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Citations for New England Biolabs :
3051 - 3100 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... A second primer was then added and a complementary mutated bottom strand synthesised using Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) and Taq DNA ligase ...
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bioRxiv - Genetics 2023Quote: ... the region containing the target surrounded by the context was amplified by PCR using primers P7-P8 with Q5 Hot Start High-Fidelity 2× Master Mix (NEB) with the following conditions ...
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bioRxiv - Genetics 2023Quote: Purified PCR products and enzyme digested plasmid were then ligated together in a gibson reaction using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacturer’s protocol resulting in pUC19-3xP3-DsRed-attB-Gypsy-MCS-no-BbsI ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNA cassettes were isolated by two rounds of PCR using a previously published strategy (17) with NEBNext Ultra II Q5 Master Mix (New England Biolabs) and primer pairs listed in Extended Data Table 2.
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated by 5-7 rounds of PCR amplification using NEBNext High-Fidelity 2× PCR Master Mix (NEB, M0541L), purified using SPRIselect reagent kit (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µL of RNA from 4 x 96 well plates was transferred to a 384 well plate with 3 µL of master mix containing 1 µL of a 10 mM stock of dNTPs (NEB Catalog# N0447L ...
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bioRxiv - Molecular Biology 2023Quote: ... The ligated dsDNA products were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs) with 200 nM of Illumina multiplex and index barcode primers (98°C for 10 sec pre-denaturation followed by 15 cycles of 98°C for 5 sec ...
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bioRxiv - Molecular Biology 2023Quote: The extracted DNA was used as a template to amplify the variable region by a two-step PCR strategy using a high-fidelity DNA polymerase (NEBNext ultra II Q5 master mix, M0544L, NEB). The first PCR of 22-24 cycles was performed using primers #232 to #273 (Table S9 ...
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bioRxiv - Immunology 2023Quote: PCR was performed in a 40 μl PCR reaction containing 1x Q5 HotStart Hifi PCR master mix (New England Biolabs), 0.4 μM of Illumina adapter PCR primers (ILM_p5_PCR ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids encoding phage proteins were constructed by amplification of the desired genes from the phage genomes and assembly into the pBbA6c backbone using NEBuilder HiFi DNA Assembly Master Mix (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA libraries were amplified with 16 cycles of PCR using the NEBNext Ultra II Q5 Master Mix (provided) and unique index primer mixes from NEBNext Multiplex Oligos for Illumina Library (#E6609L, New England Biolabs). Libraries were purified with 0.9× SPRISelect Beads (#B23318 ...
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bioRxiv - Molecular Biology 2023Quote: ... then adapter ligation was carried out using the sequencing adapters supplied with the kit and NEB Blunt/TA Ligase Master Mix (New England Biolabs).
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bioRxiv - Developmental Biology 2023Quote: ... 2 μl of indexed P7 (Cao et al., 2017) and 20 μl of NEBNext High-Fidelity master mix (New England Biolabs) were added to each well and PCR performed as follows ...
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bioRxiv - Genetics 2023Quote: ... 50 µl PCR reactions were performed for each timepoint using Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) with 20 to 50ng of template isolated plasmid DNA ...
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bioRxiv - Biochemistry 2022Quote: ... from which a 500 bp dsDNA containing the λC31 attB at its center was generated by PCR using Phusion High Fidelity PCR Master Mix from NEB. After heat denaturation ...
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bioRxiv - Bioengineering 2023Quote: ... were synthesized from Genewiz (Suzhou) and cloned into the linearized PXZ vector by Gibson assembly using Gibson Assembly Master Mix (New England Biolabs). pegRNA plasmids were constructed by using the plasmid (Addgene #132778 ...
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bioRxiv - Cancer Biology 2023Quote: ... The sgRNA cassette was PCR amplified from genomic DNA using Phusion High-Fidelity PCR Master Mix (New England Biolabs, M0531S). The amplified products were pooled and amplified again via PCR using primers harboring Illumina TruSeq adapters with i5 and i7 barcodes ...
