Labshake search
Citations for New England Biolabs :
4551 - 4600 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The Luna Universal One-Step RT-qPCR kit (E3005L) was used for qRT-PCR analysis following the protocol described by the supplier (NEB) by using a CFX96 real-time C1000 thermal cycler (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Library quality was assessed using an Agilent Tapestation 4200 instrument and quantity determined by qPCR using an NEBnext library quantification kit (NEB). Libraries were sequenced as described previously (43 ...
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bioRxiv - Microbiology 2021Quote: ... RNA levels of target proteins were subsequently quantified by using RT-with the Luna Universal One-Step RT-qPCR Kit (NEB) in an Applied Biosystems QuantStudio 7 thermocycler using gene-specific primers ...
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bioRxiv - Microbiology 2022Quote: The copy number of viral RNAs were titrated by qRT-PCR using Luna Universal Probe One-Step RT-qPCR Kit (NEB) and LightCycler 96 instrument (Roche) ...
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bioRxiv - Genomics 2022Quote: Real-time PCR (RT-PCR) amplification of RNA was performed following the Luna Universal One-Step RT-qPCR kit (New England Biolabs). A CFX 96 Touch Real-Time PCR Thermocycler/ Detection System (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... and nCoV_IP4-14084Probe(+) TCATACAAACCACGCCAGG [5’]Hex [3’]BHQ-1 19) and the Luna Universal Probe One-Step RT-qPCR Kit (NEB). Serial dilutions of a titrated viral stock were analyzed simultaneously to express viral loads as eqPFU per gram of tissue.
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bioRxiv - Molecular Biology 2019Quote: ... quantified by qPCR using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs® Inc., MA, USA), and fragment size (330 bp ...
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bioRxiv - Microbiology 2021Quote: ... then 10 μL of diluted cDNA was used with the NEB Luna Universal Probe qPCR Mastermix (New England BioLabs®) following the manufacturer’s protocol and RT-qPCR performed in 384-well plates using a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems™) ...
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bioRxiv - Microbiology 2019Quote: ... whereas KAPA HyperPlus libraries and libraries sequenced on the Ion Torrent PGM platform were quantified by qPCR using the NEBNext Library Quant Kit for Illumina (New England BioLabs) or the KAPA Library Quantification Kit for Ion Torrent platforms (Figure 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were purified with a 0.8x bead to library ratio and eluted in 30μL EB buffer and qPCR quantified using the NEBNext Library Quant Kit for Illumina (New England BioLabs Inc.). Purified libraries were pooled equimolarly according to the qPCR results and sequenced at the GeoGenetics Sequencing Core ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR reactions were carried out using LightCycler 96 instrument and following reagents: Luna Universal One-Step RT-qPCR Kit (New England Biolabs, NEB) for hCoV-229E and hCoV-OC43 ...
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bioRxiv - Microbiology 2021Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) and a CFX96 Real-Time System ...
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bioRxiv - Pathology 2021Quote: Reverse-transcription polymerase chain reaction (RT-PCR) was performed using the Luna Universal Probe One-Step RT-qPCR kit (NEB). Two gene targets were used for SARS-CoV-2 RNA detection ...
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bioRxiv - Microbiology 2020Quote: ... separate reactions were performed for the quantification of SARS-CoV-2 N and E gene transcripts as well as cellular RNaseP for normalization using the Luna Universal Probe One-Step RT-qPCR Kit (NEB) on a Bio-Rad CFX384 Touch system ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed from 1µL of template RNA in a final volume of 5 μL per reaction in 384-well plates using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a QuantStudio 6 Flex thermocycler (Applied Biosystems) ...
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bioRxiv - Zoology 2023Quote: ... Library concentration and quality were assessed with the Qubit Fluorometer and Bioanalyzer and molarity was estimated via qPCR with the NEBNext Library Quant Kit (New England Biolabs). Libraries were single indexed with NEBNext Multiplex Oligos (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... the concentration was measured by qPCR using a NEBNext Library Quant Kit for Illumina (New England BioLabs GmbH, Hessen, Germany), and the size was determined by Bioanalyzer 2100 using Agilent High Sensitivity DNA Kit (Agilent Technologies GmbH ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: Quantification of SARS-CoV-2 E gene subgenomic mRNA (sgmRNA) was conducted using Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) on a Step One Plus Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... 10 μM SARS-CoV-2 E-gene Forward (5’- ACAGGTACCTTAATAGTTAATAGCGT-3’) and Reverse (5’-ATATTGCAGCAGTACGCACACA-3’) primers were used with Luna Universal One-step RT-qPCR kit (New England Biolabs) and added to 1 μl supernatant (5μl total ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription and quantitative real-time PCR was performed using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in a 96-well plate and qPCR was performed in a LightCycler 96 System (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was diluted 1:10 and 3 µl of diluted cDNA was used as input for qPCR using Luna by NEB master mix for a 20 µL total reaction ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR for SARS-CoV-2 from cell lysates was performed using the TaqMan assay for the CDC N1 gene primers and probes from Integrated DNA Technologies (catalog no. 10006600; Integrated DNA Technologies) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) as previously described (6) ...
