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Citations for New England Biolabs :
2051 - 2100 of 2232 citations for Rat BRSK1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the corresponding ORFs were amplified from the WT strain 536 and cloned into the pUC18 plasmid (ampicillin 100 μg/mL) using BamHI and SphI enzymes (New England Biolabs, USA) (ECP_3022 ...
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bioRxiv - Biophysics 2022Quote: ... Both insert and vector were digested using the said restriction enzymes and ligated to form a circular plasmid using T4 DNA ligase (NEB, #M0202L). Sequence was verified by sanger sequencing.
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bioRxiv - Cell Biology 2022Quote: All plasmids reported in this work were constructed using NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs Cat. #E2621), based on the Gibson assembly method (Gibson et al. ...
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bioRxiv - Cell Biology 2022Quote: ... the PCR product and the pDRF1-GW plasmid were digested using BamHI-HF and NheI-HF (New England Biolabs, Ipswich, MA) and the PCR product was ligated into the plasmid using T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were designed in SnapGene and mutants were generated via Gibson assembly (Gibson Assembly® Cloning Kit from New England Biolabs)57,58 or via QuickChange (Q5® Site-Directed Mutagenesis Kit from New England Biolabs).59 Recombinant expression of motors in E ...
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bioRxiv - Immunology 2022Quote: Point mutations were introduced through linearizing the plasmid by PCR with primers containing the intended mutations (Supp. Table S1) followed by reverting to circular plasmid with Gibson Assembly Master Mix (M5510A, NEB, USA).
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bioRxiv - Microbiology 2023Quote: An integration cassette containing two regions of homology flanking a kanamycin-resistance gene was engineered on a pUC backbone plasmid encoding ampicillin resistance using the NEBuilder HiFi DNA Assembly mastermix (NEB E2621L). A counter-selectable marker based on PheS77 was engineered under the constitutive SpeI promoter and cloned into the pUC plasmid (generating pUC-BC ...
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bioRxiv - Molecular Biology 2022Quote: RLuc-P2A-X-P2A-Fluc plasmids (where X represents a variable sequence) were assembled using NEB Builder HiFi DNA Assembly Cloning Kit (New England Biolabs, #E2621S) by combining the original plasmid digested with HindIII (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated by deletion of the unwanted DNA from the parental plasmid using the Q5 Site-Directed Mutagenesis Kit (NEB BioLabs). The R763G point mutation was introduced into D1-A1 by PCR mutagenesis ...
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bioRxiv - Neuroscience 2022Quote: ... and was ligated with 500 ng of the purified digested plasmid band and then transformed in NEB stable competent cells (New England BioLabs, C3050H). Successful ligation of the guide was confirmed by Sanger sequencing using a mU6 sequencing primer (Supplementary Table 7).
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bioRxiv - Physiology 2022Quote: To identify the molecular lesion in the hyds-2 strain a 9KB PCR fragment of the ZK795.1 gene was cloned into the PUC19 plasmid by Gibson assembly (New England Biolabs, Ipswich, MA). The seaEx22 plasmid along with a myo-2:mCherry (PCFJ90 ...
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bioRxiv - Microbiology 2023Quote: ... All enzymes for DNA amplification (PHUSION® polymerase) and plasmid construction (restriction enzymes, T4-DNA ligase and Quick CIP) were obtained from New England Biolabs (NEB) and used according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... with primers listed in Table S8, then inserted into PHR-mCh- CryWT plasmid (Adgene, 101221) by using NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S). The generated constructs were fully sequenced to confirm the absence of any mutations or stop codons ...
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bioRxiv - Systems Biology 2023Quote: ... HiFi-Cas9 and LZ3-Cas9 constitutive expression plasmids lentiHiFi-Puro and lentiLZ3-Puro were constructed by the Gibson Assembly Site-Directed Mutagenesis approach (NEB, #E2621) using lentiCas9-Puro as the template ...
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bioRxiv - Synthetic Biology 2023Quote: ... The purified barcode fragment was then assembled into the cloning backbone plasmid pKI110 by Golden Gate Assembly (66) using BsmBI (NEB #R0580S). We performed two assembly reactions ...
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bioRxiv - Synthetic Biology 2023Quote: ... and the plasmids for the Blasticidin and Zeocin resistance marker-based systems were introduced to T7 Express chemically competent cells (NEB #C2566I) according to the manufacturer’s high-efficiency transformation protocols ...