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bioRxiv - Genomics 2022Quote: ... the resulting products were firstly ligated with barcodes from the Native Barcoding Expansion kits by using NEB Blunt/TA Ligase Master Mix (New England Biolabs) and then with adapter from the Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Immunology 2023Quote: ... The CAR backbone constructs were ordered as gBlock (IDT) and cloned into the LZRS-IRES-eGFP retroviral plasmid by Gibson Assembly (NEBuilder HiFi DNA Assembly Master Mix) (NEB). The VHH specific sequence was amplified using PCR (Phusion High Fidelity PCR kit ...
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bioRxiv - Cell Biology 2023Quote: ... and PCR amplified for 7-9 cycles depending on the samples using NEBNext High Fidelity 2× PCR master mix (New England Biolabs). The optimal cycle number was determined empirically each time by qPCR ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The DNA and DNA Assembly Master Mix were incubated at 50°C for 20 min and then NEB Stable Competent Cells (New England Biolabs) were transformed with the product ...
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bioRxiv - Systems Biology 2023Quote: ... The DpnI-treated linear plasmid backbones were then mixed with the relevant PCR amplified tile and assembled by in vitro recombination with the NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs). The assembly reactions were purified ...
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bioRxiv - Biochemistry 2023Quote: The generation of all plasmids was performed using NEBuilder HiFi DNA assembly Master Mix (New England Biolabs GmbH, Frankfurt, Germany). Protein coding sequences (CDS ...
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bioRxiv - Genomics 2023Quote: ... were PCR-amplified (2 PCR reactions, 12 cycles) using Illumina adapter-specific primers and NEBNext Ultra II Q5 Master Mix (NEB). After library profile analysis by Agilent 2100 Bioanalyser (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR fragments were assembled using NEBuilder® HiFi DNA Assembly Master Mix (Cat. No. E2621, NEB, Ipswich, MA, USA). DNA sequences in all these plasmids were authenticated by automatic sequencing.
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bioRxiv - Synthetic Biology 2023Quote: ... was replaced with a Renilla luciferase CDS via Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) to generate a dual-luciferase reporter containing luc2P and Renilla expression cassettes (pGL4.33R) ...
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bioRxiv - Bioengineering 2023Quote: ... variable-region sequences were ordered as gBlock DNA fragments (Integrated DNA Technologies) and NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) was used to clone these into the pcDNA3.4 (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... PCR products were purified and cloned into human-IgG (Heavy, Kappa or Lambda) expression plasmids32 using the Gibson Assembly Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... inserts with homology overhangs for cloning were ordered from IDT and cloned into the XbaI-and EcoRI-digested pPB-Zeocin backbone using HiFi DNA Assembly Master Mix (NEB). To prepare plasmids for AAV production ...
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bioRxiv - Genetics 2023Quote: ... Inserts were cloned into the NotI-and EcoRI-digested pCMV-PE2 backbone using HiFi DNA Assembly Master Mix [New England Biolabs (NEB)] ...
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bioRxiv - Genomics 2023Quote: ... we amplified the library using PCR with a 10 µl reaction mixture containing 5 µl of Phusion High Fidelity MASTER Mix (New England Biolabs), 2 µl of P1 primer (AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC G) ...
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bioRxiv - Cell Biology 2023Quote: Transgene constructs were assembled in the pAP088 backbone [2] for single-copy insertions near the ttTi4348 site on Chr I using NEBuilder Hifi DNA assembly Master Mix (New England Biolabs). The egl-20 coding sequence in pAP209 was codon-optimized using the C ...
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bioRxiv - Biophysics 2023Quote: HeR-48C12 was amplified from pBAD-Helios-NT-6xHis and combined with TSX3ER2 and Citrine using overlap-extension PCR with Phusion high fidelity master mix (NEB). The primers used for cloning are listed in the Supporting Information (SI ...
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bioRxiv - Microbiology 2023Quote: ... V3-V4 region of 16S rRNA gene was amplified using specific primers with the barcode and Phusion High-Fidelity PCR Master Mix (New England Biolabs). PCR products were mixed at equal density ratios ...
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bioRxiv - Biochemistry 2023Quote: ... Vector and insert fragments were amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF buffer from New England Biolabs (NEB). The fragments were analyzed by agarose gel electrophoresis and template DNA was digested using DpnI ...
