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Citations for New England Biolabs :
2151 - 2200 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... PCR was performed using High-Fidelity Phusion PCR Master Mix (NEB, Frankfurt am Main, Germany) (initial denaturation (98 °C ...
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bioRxiv - Cell Biology 2021Quote: ... The DNA segments were PCR amplified (Phusion High-Fidelity PCR Master Mix, NEB, UK, #M0531) and fused with Gibson recombination cloning method (Gibson Assembly Master Mix ...
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bioRxiv - Microbiology 2020Quote: ... pre-amplified using PreAmp Master Mix (Fluidigm) and treated with exonuclease I (New England Biolabs). Samples were then mixed with SsoFast EvaGreen Supermix with Low ROX (Biorad) ...
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bioRxiv - Cell Biology 2021Quote: ... from all the samples and amplified by PCR using Q5 master mix (New England Biolabs) and forward-TGTGTCAATGGAACTCTTGAC and reverse-TCGTTTCGACACCGAAGCAG primers.
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bioRxiv - Plant Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA, USA). Transformations were carried out using 2 µL of 4x diluted assembly mixes into DH5α cell lines ...
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bioRxiv - Synthetic Biology 2022Quote: ... Plasmids were constructed by Gibson Assembly using NEBuilder® HiFi DNA Assembly Master Mix (NEB) or ligation using T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... were cloned into a pCAGGS vector backbone using NEBuilder HiFi DNA Assembly Master Mix (NEB). To generate an LCMV strain Cl13-GP (Genbank ...
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bioRxiv - Immunology 2022Quote: ... VP2 and VP3 with high-fidelity Phusion HiFi PCR Master Mix (NEB, Ipswich, MA, USA) using specific primers (S6 Table) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... and Phusion® High-Fidelity PCR Master Mix with HF buffer (New England Biolabs Inc). The amplification was performed in 50 μL PCR reactions that contained:
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bioRxiv - Developmental Biology 2022Quote: ... Then, 2.5 μL of MNase master mix (MNase Buffer, 0.125 U/μL MNase (NEB, M0247S), 2 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... B1-B2 fragments for Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB E2621S) to generate pLKO.1-WT-Paxillin-T2A-GFP ...
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bioRxiv - Cell Biology 2022Quote: ... RAD18 knockout positive cells were confirmed with PCR using Q5 High Fidelity Master Mix (NEB), sanger sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ChIP-seq libraries were prepared with NEBNext ChIP-Seq Library Prep Master Mix (NEB, E6240L) and then amplified with NEBNext High-Fidelity 2X PCR Master Mix (NEB ...
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bioRxiv - Immunology 2021Quote: ... using the high-fidelity Master mix Q5 (cat. n° M0492S, NEB, New England Biolabs, USA), and the primers NDV-3LS1-2020-F1 (5’-GATCATGTCACGCCCAATGC-3’ ...
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bioRxiv - Immunology 2021Quote: ... using the high-fidelity Master mix Q5 (cat. n° M0492S, NEB, New England Biolabs, USA), and the primers NDV-3LS1-2020-F1 (5’-GATCATGTCACGCCCAATGC-3’ ...
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bioRxiv - Neuroscience 2021Quote: ... These fragments were used for Gibson assembly using Gibson Assembly Master Mix (New England Biolabs). PCR products were produced with the Q5 High-Fidelity 2X Master Mix (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... sgRNA-cloning was performed with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621S) according to manufacturer’s instructions using BbsI-linearization of PX458 ...
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bioRxiv - Immunology 2022Quote: PCRs were done with the Q5 Hot-start 2× master mix (New England BioLabs, NEB), and cloning was performed using the Gibson Assembly 2× Master Mix (NEB ...
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bioRxiv - Immunology 2022Quote: PCRs were done with the Q5 Hot-start 2× master mix (New England BioLabs, NEB), and cloning was performed using the Gibson Assembly 2× Master Mix (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled with Gibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). Assembled DNA was transformed into competent cells prepared by the CCMB80 buffer (TekNova ...
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bioRxiv - Molecular Biology 2022Quote: ... Gibson assembly was performed with these products using the Gibson assembly master mix (NEB E2611) with an incubation time of 20 minutes room temperature ...
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bioRxiv - Genetics 2019Quote: ... and amplified by PCR using Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) to produce sub-pools for Gibson assembly (NEB) ...
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bioRxiv - Genomics 2019Quote: cDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) using input amounts determined from qPCR ...
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bioRxiv - Genomics 2019Quote: cDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) using volumes determined from qPCR distributed across 5 replicates each ...
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bioRxiv - Genomics 2019Quote: ... gDNA was amplified with NEBNext Q5 Hot Start HiFi PCR Master Mix (New England Biolabs) with a biotinylated reverse-transcriptase primer Creb_Hand_RT_3 and a biotinylated primer specific to luciferase (Creb_Luc_Seq_R) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The spiralen promoter and mCherry gene were amplified using Q5 master mix (New England Biolabs), with the plasmid pTF20mChloxp (gift from Kevin Dybvig ...