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bioRxiv - Microbiology 2022Quote: ... RNA samples were then subjected to qRT-PCR of BB0838 and flaB transcripts using the Luna Universal One-Step RT-qPCR kit (NEB) and an ABI Prism 7500 system (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs) with the primers listed in Table S4 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL lysate was used as a template for the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) on a Light Cycler 480 (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... Library quality was assessed using an Agilent Tapestation 4200 instrument and quantity determined by qPCR using an NEBnext library quantification kit (NEB). Libraries were sequenced as described previously46 and reads are available from ArrayExpress using accession code E-MTAB-11906 ...
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bioRxiv - Cancer Biology 2023Quote: ... Quality control and quantification of the resulting dual-indexed barcoded libraries was performed with Agilent TapeStation and by qPCR (NEBNext Library Quant Kit for Illumina, New England Biolabs).
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bioRxiv - Plant Biology 2023Quote: ... The reactions were completed in a final volume of 10 µl using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs), and relative gene expression analysis using the Livak method was per-formed 53 ...
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bioRxiv - Cancer Biology 2023Quote: ... Eluted DNA was used for qPCR or sequencing library preparation with NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... 5′ FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1 3′) and the Luna Universal Probe one-step RT-qPCR kit (catalog no. E3006; New England Biolabs). A 20-μL RT-qPCR mixture contained 7 μL of sample ...
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bioRxiv - Genomics 2023Quote: ... The polymerase employed in our qPCR stop assays was exonuclease and proof-reading activity deficient Klenow fragment (M0212L; New England Biolabs). The Control DNA (Fig 3A ...
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bioRxiv - Neuroscience 2023Quote: Quantitative real-time PCR (qRT-PCR) was used to measure RNA with Luna Universal One-Step RT-qPCR Kit (New England Biolabs) via CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Dual-indexing was carried out with the TruSeq panel of indexing primers in a short-cycle PCR using Luna Universal Probe One-Step RT-qPCR reagents (NEB). The pooled adapter libraries were purified and size-selected using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (New England Biolabs) and the primers listed in Suppl ...
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bioRxiv - Microbiology 2024Quote: ... the RNA pellet was resuspended in 50 µl of RNase-free H2O and 1 µl of the resuspended samples was used for quantification of the DENV-2 D220 NS5 RNA regions using Luna Universal One-Step RT-qPCR Kit (New England Biolabs) in a 10-ul reaction volume ...
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bioRxiv - Genetics 2021Quote: ... Ribonucleoprotein injection mix was prepared with 1.30 μl of Cas9 enzyme (20 μM, New England BioLabs), 1.60 μl of prepared gRNAs ...
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bioRxiv - Genomics 2021Quote: ... 0.1μL of 100μM Smart-seq2 Oligo-dT primer (SigmaAldrich) and 1μL dNTP mix (10mM each, NEB). In P1 ...
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bioRxiv - Developmental Biology 2022Quote: ... 5µl of the PCR mix was digested overnight with 5 units of BbsI (New England Biolabs) in 30 µl volume ...
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bioRxiv - Developmental Biology 2019Quote: ... Stained cell solution was diluted in a mix with diluent and RNase inhibitor (New England Biolabs) to 1 cell/50 nl for dispensing on the ICELL8-chip (Takara ...
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bioRxiv - Genetics 2019Quote: ... and the mix was ligated to each sample using T4 RNA ligase I (New England BioLabs). Every sample was treated with the same mix of 5’ adapters ...
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bioRxiv - Microbiology 2020Quote: ... 1µL Random Primer Mix (ProtoScript II First Strand cDNA Synthesis Kit, New England Biolabs, https://www.neb.ca) was added to 7 µL extracted RNA and denatured on SimpliAmp thermal cycler (ThermoFisher Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... between the HindIII and EcoRI restriction sites using NEBuilder HiFi DNA Assembly mix (New England Biolabs). The full sequences of all plasmids were verified as previously described (21,49) ...
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bioRxiv - Genomics 2021Quote: ... DNA samples were labeled with 10 μL of a cocktail of repair enzymes (PreCR mix, NEB), fragmented ...
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bioRxiv - Genomics 2019Quote: Genomic control DNAs were digested to nucleosides by treatment with the Nucleoside Digestion Mix (NEB, M0649S) for 1 h at 37 C ...
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bioRxiv - Microbiology 2021Quote: ... End repair and dA-tailing were performed using NEBNext FFPE Repair Mix (New England BioLabs, 6630) and NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... 10 ml of T7 enzyme mix and 12.5 ml of 5’GpppA cap analog (S1406S, NEB) in a final reaction volume of 100 ml for 30 min at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The 20 µl reaction mix containing 80 ng of single-stranded M13mp18 DNA (New England Biolabs), 10 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2019Quote: ... were mixed with 1µl of 10mM dNTPs mix (LifeTechnologies) and 1µl of 50µM random hexaprimers (NEB). Samples were incubated at 65°C for 5 minutes ...