Labshake search
Citations for New England Biolabs :
2001 - 2050 of 5111 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The fragments were then assembled using NEBuilder HiFi DNA assembly master mix (NEB) to place the cassette sequence positioned in the middle ...
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bioRxiv - Microbiology 2024Quote: ... was performed using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs). The ligation product was transformed into DH10B E.coli bacteria by heat shock ...
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bioRxiv - Genomics 2020Quote: ... Five microliters from the ONT Adapter Mix (AMX) and 50 μl of Blunt/TA Ligation Master Mix (New England Biolabs) were mixed with each sample ...
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bioRxiv - Biophysics 2022Quote: ... The resultant mix was combined with 10 nM adapter and an equal volume of TA ligase master mix (New England Biolabs). The mix was centrifuged at 4°C for 1 min and then incubated at room temperature for 20 min ...
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bioRxiv - Plant Biology 2020Quote: ... Transcript knockdown was confirmed in T0 and T1 plants using qPCR (qScript cDNA synthesis kit, QuantaBio®, and Luna qPCR mix; NEB®) according to manufactuers’ protocols ...
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bioRxiv - Biochemistry 2023Quote: ... both the Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019) and the Luna Universal Probe One-Step RT-qPCR Kit (NEB E3006 ...
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bioRxiv - Genomics 2020Quote: ... The reaction mixture was composed of a PCR ready mix (using NEBNext® Ultra™ II Q5® Master Mix, NEB), lysate product and a final primer concentration of 1uM each ...
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bioRxiv - Genomics 2023Quote: ... Beads were washed with 200 μl buffer EB and resuspended in 400 μl of Post-TdT PCR mix (1x NEBNEXT Ultra II Q5 Master Mix (NEB, M0544L), 0.5 μM post-TdT-poly(C)12-S Primer ...
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bioRxiv - Neuroscience 2023Quote: ... 10µL ligation mix was prepared (20ng digested pX552 vector, 0.8µL 1:50 diluted oligo mix, 5µL 2X T7 Buffer (BioLabs #B0318S), 0.5µL T7 DNA Ligase (BioLabs #M0318S ...
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bioRxiv - Cell Biology 2020Quote: ... The NanoLuciferase gBLOCK and homology arms were joined using Gibson assembly master mix (NEB) and transformed into Stbl3 bacteria ...
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bioRxiv - Cell Biology 2022Quote: ... vector by Gibson assembly111 using the NEBuilder® HiFi DNA Assembly Master Mix (NEB). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... and 15 μL of 2× Phusion High-Fidelity PCR Master Mix (New England Biolabs). All PCR amplification reactions were carried out on a T100 Thermal Cycle (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 25 μL of NEB Next High-Fidelity PCR Master Mix (2×) (NEB, Ipswich, USA), 1 μL of Universal PCR Primer (25 mmol ...
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bioRxiv - Microbiology 2019Quote: ... Gibson reaction was performed using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) according to the manufacture’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Library amplification was carried out with NEBNext Ultra II Q5 Master Mix (NEB #M0544), RP1 common primer ...
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bioRxiv - Physiology 2019Quote: ... PCR was performed using Phusion Hot Start Flex 2× Master Mix (NEB, Frankfurt, Germany) in a GeneAmp PCR system 9700 (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: The gel extracted DNA was amplified using the NEBNext PCR Master Mix (NEB#M0541S) with custom primers (WSO398 and WSO381 ...
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Bioengineering 2019Quote: Phosphorylation master mix (5x): 1 mM Adenosine 5’-Triphosphate (ATP, New England Biolabs, NEB), 0.1 U/µL T4 Polynucleotide Kinase (T4 PNK ...
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bioRxiv - Cancer Biology 2020Quote: All Gibson assemblies were performed using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) and were assembled at 50 °C for 60 min at appropriate molar ratios ...
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bioRxiv - Cancer Biology 2020Quote: Libraries were prepared using NEBNext® ChIP-Seq Library Prep Master Mix (E6240, NEB) and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S ...
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bioRxiv - Plant Biology 2020Quote: ... The fragment was re-assembled using NEBuilder® HiFi DNA Assembly Master Mix (NEB). Constructs were transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... Libraries were then amplified with Q5 High-Fidelity Master Mix (M0492S, NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Systems Biology 2021Quote: 25 ul - Phusion High-Fidelity PCR Master Mix with HF Buffer (New England Biolabs)
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bioRxiv - Microbiology 2021Quote: ... All PCRs of flanking regions were carried out using Phusion-HF Master Mix (NEB) according to the manufacturer’s protocol with an annealing temperature (Ta ...
