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Citations for New England Biolabs :
4501 - 4550 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Beads were resuspended in a 100 μL blunt enzyme mix reaction (Quick Blunting Kit, NEB) with half the enzyme concentration of the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... 72°C for 60 seconds) in 50 µl reactions using Phusion PCR polymerase mix (NEB) containing 25 pmol each of the following ultramers (the protospacer-matching sequence is underlined) ...
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bioRxiv - Bioengineering 2019Quote: ... LAMP mix contained 10 µL 2×WarmStart LAMP Mastermix (New England Biolabs, Ipswich, MA, USA) and 6 µL water ...
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bioRxiv - Biophysics 2020Quote: ... Digestion mix (84 μl water, 10 μl 10x NEB Buffer 3.1, 6 μl Nt.BspQ1 (NEB)) was prepared and heated to 50 oC for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... Captured fragments were subjected to end repair with End Repair Enzyme Mix (New England Biolabs) and dA tailing with Klenow Fragment exo 5U/uL (New England Biolab) ...
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bioRxiv - Cell Biology 2022Quote: SLC25A46 was amplified through PCR with Taq-polymerase with 7-deaza GTP/nucleotide mix (NEB) using cDNA from control fibroblasts as a template and cloned into Gateway modified pBABE-Puro using Gateway Cloning Technology (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: Purified RNA was digested and dephosphorylated to single nucleosides using nucleoside digestion mix (NEB, M0649S) at 37 °C for 2 hours ...
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bioRxiv - Plant Biology 2024Quote: ... Backbones and fragments were assembled with NEBuilder® HiFi DNA assembly mix (New England Biolabs) / In-fusion Master mix (Takara ...
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bioRxiv - Neuroscience 2024Quote: ... An imaging activation mix was prepared by adding RNAse inhibitor (100 μL, New England BioLabs) to 250 μL Imaging Buffer Activator (VIZGEN ...
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bioRxiv - Microbiology 2024Quote: ... Deletions of single genes in plasmid pGM255 were performed using the KLD enzyme mix (NEB).
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bioRxiv - Evolutionary Biology 2024Quote: ... Three micrograms of DNA were repaired using the NEBNext FFPE DNA Repair Mix (NEB M6630). End repair and dA-tailing were performed using the NEBNext End repair/dA-tailing Module (E7546 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The reaction mix was purified with Monarch® PCR & DNA Cleanup Kit (NEB, Ipswich, MA). Next ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR mix was prepared for using the Phusion® High-Fidelity DNA Polymerase (NEB) and primer sequences are listed in Table S3 ...
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutagenesis was performed using Phusion DNA polymerase PCR mix (New England Biolabs, UK). Primers were designed specifically to replace the nucleotides coding for serine at the 44th position in the CDS of OsbZIP1.1 with those for alanine as given in table S1.
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bioRxiv - Molecular Biology 2022Quote: ... single cells were lysed in 100 nL lysis mix (10 nL 1X Cutsmart buffer (NEB), 10 nL Proteinase K (Ambion) ...
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bioRxiv - Molecular Biology 2022Quote: ... Purified RNA was digested and dephosphorylated to single nucleosides using Nucleoside Digestion Mix (NEB, M0649S) at 37 °C for 1 h ...
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bioRxiv - Biophysics 2023Quote: The Q209L mutation was generated in the same construct using the KLD enzyme mix (NEB). Expression and purification of Gαq Q209L were the same as for the wildtype protein with the following modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer ...
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bioRxiv - Microbiology 2023Quote: ... Molecular cloning was performed using the Gibson assembly method with HiFi assembly enzyme mix (NEB) (64) ...
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bioRxiv - Bioengineering 2023Quote: ... DNA was fragmented for 20 minutes using the Ultra II FS Enzyme Mix from NEB. 100uM Splinkerette V1.2TS and V1.2BS oligos from IDT were annealed in an Eppendorf tube by heating the mixture to 98 C for 10 min in a heat block and then unplugging the heat block to allow the reaction to cool to room temperature ...
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bioRxiv - Biophysics 2023Quote: ... KODextreme polymerase was used for amplification and the product was ligated (KLD enzyme mix, NEB) and transformed into NEB® 5-alpha Competent cells ...
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bioRxiv - Microbiology 2023Quote: ... 2.5uL from a single native barcode and 25 µL Blunt/TA Ligase Mix (NEB, M0367S) were added ...
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bioRxiv - Genomics 2023Quote: Cells were resuspended in 330 μl of ligation mix (1x T4 ligase buffer (NEB, B0202S), 100 Units T4 DNA ligase (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... followed by 23.5 μl of ligation mix (3 μl 1x T4 ligase buffer (NEB, B0202S), 0.15 μl 50 mg/ml BSA (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... beads were resuspended in 30 µL RNA ligation mix (1X RNA ligase I buffer (NEB), 1 µL RNA ligase I (NEB) ...
