Labshake search
Citations for New England Biolabs :
3651 - 3700 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... each sample was mixed with 2x RNA loading dye (New England Biolabs, UK). And the ssRNA ladder (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... 2X hybridisation buffer (4X SSC, 20% dextran sulphate, 2 mg/mL BSA (NEB), 1/10 volume nuclease free water and 1/10 volume vanadyl-ribonucleoside complex (VRC ...
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bioRxiv - Microbiology 2024Quote: ... the dsDNA duplex with 2x parS sites digested with XhoI and SalI (NEB) was ligated into the plasmid with 3x parS site digested with XhoI and dephosphorylated ...
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bioRxiv - Biochemistry 2023Quote: ... and SNAP-Cell Oregon Green (NEB).
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bioRxiv - Microbiology 2021Quote: ... Total RNAs were used as templates for SYBR green-based one-step reverse-transcriptase quantitative PCR (RT-qPCR) using the NEB Luna One-Step RT-qPCR kit (New England Biolabs). All primers were validated by standard curve analysis and had PCR efficiencies ranging from 90-110% ...
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bioRxiv - Bioengineering 2020Quote: ... We added 1 μL of the resulting DNase digestions to 10 μL RT-qPCR reactions (Luna One-Step Universal RT-qPCR Kit, New England Biolabs) containing 0.8 U/μL RNasin Plus (Promega ...
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bioRxiv - Microbiology 2021Quote: ... 4μL purified RNA were subsequently used for RT-qPCR reaction using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) and 0.4μM of each primer ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of mRNA for TRAP complex subunits were estimated by RT-qPCR using Luna Universal One-Step RT-qPCR Kit (NEB) following total RNA isolation with RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... RNA was then diluted to 20 ng/uL and 20-60 ng of RNA was used for qPCR using Luna universal one-step RT-qPCR kit (NEB) as per manufacture’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA copies were quantified in 10% of the obtained eluate volume with a one-step RT-qPCR reaction using a standard curve and the Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) and previously published TaqMan primers and probe (Corman et al. ...
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bioRxiv - Genetics 2020Quote: ... The RNA was then diluted 1:50 and 2 mL were used to perform a one-step qPCR protocol using Luna Universal One-step qPCR kit (NEB). Two primer sets were used ...
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bioRxiv - Immunology 2020Quote: ... We quantified RNA using a one-step RT qPCR reaction with the NEB Luna Universal Probe One-Step RT-qPCR kit (New England Biolabs) and previously published TaqMan primers and probes (SARS-CoV-2 E_Sarbeco and hamster RPL18 ...
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bioRxiv - Microbiology 2022Quote: ... qPCR reactions were performed on an Applied Biosystems 7500 Real-Time PCR system using the Luna Universal qPCR MasterMix (NEB) with the primers listed in (Table S3) ...
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bioRxiv - Microbiology 2022Quote: Detection of viral genomes from heat-inactivated samples was performed by RT-qPCR using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) with SARS-CoV-2 specific primers targeting the N gene region (5′-TAATCAGACAAGGAACTGATTA-3′ and 5′-CGAAGGTGTGACTTCCATG-3′ ...
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bioRxiv - Genetics 2023Quote: ... RT-qPCR was performed on 10 ng total RNA using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005L). bin3 ...
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bioRxiv - Bioengineering 2023Quote: RT-qPCR was carried out from the isolated RNA using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs) following the manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The Ct values of samples were determined by quantitative PCR (qPCR) using a Luna Universal Probe One-Step RT-qPCR Kit (cat. #E3006, New England Biolabs) with LightCycler 480 (Roche Diagnostics ...
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bioRxiv - Cell Biology 2023Quote: ... Viral RNA quantification was performed by RT-qPCR using the IP4 set of primers and probe and the Luna Universal Probe One-Step RT-qPCR Kit (NEB). Serial dilutions of a titrated viral stock were analyzed simultaneously to express viral loads as eqPFU per gram of tissue.
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bioRxiv - Systems Biology 2023Quote: ... RT-qPCR quantification was carried out on 20 ng of RNA with the Luna One-Step RT-qPCR Kit (NEB). All qPCRs were performed in technical triplicates ...
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bioRxiv - Immunology 2024Quote: ... Gene expression levels were quantified by reverse transcription quantitative PCR (RT-qPCR) using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs), and being conducted on a QuantStudio 3 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... and random primer mix (NEB) before amplification by the ARTIC Network protocol using the multiplexed primer scheme version 3 ...
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bioRxiv - Plant Biology 2020Quote: ... random primer mix (NEB #S1330S) and M-MLV Reverse transcriptase (Invitrogen #28025-013 ...
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bioRxiv - Microbiology 2022Quote: ... 10 mm NTP mix (NEB), Ribolock (Thermo scientific) ...