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bioRxiv - Cell Biology 2021Quote: ... and fused with Gibson recombination cloning method (Gibson Assembly Master Mix, NEB, UK, #E2611) in pEGFP-N3 empty backbone ...
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bioRxiv - Microbiology 2021Quote: ... by Gibson assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, USA). This vector was named pDAR25-Kan ...
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bioRxiv - Plant Biology 2020Quote: ... using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs Inc., UK, pE2B-MpC1HDZmut). Later ...
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bioRxiv - Genomics 2021Quote: ... The library was assembled using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) in 4 × 20 μL reactions at 50 °C for 1 hour using 100 ng of vector per 5-10 ng of PCR amplicon ...
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bioRxiv - Cell Biology 2021Quote: ... DNA constructs were either generated using the Gibson Assembly Master Mix (New England Biolabs), or the T4 DNA ligase (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and cloning was performed using Gibson Assembly 2× Master Mix (New England BioLabs, NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The reactions were performed using the Multiplex 5X Master Mix (New England Biolabs, Inc.) in a final volume of 12.5 μl following the protocol described by the manufacturer ...
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bioRxiv - Synthetic Biology 2022Quote: ... and assembled withGibson assembly protocol using NEBuilder HiFi DNA Assembly Master Mix(NEB, E2621S). Assembled DNA was transformed into competent cells prepared by the CCMB80 buffer (TekNova ...
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bioRxiv - Synthetic Biology 2022Quote: Constructs were assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB, catalogue no. E2621L). Reactions consisting of approximately equal amounts of the common fragment and the variable fragment were prepared using the FeliX liquid handler or electronic multi-channel pipette ...
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bioRxiv - Microbiology 2022Quote: ... Amplicons were detected with Luna® Universal qRT-PCR Master Mix (New England Biolabs) in a CFXConnect Real-Time PCR Instrument (Bio-Rad Laboratories) ...
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bioRxiv - Molecular Biology 2020Quote: All plasmids were cloned using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and mutations were made using the Q5 Site-directed mutagenesis kit (New England Biolabs) ...
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bioRxiv - Bioengineering 2021Quote: ... Reactions were performed with Phusion High-Fidelity Master Mix and GC buffer(NEB, US) using 1 to 30 ng of plasmid DNA templates ...
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bioRxiv - Synthetic Biology 2022Quote: ... by isothermal assembly using the HiFi DNA Assembly Master Mix (New England Biolabs, USA). Following assembly ...
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bioRxiv - Cell Biology 2019Quote: ... Library was amplified using NEBNext High-Fidelity 2× PCR Master Mix (New England Biolabs). Library concentration was measured by DNA High Sensitivity Kit (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... and cloned into an SEC backbone plasmid (NEBuilder HiFi DNA Assembly Master Mix, NEB). The modified Cas9/sgRNA plasmid ...
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bioRxiv - Synthetic Biology 2020Quote: ... 6 μl primers (10 μM) and 50 μl Q5 high fidelity master mix (NEB). The thermocycling parameters were ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were constructed using the NEBNext Ultra DNA Sample Prep Master Mix Kit (NEB), with minor modifications and a custom automated protocol on a Biomek FX (Beckman) ...
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bioRxiv - Genomics 2021Quote: ... (Grevet et al., 2018) using NEBuilder HiFi DNA Assembly master mix (NEB, Cat #E2621X) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... which included 25 µL of NEBNext Ultra II Q5 master mix (NEB, Cat #M0544L), 1.25 µL of the 10 µM forward primer and 1.25 µL of the 10 µM reverse primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or NEBNext Q5 HotStart HiFi PCR Master Mix (New England BioLabs, Ipswich, MA, USA) for eight to 14 cycles ...
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bioRxiv - Cell Biology 2020Quote: ... effectors were then inserted into the vector using the Gibson Assembly Master Mix (NEB).
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bioRxiv - Physiology 2020Quote: ... was amplified by PCR (Phusion High-Fidelity PCR Master Mix, NEB, Hitchin, Hertfordshire, UK) using specific oligonucleotide primers (5’-TCGCTACTGTTTCTCTCGCA-3’ and 5’-AAAGGCGTCAACAACTGCTT-3’) ...
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bioRxiv - Biophysics 2020Quote: ... The two DNAs were ligated using a Gibson Assembly Master Mix (New England Biolabs) and subcloned into the pWMD01 vector (33) ...
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bioRxiv - Cell Biology 2021Quote: ... all sgRNAs were amplified using Phusion Flash High-Fidelity PCR Master Mix (NEB, M0531L) with the primers ...