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Citations for New England Biolabs :
251 - 300 of 1210 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... plantarum B21 plasmids using OneTaq® 2X Master Mix with Standard Buffer (NEB, USA) in a T100™ Thermal Cycler (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA samples were PCR-amplified using standard Illumina adaptors and Q5 PCR mix (NEB) with 10-13 cycles (SMInputs ...
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bioRxiv - Bioengineering 2023Quote: Plasmids were generated by standard molecular biology techniques including HiFi assembly (New England BioLabs). All plasmids were prepared for microinjection using the NucleoBond Xtra Midiprep kit EF (Machery-Nagel ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein amounts for deadenylation assays were estimated against BSA standards (New England Biolabs, B9000S).
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bioRxiv - Biochemistry 2023Quote: ... and K723G).49 His/MBP-BRAF NTs were created with standard Gibson Assembly (NEB) procedure in the pET28-MBP vector from the following primers ...
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bioRxiv - Biochemistry 2023Quote: Cloning was performed using standard molecular biology techniques to assemble Q5 DNA polymerase (NEB) amplified or synthesized (IDT ...
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bioRxiv - Molecular Biology 2022Quote: ... amplified and indexed by the standard protocol for small RNA sequencing (New England Biolabs). Small DNA libraries were sequenced using the HiSeq2500 platform (Illumina ...
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bioRxiv - Biochemistry 2022Quote: ... A standard PCR protocol with Phusion® High Fidelity DNA polymerase (New England Biolabs) and primer-specific annealing temperatures was used for the DNA amplification ...
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bioRxiv - Neuroscience 2023Quote: ... Plasmids were constructed using standard restriction enzyme cloning or Gibson assembly (New England BioLabs). All plasmids used in this work are described in Supplementary file 2.
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bioRxiv - Molecular Biology 2023Quote: ... 10 µl of a broad range color pre-stained protein standard (New England BioLabs) was added to the outer lanes ...
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bioRxiv - Cell Biology 2024Quote: ... PCR verification was performed using OneTaq 2X Master Mix with Standard Buffer (NEB #M0482) or LongAmp Hot Start Taq 2X Master Mix (NEB #M0533).
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bioRxiv - Cell Biology 2024Quote: ... PCR verification was performed using OneTaq 2X Master Mix with Standard Buffer (NEB #M0482).
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bioRxiv - Biochemistry 2024Quote: ... BRSKs were also cloned into a pcDNA3 vector using a standard T4-ligase (NEB) protocol and expressed in frame with a 3C-protease cleavable N-terminal tandem STREP-tag ...
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bioRxiv - Synthetic Biology 2024Quote: ... Taq reactions were performed in a 1X Taq Standard Buffer (New England Biolabs, B9014) containing 10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: All proteins were purified from standard pET28a vectors expressed in BL21DE3 cells (NEB, C2527H). FoTO1 and FoTO1ΔCterm were purified as C terminal fusions ...
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bioRxiv - Cell Biology 2020Quote: ... 1.0 µg of total RNA was DNAseI treated (NEB #T2010) and retro-transcribed by using 100 units of M-MLV Reverse transcriptase (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Ten µg of RNA was treated with 2U DNase (NEB) at 37°C for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 µg of DNA was digested with DpnI (NEB; R0176) in 10 µl volume at 37°C for 16h ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µg/ml bovine serum albumin (BSA, New England Biolabs), and DNA substrate (1 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg of replicon plasmid was linearised with AscI (NEB), purified by phenol-chloroform extraction ...
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bioRxiv - Genomics 2024Quote: ... 10 µg of DNA were digested with BamHI (60U, NEB) overnight ...
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bioRxiv - Genetics 2024Quote: ... Reactions were then treated with 40 µg Monarch RNaseA (NEB) for 1 hour and then treated with 1.6 units of Proteinase K (NEB ...
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bioRxiv - Biophysics 2022Quote: ... 1 µL of 0.4 µg µL-1 Lys-C (NEB) stock was added (enzyme:substrate ratio of 1:100 w/w ...
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bioRxiv - Microbiology 2022Quote: ... (2 µg enzyme in 100 µL H2O) (New England Biolabs) at 37 °C overnight ...
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bioRxiv - Genomics 2024Quote: ... 1 µg of total RNA was treated with DNaseI (NEB) and purified with Agencourt® RNAClean™ XP (Beckman Coulter) ...