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bioRxiv - Synthetic Biology 2023Quote: ... Linear plasmid fragments were generated by PCR and purified by Monarch® PCR and DNA cleanup kit (New England BioLabs®). Ligations were performed with In-Fusion® enzyme at 50°C for 15 minutes (min ...
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bioRxiv - Synthetic Biology 2023Quote: ... we performed the ligation of fragments from module plasmids in the presence of BsaI-HF (New England BioLabs, Ipswich, MA, USA) to generate array plasmids ...
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bioRxiv - Microbiology 2022Quote: ... Fragments were integrated into the linearized (KpnI/XbaI digested) pRK415 plasmid by NEBuilderR HiFi assembly cloning method (NEB Lab, United States) as described above.
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bioRxiv - Molecular Biology 2022Quote: PANK3 mutations were made in a gateway compatible pDONR plasmid containing a PANK3 ORF (a gift from the lab of Ben Cravatt) by amplifying the whole plasmid with primers containing the desired mutations and using HiFi DNA Assembly Master Mix (NEB, # E2621) to re-circularize the amplicon ...
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bioRxiv - Microbiology 2023Quote: ... Phosphorylated primers containing one half of the barcode each at the 5’ ends were used to linearize the pUC-BC plasmid and the resulting amplicon was ligated using T4 DNA Ligase (NEB M2200L). The purified ligation reaction was transformed into NEB-10-Beta competent cells (NEB C3020K ...
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bioRxiv - Microbiology 2023Quote: Plasmid construction was performed by: PCR amplification of the fragment to insert in the plasmid with Q5® High-Fidelity DNA Polymerase (New England Biolabs), digestion with the appropriate FastDigest restriction enzymes (ThermoFisher) ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 μg of linearized plasmid was used as template in a 10 µL reaction using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040S) with an 8:1 ratio of cap analog to GTP ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid assembly was performed by in vitro Gibson Assembly using a HiFi DNA Assembly master mix (New England Biolabs, Ipswich, MA), downscaled to 5 µL reaction volumes ...
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bioRxiv - Bioengineering 2023Quote: ... The components of the integration plasmids pTargetF_araA::P103/105/106/101/100-SIDF were sequentially assembled into pTargetF_araA GG using BsaI-HFv2 (NEB, Ipswich, MA, USA) digestion in four steps ...
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bioRxiv - Neuroscience 2023Quote: ... we introduced a silent mutation into the PAM motif of the sgRNA located within the 3’ homology arm in the donor plasmid by using Q5 Site-Directed Mutagenesis kit (NEB, E0054). The donor plasmid was confirmed with DNA sequencing ...
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bioRxiv - Microbiology 2023Quote: ... For in vitro transcription plasmids were first linearised by restriction digest and purified using Monarch PCR and DNA Clean-up Kit (NEB, USA). 3’UTRs were in vitro transcribed from 500ng of plasmid DNA using MEGAscript T7 Transcription Kit (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... which were then used to replace the corresponding regions in the wildtype plasmids with NEBuilder® HiFi DNA Assembly Cloning Kit (New England BioLabs). All plasmids were fully sequenced ...
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bioRxiv - Biophysics 2023Quote: ... These plasmids were digested with NotI-HF and XhoI for 2 h at 37°C (R3189, R0146, New England Biolabs, UK) and heat-inactivated for 20 min at 80°C.
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bioRxiv - Bioengineering 2023Quote: ... a pair of BbsI enzymatic digestion sites were designed to set just after the MAD7 crRNA in the ST7 expression plasmid for the cloning of annealed crRNA oligos by restriction cloning with BbsI and T4 ligase (New England Biolabs, USA). crRNA candidates were designed with CHOPCHOP (Labun et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Ligation of the plasmid with 3Xty insert was carried out via Gibson assembly as per manufacturer’s instructions (New England Biolabs; catalog # M5520AA2) and transfected into NEB5alpha bacteria ...
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bioRxiv - Developmental Biology 2023Quote: ... The lineage barcode oligo mix was cloned downstream of the Read2 partial primer sequence in the purified donor plasmid via multiple Gibson Assembly reactions (NEB, E2611S). Gibson assembly reactions were then pooled and desalted with 0.025 µm MCE membrane (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Mixing the linearised pCRISPR-cBEST plasmid and Del-ptaA with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, USA). The linearised pCRISPR-cBEST plasmid was then bridged by Del-ptaA ...
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bioRxiv - Genomics 2022Quote: ... We then ordered an oligo containing 16 Ns with flanking homology arms to the landing pad plasmid (GWLP P1) and used HiFi Assembly (NEB #E2621) to assemble the oligo to the plasmid (50℃ ...
