G magnetic beads (NEB, S1430S) was used ...
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bioRxiv - Cell Biology 2020Quote: ... 10 μL Protein G magnetic beads (NEB, S1430S) were used ...
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bioRxiv - Biochemistry 2024Quote: ... 30 μL of Protein G Magnetic beads (NEB) was mixed with 1 μL of anti-RelA antibody (Diagenode ...
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bioRxiv - Biochemistry 2020Quote: ... excised bands containing 20 μg of gp145 were incubated with 5000 units of PNGase F (NEB) for 24 hrs at 37°C [31] ...
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bioRxiv - Molecular Biology 2020Quote: ... 6μg of sonicated DNA was pretreated with 12 μl of RNase H (5000 U/mL; NEB) for incubation at 37°C for 6h prior to immunoprecipitation by S9.6 antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunoblots in this study were anti-tau (tau46) (1:5000; NEB 4019S), anti-human tau (tau13 ...
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bioRxiv - Developmental Biology 2020Quote: ... pCS2+MT-hoxb4a was linearized with NotI and transcribed with SP6 RNA polymerase in the presence of a G(5′)ppp(5′)G RNA cap structure analog (New England BioLabs Inc.). 25 pg of hoxb4a mRNA was injected into one-cell-stage embryos.
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bioRxiv - Developmental Biology 2021Quote: ... the BAC(zic1:zic1-Myc/zic4:DsRed) was co-injected with I-SceI Meganuclease (NEB) into 1-cell stage Da embryos ...
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bioRxiv - Neuroscience 2022Quote: ... C57BL/6NJ zygotes were co-injected with Cas9 protein (50 ng/μl) (New England Biolabs) or Cas9 mRNA (1000 ng/μl ...
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bioRxiv - Cell Biology 2020Quote: ... via restriction free cloning using NEBuilder (New England Biolabs). All inserts have been sequence verified prior the respective plasmid usage.
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bioRxiv - Cell Biology 2020Quote: ... Transcription reactions were treated with RNase-free DNaseI (NEB) to digest the DNA template at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... DNA templates were removed with RNase-free DNaseI (NEB), and RNAs were poly-adenylated with E ...
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bioRxiv - Microbiology 2020Quote: ... A DNase I RNAse free 2000 U/ml (NEB) treatment was done using 1 μl for 15 minutes at 37°C ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... cDNA samples were diluted in Nuclease-free water (NEB) before use in qPCR reactions ...
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bioRxiv - Developmental Biology 2022Quote: ... and 60 U/ml DNaseI (RNase-free, NEB, M0303L) were added and the organoids were triturated gently with a P1000 pipette with a wide-bore tip ...
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bioRxiv - Molecular Biology 2022Quote: ... treated with RNAse-free DNAse I (New England Biolabs) and purified by phenol/chloroform extraction ...
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bioRxiv - Biochemistry 2020Quote: ... treated with RNase-free DNase I (New England Biolabs), and purified by phenol:chloroform extraction and ethanol precipitation ...
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bioRxiv - Bioengineering 2024Quote: LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA using primers oAS344 for cRNA and oAS345 for vRNA (Supplementary Note 21).
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bioRxiv - Biochemistry 2023Quote: ... 2 µl PNGaseF (P0705L, Glycerol-free, New England Biolabs) was added and incubated for one hour at 57⁰C ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 U/μl of DNase I (RNase free) (NEB) was added to each reaction followed by 30 min incubation at 37°C for the elimination of the DNA templates ...
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bioRxiv - Biochemistry 2024Quote: ... DNA templates were digested using RNase-free DNase (NEB), and RNA was phenol-chloroform extracted followed by isopropanol precipitation (overnight at −80°C) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was treated with RNAse-free DNAse I (NEB) for 10 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Protein molecular ladder (Color Prestained Protein Standard, Broad Range 11–245 kDa, NEB, P7712S). Dry transfer was performed using an iBlot™ 2 gel transfer device (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... OneTaq 2x Master Mix with Standard Buffer was used (New England BioLabs, Ipswich, MA). As a template in the first round ...
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bioRxiv - Immunology 2021Quote: ... OneTaq 2x Master Mix with Standard Buffer was used (New England BioLabs, Ipswich, MA). As a template in the first round ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: ... against a Colour Prestained Protein Standard (Broad Range 10-250 kDa, New England BioLabs) and resolved by electrophoresis for 70 minutes at 150-180V ...
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bioRxiv - Biochemistry 2022Quote: The standard N-glycoprotein bovine pancreatic ribonuclease B (RNase B, New England Biolabs, USA) was used as a substrate to measure NGLY1 activity ...
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bioRxiv - Microbiology 2020Quote: ... Standard protocols for PCR were followed using Q5 polymerase (New England Biolabs, Ipswitch MA). PCR was performed using manfacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: We followed standard protocol of NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Cat.No.E7490) to isolate messenger RNA from the purified total RNA and stored them at −80°C.