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bioRxiv - Genomics 2019Quote: ... were ligated to the end-prepared DNA fragments using Blunt/TA Ligase Master Mix (NEB), followed by clean-up using 0.4x v/v AMPure XP beads and sequencing kit reagents ...
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bioRxiv - Synthetic Biology 2021Quote: ... and was made suitable for GGA cloning using Gibson assembly (NEB Gibson Assembly Master Mix) of PCR products of the vector (NEB Phusion High-Fidelity DNA Polymerase ...
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bioRxiv - Molecular Biology 2021Quote: ... and the resulting library was PCR amplified using Q5 Ultra II Master Mix (NEB, # M0544S). The library was size selected by agarose gel electrophoresis and column purified (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and cloned into pDONR/Zeo (Thermo) by Gibson Assembly (NEB HiFi DNA Assembly Master Mix) and then moved into pARC8-DEST for arabinose-inducible expression ...
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bioRxiv - Microbiology 2021Quote: ... The assembly was achieved using the NEBuilder HiFi DNA Assembly master mix (New England Biolabs), using a miniaturized reaction that includes 50 ng of each DNA fragment and 2 μL of the master mix in a 6 μL reaction ...
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bioRxiv - Microbiology 2021Quote: ... Gibson assembly was done using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) with purified DNA fragments according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... pDL1201 (Figure S3) by blunt-end ligation (Blunt/TA Ligase Master Mix, New England Biolabs). Mutations in gp23 or gp25 were cloned into separate plasmids ...
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bioRxiv - Microbiology 2019Quote: ... These fragments were ligated using the Gibson Assembly Master Mix (New England Biolabs, Ipswich MA), and reamplified using primers JR143 and JR146 ...
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bioRxiv - Microbiology 2019Quote: ... All constructions were assembled using the Gibson Assembly Master Mix (New England Biolabs, Ipswich, MA), and then each was reamplified with nested primers containing SacI restriction site (primers SacI-F and SacI-R ...
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bioRxiv - Immunology 2021Quote: ... gel purified and ligated with prepared oligos using Instant Sticky-end Ligase Master Mix (NEB). After bacterial transformation ...
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bioRxiv - Microbiology 2020Quote: ... Both PCR products were then assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB), resulting in VHp693.
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bioRxiv - Developmental Biology 2021Quote: ... a PCR was undertaken on yw67 fly gDNA using a Q5 polymerase master mix (NEB) and the following primer pair (preceded by the T7 promoter sequence ...
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bioRxiv - Developmental Biology 2021Quote: ... The backbone and insert were combined by Gibson Assembly Master Mix (New England Biolabs, E2611L). The gRNA plasmid was confirmed by sequencing and injected into transgenic embryos expressing Vasa-Cas9 and allowed to develop to adulthood (BestGene Inc. ...
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bioRxiv - Genetics 2021Quote: ... and Gibson assembled with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Cat. # E2621). Resulting plasmids were transformed into NEB 5-alpha chemically-competent E ...
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bioRxiv - Genetics 2020Quote: ... and Gibson assembled with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Cat. # E2621). Plasmids were transformed into NEB 5-alpha chemically-competent E ...
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bioRxiv - Genetics 2020Quote: ... The 10 μl reactions contained 5 μl Q5 High-Fidelity Master Mix (New England Biolabs), 1 μl forward primer [10 μM] ...
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bioRxiv - Genomics 2020Quote: ... gRNA oligonucleotides were annealed and extended using Phusion High Fidelity DNA polymerase master mix (NEB) with thermal cycling conditions of 98°C two minutes (1x) ...
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bioRxiv - Microbiology 2020Quote: ... Gibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich, MA) was followed to ligate the amplified fragments with the expression vector pBAD33 (80) ...
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bioRxiv - Genetics 2019Quote: ... cloning of donor vectors was performed with the Gibson Assembly Master Mix (New England Biolabs). PCR products were produced with the Q5 High-Fidelity 2X Master Mix (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... and Phusion® High-Fidelity PCR Master Mix with HF buffer (New England Biolabs Inc) (see Table S1 for further details) ...
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bioRxiv - Genomics 2020Quote: ... Gibson assembly reactions were carried out using NEBuilder HiFi DNA Assembly Master Mix (NEB E2621L) with DNA fragments containing homology arms between 15 and 20 base pairs long ...
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bioRxiv - Microbiology 2022Quote: ... PCR master mix reagents (see Figure 4): Taq polymerase and 10X Reaction Buffer (NEB M0273S), nucleotide mix containing 10 mM dTTP ...
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bioRxiv - Developmental Biology 2022Quote: ... and subcloned into pCAGGS_Myc plasmid using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Individual ASO-targeted sequences were mutated by inverse PCR with PrimeSTAR GXL DNA polymerase (TaKaRa ...