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bioRxiv - Genetics 2019Quote: ... and random primer mix (NEB). Aliquots were then made of the experimental working concentration dilutions of cDNA to reduce freeze-thaw cycles ...
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bioRxiv - Synthetic Biology 2020Quote: ... and Random Primer Mix (NEB) for quantification by RT-qPCR with SYBR Green Master Mix ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 μl dNTP mix (NEB), 0.2 μl MgCl2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... random primer mix (NEB #S1330S) and M-MLV Reverse transcriptase (Invitrogen #28025-013 ...
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bioRxiv - Biophysics 2022Quote: ... 1 µL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Biophysics 2022Quote: ... 2 µL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein Deglycosylation Mix II (NEB) was added and further incubated for 30 min ...
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bioRxiv - Genetics 2024Quote: ... and 10mM dNTP mix (NEB). All qPCR reactions were carried out on an Applied Biosystems Step One plus real-time PCR system using standard thermal-cycling conditions optimised for KAPA SYBR® FAST qPCR Mix ...
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bioRxiv - Bioengineering 2024Quote: ... deglycosylation mix II (P6044S NEB) was obtained from New England Biolabs (NEB) ...
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bioRxiv - Biophysics 2023Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S subunit ...
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bioRxiv - Developmental Biology 2021Quote: ... and quantified using qPCR (Kapa Biosystems or NEB). Libraries were pooled for multiplex sequencing ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed using Luna Universal Mastermix (NEB), denaturation/annealing/elongation was performed for 50 cycles ...
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bioRxiv - Cancer Biology 2022Quote: ... Luna Universal qPCR MasterMix (NEB, cat. no. M3003) was used according to manufacturer’s specifications ...
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bioRxiv - Neuroscience 2023Quote: ... using the Luna qPCR Mastermix (New England Biolabs) and normalized to Gapdh housekeeping gene ...
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bioRxiv - Microbiology 2022Quote: ... were mixed with 2.5 µl Low Range ssRNA Ladder (New England Biolabs), 25 µl 2⨯ GLII loading buffer and 22.5 µl water and then denatured like the sample ...
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bioRxiv - Molecular Biology 2024Quote: ... along with the low range ssRNA ladder (New England Biolabs, cat# N0364S), were used to guide size-fractionation ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA preparations were then used directly for qPCR (200 ng total RNA per reaction) using the Luna Universal One-Step RT-qPCR Kit (NEB, E3006) and target-specific primer/probe sets (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: ... from COLO205 cells was subjected to one-step RT-qPCR analysis using the Luna Universal One-Step RT-qPCR Kit (NEB, E3005S) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and gene expression was analyzed by RT-qPCR on QuantStudio 6 (Applied Biosciences) using the Luna Universal qPCR kit (New England Biolabs, M3003). Relative expression was normalized to Actb and Gapdh housekeeping genes and was determined using the ΔΔCt method ...
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bioRxiv - Molecular Biology 2022Quote: The rest of RNAs were performed with real-time reverse transcription PCR (RT-qPCR) using a Luna® Universal Probe One-Step RT-qPCR Kit (New England Biolabs) according to the manufacturer’s recommendations by a thermocycler (BIO-RAD CFX96™ Real-Time System) ...
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bioRxiv - Microbiology 2022Quote: ... 1-step RT-qPCR was performed on 2μL of RNA using the Luna® Universal One-Step RT-qPCR Kit (NEB Biosciences) and primers for β-tubulin (F ...
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bioRxiv - Microbiology 2022Quote: ... primers AngiCOI_forward (S0963) and AC1R (S0966) were used in a qPCR reaction with Luna® Universal qPCR Mastermix (New England Biolabs, Australia). The qPCR reactions were run on the CFX96 Touch Real-Time PCR Detection System (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR reactions were carried out in 10 μL reactions using the Luna® One-Step Universal RT-qPCR kit (NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... RNA was diluted 1:50 for RT-qPCR which was conducted using the Luna Universal One-Step RT-qPCR kit (New England Biolabs E3005E) on a C1000 touch thermal Cycler / CFX384 Real-Time System (Biorad) ...
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bioRxiv - Microbiology 2023Quote: RNA was used as template to detect and quantify viral genomes by duplex reverse transcriptase (RT) quantitative polymerase chain reaction (RT-qPCR) using a Luna Universal Probe one-step RT-qPCR kit (New England Biolabs, E3006E). SARS-CoV-2-specific RNAs were detected by targeting the ORF1ab gene using the following set of primers and probes ...
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bioRxiv - Genomics 2022Quote: ... mScarlet and HPRT (housekeeping gene for normalization) levels were then measured by qPCR using the Luna Universal One-Step RT-qPCR Kit (NEB #E3005) with 100ng total RNA per reaction ...