Labshake search
Citations for New England Biolabs :
4601 - 4650 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Exonuclease I mix (20 μL of 10X Exonuclease I buffer (New England Biolabs, Ipswich, MA, USA), 10 μL of Exonuclease I (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... Next, 680 nL of ligation mix (1.47x T4 ligase reaction buffer, 5.88 mM ATP (NEB, P0756L), 140 U T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2022Quote: ... we followed the protocol described by the Template Switching RT Enzyme Mix provider (New England Biolabs) with a few modifications (Supplementary Materials and Methods) ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... 2 µL of cDNA mix was added to 50 µL Phusion High-Fidelity DNA Polymerase (NEB) reaction mix ...
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bioRxiv - Immunology 2021Quote: ... 500 ng total RNA was used with the Template Switching RT Enzyme Mix (NEB Ipswich, USA) to generate 5’ RACE cDNA ...
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bioRxiv - Immunology 2021Quote: ... 2 µL of random primer mix 60 µM (cat. n° S1330S, NEB, New England Biolabs, USA) and 4.8 µL of nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... 2 µL of random primer mix 60 µM (cat. n° S1330S, NEB, New England Biolabs, USA) and 4.8 µL of nuclease-free water ...
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bioRxiv - Genomics 2021Quote: ... dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1 ...
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bioRxiv - Genomics 2021Quote: dATP was added to the 3’ ends by adding 9μL of A-tailing mix (5μL NEB buffer 2.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction product was combined with 20 μl digestion mix containing 5 μl ExoI (NEB, M0293S), 5 μl HinFI (NEB ...
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bioRxiv - Immunology 2020Quote: ... the reaction mix was used as a template in an in vitro translation reaction (PURExpress, NEB). Constructs bearing mRNA – comprising of (i ...
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bioRxiv - Genomics 2022Quote: ... a 2 μl mix of 6.4 U of Exo I (New England Biolabs, Ipswich, MA, USA), 32 U of Exo III (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... complementary DNA (cDNA) was synthesized using ProtoScript® II Reverse Transcriptase with random primer mix (NEB). This cDNA was also used for a conventional PCR using Go-Taq® Master Mix (Promega ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the ligation mix was directly used to transform 10-beta competent Escherichia coli cells (NEB), to amplify pSG01 ...
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bioRxiv - Systems Biology 2023Quote: ... the flow cell was incubated with Exonuclease I mix (1 U/uL Exo I (NEB, M0293L) in 1x Exo I buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... The sections were then incubated in a ligation mix (1× T4 Rnl2 reaction buffer (NEB, B0239SVIAL), 0.05 µM RCA primer ...
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bioRxiv - Microbiology 2024Quote: ... the isolated DNA was processed to single nucleosides by applying Nucleoside Digestion Mix (NEB, MA, USA).
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bioRxiv - Microbiology 2024Quote: ... the linear plasmid obtained by PCR was ligated using the KLD enzyme mix (NEB cat # M0554S) according to the manufacturer’s protocol before transformation ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 100 µL of CIP-mix containing 10 U of calf intestinal alkaline phosphatase (New England Biolabs) in CIP-buffer were added and beads incubated for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... and deleting the eGFP open reading frame using site-directed mutagenesis with KLD enzyme mix (NEB). Ectopic complementation vectors were cloned onto pSEC1 (derivative of pK-PIM integrating vector ...
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bioRxiv - Microbiology 2024Quote: ... Tags (6x His or HA) were introduced using site-directed mutagenesis with KLD enzyme mix (NEB).
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bioRxiv - Cancer Biology 2023Quote: ... the samples were end-repaired by adding 2 μl NEBNext FFPE DNA Repair Mix (NEB, M6630) and 3 μl NEBNext Ultra II End Prep Enzyme Mix (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... The genes were diluted to 125 pM in 30 µL PURExpress cell-free expression mix (NEB) containing 0.06 % pluronic acid (F-127 ...
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bioRxiv - Molecular Biology 2022Quote: ... we extended the primer to generate double-strand DNA with Klenow Large fragment mix (1X NEB Buffer 2 ...
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bioRxiv - Genomics 2023Quote: ... lacZ reporter gene and H11 locus homology arms using NEBuilder HiFi DNA Assembly Mix (NEB, E2621). The sequence identity of donor plasmids was verified using Nanopore sequencing (Primordium Labs) ...
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bioRxiv - Physiology 2023Quote: ... and 1µg RNA was transcribed into cDNA using LunaScript RT Super Mix (E3010L, New England Biolabs). Afterwards the mRNA levels were determined by qPCR using SYBR Green (Luna Universal qPCR Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... The HA protein tag and point mutations were introduced using the KLD Enzyme mix (NEB M0554S) and were verified by Sanger sequencing and whole-plasmid sequencing (Plasmidsaurus).