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bioRxiv - Genomics 2022Quote: ... we digested the respective plasmids with AgeI and EcoRI and ligated them to the backbone with T4 DNA Ligase (NEB #M0202). For ALOXE3 and ALOXE3-mut ...
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bioRxiv - Genomics 2022Quote: ... signal constructs were amplified by PCR from different plasmids and assembled using the NEBuilder HiFi DNA Assembly Master Mix (HiFi Assembly, NEB #E2621). The BxbI attP site was then added using the Q5 Site-Directed Mutagenesis Kit (Q5 SDM ...
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bioRxiv - Genomics 2022Quote: ... In vitro methylation of the plasmid with and without the SOCS3 insert was carried out using M.SssI methylase enzyme (NEB, cat.no: M0226S) following standard protocols and successful methylation of the insert was confirmed by digestion with methylation sensitive restriction enzymes AfeI ...
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bioRxiv - Developmental Biology 2023Quote: ... pCS2-dKif6 (1-501)-EGFP was generated from the pCS2-dKif6-EGFP plasmid by Gibson assembly (NEB HiFi DNA Assembly Kit) using a GeneArt gene fragment encoding amino acids 1-501 (Thermo Fisher).
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bioRxiv - Cancer Biology 2022Quote: ... The pLentiCRISPRv2 plasmid was digested for 1 hr at 55°C with 1U per μg of DNA BsmBI-v2 (NEB, R0580), in 5 μl Buffer 3.1 and to a final volume of 50 μl ...
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bioRxiv - Biochemistry 2023Quote: ... was used to linearize plasmid (10 µg) by incubating at 37 °C for a minimum of 2 hours in 1x CutSmart buffer (NEB, B7204S). Linearized plasmid was purified by extraction with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 ...
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bioRxiv - Cancer Biology 2022Quote: ... HRH1 and CHRM3 CRISPR/Cas9 knockout plasmids were constructed from LentiCRISPRv2E plasmids by first phosphorylating and annealing paired sgRNAs with 10X T4 ligation buffer (New England Biolabs, #B0202S) and T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Developmental Biology 2023Quote: ... This plasmid was modified to include a C-terminal V5 tag using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For arnt1-myc ...
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bioRxiv - Bioengineering 2023Quote: ... Plasmid assembly was performed by in vitro Gibson assembly using a HiFi DNA Assembly Master Mix (New England Biolabs, Ipswich MA), downscaled to 5 µL reaction volume ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Oligonucleotides were ligated into pSp-Cas9-(BB)-2A-GFP plasmid that had been linearised by digestion with BbsI-HF (NEB R3539) and gel purified (Bioline BIO-52060 ...
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bioRxiv - Cell Biology 2023Quote: ... APEX2-ATP7B-EGFP and APEX2-mKO2-HA-ATP7A constructs were made on the existing plasmid using NEBuilder HiFi DNA Assembly (NEB #E2621). Mutations on eGFP-ATP7B were prepared following Q5 Site-Directed Mutagenesis Kit (NEB #E0554 ...
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bioRxiv - Microbiology 2023Quote: ... using a minimum of 20 bp overlapping regions between DNA fragments with custom made kit Plasmid selection and verification after the transformation were checked using the OneTaq® 2X Master Mix with Standard Buffer (NEB). Plasmids were purified using the Qiagen plasmid purification kit according to manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: ... The purified products were cloned into pWW plasmid series upstream of sfGFP/mCherry coding sequences37 using NEBuilder® HiFi DNA Assembly (NEB). To perform co-expression experiments ...
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bioRxiv - Microbiology 2023Quote: ... Four independent mutant plasmid libraries were constructed by mutagenizing mraY in plasmid pNG93 (PlacUV5::mraY) using Taq polymerase with Thermopol buffer (New England Biolabs, M0267L). The forward 5’- ACACTTTATGCTTCCGGCTC-3’ and reverse 5’- ACTGTTGGGAAGGGCGATCAAA-3’ primers were used to amplify mraY from pNG93 ...
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bioRxiv - Synthetic Biology 2023Quote: ... reaction was performed to remove sfGFP and replace it with the cassette components (P1-FLAG, P2-Substrate, P3-HA, P4-ERS). This plasmid was transformed into competent Escherichia coli (E. coli) (NEB#C2984H), purified (QIAGEN #27106 ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid length DNA binding experiments were performed with unlabeled circular or linearized M13mp18 single (New England Biolabs, M13mp18 single-stranded DNA) or double-stranded DNA (New England Biolabs ...