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bioRxiv - Systems Biology 2023Quote: ... and used as the template for PCR with primers containing gene-specific targeting homology arms (1x Q5 Master Mix, New England Biolabs #M0494S ...
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bioRxiv - Microbiology 2023Quote: ... We then cloned these sequences into the SphI-XbaI digested suicide vector pDM4 (50) by Gibson assembly using NEBuilder HiFi Assembly master mix (New England Biolabs), transformed into E ...
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A modular circuit architecture coordinates the diversification of courtship strategies in DrosophilabioRxiv - Neuroscience 2023Quote: ... 1 Kb homology arms beginning at the predicted Cas9 cut sites in ppk23 were PCR amplified with Q5 High-Fidelity master mix (NEB) and cloned into pIM174 ...
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bioRxiv - Genomics 2023Quote: ... Barcodes were amplified from cDNA or input plasmid DNA using 24 cycles of PCR with Phusion High Fidelity Master Mix (NEB). Amplicons were purified with the Monarch PCR kit (NEB) ...
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bioRxiv - Synthetic Biology 2023Quote: All PCR and ligation reactions were performed with OneTaq polymerase and Gibson Assembly Master Mix from New England Biolabs (NEB), respectively ...
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bioRxiv - Bioengineering 2024Quote: ... inserts with homology overhangs for cloning were ordered from IDT and cloned into the pPB-Zeocin backbone using HiFi DNA Assembly Master Mix (NEB). All PCRs were performed using Q5 High-Fidelity DNA Polymerase (NEB).
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bioRxiv - Microbiology 2023Quote: PCR reactions were performed by 30μL reactions using 15μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs, USA), 0.2 μM of primers
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bioRxiv - Microbiology 2023Quote: ... individual barcodes were incorporated into the dA-tailed DNA using the EXP-NBD104 and EXP-NBD114 native barcoding expansion kit following the ONT protocol with NEB Blunt//TA Ligase Master Mix (New Englands Biolabs). Barcoded DNA samples were then equimolarly pooled ...
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bioRxiv - Microbiology 2023Quote: ... 1.2 kb of homology regions to the chromosomal sequence flanking the up- and down-stream of deletion or insertion sites were inserted into the pVoPo-04 backbone or pVoPo-05 backbone by Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, New England BioLabs). 5-10 μg of the constructed plasmids were dialyzed and electroporated into F ...
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bioRxiv - Molecular Biology 2023Quote: ... thanks to restriction sites flanking the to-be-cloned region or by Gibson assembly (using the NEBuilder Hifi DNA assembly master mix, NEB). pFD115 was obtained by replacing the RBS region and gfp gene of pFD086 (24 ...
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bioRxiv - Genomics 2023Quote: ... cDNA libraries were amplified with 16 cycles of PCR using the NEBNext Ultra II Q5 Master Mix (provided) and unique index primer mixes from NEBNext Multiplex Oligos for Illumina Library (#E6609L, New England Biolabs). Libraries were purified with 0.9× SPRISelect Beads (#B23318 ...
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bioRxiv - Microbiology 2023Quote: ... using JLHP836/838 and cloned into BamHI and NheI digested pDSW1278 (15) by Gibson assembly (16) using NEBuilder HiFi DNA Assembly Master Mix (NEB). This plasmid was conjugated into C ...
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bioRxiv - Microbiology 2023Quote: ... HMBS_232 was cloned into pCMV-puroR lentiviral plasmid using the primers listed in Table S6 and NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs). Lentiviruses were produced as already mentioned ...
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bioRxiv - Neuroscience 2023Quote: ... as wildtype or T205A variant were amplified by polymerase chain reaction (PCR) using Q5 High-Fidelity master mix (M0492, New England Biolabs (NEB)) from previous plasmid constructs 16 ...
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bioRxiv - Biochemistry 2023Quote: ... A Streptomyces coelicolor overexpression plasmid containing AlbAB was constructed via Gibson assembly by mixing the AlbAB-encoding gBlock and a pGM1190 vector digested with NdeI and BamHI restriction enzymes with Gibson assembly master mix (NEB) for 20 minutes at 50°C ...