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bioRxiv - Genomics 2023Quote: ... the pellet was resuspended in 200 μl of ligation mix (1x T4 ligation buffer (NEB, B0202S), 50 Units T4 DNA ligase (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: All plasmids were assembled via Gibson assembly using the HiFi DNA Assembly mix (New England Biolabs), unless stated otherwise ...
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bioRxiv - Cancer Biology 2023Quote: ... and library preparation performed using NEBNext® FFPE DNA Repair Mix (New England Biolabs® Inc) and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (New England Biolabs® Inc) ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 µL PCR Anchor Primer,1 µL Deoxynucleotide (dNTP) Solution Mix (New England Biolabs, Inc., N0447L), 10 µL Q5® Reaction Buffer (New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR products were then isolated via gel electrophoresis and assembled using HiFi assembly mix (NEB #E2621L). Plasmids were verified by DNA sequencing and stored at -20°C until use in NanoBRET assays.
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bioRxiv - Microbiology 2023Quote: ... 200 ng of poly(A)+ RNA were digested with a nucleoside digestion mix (New England Biolabs) at 37°C for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate-antibody mix was then incubated with Magnetic Protein A beads (New England Biolabs, S1425S) overnight at 4 °C with end over rotation ...
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bioRxiv - Cancer Biology 2023Quote: ... we prepared a digestion mix containing 100 nL of NlaIII restriction enzyme (NEB, cat. no. R0125L) and 50 nL of CutSmart buffer (NEB ...
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bioRxiv - Genomics 2023Quote: ... 2.0 μg of the HMW DNA was treated with the NEBNext FFPE DNA Repair Mix (NEB) and the NEBNext UltraII End Repair/dA-Tailing Module (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were digested in Digestion Pre-Mix (VIZGEN 20300005) and RNase inhibitor (New England Biolabs M0314L) for 3 h at 37°C and then cleared for 16 to 24 hours with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003 ...
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bioRxiv - Biophysics 2024Quote: ... PCR was followed by the addition of the PCR product to a KLD enzyme mix (NEB) for template removal ...
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bioRxiv - Developmental Biology 2019Quote: ... The cDNA was amplified and fused to the coding region for Neon Green by Gibson assembly (NEB-E2611). This fusion gene was inserted into pUAST-attb using added Xho1 and Xba1 sites present in pUAST-attb and added to kkv::NG during construction using PCR and oligos containing the sites ...
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bioRxiv - Microbiology 2020Quote: The real-time PCR technique was performed for NDV detection using Luna Universal One-Step RT-qPCR Kit E3005E (New England BioLabs, USA) following the manufacture procedures ...
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bioRxiv - Microbiology 2021Quote: ... 3 µl of extracted RNA was used in a TaqMan one-step qRT/PCR assay (Luna® Universal One-Step RT-qPCR Kit, NEB). TaqMan analysis was carried out with primer/probe combination described in Table 1 and analysis performed on the QuantStudio™ 6 Flex System (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... Expression of mRNA transcripts for PfRFC1 gene analysis were carried out using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Inc.), on a QuantStudio 5 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... Spike-in RNA of the bacteriophage MS2 served as an interal control and was detected with Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) using corresponding primers and probe (MS2_F ...
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bioRxiv - Bioengineering 2021Quote: ... qRT-PCR was performed with primers specific for target genes (see Supplementary Table 1 for the list of primers) using the Luna universal One-Step RT-qPCR kit (NEB; E3005). Experiment was performed using the QuantStudio3 (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... qRTPCR was performed with primers mentioned in Table S3 with Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs). Log2 normalized RNA abundances were calculated using ValidPrime signal as reference (51).
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bioRxiv - Microbiology 2021Quote: ... qRT-PCR was performed either directly on the inactivated supernatants or on extracted RNA using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005E) in a QuantStudio 6 thermocycler (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... 100 ng total RNA per 20 µl reaction were analyzed using the Luna Universal Probe One-Step RT-qPCR Kit (NEB, # E3006) in technical triplicates ...
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bioRxiv - Plant Biology 2022Quote: ... and LCM cell sample collections (ME, TAP, and OSC) was performed using the Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs). Quantitative PCR was performed using TaqMan primers synthesized by Integrated DNA Technologies (Table S2 ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of the replication of each mutant versus the reference was performed using Luna® Universal Probe One-Step RT-qPCR kit (New England BioLabs) containing 3uL of total RNA ...
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bioRxiv - Microbiology 2021Quote: ... Yields of viral RNA were quantified by real-time qPCR by using SARS-CoV-2 specific primers targeting the E gene with the Luna®Universal One-Step RT-qPCR Kit (New England Biolabs) in a LightCycler 480 thermocycler (Roche ...