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bioRxiv - Genetics 2023Quote: pASHH3::3xFLAG-ASHH3-HA was constructed using NEBuilder HiFi DNA assembly Mix (New England Biolabs) and pPLV01 vector41 ...
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bioRxiv - Genomics 2024Quote: ... DNA was digested into single nucleosides by using the Nucleoside Digestion Mix (New England Biolabs) enzyme cocktail at 37°C for 2 h ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 1-2 µg phi47 DNA was treated with Nucleoside Digestion Mix (New England Biolabs) following the manufacturer’s protocol at 37°C for >2h ...
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bioRxiv - Genomics 2024Quote: ... and Template Switching RT Enzyme Mix protocol from NEB (New Engand Biolabs, Cat. No. #M0466L). cDNA was PCR amplified using the NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Obtained cDNA template was amplified by PCR using the LongAmp® Taq 2XMaster Mix (NEB) for sequencing.
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bioRxiv - Plant Biology 2024Quote: ... This fragment was inserted by assembly reaction (NEBuilder HiFi DNA Assembly Mix, New England Biolabs) between the CaMV 35S promoter and HDV ribozyme sequences of the small ...
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bioRxiv - Synthetic Biology 2021Quote: ... before gel extraction (0.8 % agarose gel, gel green dye, 80V, 100 minutes; T1020S, New England Biolabs Monarch). Plasmid backbone (50 fmol ...
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bioRxiv - Microbiology 2021Quote: ... Official CDC SARS-CoV-2 N1 gene primers and TaqMan probe set were used [58] with the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs):
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bioRxiv - Microbiology 2020Quote: ... Final Illumina libraries were assessed for quality using an Agilent Bioanalyzer DNA High Sensitivity Assay and qPCR quantification was performed using NEBNext Library Quant kit for Illumina (New England Biolabs). Individual libraries were pooled equimolarly ...
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bioRxiv - Molecular Biology 2021Quote: ... The mRNA abundance levels were determined using 200 ng total RNA for each sample in technical replicates using Luna Universal One-Step RT-qPCR kit (New England BioLabs) and SYBR Green as detection agent and gene-specific primers (Table S2) ...
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bioRxiv - Microbiology 2019Quote: ... using Agilent High Sensitivity DNA kit in a Bioanalyzer 2100 instrument then quantitated using qPCR based NEBNext Library Quant Kit for Illumina (New England Biolabs) with a Piko-Real Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... samples were diluted to 5ng/uL and used for qRT-PCR with Luna One-Step Universal qPCR kit (NEB E3005). Gene specific primers for target transcripts were used at a final concentration of 400uM with 15ng of RNA ...
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bioRxiv - Microbiology 2019Quote: ... Library quality was assessed using an Agilent Tapestation 4200 instrument and quantity determined by qPCR using an NEBnext library quantification kit (NEB).
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bioRxiv - Pathology 2021Quote: ... β-ENaC/SCNN1B (Hs00165722_m1) and γ-ENaC/SCNN1G (Hs00168918_m1) were performed with the Luna Universal Probe One-Step RT-qPCR Kit (NEB, E3006L). Expression of each gene was normalized to 18S (Hs99999901_s1) ...
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bioRxiv - Bioengineering 2020Quote: ... We performed assays for the E and RNase P genes separately in 20 μL reaction volumes using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs). The final concentrations of primer and probe were 400 and 200 nM ...
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (EV Table 1 ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... PCR was carried out in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 N-specific primers (Forward 5′-TAA TCA GAC AAG GAA CTG ATT A-3′ ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... 5′-TAATCAGACAAGGAACTGATTA-3′ (Forward) and 5′-CGAAGGTGTGACTTCCATG-3′ (Reverse) were used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in an Applied Biosystems QuantStudio 6 thermocycler or an Applied Biosystems StepOnePlus system ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... were amplified in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler ...
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bioRxiv - Biochemistry 2021Quote: ... 5’-TAATCAGACAAGGAACTGATTA-3’ (Forward) and 5’-CGAAGGTGTGACTTCCATG-3’ (Reverse) were used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in an Applied Biosystems QuantStudio 6 thermocycler ...
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bioRxiv - Bioengineering 2021Quote: ... RNA was purified using the Macherey Nagel RNA extraction kit following manufacturer’s instruction and viral RNA uptake was quantified using the Luna universal One-Step RT-qPCR kit (NEB; E3005).
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 RNA levels were quantified with 250 ng of RNA inputs using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), using real-time RT-PCR primer/probe sets 2019-nCoV_N1 (CDCN1 ...
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bioRxiv - Microbiology 2021Quote: ... PCR was carried out in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 NP-specific primers (Forward 5’-TAA TCA GAC AAG GAA CTG ATT A-3’ ...
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bioRxiv - Plant Biology 2021Quote: ... For real-time qPCR experiments oligo(dT)-primed cDNAs were synthesized using 2ug of total RNA with MMLV (reverse transcriptase, NEB). qRT-PCR reactions were set up with 50-70ng of cDNA ...
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bioRxiv - Microbiology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...