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Citations for New England Biolabs :
2051 - 2100 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The donor cassettes were assembled using the NEBuilder HiFi DNA Assembly Master Mix (NEB) and were designed to leave the 5’UTR and 3’UTR of the Copg1 gene intact ...
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bioRxiv - Microbiology 2021Quote: ... and inserted to the intermediate plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB). The L-sNTurboID fragment was ligated back to the pCEZ-L backbone using the same restriction sites Pac1 and Hpa1 by NEB T4 ligase.
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bioRxiv - Molecular Biology 2021Quote: ... or fragment assembly using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and NEBuilder HiFi DNA Assembly Master Mix (E2621L, New England BioLabs, Ipswich, MA, USA). All constructs were verified by Sanger sequencing and midiprepped for electroporation and/or transfection using PureLink Fast Low-Endotoxin Midi Kit (A36227 ...
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bioRxiv - Biophysics 2020Quote: ... The resulting PCR product was subsequently inserted using Gibson Assembly Master Mix (NEB, E2611L) into the lentiviral vector CD813A (System Biosciences ...
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bioRxiv - Genomics 2020Quote: ... and RT-PCR was performed with Phusion High Fidelity DNA polymerase master mix (NEB) each with the manufacturer recommended PCR thermal cycles ...
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bioRxiv - Genomics 2020Quote: ... Samples were dissolved in 10 μl of master mix for fragmentation (consisting of NEB next First Strand Synthesis ...
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bioRxiv - Neuroscience 2021Quote: ... RILPL1-miniSOG-HA construct was generated using Gibson Assembly Master Mix (New England Biolabs), and identity of construct verified by sequencing of the entire coding region ...
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bioRxiv - Microbiology 2021Quote: ... using NEBuilder High Fidelity (HiFi) DNA Assembly Master Mix (New England Biolabs, Ipswich, MA) via Gibson Assembly Protocol (68) ...
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bioRxiv - Genetics 2020Quote: ... amplified using primers with KpnI and BamHI RE sites with Phusion Master Mix (NEB), digested ...
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bioRxiv - Genomics 2021Quote: ... Tables S1 and S7) using NEBNext Ultra II Q5 Master Mix (New England Biolabs). Each reaction (100 µL total volume ...
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bioRxiv - Genomics 2020Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB) with minor modifications and a custom automated protocol on a Biomek FX (Beckman)25 ...
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bioRxiv - Cell Biology 2021Quote: ... cloned into NdeI cut pET28a using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S), single colonies picked and Sanger sequenced.
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bioRxiv - Cancer Biology 2021Quote: ... using the NEBuilder Hi Fi DNA Assembly Master Mix (New England Biolabs, Beverly, MA). For the production of lentiviral particles ...
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bioRxiv - Cell Biology 2022Quote: ... The cloning reactions were performed using the Gibson Assembly Master Mix (New England Biolabs). Site-directed mutagenesis was performed with the Quik-Change kit (Agilent) ...
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bioRxiv - Microbiology 2022Quote: ... both fragments being assembled with NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs). Catalytic mutants msrDE125Q ...
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bioRxiv - Genomics 2022Quote: ... The enriched DNA fragments were further amplified using NEBNext Q5 Master Mix (NEB, M0544) and NEBNext Library Quant Primer Mix (NEB ...
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bioRxiv - Genomics 2022Quote: ... then ligated to a custom hairpin adapter using NEB ligase master mix (NEB, M0367). The incomplete ligation product (fragment having only one or no adaptor ligated ...
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bioRxiv - Microbiology 2022Quote: ... Gibson reactions were carried out according to manufacturer’s recommendations (NEB, Gibson Assembly master mix). This was transformed into electrocompetent E ...
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bioRxiv - Plant Biology 2022Quote: ... and self-assembled using Gibson assembly (NEBuilder® HiFi DNA Assembly Master Mix, NEB).
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bioRxiv - Microbiology 2022Quote: ... and 25 μL of NEB Blunt/TA Ligase Master Mix (New England Biolabs, UK). The mixture was incubated at room temperature for 10 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, 1 h 50° C) into backbones previously digested with corresponding restriction enzymes (NEB ...
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bioRxiv - Microbiology 2022Quote: ... Secondary amplification reactions were performed using NEBNext high-fidelity 2 PCR master mix (NEB) in 50 μl reactions (26 μl of 2X mix ...