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bioRxiv - Biophysics 2024Quote: ... and 0.15 − 0.2U/µg EcoRV-HF (New England Biolabs, R3195) to digest the backbone into 200-300 bp fragments for two to three overnights ...
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bioRxiv - Biochemistry 2024Quote: ... which was dephosphorylated at the 5’-end with 3 µl of Quick-CIP (5000 U/µl, NEB) in a total volume of 20 µl according to manufactureŕs instructions ...
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bioRxiv - Genomics 2020Quote: ... The restriction enzymes SfaN1 (NEB; cuts specifically rs4702 G) and Bsr1 (NEB ...
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bioRxiv - Immunology 2020Quote: ... pCMV-VSV-G was cut by BamHI-HF (NEB) for removing the VSV-G coding region and the digested PCR products were cloned into this site ...
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bioRxiv - Neuroscience 2024Quote: ... 25-50 ul Protein G Magnetic Beads (NEB #S1430S) were incubated with 3ug GFP (D5.1 ...
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bioRxiv - Microbiology 2022Quote: ... 30 μg of Protein G magnetic beads (NEB #S1430) was washed with 250 μl of Immunoprecipitation Reaction Buffer (150 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... then capped with m7G (5’) ppp (5’) G (NEB) and tailed with a poly(A ...
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bioRxiv - Developmental Biology 2024Quote: ... 10 mM dNTP mix (dC/G/TTP, NEB, N0446) and 0.8ml of 5U/µl DNA Polymerase I ...
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bioRxiv - Synthetic Biology 2020Quote: ... Antibodies were then co-incubated with a 20-fold molar excess of BG-GLA-NHS (NEB) for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... The resulted PCR product co-transformed with pEGFP-C1-2µ-URA3 cut with BamHI (NEB, R3136) into yeast strain BY4743 (EUROSCARF Y20000 ...
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bioRxiv - Molecular Biology 2024Quote: ... and constructs without a Csy4 recognition site were co-transfected with 750ng pUC19 (New England Biolabs) to balance total transfected mass ...
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bioRxiv - Genetics 2021Quote: ... Then each of the 10 constructs were transformed into standard DH5α competent bacteria (#C2987; NEB) grown overnight in in 500 ml of standard Luria Broth(LB ...
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bioRxiv - Genomics 2020Quote: ... For RNA synthesis we used the NEB Standard RNA Synthesis protocol (E2050, New England Biolabs). We then added 1.5x volumes of ethanol to the reaction ...
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bioRxiv - Immunology 2022Quote: ... Realtime PCR is conducted following manufacturer standard protocol (Luna Universal qPCR Master Mix, NEB Inc). Sybr green RT-PCR primers used in this study is listed in Table 1.
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bioRxiv - Synthetic Biology 2020Quote: ... which were then assembled using a standard Golden Gate assembly method (New England Biolabs, E1601S) to create a full-length linear insert ...
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bioRxiv - Genetics 2021Quote: ... Each reaction consisted of 2.5 µL 10X Standard Taq Reaction Buffer (NEB, Ipswich, MA, USA), 0.5 µL 10 mM dNTPs (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... All constructs were generated by standard cloning or by Gibson Assembly (NEBuilder HiFi Assembly, NEB) using XL10-Gold bacteria (Agilent) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...
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bioRxiv - Bioengineering 2021Quote: ... EP-PCR was performed using Taq polymerase and the standard Taq buffer (New England Biolabs) with imbalanced dNTP (0.2 mM dATP ...
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bioRxiv - Cell Biology 2020Quote: For cloning standard restriction enzyme digests or oligo annealing and ligations using enzymes from NEB was used ...
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bioRxiv - Genomics 2022Quote: ... 30 cycles of endpoint PCR were performed using NEB Standard Taq (New England Biolabs, USA). PCR products (6 μL ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli using standard transformation techniques with NEB 5-Alpha chemically competent cells (New England BioLabs), and to C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and Q5 High-Fidelity DNA polymerase in a standard buffer (New England Biolabs, Ipswich, USA). Amplicons were purified on MinElute PCR Purification columns (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... to visualize the bands alongside a color prestained protein broad range standard (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: All constructs were generated by standard cloning or by Gibson Assembly (NEBuilder HiFi Assembly, NEB) using XL10-Gold bacteria (Agilent) ...