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bioRxiv - Cell Biology 2022Quote: ... sgRNA inserts were PCR-amplified using NEBNext High Fidelity PCR Master Mix (NEB, M0541). The resulting PCR amplicons were purified using Ampure XP Beads and sequenced using the HiSeq 2500 system (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified and cloned using Gibson assembly master mix (New England BioLabs) into LRG3.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... For PCRs we used Phusion High-Fiedlity PCR Master Mix with HF Buffer (NEB) and reactions were run as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... and introduced to pCS2+ vector using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621). The H3-tail sequence (Shindo & Amodeo ...
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bioRxiv - Immunology 2024Quote: ... sample amplification was performed using a library PCR using Q5 Master Mix (M0544L, NEB), 1 µL i7 Index primer (Sigma-Aldrich®) ...
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bioRxiv - Microbiology 2024Quote: ... and pNJ-26 vector were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB), to generate the plasmid pSAG1:EGFP-DHFR-BAG1:mCherry ...
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bioRxiv - Bioengineering 2024Quote: ... 500 µL PCR reagents containing 1.67X Q5® High-Fidelity Master Mix (NEB, M0515), 0.625 mg/mL BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Linearized cDNA was PCR amplified using Phusion High-Fidelity PCR Master Mix (NEB, M0531S).
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bioRxiv - Genomics 2023Quote: ... The homology vector was cloned by Gibson assembly (Gibson Assembly Master Mix kit, NEB) into a restriction-enzyme digested pCAG backbone plasmid ...
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bioRxiv - Genetics 2023Quote: ... Plasmid assemblies were achieved either with NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621) or T4 DNA Ligase (M0202) ...
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bioRxiv - Microbiology 2022Quote: The second cDNA strands were generated with LongAmp Taq Master Mix (New England Biolabs). The ends of the double-stranded cDNAs were repaired with NEBNext End repair /dA-tailing Module (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR was performed using the Quick-LoadR©Taq2×Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using Phusion 5X Master mix (New England Biolabs GmbH, M0492S). For a 25 µl reaction ...
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bioRxiv - Biochemistry 2023Quote: ... Cloning was performed using either NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) or standard restriction cloning methods ...
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bioRxiv - Neuroscience 2023Quote: ... Gibson Assembly was performed using NEB HiFi Gibson Assembly Master mix (NEB Catalog# E2621L). Gibson Assembly reaction products were transformed into NEB Turbo high efficiency chemically competent cells (NEB Catalog# C2984I ...
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bioRxiv - Neuroscience 2023Quote: ... Gibson Assembly was performed using NEB HiFi Gibson Assembly Master mix (NEB Catalog# E2621L). The scFv Gibson Assembly reaction products were transformed into NEB Turbo high efficiency chemically competent cells (NEB Catalog# C2984I ...
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bioRxiv - Synthetic Biology 2023Quote: ... Reactions closely followed instructions from the NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621S). Fragments were amplified with PCR and designed to have 20-30 bp overlaps ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed utilizing the Phusion High-Fidelity PCR Master Mix (NEB, M0531L) to prevent any mutations ...
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bioRxiv - Microbiology 2023Quote: ... and ligated into pET17b vector using the Gibson Assembly Master Mix (New England Biolabs). The ligation products were transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... and ligated into the pET17b plasmid randomly using the Gibson Assembly Master Mix (NEB). The transformation was performed in the same manner as the above.
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bioRxiv - Immunology 2023Quote: ... tagmented DNA was amplified using NEBNext High-Fidelity PCR Master Mix (New England BioLabs) with the following primers ...
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bioRxiv - Cell Biology 2023Quote: ... Assembly was done with NEBuilder HiFi DNA Assembly Master Mix (E2621L, NEW ENGLAND BioLabs). Correct insertion of the amplicon was confirmed by sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs catalog # M0531), and/or LongAmp® Hot Start Taq DNA Polymerase with Expand HF Buffer (Roche catalog # 05917131103 ...
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bioRxiv - Genomics 2022Quote: ... 16 µl RNA were mixed with 4 µl LunaScript master mix (NEB cat# E3010L), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (#M5520AA, New England Biolabs, Ipswich, MA, USA). For the construction of the SNV-Env expression plasmid (phCMV3-SNV-Env) ...
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bioRxiv - Genetics 2023Quote: ... 5% DMSO and 1× NEBNext High-Fidelity PCR master mix (New England BioLabs, M0541L) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... and subjected to Gibson Assembly using NEBuilder HiFi DNA Assembly Master Mix (NEB, E2621L) following the manufacturer’s instructions ...